ライフサイエンスコーパス: chaffeensis

1 is a tick-borne disease caused by Ehrlichia chaffeensis.

2 -specific expression of the T4S system in E. chaffeensis.

3 iable-length PCR target (VLPT) protein in E. chaffeensis.

4 the protein expression and interaction in E. chaffeensis.

5 -containing vacuoles than in vacuole-free E. chaffeensis.

6 nsis-containing vacuoles and vacuole-free E. chaffeensis.

7 ular basis of the host immune response to E. chaffeensis.

8 by a tick-transmitted rickettsia, Ehrlichia chaffeensis.

9 ost cell-specific antigenic expression by E. chaffeensis.

10 e disease caused by the rickettsia Ehrlichia chaffeensis.

11 was developed for the detection of Ehrlichia chaffeensis.

12 ared with previously described assays for E. chaffeensis.

13 ritical for conferring rapid clearance of E. chaffeensis.

14 d T-cell function on murine resistance to E. chaffeensis.

15 a major antigenic protein (P28) of Ehrlichia chaffeensis.

16 are critical for conferring resistance to E. chaffeensis.

17 e disease caused by the rickettsia Ehrlichia chaffeensis.

18 membrane proteins (OMPs) of E. canis and E. chaffeensis.

19 zoonoses caused by infection with Ehrlichia chaffeensis.

20 f both the IL-8 promoter and NF-kappaB by E. chaffeensis.

21 olysaccharide-deficient bacterium, Ehrlichia chaffeensis.

22 obligately intracellular bacterium Ehrlichia chaffeensis.

23 at D. melanogaster is a suitable host for E. chaffeensis.

24 _0379 mutant and challenge with wild-type E. chaffeensis 1 month following inoculation with the mutan

25 spectrometry identified the protein as an E. chaffeensis 12.3-kDa hypothetical protein, which was des

26 l tandem repeat DNA-binding domain in the E. chaffeensis 120-kDa tandem repeat protein (TRP120) that

27 olymerase chain reaction (PCR) for Ehrlichia chaffeensis, 2) acute and convalescent serum titers, and

28 genes and that antigenic variation of the E. chaffeensis 28-kDa proteins may result from differential

29 Recently, molecular interactions between E. chaffeensis 47-kDa tandem repeat (TR) protein (TRP47) an

30 . canis and the corresponding ortholog of E. chaffeensis (47 kDa) were identified and the proteins ch

31 01 were positive by PCR assays for Ehrlichia chaffeensis (50 of 217; 23%), Ehrlichia ewingii (44 of 2

32 Major immunoreactive proteins in E. chaffeensis (75-kDa) and E. canis (95-kD) whole-cell lys

33 d a very high prevalence of antibodies to E. chaffeensis (97 of 112; 87%) and a low prevalence of ant

34 Ehrlichia chaffeensis, a bacterium that cannot survive outside the

35 totropic ehrlichiosis is caused by Ehrlichia chaffeensis, a Gram-negative bacterium lacking lipopolys

36 Ehrlichia chaffeensis, a tick-borne rickettsial organism, causes t

37 Ehrlichia chaffeensis, a tick-transmitted rickettsial agent, cause

38 Ehrlichia chaffeensis, a tick-transmitted rickettsial agent, is re

39 Ehrlichia chaffeensis, a tick-transmitted rickettsial, is the caus

40 E. ewingii accounted for 20 (91%), and E. chaffeensis accounted for 1 (5%) of the positives.

41 Third, E. chaffeensis also inhibited the gene transcription of RAB

42 ning, infectious disease caused by Ehrlichia chaffeensis, an obligate intracellular bacterium that la

43 d mutations by allelic exchange in Ehrlichia chaffeensis, an obligate intracellular tick-borne bacter

44 Ehrlichia chaffeensis, an obligate intracellular, tick-transmitted

45 The genome of Ehrlichia chaffeensis, an obligatory intracellular bacterium that

46 Ehrlichia chaffeensis, an obligatory intracellular gram-negative b

47 ns with nonviral pathogens (2 with Ehrlichia chaffeensis and 1 with Mycoplasma pneumoniae).

48 Analysis of E. chaffeensis and A. phagocytophilum genome sequences reve

49 btained for HL-60 cells used as hosts for E. chaffeensis and A. phagocytophilum.

50 ested whether cholesterol is required for E. chaffeensis and A. phagocytophilum.

51 Ehrlichia chaffeensis and Anaplasma phagocytophilum are agents of

52 olymerase chain reaction assay for Ehrlichia chaffeensis and by the demonstration of morulae within p

53 Ehrlichia chaffeensis and E. canis have a small subset of tandem r

54 re observed primarily in the periplasm of E. chaffeensis and E. canis.

55 Only Ehrlichia chaffeensis and E. ewingii have been thought to cause eh

56 e bond formation (Dsb) proteins of Ehrlichia chaffeensis and Ehrlichia canis were identified which re

57 e proteins have been identified in Ehrlichia chaffeensis and Ehrlichia canis, including three molecul

58 onserved in the omp1 gene locus of Ehrlichia chaffeensis and p30 gene locus of E. canis despite marke

59 serologic cross-reactivity between Ehrlichia chaffeensis and the agent of HGE.

60 N-terminal TR region (18 amino acids) in E. chaffeensis and the complete TR (24 amino acids) in E. c

61 Western immunoblotting using purified E. chaffeensis and the HGE agent as antigens suggested that

62 ompare the genome sequences of strains of E. chaffeensis and to examine the virulence potentials of t

63 ocation of bacterially encoded protein by E. chaffeensis and to identify a specific binding motif and

64 eins in Anaplasma phagocytophilum, Ehrlichia chaffeensis, and Ehrlichia canis.

65 tease HtrA was detected on the surface of E. chaffeensis, and TRP120 was degraded by treatment of E.

66 Differential transmigration of E. chaffeensis- and A. phagocytophilum-infected leukocytes

67 Ehrlichia chaffeensis AnkA was recently reported to be translocate

68 er membrane proteins (P28 OMPs) of Ehrlichia chaffeensis are encoded by a multigene family.

69 Ehrlichia canis and E. chaffeensis are tick-borne obligatory intramonocytic ehr

70 recombinant P43 (rP43) did not react with E. chaffeensis as detected by indirect fluorescent antibody

71 rect immunofluorescence assay with Ehrlichia chaffeensis as the antigenic substrate.

72 First, E. chaffeensis avoided stimulation of or repressed the tran

73 E. chaffeensis binding to and subsequent infection of monoc

74 A type IV secretion effector of E. chaffeensis blocks mitochondrion-mediated host cell apop

75 E. chaffeensis BolA bound to the promoters of genes encodin

76 E. chaffeensis bolA complemented a stress-sensitive E. coli

77 During the intracellular development of E. chaffeensis, both P28 and OMP-1F were expressed mostly i

78 y of the mmpA gene in E. canis and Ehrlichia chaffeensis but not in the human granulocytic ehrlichios

79 e reticulate forms of E. canis and Ehrlichia chaffeensis but was notably found on extracellular morul

80 Therefore, we tested the hypothesis that E. chaffeensis can infect adult Drosophila melanogaster.

81 The heat-sensitive component of viable E. chaffeensis cells was essential for these signaling even

82 We assessed the E. chaffeensis clearance from the peritoneum, spleen, and l

83 mice infected with the tick cell-derived E. chaffeensis compared to DH82-grown bacteria.

84 Ehrlichia chaffeensis components that induce inflammation and the

85 se five proteins were similar in isolated E. chaffeensis-containing vacuoles and vacuole-free E. chaf

86 d, which was strikingly more prevalent in E. chaffeensis-containing vacuoles than in vacuole-free E.

87 E. chaffeensis CtrA bound to the promoters of late-stage tr

88 Our results suggest that E. chaffeensis CtrA plays a role in co-ordinating developme

89 to be surface exposed), were detected in E. chaffeensis cultured in human monocytic leukemia THP-1 c

90 tein-processing enzymes were expressed by E. chaffeensis cultured in the human promyelocytic leukemia

91 e, suggesting that it is not expressed by E. chaffeensis cultured in THP-1 cells.

92 rongly with TRP120 in HeLa cells and with E. chaffeensis dense-cored morulae and areas adjacent to mo

93 se data suggest that the host response to E. chaffeensis depends on the source of the bacteria and th

94 late nutrient uptake during intracellular E. chaffeensis development at both temperatures.

95 The P43 gene was not detected in E. chaffeensis DNA by Southern blot, and antisera against r

96 aplasma phagocytophilum, Ehrlichia canis, E. chaffeensis, E. ewingii, Rickettsia rickettsii, R. conor

97 duce PCR products with DNA extracted from E. chaffeensis-, E. canis-, or E. phagocytophila-infected s

98 When the three human isolates of E. chaffeensis, each belonging to a different genogroup, ar

99 In this study, we determined that the E. chaffeensis effector TRP120 is posttranslationally modif

100 With the recent discoveries of Ehrlichia chaffeensis, Ehrlichia ewingii, and "Borrelia lonestari,

101 smitted infectious agents, such as Ehrlichia chaffeensis, Ehrlichia ewingll, the Ehrlichia phagocytop

102 horetic mobility shift assays revealed an E. chaffeensis-encoded protein that specifically bound to t

103 e obligate intracellular bacterium Ehrlichia chaffeensis, even when administered well after infection

104 E. chaffeensis experiences temperature changes during trans

105 These novel findings reveal that E. chaffeensis exploits canonical and noncanonical Wnt path

106 survive and replicate within phagocytes, E. chaffeensis exploits the host cell by modulating a numbe

107 st and the most comprehensive analysis of E. chaffeensis-expressed proteins.

108 E. chaffeensis expresses a sensor kinase, PleC, and a cogna

109 the first to examine genetic variation in E. chaffeensis from a nonhuman vertebrate host.

110 was downregulated prior to the release of E. chaffeensis from host THP-1 cells and was upregulated at

111 de variation than previously reported for E. chaffeensis from infected humans or ticks.

112 rum titers, and 3) in vitro cultivation of E chaffeensis from peripheral blood.

113 of tandem repeats, were characterized for E. chaffeensis from white-tailed deer (Odocoileus virginian

114 ngly different among the three strains of E. chaffeensis: gamma interferon, CCL5, CXCL1, CXCL2, CXCL7

115 In all, 278 genes of the E. chaffeensis genome were verified as functional genes.

116 Bioinfomatic analysis of the E. chaffeensis genome, however, predicted genes encoding 15

117 one-fourth of all predicted genes of the E. chaffeensis genome, validating that they are functionall

118 e deletion and insertion mutations in the E. chaffeensis genome.

119 us transcription across the entire Ehrlichia chaffeensis genome.

120 28- and a 27-kb locus in the E. canis and E. chaffeensis genomes, respectively.

121 nses to another outer membrane protein of E. chaffeensis (GP120) showed similar temporal and quantita

122 E. canis gp36 and E. chaffeensis gp47 were differentially expressed only on t

123 ptide repeat units from E. canis gp36 and E. chaffeensis gp47 were substantially less immunoreactive

124 We recently reported that E. chaffeensis grown in tick cells expresses different prot

125 rmissive and nonpermissive conditions for E. chaffeensis growth.

126 E. chaffeensis has a multigene family of major outer membra

127 articular, despite its small genome size, E. chaffeensis has four tandem virB6 paralogs (virB6-1, -2,

128 ely related A. phagocytophilum and Ehrlichia chaffeensis have been shown to localize to the host cell

129 Whereas Ehrlichia chaffeensis (HME) often causes meningoencephalitis, this

130 atment of E. chaffeensis with recombinant E. chaffeensis HtrA.

131 All 16 E. chaffeensis IFA-positive sera reacted with rP30.

132 rditis and multiorgan failure from Ehrlichia chaffeensis in a previously healthy adolescent is descri

133 muris strain that are closely related to E. chaffeensis in C57BL/6 mice.

134 This is the first demonstration of RNA of E. chaffeensis in infected blood and acquisition-fed male,

135 Entry and proliferation of E. chaffeensis in THP-1 cells were significantly blocked by

136 irB6 proteins and VirB9 were expressed by E. chaffeensis in THP-1 cells, and amounts of these five pr

137 Only one paralog was transcribed by E. chaffeensis in three developmental stages of Amblyomma a

138 Multiple lipoproteins expressed by E. chaffeensis in vitro and in vivo may play key roles in p

139 ial in preventing lysosomal fusion of the E. chaffeensis inclusion compartment.

140 treatment induced lysosomal fusion of the E. chaffeensis inclusion in a human monocytic leukemia cell

141 E. chaffeensis induced rapid tyrosine phosphorylation of PL

142 Although E. chaffeensis induces the generation of several cytokines

143 strongly with antibodies in sera from an E. chaffeensis-infected dog and human monocytotropic ehrlic

144 ofluorescent microscopy in the nucleus of E. chaffeensis-infected host cells and was detected in nucl

145 More E. chaffeensis-infected monocytes transmigrated than uninfe

146 Analyses of human sera showed that E. chaffeensis-infected patients also generated serological

147 from E. canis-infected dogs but not from E. chaffeensis-infected patients.

148 E. chaffeensis infection activated the phosphatidylinositol

149 hibitor, globomycin, was found to inhibit E. chaffeensis infection and lipoprotein processing in HL-6

150 1, which were strongly upregulated during E. chaffeensis infection and were also upregulated by direc

151 Diagnosis of E. chaffeensis infection by indirect immunofluorescence ass

152 E. chaffeensis infection did not result in dramatic changes

153 E. chaffeensis infection in a human monocyte cell line (THP

154 Previous studies of Ehrlichia chaffeensis infection in the mouse have demonstrated tha

155 ated the effects of c-di-GMP signaling on E. chaffeensis infection of the human monocytic cell line T

156 t susceptible SCID mice from fatal Ehrlichia chaffeensis infection, an observation that has been hypo

157 After E. chaffeensis infection, canonical and noncanonical Wnt si

158 knockouts, when challenged with a second E. chaffeensis infection.

159 ed molecular patterns and pathogenesis of E. chaffeensis infection.

160 macrophage-tropic bacterium, we assessed E. chaffeensis infections in three mouse strains with diffe

161 In the absence of MHC-II alleles, E. chaffeensis infections persisted throughout the entire 3

162 tyldimethysilyl)-c-di-GMP (CDGA) inhibits E. chaffeensis internalization into host cells by facilitat

163 Ehrlichia chaffeensis invades and survives in phagocytes by modula

164 Our results suggest that E. chaffeensis invasion is regulated by c-di-GMP signaling,

165 ocytotropic ehrlichiosis caused by Ehrlichia chaffeensis is a life-threatening, tick-borne, emerging

166 Ehrlichia chaffeensis is an obligate intracellular bacterium that

167 Ehrlichia chaffeensis is an obligate intracellular bacterium that

168 Ehrlichia chaffeensis is an obligate intracellular bacterium that

169 Ehrlichia chaffeensis is an obligate, intracellular bacterium, tra

170 Ehrlichia chaffeensis is an obligately intracellular bacterium tha

171 Ehrlichia chaffeensis is an obligately intracellular bacterium tha

172 Ehrlichia chaffeensis is an obligately intracellular bacterium tha

173 Ehrlichia chaffeensis is an obligately intracellular bacterium tha

174 Ehrlichia chaffeensis is an obligately intracellular gram-negative

175 Ehrlichia chaffeensis is an obligately intracellular Gram-negative

176 Ehrlichia chaffeensis is an obligatory intracellular and cholester

177 Ehrlichia chaffeensis is an obligatory intracellular bacterium of

178 Ehrlichia chaffeensis is an obligatory intracellular bacterium whi

179 We previously demonstrated that E. chaffeensis is capable of growing in Drosophila S2 cells

180 The data indicate that E. chaffeensis is exposed to the extracellular milieu durin

181 n this study suggest that the membrane of E. chaffeensis is very complex, having many expressed prote

182 obligatory intracellular pathogen, Ehrlichia chaffeensis, is characterized by formation of bacterial

183 an ehrlichial organism closely related to E. chaffeensis isolated from Ixodes ovatus ticks in Japan,

184 Although E. chaffeensis lacks entire lipopolysaccharide and most pep

185 l cytokines and chemokines by leukocytes, E. chaffeensis lacks lipopolysaccharide and peptidoglycan.

186 To study in vivo E. chaffeensis lipoprotein expression and host immune respo

187 ponses to E. chaffeensis lipoproteins, 13 E. chaffeensis lipoprotein genes were cloned into a mammali

188 n expression and host immune responses to E. chaffeensis lipoproteins, 13 E. chaffeensis lipoprotein

189 otein with < or =69.1% identity to P28 of E. chaffeensis, < or =67.3% identity to P30 of E. canis, an

190 Removal of PBP from E. chaffeensis lysate using penicillin affinity column and

191 Western blot analysis of E. canis and E. chaffeensis lysates with the anti-rMmpA serum resulted i

192 Both protective Abs recognized the E. chaffeensis major outer membrane protein (OMP)-1g.

193 When E. chaffeensis matures into an infectious form, morulae bec

194 Western blot analysis of E. chaffeensis membrane and soluble fractions using antibod

195 To investigate the surface proteins of E. chaffeensis, membrane-impermeable, cleavable Sulfo-NHS-S

196 n TRP32 and host targets localized to the E. chaffeensis morulae or in the host cell cytoplasm adjace

197 study, we assessed two clonally purified E. chaffeensis mutants with insertions within the genes Ech

198 [(32)P]c-di-GMP bound several E. chaffeensis native proteins and two E. chaffeensis recom

199 and was located downstream of two Ehrlichia chaffeensis omp-1 homologs and a decarboxylase gene (ubi

200 ptides are conserved between E. muris and E. chaffeensis OMP-19, and they elicited IFN-gamma producti

201 if the ELISA is positive), we identified E. chaffeensis or a serologically and antigenically similar

202 ta or Wisconsin were found not to be from E. chaffeensis or E. ewingii and instead to be caused by a

203 lar to those of human infection by Ehrlichia chaffeensis or EMLA.

204 l, membrane, and immunogenic proteomes of E. chaffeensis originating from macrophage and tick cell cu

205 different between mice infected with the E. chaffeensis originating from tick cells or macrophages.

206 Here we demonstrate that isolated E. chaffeensis outer membranes have porin activities, as de

207 P) with DNA sequencing revealed an Ehrlichia chaffeensis p200 interaction located within host promote

208 Homologous sequences for Ehrlichia chaffeensis p28 were compared to sequences of primers de

209 plasma phagocytophilum MSP2 (p44), Ehrlichia chaffeensis p28-OMP, Ehrlichia canis p30, and Ehrlichia

210 The cytokine-inducing activity by E. chaffeensis PBP provides novel insights into pathogen-as

211 Recombinant E. chaffeensis PleD showed diguanylate cyclase activity as

212 e white-tailed deer agent, and additional E. chaffeensis-positive samples).

213 The surface proteins of Ehrlichia chaffeensis provide an important interface for pathogen-

214 al E. chaffeensis native proteins and two E. chaffeensis recombinant I-site proteins, and this bindin

215 e sensitive than the PCR for detection of E. chaffeensis regardless of the nature of the specimens.

216 ule inhibitor had a significant impact on E. chaffeensis replication and recruitment of the TRP120-in

217 cleotide salvage pathway is essential for E. chaffeensis replication and that it may be important for

218 on of the uridine-cytidine kinase affects E. chaffeensis replication in human macrophages.

219 e obligate intracellular bacterium Ehrlichia chaffeensis resides in early endosome-like vacuoles and

220 ns from macrophage- and tick cell-derived E. chaffeensis, respectively.

221 Addition of E. chaffeensis resulted in rapid increases in the level of

222 chia muris, a pathogen closely related to E. chaffeensis, resulted in anemia, thrombocytopenia, and a

223 use of template DNA extracted from Ehrlichia chaffeensis, Rickettsia rickettsii, and Bartonella hense

224 ae) two-hybrid analysis demonstrated that E. chaffeensis-secreted tandem repeat protein 120 (TRP120)

225 Ehrlichia chaffeensis secretes tandem repeat protein (TRP) effecto

226 es, identity of the primers to homologous E. chaffeensis sequences, and the availability of similarly

227 he members of the p28 multigene family of E. chaffeensis, sera from two beagle dogs experimentally in

228 n leukemia cell line THP-1 was exposed to E. chaffeensis, significant upregulation of IL-8, IL-1beta,

229 E. chaffeensis-specific cytotoxic T cells were not detected

230 The E. chaffeensis-specific immunoglobulin G response was consi

231 S6-Cd4(tm1Knw) mice also developed active E. chaffeensis-specific immunoglobulin G responses that wer

232 acrophage activation and the synthesis of E. chaffeensis-specific Th1-type immunoglobulin G response.

233 ay of 147,027 chromosome positions of the E. chaffeensis strain Arkansas genome.

234 The E. chaffeensis strain Wakulla induces diffuse hepatitis wit

235 equence polymorphisms in several genes in E. chaffeensis strains have been reported, global genomic d

236 E. chaffeensis strains induce strikingly variable inflammat

237 f host cytokine and chemokine profiles by E. chaffeensis strains underlies the distinct host liver di

238 Genomic DNA was extracted from purified E. chaffeensis strains Wakulla and Liberty, and comparative

239 When the E. chaffeensis strains were inoculated into severe combined

240 revealed distinct virulence phenotypes of E. chaffeensis strains with defined genome sequences.

241 responses and the in vivo replication of E. chaffeensis suggests that D. melanogaster is a suitable

242 E. coli expressing E. chaffeensis SurE exhibited increased resistance to osmot

243 proteins provides novel insights into the E. chaffeensis surface and lays the foundation for rational

244 Additional E. chaffeensis surface proteins detected were OMP85, hypoth

245 The identification of E. chaffeensis surface-exposed proteins provides novel insi

246 The E. chaffeensis T1S effector TRP120 is conjugated to SUMO at

247 Recently we have determined that E. chaffeensis tandem repeat proteins (TRPs) are type 1 sec

248 Recently, molecular interactions of E. chaffeensis tandem repeat proteins 47 and 120 (TRP47 and

249 hat documents that insertion mutations in E. chaffeensis that cause attenuated growth confer protecti

250 ve proteins of Ehrlichia canis and Ehrlichia chaffeensis that have been characterized include a famil

251 tick-borne zoonoses, is caused by Ehrlichia chaffeensis that lacks endotoxin and peptidoglycan.

252 ive dogs, or when dogs were infected with E. chaffeensis, the animals developed delayed-type hypersen

253 Infection of humans with Ehrlichia chaffeensis, the etiologic agent of human monocytic ehrl

254 Ehrlichia chaffeensis, the etiologic agent of human monocytic ehrl

255 Ehrlichia chaffeensis, the etiologic agent of human monocytic ehrl

256 sma (Ehrlichia) phagocytophila and Ehrlichia chaffeensis, the etiologic agents of granulocytic and mo

257 t amplify the 200-bp target amplicon from E. chaffeensis, the human granulocytic ehrlichiosis agent,

258 found in an intravacuolar pathogen Ehrlichia chaffeensis, the tick-borne causative agent of human mon

259 two surface-expressed antigens of Ehrlichia chaffeensis, the variable-length PCR target (VLPT) and t

260 In the rickettsial pathogen Ehrlichia chaffeensis, the virBD genes are split into two operons,

261 E. chaffeensis, therefore, can recruit interacting signal-t

262 ndocytosis directs A. phagocytophilum and E. chaffeensis to an intracellular compartment secluded fro

263 d and compared in 12 clinical isolates of E. chaffeensis to determine allele variation.

264 mblyomma americanum ticks before or after E. chaffeensis transmission to naive dogs.

265 anonical and noncanonical Wnt pathways by E. chaffeensis TRP effectors stimulates phagocytosis and pr

266 r closely related interacting partners of E. chaffeensis TRP32, TRP47, and TRP120 demonstrate a molec

267 E. chaffeensis TRP75 and E. canis TRP95 were immunoprecipit

268 c C terminus of E. canis TRP95 but not in E. chaffeensis TRP75.

269 two-hybrid analysis demonstrated that an E. chaffeensis type 1 secretion system substrate, TRP32, in

270 The current study identified a novel E. chaffeensis ubiquitin ligase and revealed an important r

271 Second, E. chaffeensis up-regulated NF-kappaB and apoptosis inhibit

272 rly Ehrlichia) phagocytophilum and Ehrlichia chaffeensis, upon infection of humans, replicate in host

273 We recently reported that E. chaffeensis utilizes a type 1 secretion (T1S) system to

274 lycoprotein (gp19) ortholog of the Ehrlichia chaffeensis variable-length PCR target (VLPT) protein.

275 The results suggest that E. chaffeensis VirB9, the quadruple VirB6 proteins, and the

276 This is the most extensive study of E. chaffeensis VLPT and 120-kDa antigen gene genetic variat

277 l-terminal amino acid homology (59%) with E. chaffeensis VLPT and the same chromosomal location; howe

278 e same chromosomal location; however, the E. chaffeensis VLPT gene (594 bp) has tandem repeats that a

279 These results indicate that the E. chaffeensis Wakulla strain can induce inflammatory respo

280 on of penicillin-binding protein (PBP) in E. chaffeensis was analyzed by reverse-transcription polyme

281 Macrophage-grown E. chaffeensis was cleared in 2 weeks from the peritoneum,

282 ocytotropic ehrlichiosis caused by Ehrlichia chaffeensis was reported in 1987.

283 E. chaffeensis was sensitive to closantel, an HK inhibitor.

284 Ehrlichia chaffeensis was suspected as the causal agent but was no

285 Expression of PBP by E. chaffeensis was upregulated during its intracellular lif

286 In E. chaffeensis we predicted three pairs of putative two-com

287 ria and that of macrophages infected with E. chaffeensis, we have identified few genes that are commo

288 ter membrane protein gene (p28) of Ehrlichia chaffeensis were analyzed to determine the mechanism of

289 and virB6) of both A. phagocytophila and E. chaffeensis were arranged downstream from a sodB gene an

290 Targeted mutations in E. chaffeensis were created to disrupt two genes, and also

291 Antibodies reactive with E. chaffeensis were detected in 14 (67%) of the 21 PCR-posi

292 beagle dogs experimentally infected with E. chaffeensis were evaluated for the presence of specific

293 hromatography purified, and native PBP of E. chaffeensis were investigated for their ability to induc

294 five genes in both A. phagocytophila and E. chaffeensis were polycistronically transcribed and contr

295 ominant outer membrane proteins of Ehrlichia chaffeensis were transcribed in blood monocytes of dogs

296 s, this RT-PCR is useful for detection of E. chaffeensis when a high sensitivity is required.

297 Although pretreatment of E. chaffeensis with CDGA did not reduce bacterial binding t

298 , and TRP120 was degraded by treatment of E. chaffeensis with recombinant E. chaffeensis HtrA.

299 ng regulates aggregation and sessility of E. chaffeensis within the inclusion through stabilization o

300 hypothesis that immune responses against E. chaffeensis would be different if the mice are challenge