ライフサイエンスコーパス: channel current

1 ts NCLX activity, and impairs SOCE and Orai1 channel current.

2 ormation that can lead to inhibition of GLIC channel current.

3 e channel sufficient to account for the open channel current.

4 or NADP(+), induces a large increase in open channel current.

5 tion in cardiac repolarizing I(Ks) potassium channel current.

6 an increase in the size of the atypical Ca2+ channel current.

7 pen probability without modifying the single channel current.

8 h we measure as a change in the source/drain channel current.

9 is was not accompanied by a change in Ca(2+) channel current.

10 nvestigating the effect of TNFalpha on TASK3 channel current.

11 produced a substantial increase in potassium channel current.

12 Xenopus laevis oocytes elicited S-type anion channel currents.

13 ) release induced by increased L-type Ca(2+) channel currents.

14 activation kinetics of baclofen-induced GIRK channel currents.

15 balance in favor of D(s) and increasing ion channel currents.

16 ns, and the GBP-induced reduction in calcium channel currents.

17 pendent activation of inwardly rectifying K+ channel currents.

18 ical because of the rapid rundown of calcium channel currents.

19 ase C deplete PIP(2) and reduce those Ca(2+) channel currents.

20 n yet together synergize to inhibit Ca(v)3.2 channel currents.

21 ity and increased constitutive and activated channel currents.

22 tion, nor did it affect voltage-activated Ca channel currents.

23 is lost, to make in situ recordings of nAChR channel currents.

24 either Orai1 or Orai3 markedly inhibits ARC channel currents.

25 ression of Orai1 increases both CRAC and ARC channel currents.

26 by the recording of maximally activated CRAC channel currents.

27 pparent reduction of the amplitude of single-channel currents.

28 antibodies induced the appearance of single-channel currents.

29 hannel-like structures and elicit single ion-channel currents.

30 Likewise, GsMTx4 decreases gA single-channel currents.

31 d G551D-CFTR, DeltaF508-CFTR, and 8SA mutant channel currents.

32 e individual showed markedly decreased NALCN channel currents.

33 hat has precluded direct recording of single-channel currents.

34 f hMSC families with distinct functional ion channel currents.

35 tor of high-voltage-activated N-type calcium channel currents.

36 in parallel with increased potassium (K(+)) channel currents.

37 f L-type channel currents compared to N-type channel currents.

38 uced calcium permeability and reduced single-channel currents.

39 ion (LoF) point changes leading to decreased channel currents.

40 e predictive of whether a ligand could evoke channel currents.

41 w that Delta27-264 CFTR can restore chloride channel currents.

42 ted abnormal SCN5A mRNA splicing reduced Na+ channel current 91.1+/-9.3% to a range known to cause su

43 in the model by inhibiting appropriate ionic channel currents according to experimentally reported co

44 individual experiment (i.e., measurement of channel currents across the endolysosomal membrane), inc

45 ed opening frequency and open time of single channel currents activated by maximally effective concen

46 We describe GluA3 single channel currents activated by nitrowillardiine (NO(2)W)

47 ficantly decreased Ca(2+)-dependent chloride channel currents activated in response to the nanoporati

48 d alpha-conotoxins Vc1.1 and RgIA on calcium channel currents after transient knockdown of the GABA(B

49 in a substantial enhancement of the calcium channel currents, albeit less than that produced by wild

50 leotides) results in a large increase in ion channel current, allowing accurate quantitation of the k

51 a toxin ProTx-II, which also inhibits sodium channel currents, altered the gating-pore currents in mu

52 -->O isomerization process; and 3), the open-channel current amplitude (i(0)), which reports whether

53 e or by homoquinolinate has identical single-channel current amplitude and mean open-channel duration

54 ased ENaC open probability (P(o)) and single-channel current amplitude but not the unit conductance.

55 With the reported method, single-channel current amplitude of native voltage-gated calciu

56 of -20 mV, we found that the average single-channel current amplitude was approximately 0.04 pA, inc

57 ied by a significant reduction of the single-channel current amplitude, indicating an interaction of

58 ide-out patches, but has no effect on single-channel current amplitude.

59 that GLT-1c and EAAT5 also differ in single-channel current amplitudes of associated anion channels.

60 tivation by combining mutagenesis and single-channel current analyses.

61 es were also functionally assessed by single channel current analysis.

62 function of Shank proteins is to regulate L-channel current and activity induced gene expression.

63 ased and decreased shn-1 gene dosage alter L-channel current and activity-induced expression of a CRH

64 a reduction in NPo of a 238 pS arterial KCa channel current and an increase in [Ca(2+)]i level in fr

65 tates in both cycles are responsible for the channel current and fit the current transients under the

66 It has been shown that the channel current and the conformational flexibility of co

67 tions and EAAT2/EAAT4 measurements of single-channel currents and anion/cation selectivities.

68 f Panx1 at Cys-40 and Cys-346 inhibits Panx1 channel currents and ATP release.

69 inic acetylcholine receptors can enhance ion channel currents and downstream effects of alpha7 stimul

70 We recorded single-channel currents and estimated the gating rate and equil

71 ished both oxyhb-induced suppression of K(v) channel currents and oxyhb-induced constriction of isola

72 s with RGS9 constructs enhanced basal Ca(2+) channel currents and reduced D(2) dopamine receptor modu

73 vel differential regulation of L-type Ca(2+) channel currents and SR load by GRK2.

74 sted that hyperpolarization-activated cation channel currents and T-type calcium channel currents may

75 bunits results in a graded reduction in CRAC channel currents and that this effect is independent of

76 pool in tsA-201 cells, we monitored KCNQ2/3 channel currents and translocation of PHPLCdelta1 domain

77 d G-protein-coupled inwardly rectifying K(+) channel current, and attenuated calcium current, and ind

78 crease in alpha1C expression, enhanced Ca(L) channel current, and elevated Ca2+-dependent tone.

79 These results suggested a prominent Na+ channel current, and therefore we patch-clamped isolated

80 esult of reciprocal actions on VRAC and KATP channel currents, and could contribute towards the stimu

81 s exhibit increased basal and GPCR-activated channel currents, and increased Ca(2+) fluorescence resp

82 pits, nearly complete inhibition of KCNQ2/3 channel currents, and loss of membrane ruffling.

83 ole-cell patch clamp was used to assess K(+) channel currents, and mRNA and protein expression was de

84 asing G-protein-gated inwardly rectifying K+ channel currents, and presynaptically inhibiting release

85 t the axon initial segment, attenuates Na(v) channel currents, and reduces the excitability of hippoc

86 and characterize their whole-cell and single-channel currents, and surface and total levels.

87 ressing TRPM8, elicited Ca(2+) responses and channel currents, and those were inhibited in the presen

88 GIRK (G-protein-gated inwardly rectifying K+ channel) current, and attenuated calcium current, and in

89 d-rectifying K+ channels and background (Cl- channel) current, and to a parallel loss in sensitivity

90 y was carried out to determine whether these channel currents are involved in the PGE(2)-induced sens

91 expression of Orai3 alone has no effect, ARC channel currents are specifically increased by expressio

92 The measured transmembrane single channel currents are very high, rivaling those typically

93 creased their ATP-sensitive K+ channel (KATP channel) currents as extracellular glucose levels increa

94 lts from loss of a direct effect on the Ca2+ channel current, as shown in a transfected cell line dev

95 s and autocorrelation coefficients of single-channel currents, as well as by the predicted time cours

96 ealed a similar density of whole-cell Ca(2+) channel current between single smooth muscle cells (SMCs

97 sensitive (TTX-S) and -resistant (TTX-R) Na+ channel currents between isolectin B4 (IB4)-positive and

98 dden Markov Models (MHMMs) to the problem of channel current blockade clustering and associated analy

99 i1 alone determine the magnitude of the CRAC channel currents, both Orai1 and the closely related Ora

100 GsMTx4 decreases inward SAC single-channel currents but has no effect on outward currents,

101 the patch pipette solution blocked TRPC1/C5 channel currents but potentiated by about six-fold TRPC6

102 NADPH inhibits inactivation and potentiates channel current, but the mechanism behind this effect is

103 illustrated by the suppression of KCNQ K(+) channel current by activation of M(1) muscarinic recepto

104 Transient, ~95% blockage of the channel current by alpha-syn was observed when 1), alpha

105 EGTA reduced PC2hst channel currents by 86%, with a t1/2 = 3.6 min, whereas

106 atase (VSP) suppresses Ca(V)1.2 and Ca(V)1.3 channel currents by approximately 35% and Ca(V)2.1 and C

107 The blockage of L-type voltage-gated Ca(2+) channel currents by glacontryphan-M requires calcium bin

108 te, all mutations increase whole-cell K(ATP) channel currents by reducing channel inhibition by MgATP

109 t of silencing and permanent blockade of the channel current cannot necessarily be attributed to the

110 -activated cyclic nucleotide-gated (HCN) ion channel current characteristics.

111 m channels show a marked reduction of L-type channel currents compared to N-type channel currents.

112 conductance calcium-activated potassium (BK) channel currents contribute to action potential (AP) rep

113 The D540C-L666C channel current decreased in an oxidizing environment in

114 Whole cell K(ATP) channel current density (activated by metabolic inhibiti

115 s because of better-maintained L-type Ca(2+) channel current density and no increase in sodium-Ca(2+)

116 teroid hormone-induced up-regulation of BKCa channel current density and NS1619-mediated relaxations,

117 KCa channel beta1 subunit abundance and BKCa channel current density in uterine arteries from pregnan

118 howed that R222Q Nav1.5 did not alter sodium channel current density, but did left shift steady-state

119 sheep, hypoxia significantly inhibited BKCa channel current density, decreased NS1619-induced relaxa

120 We used real-time monitoring of K(ATP) channel current density, immunohistochemistry, and bioti

121 tein caused a significant increase in Ca(2+) channel current density, whereas lentivirus-mediated CRM

122 (v)1.5 membrane targeting, and reduced Na(+) channel current density.

123 lta-1(-/-) DRG neurons exhibit lower calcium channel current density.

124 duration but significantly depressed sodium channel current-dependent parameters causing a reduction

125 oving boundary electrophoresis (GEMBE), with channel current detection.

126 y of these compounds in potentiating the SK2 channel current determined by electrophysiological recor

127 Voltage-gated Ca(2+) channel currents determined by patch-clamp recording wer

128 sed in HEK-293 cells, the kinetics of single-channel currents differ only slightly, but they are subs

129 The ion channel current directly charges the quantum capacitance

130 CRAC-/STIM1-mediated Orai channel currents display characteristic hallmarks such a

131 t pharmacologic enhancement of mutated TASK3 channel current during development may, therefore, provi

132 triphosphate (GTPgammaS) binding and GIRK1/4 channel current effects in Xenopus oocytes where the mut

133 ell-attached patch-clamp recording of single-channel currents elicited by 30-10000 microM glycine.

134 ted with decreased cardiac voltage-gated Na+ channel current (encoded by SCN5A), and the changes have

135 her depolarized, then the hyperpolarizing BK channel current exceeds its facilitatory effect, causing

136 wentoxin-IV (HWTX-IV), which inhibits sodium channel current, exclusively enhanced inward gating-pore

137 RTX-induced single channel currents exhibit multiple conductance states and

138 easure the activity of BK and GirK potassium channel currents expressed in Xenopus laevis oocytes.

139 Single-channel currents for formate, hypophosphite and nitrite

140 his gating isomerization, we examined single-channel currents from adult mouse muscle AChRs that isom

141 eptor function by PbTx-2, we recorded single-channel currents from cell-attached patches.

142 ological evidence for the presence of single channel currents from isolated primary cilia of LLC-PK1

143 l signals, we recorded whole-cell and single-channel currents from mouse hair cells that expressed Tm

144 We studied single-channel currents from neuromuscular acetylcholine recept

145 ments to receptor gating, we recorded single-channel currents from outside-out patches containing a s

146 ring postnatal development, we recorded Ca2+ channel currents from Purkinje cells in cerebellar slice

147 Here we compare agonist-elicited single channel currents from receptors assembled with an excess

148 smitter binding and channel gating in single-channel currents from recombinant NR1/NR2A receptors, th

149 ntially distinguishing effects requiring ion channel currents from signaling induced by nonconducting

150 mall-conductance calcium-activated potassium channel currents generate an apamin-sensitive medium AHP

151 Studying ion channel currents generated distally from the recording s

152 In TRPC1(-/-) VSMCs, CPA-induced channel currents had 3 subconductance states of 14, 32,

153 Single-channel currents had a unitary conductance of 210 pS, ty

154 yrate (PDBu) in inside-out patches and these channel currents had a unitary conductance of about 2 pS

155 tivated cyclic nucleotide-gated cation (HCN) channel currents have been identified in various periphe

156 f the N-terminal EF hand transforms the CRAC channel current (I(CRAC)) into a constitutively active,

157 used to evaluate inactivation of peak sodium channel current (I(Na)) in myocytes isolated from canine

158 inactivation, and because facilitation of Ca-channel currents (I(Ca)) masks the extent and kinetics o

159 t it did inhibit Ca(V)2 voltage-gated Ca(2+) channel currents (I(Ca)).

160 bath application of 10 nm ET-1 evoked cation channel currents (I(cat)) with subconductance states of

161 tions result in the potent inhibition of Nav channel currents (I(Na)) and in changes in the voltage d

162 There was no change in L-type Ca2+ channel current (ICa) density but the ability of ICa to

163 er IL-6 reduced Ca2+ influx via L-type Ca2+ -channel current (ICa,L).

164 llodynia by modulating voltage-gated calcium channel currents (ICa(V)).

165 overexpression did not affect L-type calcium channel current (ICaL) under basal conditions with ICaL

166 ta sets on AF-induced changes of major ionic channel currents (ICaL, IKur, Ito, IK1, IKs, INaCa) and

167 EA induced cation channel current (Icat) in SW982 cells with biophysical c

168 entrations of Ang II (1 nM) activated cation channel currents (Icat1) with conductances states of abo

169 Painful axotomy decreases K(ATP) channel current (IK(ATP)) in primary afferent neurons.

170 s but no significant changes in transient K+-channel current (IKA) amplitude.

171 conductance, we measured Shaker-P475D single-channel current in a wide range of symmetrical K(+) conc

172 e produced greater inhibition of peak sodium channel current in atrial cells isolated from HF versus

173 Here, we recorded the voltage-gated calcium channel current in nucleated patches from layer 5 pyrami

174 ane stretch suppresses an XE991-sensitive Kv channel current in patch-clamped vascular smooth muscle

175 properties consistent with an epithelial Na+ channel current in some cells, and a calcium ion-insensi

176 described by the model: the ratio of single channel current in the presence of PEG to that in the po

177 e physiological stimulus that enhances Ca(L) channel current in the vascular smooth muscle cells (VSM

178 The amplitudes of their single-channel currents in a physiological concentration of ext

179 potently inhibits the Abeta-induced calcium channel currents in artificial lipid membranes.

180 wever, mAb 131 did not reduce fetal AChR ion channel currents in electrophysiological experiments.

181 DQP-1105 inhibited single-channel currents in excised outside-out patches without

182 nase inhibitor alloxan increases KATP single-channel currents in glucose-excited neurons in a manner

183 By action spectroscopy of photoinduced channel currents in HEK293 cells and absorption spectros

184 cordings of both macroscopic and single dSlo channel currents in identified neurosecretory neurons in

185 in ex vivo slices significantly reduced KCa2 channel currents in layer V IL-PFC pyramidal neurons, co

186 tein-activated inwardly rectifying potassium channel currents in mouse brain slices containing locus

187 namic interaction among Kir2, Kleak, and HCN channel currents in shaping membrane potential and the t

188 effects of a methanethiosulfonate reagent on channel currents in single and double Cys mutants, we id

189 l tools to dissect the various kinds of K(+) channel currents in situ.

190 i and a beta-subunit antibody) abolished Kv7 channel currents in the absence of either Gbetagamma sub

191 nd CaV beta 2a transfected cells elicit Ca2+ channel currents in the absence of these small G protein

192 GABARs in HEK 293 cells and recorded single channel currents in the cell-attached configuration usin

193 cules to examine their ability to induce ion channel currents in the whole spermatozoa.

194 KCNQ channel currents in these cells were potently reduced by

195 also ablated the effects of hypoxia on BKCa channel currents in uterine arteries.

196 sible hidden Markov model for the underlying channel current, in which successive measurements of the

197 ncreases linearly as the amplitude of single channel currents increases, while the open time for inwa

198 f the membrane to differentially enhance ERG channel currents independent of channel state.

199 By comparing whole-cell and single channel currents induced by the agonists, we provide a m

200 We hypothesized that Cav3.1 T-type Ca2+ channel currents inhibit thalamic sensory transmission t

201 and tissue levels, we now recognize that ion channel current is the sum of biophysical (gating, perme

202 Electrophysiological recording of single-channel currents is the most direct method available for

203 ChRs tested the Lys-132 homolog also alters channel current kinetics.

204 ed VZV isolates induce changes in sodium ion channel currents known to be associated with neuropathic

205 Calcium influx activates the BK channel current, leading to potassium efflux that increa

206 At the single-channel current level, ATP-dependent gating transitions

207 d cation channel currents and T-type calcium channel currents may account for some of the membrane pr

208 similar mutation-induced alterations in IKs channel currents may be caused by different molecular me

209 ta2gamma2L and alpha6(R46W)beta2delta single-channel current mean open times and burst durations were

210 Thus single channel current measurements reveal bases for the distin

211 Single-channel current measurements show that the channel funct

212 ted FETs, we have demonstrated that the high channel currents obtained at low gate voltages result fr

213 Simultaneous kinetic analysis of single-channel currents obtained at various GABA concentrations

214 method that allows measurement of the single-channel current of ion channels even when it is too smal

215 choline (ACh) binding and gating from single channel currents of fetal mouse AChRs expressed in tissu

216 A kinetic analysis of spontaneous channel currents of PC2 showed that four intrinsic, non-

217 Single-channel current openings were on average 32% shorter in

218 not due to changes in voltage-gated calcium channel currents or activation kinetics.

219 By dramatically suppressing channel currents, PIP3 may generate a shift in odorant s

220 e predictive of a ligand's ability to induce channel currents points to these conformational biosenso

221 old mice also exhibit smaller L-type calcium channel currents, providing a plausible mechanism that l

222 r voltage clamp, and the ensemble of the ion channel currents reconstructed in silico generates rhyth

223 e, consistent with the increased fast sodium channel current recorded from BACE1-null hippocampal neu

224 used kinetic analyses of equilibrium single-channel currents recorded from individual GluN1/GluN2A r

225 On the basis of single-channel currents recorded from the muscle nicotinic acet

226 By using single-channel current recording, we define discrete assembly i

227 Furthermore, we demonstrate using channel current recordings and binding assays that OAADP

228 Functional studies using single channel current recordings of the sensorless pore module

229 Further, from one-channel current recordings, we found that 4 mM NPA cause

230 e and alter the direction and amount of open channel current rectification.

231 pe Arg83 and variant Gly83 ClC-K(a) chloride channel currents revealed approximately 50% loss-of-func

232 y decreasing the calcium-activated potassium channel current (SK), as well as elevates dopaminergic n

233 e described the properties of store-operated channel currents (SOCs) in freshly dispersed rabbit port

234 We have measured single-channel currents, spontaneous gating, and rectification

235 ed Tmc1 and Tmc2 had a broad range of single-channel currents, suggesting multiple heteromeric assemb

236 cytes show hyperactivation of L-type calcium channel current that could not be reversed with palmitat

237 utward-directed current precedes the passive channel current that has the opposite direction at physi

238 K(+) channel current that normally mediates K(+) uptake for s

239 -1)) all caused a 20% increase in maxi-K(Ca) channel current that was blocked by AG-1478 or by knock-

240 ed large-conductance, Ca2+-sensitive K+ (BK) channel currents that could be activated by neuronal sti

241 aracterization of prolonged bursts of single-channel currents that occur with PNU-120596 provide a re

242 We estimated (from single-channel currents) the components of energy for gating ar

243 ous alpha5beta1 integrin ligand, enhances BK channel current through both Ca(2+)- and phosphorylation

244 Whole-cell K(ATP) channel currents through homomeric and heterozygous F35V

245 potentiation, we examined ACh-induced single-channel currents through the human neuronal alpha7 acety

246 presence of tRNA, blocking events of single-channel currents through the pore formed by recombinant

247 aveolae by MCD leads to a decrease in the BK channel current thus increasing cell excitability, and (

248 We use the average channel current to create a conductance image and the ra

249 ifies oscillatory dendritic Ca(2+) and Na(+) channel currents to lead to the initiation of high-frequ

250 PA1) into lipid bilayers and recorded single-channel currents to understand its inherent thermo- and

251 he requirement of calcium-activated chloride channels currents to mediate pheromone activation.

252 ucing plausible reaction schemes from single-channel current traces is time-consuming and difficult.

253 ed, which led to the proposal that gA single-channel current transitions result from closed-open tran

254 (anion) in Vicia faba guard cells, measuring channel current under a voltage clamp while manipulating

255 It is likely that these single channel currents underlie the noradrenaline-activated in

256 resolution structures of several prokaryotic channels, current understanding of their physiological r

257 ovalent modulation of macroscopic and single-channel currents using extracellularly and intracellular

258 um antibodies and their effects on potassium channel currents using human embryonic kidney cells expr

259 bition we recorded wild-type and mutant CFTR channel currents using inside-out membrane patches from

260 recorded wild-type and mutant CFTR chloride-channel currents using patch-clamp methods.

261 gests that mutant MEC-10 proteins may affect channel current via MEC-2.

262 Single channel current-voltage curves are computed over hundred

263 Single-channel current-voltage relationships were nonlinear in

264 outside-out patches showed that in all Kir2 channels current-voltage relationships display a 'crosso

265 In HCl solutions, the single-channel current was essentially invariant with changes i

266 We found that at P40-50, the somatic Ca2+ channel current was inhibited by omega-agatoxin IVA at c

267 function, and the reduction in the potassium-channel current was remedied by the application of the p

268 ximal activation rate of ensembled single BK channel currents was in the submillisecond range at > or

269 Using kinetic modeling of one-channel currents, we attributed the observed current dec

270 In detached, inside out patches single-channel currents were abolished by [Au(CN)2]- and activi

271 and alpha4beta2delta(R220H) receptor single-channel currents were both significantly decreased compa

272 tivation and the deactivation rate of mutant channel currents were characterized using the two-microe

273 KcsA channel currents were effectively blocked by potassium s

274 Channel currents were identified as K(ATP) channels thro

275 cGMP- and Ca(2+)-dependent channel currents were inhibited by 10 microM ZnCl(2) but

276 DiC8-PIP(2)-evoked channel currents were inhibited by anti-TRPC1 antibodies

277 RyR2 channel currents were measured in planar lipid bilayers.

278 BK channel currents were measured using whole-cell patch cl

279 Single channel currents were recorded in cell-attached patches

280 The channel currents were recorded under steady-state condit

281 ious work has suggested that subthreshold K+ channel currents were responsible for this dichotomous b

282 l potential and mean open time of the single-channel currents were similar in the absence or presence

283 alpha9alpha10 nAChRs but not N-type calcium channel currents, whereas [2,8]-dicarba analogues displa

284 operty of HCN channels is their small single-channel current, which is below the thermal noise thresh

285 ions in this gene produced reduced potassium-channel current, which was successfully remedied by phar

286 ',N'-tetraacetic acid (BAPTA-AM) both evoked channel currents, which had unitary conductances of appr

287 onstrated that D1R stimulation increased HCN channel current, while local HCN channel blockade in pri

288 tro recordings tested for D1R actions on HCN channel current, while recordings in monkeys performing

289 d high calcium permeability and large single-channel currents, while cells with mutant Tmc1 had reduc

290 d a concentration-dependent inhibition of BK channel currents with 3 mM producing approximately 50% i

291 o quiescent inside-out patches evoked single channel currents with a unitary conductance of 1.9 pS.

292 ET-1 evoked non-selective cation channel currents with a unitary conductance of 2.6 pS wh

293 e extracellular solution also reduced single-channel currents with an affinity that increased as [K(+

294 8810 (FP receptor antagonist)-insensitive BK channel currents with EC(50)s of 0.51 +/- 0.03 nM (n = 5

295 We examined GluN1/GluN2A single-channel currents with kinetic analyses and modeling to p

296 AMPAR/TARP combinations gave rise to single-channel currents with multiple conductance levels that a

297 racellular Ca2+ stores also activated cation channel currents with similar properties to Icat2.

298 out patches, bath application of PDBu evoked channel currents with similar properties to SOCs which w

299 ll-attached or inside-out patches, activated channel currents with similar properties to SOCs.

300 the microsomes harboring KcsA showing single-channel currents with the typical biophysical characteri