ライフサイエンスコーパス: channeling

1 es) and particle interactions (which reduces channeling).

2 metabolic enzymes communicate via substrate channeling.

3 eriments, additional evidences for substrate channeling.

4 This stepwise process is termed substrate channeling.

5 eviated by co-incubation with oleate via TAG channeling.

6 ferase (GatDE) function sequentially without channeling.

7 OXPHOS supercomplexes and promotes metabolic channeling.

8 lusive form of PutA that exhibits suboptimal channeling.

9 he role of the supercomplexes extends beyond channeling.

10 catalysis and in the regulation of substrate channeling.

11 atics plays an essential role in the APS(2-) channeling.

12 g suggestions of steric shielding and oxygen channeling.

13 interdomain tunnel associated with substrate channeling.

14 ations are discussed with respect to ammonia channeling.

15 ear evidence against dihydrofolate substrate channeling.

16 sites to exogenous APS all argue against APS channeling.

17 d the controversial phenomena of direct NADH channeling.

18 ing a role in adenosine phosphosulfate (APS) channeling.

19 nes and factor V(a) have been shown to cause channeling.

20 beta305 or Asp alpha56 to the regulation of channeling.

21 nce of the glucosyltransferase for metabolic channeling.

22 lies a selection pressure favoring substrate channeling.

23 mental confirmation of this structure or the channeling.

24 tatic-mediated and proximity-based substrate channeling.

25 leased into the bulk medium, i.e., substrate channeling.

26 ux control analysis provides no evidence for channeling.

27 Channeling a good catalyst: Highly porous palladium nano

28 This strategy entails a selective condition "channeling" a karyotypically divergent population into o

29 lding the same efficiency benefits as direct channeling, a well-known mechanism in which enzymes are

30 ne of two released intermediates or through "channeling" (activation without the release of an interm

31 ertheless, CfPutA exhibited normal substrate-channeling activity, indicating that it isomerizes into

32 the leucine substitution directing substrate channeling along different reaction channels in a simila

33 s from the membrane-V(a)-X(a) complex (i.e. "channeling") also was adjusted because both the membrane

34 dynamics simulations in studies of substrate channeling, also provides new insights into the molecula

35 tion of ammonia and ring formation in IGP by channeling ammonia from one remote active site to the ot

36 lution, raising the possibility of substrate channeling among the active sites in these two proteins.

37 UmuD was also shown to be capable of channeling an excess of UmuD' to ClpXP for degradation,

38 in the DmpF protomer may be correlated with channeling and activity at the DmpG protomer.

39 ticated catalytic concepts such as substrate channeling and allosteric communication existed already

40 osteric interactions that regulate substrate channeling and catalysis in the tryptophan synthase bien

41 of reactions involving amino acid substrate channeling and enzymatic multimodule skipping and iterat

42 cations of these findings both for substrate channeling and for catalysis are discussed.

43 thout a lag phase, consistent with substrate channeling and implying that the observed tunnel is func

44 o understand coclustering-mediated metabolic channeling and its application to both efficiency improv

45 hysically associated to facilitate substrate channeling and overcome diffusive barriers.

46 ng and kinetic characterization of substrate channeling and quinone reduction.

47 ein interactions that would enable substrate channeling and regulation of the metabolic flux.

48 ther approaches provided strong evidence for channeling and transient complex formation between A. ae

49 te brines might be the cause of the observed channeling and weathering in the surface.

50 BchJ may play an important role in substrate channeling and/or regulation of Chl biosynthesis but sho

51 basis for exploring mechanisms of catalysis, channeling, and allosteric communication in the tryptoph

52 ortant information regarding the processing, channeling, and functions of plant siRNAs.

53 development of a porous altered layer, flow channeling, and increasingly limited calcite dissolution

54 9 in the context of light harvesting, energy channeling, and photoprotection within photosystem II.

55 nal SK in catalyzing the kinase reaction and channeling APS is illustrated by the strict requirements

56 Potential underlying causes of activation of channeling are discussed.

57 is and find that the conclusion of substrate channeling arises from the particular behavior of a sing

58 TS calls into question surface electrostatic channeling as the universal means of dihydrofolate trans

59 A channeling assay that optically detects solution-phase A

60 ely 50% of the substrate to be activated via channeling at 50 microm membrane concentration, but fact

61 In this way, metabolic channeling at the entry point into isoflavonoid phytoale

62 discussed in relation to potential metabolic channeling at the entry point into the isoflavonoid path

63 iChS by chemical modifications favors anion channeling at the expense of substrate transport, sugges

64 hominis TS-DHFR kinetic mechanism, substrate channeling behavior, and domain-domain communication.

65 There is a preference for GAP channeling between aldolase and GAPDH when compared to b

66 spiratory chain function by allowing quinone channeling between complexes I and III.

67 Our results provide evidence for substrate channeling between hBCATm and BCKD complex and formation

68 Channeling between PAL and C4H was confirmed in vitro in

69 effort to probe the possibility of substrate channeling between the TS and DHFR active sites and to i

70 te of reduction (19 min (-1)), suggesting no channeling between the two active sites.

71 h to be considered as a theoretical proof of channeling between these proteins.

72 Notably, substrate "channeling" between the enzymes in supercomplexes has be

73 s observed with anti-TNF therapy may reflect channeling bias, whereby patients with the highest risk

74 To decrease potential "channeling" bias, the study included only new episodes o

75 netics of the bisynthase, not only by steric channeling but also by preferential escape of the produc

76 One solution is metabolic channeling by forming a transitory HpaB-HpaC complex.

77 LDH) through the control of NAD(+) substrate channeling by specifically shifting NAD(+) between the t

78 By highlighting how substrate channeling can allow PKS modules to effectively accept a

79 as a lid that covers the internal substrate channeling cavity, thus preventing escape of the catalyt

80 nteraction between CypD and the IP3R1 Ca(2+) channeling complex increased concomitantly with mitochon

81 protein-protein interaction of the substrate channeling complex with the pleiotropic protein kinase,

82 was built for a proposed PRODH-P5CDH enzyme channeling complex.

83 mate uptake assays, we identified a chloride-channeling conformer, iChS, transiently accessible as EA

84 Shortcomings in study design, including channeling, confounding bias, and study heterogeneity, m

85 h resolution X-ray diffraction, and electron channeling contrast imaging.

86 This channeling determines the nature of the subsequent repai

87 AH1-encoded phosphatidate phosphatase and by channeling diacylglycerol to phosphatidylcholine via the

88 Moreover, RMH-1 also has a major role in channeling DSBs into an NCO HR outcome near the centers

89 substrate-level phosphorylation), substrate channeling (e.g., of oxaloacetate from malate dehydrogen

90 The statoliths accelerated sooner, and the channeling effect was less pronounced in roots treated w

91 lower but significant electrostatic-mediated channeling efficiency (?15-25%) at physiological pH (7.0

92 ing of the intermediate at 37 degrees C, but channeling efficiency increases dramatically at elevated

93 Channeling efficiency is dependent upon a conformational

94 nd TS active sites significantly reduces the channeling efficiency of P. falciparum DHFR-TS.

95 t organisms capable of forming a biofilm and channeling electrons from substrates to the anode at hig

96 intermediates for biosynthetic pathways and channeling electrons to the respiratory chain complexes.

97 e high affinity for substrates combined with channeling ensures the efficient transfer of carbamoyl p

98 c tissues as well as cellular mechanisms for channeling excess fatty acid to particular metabolic fat

99 Channeling fatty acid substrates into TG resulted in dec

100 that each isoform has distinct functions in channeling fatty acids into different metabolic pathways

101 f THF-activated one-carbon unit synthesis by channeling folate cofactors to other folate-dependent en

102 The pol beta ACF was capable of substrate channeling for steps in vitro BER and was proficient in

103 tures and cell spreading and reveal a signal-channeling function for a MEK1/ERK scaffold in orchestra

104 NPP1 (Enpp1(-/-)), or defective in the PP(i) channeling function of ANK (ank/ank), have decreased lev

105 lar dynamic simulations that investigate the channeling function of hisF.

106 or glucokinase activator, which resulted in channeling glucose/G6P toward glycolysis and lipid synth

107 n suggest two potential physiological roles: channeling GS-hydroquinones back to hydroquinones and re

108 -type and mutant GSAT for binding to GTR and channeling GSA from GTR to GSAT.

109 Metabolic channeling has been proposed to occur at the entry point

110 Substrate channeling has previously been shown for bifunctional Pu

111 , discovering a process we termed "substrate channeling." Here, we investigate the protein-protein in

112 ur findings do not support the electrostatic channeling hypothesis.

113 ing BMI coupled with the luminescence oxygen channeling immunoassay (LOCI).

114 kinetic benefit of linker-mediated substrate channeling in a modular PKS.

115 e, we report the first evidence of substrate channeling in a PRODH-P5CDH two-enzyme pair.

116 sient kinetics approaches developed to study channeling in bienzyme complexes to an archaeal protein

117 sed on the well-known mechanism of substrate channeling in enzyme complexes, SA-induced H2O2 accumula

118 Allosteric interactions regulate substrate channeling in Salmonella typhimurium tryptophan synthase

119 ian respiratory chain that depend on whether channeling in supercomplexes is kinetically important, a

120 enesis to test the hypothesis that substrate channeling in the bifunctional thymidylate synthase-dihy

121 s the first structural evidence of substrate channeling in the Krebs cycle metabolon.

122 site coupling suggests a role for substrate channeling in the PDE-dependent dissociation and hydroly

123 direct "channeling." Our results reveal cAMP channeling in the PDE8-RIalpha complex and a molecular d

124 Disrupting gas channeling in the soil improved mass transport of O3 to

125 des structural evidence for direct substrate channeling in the supercomplex assembly with short diffu

126 Substrate channeling in the tryptophan synthase bienzyme complex f

127 Substrate channeling in the tryptophan synthase bienzyme complex i

128 Substrate channeling in the tryptophan synthase bienzyme is regula

129 There was no channeling in the usage of selective COX-2 inhibitors to

130 lity of electrostatically mediated substrate channeling in vitro and in vivo.

131 to the electrostatically mediated substrate channeling in vitro, in situ, and in vivo.

132 extensively documented example of substrate channeling in which the oligomeric unit has been describ

133 work also highlighted the need for substrate channeling (in human) and ATP hydrolysis (in yeast) for

134 RBS ion-channeling including angular scans confirm that Sn occup

135 icating that the mutants disrupt PRODH-P5CDH channeling interactions.

136 ing this process is essential to prevent its channeling into toxic structures threatening cell viabil

137 Metabolite channeling is a common feature of multifunctional enzyme

138 e ferritin core, we propose that ferric iron channeling is a conserved feature of iron homeostasis in

139 e conditions, in the bifunctional enzyme and channeling is not observed between AICAR Tfase and IMP C

140 riodic feedwater switching, and preferential channeling is observed in the latter.

141 ulations demonstrate that the probability of channeling is strongly dependent on the concentration of

142 ging strategy to promote efficient substrate channeling is to spatially organize pathway enzymes in a

143 of Sgs1 in meiotic recombination, including channeling JMs into physiological crossover and noncross

144 -enzyme interactions and direct intermediate channeling, leading to a decrease in the overall reactio

145 luded involvement of the covalent, substrate-channeling mechanism by which enzyme-bound intermediates

146 This channeling mechanism could facilitate the consecutive re

147 monofunctional enzymes utilize an analogous channeling mechanism has not been examined.

148 rotein-protein interactions in the substrate-channeling mechanism has prompted us to develop novel as

149 membranous forms of DbetaM, if an efficient channeling mechanism is operative in vivo, soluble Dbeta

150 thermophilus are consistent with a substrate channeling mechanism, as the approach to steady-state fo

151 Kinetic data for SmPutA indicate a substrate-channeling mechanism, in agreement with previous studies

152 rmin or phenformin) initiating the substrate channeling mechanism, the catalytic subunit of CK2 (CK2a

153 Ftr1p for permeation by a classic metabolite channeling mechanism.

154 ake with a kinetic pattern consistent with a channeling mechanism.

155 Therefore, channeling mechanisms enabling specific protein-protein

156 portant for the accumulation of ChlH and for channeling metabolites into the chlorophyll biosynthetic

157 he substrate is presented to the module in a channeling mode as opposed to a diffusive mode.

158 DEBS modules when primed in a more "natural" channeling mode by the same panel of diketide substrates

159 ArgRS, exactly as would be predicted by the channeling model for mammalian translation.

160 Given this evidence, we propose a substrate channeling model for the efficient conversion of dimethy

161 ficantly to global biogeochemical budgets by channeling nutrients through alternate trophic pathways.

162 These findings are consistent with channeling of 10:0 into TAG through the combined activit

163 f enzymatic reactions may enhance the direct channeling of a variety of substrates.

164 ddition of l-carnitine enabled the metabolic channeling of acyl-CoA through carnitine palmitoyltransf

165 alpha)(8) in the closed state may assist the channeling of ammonia at the interface of the two functi

166 ant were consistent with the model where the channeling of ammonia has been disrupted due to the leak

167 The direct channeling of an intermediate between enzymes that catal

168 n toward and across the midline, and (3) the channeling of axons toward particular regions.

169 results provide definitive evidence for the channeling of beta-aspartyl phosphate between aspartokin

170 intermodular "linker pairs" in the selective channeling of biosynthetic intermediates between adjacen

171 se of BCKDC, forming a metabolon that allows channeling of branched-chain alpha-keto acids from BCATm

172 ole for LCB Delta8 unsaturation in selective channeling of ceramides for the synthesis of complex sph

173 nd IV is about 6 nm, which supports proposed channeling of cytochrome c between the individual comple

174 objective of understanding the electrostatic channeling of dihydrofolate generated at the TS active s

175 oil of the PDAT overexpressors is due to the channeling of fatty acids from phosphatidylcholine into

176 Such cellular events include excessive channeling of glucose intermediaries into various metabo

177 sm through stimulation of glucose uptake and channeling of glucose intermediates into the hexosamine

178 al benefit of this form of metabolism is the channeling of glucose into biosynthetic pathways.

179 onstitutive synthesis of GlpD and subsequent channeling of glycerol-3-phosphate into metabolic pathwa

180 3 plant, low temperature also influences the channeling of glycerolipids from the ER to chloroplast.

181 physical and functional complex that allows channeling of GSA between the enzymes.

182 on is thought to represent reduced metabolic channeling of HCO3- to the intracellular HCO3- binding s

183 riments reveal an entropic trap that ensures channeling of highly mobile protons along the membrane i

184 abolic network with multiple IAN sources and channeling of IAOx-derived IAN into camalexin biosynthes

185 ar concentration of active Ste5 enhanced the channeling of inappropriate stimuli to Fus3.

186 The channeling of indole between the alpha- and beta-sites v

187 r mechanisms of attention and how it affects channeling of information in different neuronal circuits

188 dent sequential enzyme activities, including channeling of intermediate and conformational change.

189 exes, or metabolons, to facilitate efficient channeling of intermediates and to prevent undesired met

190 ompelling evidence in favor of the so-called channeling of intermediates between enzyme active sites

191 a supramolecular complex, in which there is channeling of intermediates between enzyme active sites.

192 whereas matched sequences can facilitate the channeling of intermediates between ordinarily nonconsec

193 extensively explored, their relevance to the channeling of intermediates in metabolism is not widely

194 loroplast stroma to facilitate the efficient channeling of intermediates through the pathway.

195 be constructed, providing for the efficient channeling of intermediates to final products, and if th

196 c models, most interestingly, voltage-driven channeling of ions through a pair of activation-energy b

197 yase (ASL) is required for the synthesis and channeling of L-arginine to nitric oxide synthase (NOS)

198 s vacuolation and clustering, leading to the channeling of large PI(3)P-positive membranes to CCVs fo

199 de a mechanism for the sorting and selective channeling of lipid precursors between the galactolipid

200 creased its specific activity and stimulated channeling of long-chain base precursors into sphingolip

201 sulates riboflavin synthase (BsRS), enabling channeling of lumazine from the site of its generation t

202 A selective channeling of LVST inputs through segmentally and dorsov

203 mbering (sn)-1 position from PC and likely a channeling of lysophosphatidic acid, derived from PC, in

204 titive, and non-competitive) mechanisms, the channeling of metabolic flow through isozymes, the chann

205 ling of metabolic flow through isozymes, the channeling of metabolic flow via transamination reaction

206 Channeling of metabolic intermediates via enzyme complex

207 ylcarnitine and ketone bodies, and (iii) the channeling of mitochondrial acetyl-CoA from pyruvate deh

208 The efficiency of channeling of NH(3) for synthesis of PRA was found to in

209 GPAT activity is known to depend upon channeling of NH(3) from a site in an N-terminal glutami

210 This finding has led to the proposition that channeling of NO into SNOs may provide a natural defense

211 xperiments appear high enough to abolish any channeling of oxaloacetate.

212 lakoid membrane, possibly to allow metabolic channeling of potentially phototoxic Chl breakdown inter

213 The channeling of recombination from reciprocal exchange to

214 e findings identify a unique pathway for the channeling of small lymph-borne antigens and chemoattrac

215 different molecular mediators and facilitate channeling of specific signaling pathways to the cell cy

216 ial reactions is often envisaged to occur by channeling of substrate between enzyme active sites with

217 organization, called a metabolon, results in channeling of substrates between enzymes.

218 nor to these key enzymes, therefore enabling channeling of sulfur substrate in the cell as well as gr

219 y Ovadi et al. are consistent with a partial channeling of the intermediate at 37 degrees C, but chan

220 The potential channeling of the intermediate beta-aspartyl phosphate b

221 ) There was no kinetic evidence of substrate channeling of the intermediate, the formylaminoimidazole

222 this macromolecular machine by promoting the channeling of the intermediates of catalysis between the

223 transfer from ArsD to ArsA, consistent with channeling of the metalloid from one protein to the othe

224 This results from the channeling of the propionyl moiety of heptanoate into an

225 Brownian dynamics (BD) simulations test for channeling of the substrate, glyceraldehyde 3-phosphate

226 dietary long chain fatty acids is related to channeling of these lipids to distinct cellular metaboli

227 fursome and thus enables a global long-range channeling of tRNA(Cys) between SepRS and SepCysS distan

228 nd facilitate activation, which involves the channeling of two nickel ions into the active site.

229 ecognition, deubiquitylation, unfolding, and channeling of ubiquitylated substrates to the 20S protea

230 "Channeling" of RA between CRABP-II and RAR markedly faci

231 in efficiency by restricted diffusion (i.e., channeling) of an intermediate APS(2-) between active si

232 o explore the question of whether there is a channeling or "hand-off" of the repair intermediates dur

233 the hydrolysis site on PDE8 through direct "channeling." Our results reveal cAMP channeling in the P

234 ed in processes associated with the sorting, channeling, packaging, and transport of synaptic materia

235 acids rescue palmitate-induced apoptosis by channeling palmitate into triglyceride pools and away fr

236 The structural model predicts that the core channeling pathway of bifunctional PutA enzymes is conse

237 be caused by the slow leakage of DA from the channeling pathway to the ghost interior.

238 Nevertheless, the functional consequences of channeling peripheral taste signals through inappropriat

239 ted protein kinases (MAPKs), responsible for channeling physiological and environmental signals to th

240 These data suggest that interstitial fluid channeling precedes and may even direct lymphangiogenesi

241 This article describes modeling of the whole channeling process by numerical solution of the Smolucho

242 rse-grained Brownian dynamics, the substrate channeling process has been studied with reactions in mu

243 rafficking pathway as would be expected in a channeling process.

244 (Dlg1) specifies TCR-dependent functions by channeling proximal signals toward the activation of p38

245 studies revealed that CRABP-II functions by "channeling" RA to RAR, thereby enhancing the transcripti

246 These results demonstrate that channeling Ras-dependent signals to PI3K confers TSH wit

247 ) and Rutherford backscattering spectrometry channeling (RBS-C) spectra show that the range of radiat

248 of the readily releasable pool of SVs and in channeling retrieved SVs toward direct recycling rather

249 fasciculation of the POC, consistent with a ;channeling' role for these repellent molecules.

250 nization of HIV-1 particles and suggest that channeling serine into lipid biosynthesis may not be a c

251 ceptors, precluding G protein activation and channeling signaling to alternative pathways.

252 aldolase/GAPDH complex were compared to the channeling simulations.

253 ns produced changes consistent with impaired channeling, so our findings do not support the electrost

254 plasma membrane may provide a mechanism for channeling specific stimuli into distinct signaling path

255 increased significantly in K562/IMA-1 cells, channeling sphingoid bases to the sphingosine kinase pat

256 ts suggest that EcPutA achieves an activated channeling state during the approach to steady state and

257 Instead, a combination of oxygen channeling, steric shielding, and selective radical trap

258 ngs are consistent with a role for Sema6A in channeling sympathetic axons into the sympathetic chains

259 ied cell model with a creatine kinase energy channeling system.

260 nificant mid-gap states and different charge-channeling tendencies.

261 ues important for ferritin substrate-product channeling that, when identified, will outline the entir

262 rotects alginate from degradation by AlgL by channeling the alginate polymer through the proposed alg

263 ation products derived from omega-6 PUFAs by channeling the free radical pathway away from the F2-Iso

264 arget protein by SPR for confirmation before channeling the hits into Nuclear Magnetic Resonance (NMR

265 n alternative mechanism for potentiating and channeling the immune response.

266 Therefore, elucidating the mechanism of channeling the intracellular effect of RTK stimulation t

267 omplex chemical synthesis can be achieved by channeling the reactivity of highly unsaturated molecule

268 se that sFRPs can act as a molecular switch, channeling the signal input for different non-canonical

269 receptors, blocking G protein activation and channeling the signaling to G protein-independent pathwa

270 Because of this channeling, the labeling of acetylcarnitine and ketone b

271 that are believed to contribute to acyl-CoA channeling, the metabolic consequences of loss of these

272 part by regulating intracellular fatty acid channeling, Them2 functions in brown adipose tissue to s

273 he relationship between our findings and the channeling theory of translational efficiency is discuss

274 presence of (*)OH and Mn(IV)-oxo species by channeling these oxidants toward the synthesis of a func

275 been achieved due to the prominent electron channeling through the pores of polymeric nano-crystalli

276 ulses are applied only when microbubbles are channeling through the thrombus.

277 t factor V(a) extends the range of efficient channeling to much lower or higher membrane concentratio

278 nd, thus, the potential for selective signal channeling to segments of the transcriptional network.

279 lation of cognate tRNAs and their subsequent channeling to the ribosome.

280 Our results indicated that dihydroflavonol channeling toward anthocyanin production was boosted dur

281 bstrate binding loop and seals the substrate-channeling tunnel via tertiary structural interactions t

282 y-state formation of NADH does not fit a non-channeling two-enzyme model.

283 hich helps to control the damage response by channeling ubiquitin from the proteosomal degradation pa

284 4-epimerase gene, UGE4, which is involved in channeling UDP-D-galactose (UDP-D-Gal) into cell wall po

285 se synthase, we discuss its proposed role in channeling UDP-Glc to cellulose synthase during secondar

286 thus alleviates PSII excitation pressure by channeling up to 30% of PSII-originated electrons.

287 e analysis showed formation of electrostatic channeling upon protein-protein association, which is th

288 The mutants were assessed for impaired channeling using two criteria: a lag in product formatio

289 Channeling was not detected in two of the three types of

290 Kinetic evidence for substrate channeling was sought by measuring the time courses for

291 tas comprising a large interlinked waterway, channeling water and animals into and across the Sahara,

292 r SGLT1 in either pumping water or passively channeling water contrast with its reported water transp

293 cinnamate 4-hydroxylase (C4H), to facilitate channeling, we generated transgenic tobacco (Nicotiana t

294 uct suggests a remarkable form of metabolite channeling where the next enzyme in the biosynthetic pat

295 )) and via the same reaction pathway (50-60% channeling, with the rest via the meizothrombin intermed

296 lar dynamics and metadynamics to investigate channeling within DmpFG in both the presence and absence

297 o membrane, our findings support hydrophobic channeling within membranes as a means of cellular and t

298 model of allosteric regulation and substrate channeling within protein complexes.

299 scuss these data in the context of substrate channeling within the Golgi.

300 We found that substrate channeling within this enzyme is energetically feasible