ライフサイエンスコーパス: chaotrope

1 to bind Fg when they were destabilized by a chaotrope.

2 arent weakening of the hydrophobic effect by chaotropes.

3 mental changes such as low concentrations of chaotropes.

4 LC, gel electrophoresis, and dissociation by chaotropes.

5 in vivo as compared with amyloids formed in chaotropes.

6 eractions driven either by the presence of a chaotrope (a nonspecific trigger for aggregation) or by

7 1, and SOSIP.664 trimers) to explore how the chaotrope acts.

8 are compatible with solubility agents (e.g., chaotropes and detergents) known to improve enzymatic di

9 Protein conformational stabilities against chaotropes and temperature were measured.

10 sion of kosmotropes from (or accumulation of chaotropes at) the surface, the kinetics proved to be mo

11 osmotropic anions "make" water structure and chaotropes "break" it being countered by recent studies

12 Arginine is a well-known chaotrope but increases surface tension of water.

13 cts of a range of temperature, pressure, and chaotrope concentrations were tested and led to optimal

14 elongation, whereas poorly hydrated anions (chaotropes) delay nucleation and mildly affect the elong

15 Chaotrope denaturation of TTR exhibits unusual behavior

16 Chaotrope-denatured amylin and amylin processing interme

17 asurements of the SCN(-) ion, a prototypical chaotrope, determined a free energy of adsorption within

18 (SPE) processes, a silica solid phase using chaotrope-driven binding and an ion exchange phase using

19 ls expressing CB(1) and CB(2) receptors were chaotrope extracted to denature endogenous GTP-binding p

20 developed an in situ reconstitution assay in chaotrope-extracted membranes from mouse fibroblasts exp

21 chaotropic from kosmotropic substances (i.e. chaotropes >/= +4; kosmotropes </= -4 kJ kg(-1) mol(-1)

22 oxide dismutase (SOD1) dimers induced by the chaotrope guanidine hydrochloride (GdnHCl) or the reduct

23 Structural stability studies with the chaotrope guanidinium chloride revealed that there is mo

24 BioI secondary and tertiary structure by the chaotrope guanidinium chloride.

25 the precipitation of proteins, while others (chaotropes) increase their solubility.

26 ons the net effect of the linear, presumably chaotrope-induced deceleration and the presumed, square

27 Each chaotrope induces dissociation of five subunits in a con

28 onization of the peptides (e.g., detergents, chaotropes, inorganic salts).

29 with respect to denaturation by heat and by chaotropes is presented.

30 indirect mechanism, in which urea acts as a chaotrope, is not a likely cause of urea's action as a d

31 LAP was refolded to its dimeric form using a chaotrope-mediated folding procedure.

32 reatment at alkaline pH ( > or = 10) or with chaotropes (NaBr, Nal, and urea), suggesting that these

33 of the effects of pressure, temperature, and chaotrope on unfolding of rhGH documented that under con

34 e surfactants, two organic solvents, and two chaotropes on the enzymatic digestion efficiency of memb

35 ples of chao-/kosmotropicity values are, for chaotropes: phenol +143, CaCl(2) +92.2, MgCl(2) +54.0, b

36 In combination, these results suggest that chaotropes primarily induce protein solubilization by di

37 f-denaturation and upon the concentration of chaotrope required to half-denature the tracer at consta

38 The addition of the charged chaotrope SCN(-) in 150 mM concentration improves the re

39 We conclude that the chaotrope sensitivity of antibody binding to Env depends

40 ants, either in buffer or in the presence of chaotropes such as thiocyanate, establish the presence o

41 of cell lysates with high concentrations of chaotropes such as urea.

42 antibody binding to Env antigens by an added chaotrope (such as thiocyanate).

43 ssment was possible without the use of added chaotropes, such as urea, to dissociate GroEL.

44 these data, we suggest that dilution of the chaotrope to initiate refolding will result in collapse

45 uanidine HCl and refolded by dilution of the chaotrope to minimally denaturing conditions and disulfi

46 tes assayed (from +361 kJ kg(-1) mol(-1) for chaotropes to -659 kJ kg(-1) mol(-1) for kosmotropes) bu

47 Chaotrope treatment of the paired grana membranes has al

48 ctural epitopes is particularly sensitive to chaotrope treatment, whereas antibody binding to epitope

49 lpy and entropy changes of this prototypical chaotrope were both determined to be negative.