ライフサイエンスコーパス: chaotropic

1 from hydrophobic substances that exert their chaotropic activity, by proxy, from within the hydrophob

2 buffer with a fixed optimal concentration of chaotropic agent (e.g., 1.2 M urea) to differentially is

3 The chaotropic agent Gdn lowered the affinity of interaction

4 n vitro, such as elevated temperatures and a chaotropic agent guanidine hydrochloride, were studied.

5 The chaotropic agent urea (at low concentrations) does not a

6 Low concentrations of the chaotropic agent urea do not affect native Env but desta

7 ions, including GuH(+), which also acts as a chaotropic agent, affect the equilibrium between the ope

8 as a cation, stimulating activity, and as a chaotropic agent, altering the distribution of conformat

9 in-lacking mitochondria in the presence of a chaotropic agent, which disrupts the interaction between

10 w temperature, Env-activating ligands, and a chaotropic agent.

11 and 31 nM in the absence or presence of anti-chaotropic agents (10% glycerol and 0.5 M Na2SO4), respe

12 lvents (acetonitrile, methanol, 2-propanol), chaotropic agents (6 M urea, 6 M guanidine-HCl), a prote

13 Chaotropic agents (e.g., salts, trehalose, urea, tempera

14 from isolated tonoplast preparations by mild chaotropic agents and thus appeared to be nonintegrally

15 -resistant oligomers and filaments stable to chaotropic agents are detected using all Tyr --> Phe alp

16 The binding is irreversible in vivo but chaotropic agents disrupt the association between the no

17 When a sample mixed with chaotropic agents is filtered through the nucleic acid i

18 60-fold stabilization of the dimer, the anti-chaotropic agents produced a 7-fold enhancement in the c

19 itro method to desaturate LH-RH receptors by chaotropic agents such as manganous chloride (MnCl2) and

20 In the presence of the chaotropic agents urea and guanidine HCl, the movement o

21 Improved stability toward chaotropic agents was observed for both mutants, but no

22 ronment (changes in temperature, addition of chaotropic agents), no isolated subunits in native condi

23 els of inorganic salts, buffers, detergents, chaotropic agents, and other solubilizing agents.

24 els of inorganic salts, buffers, detergents, chaotropic agents, and other solubilizing agents.

25 nanodiamonds and separating out detergents, chaotropic agents, and salts, and other impurities that

26 mbranes after treatment with Triton X-100 or chaotropic agents, implying a stabilizing role for PsaD.

27 ed pH range, fail to work in the presence of chaotropic agents, or are difficult to use.

28 complex mixture of inorganic salts, buffers, chaotropic agents, surfactants/detergents, preservatives

29 In the presence of chaotropic agents, the wild type released a large (>150-

30 is dimer is affected by the presence of anti-chaotropic agents.

31 etergent with high salt concentration and in chaotropic agents.

32 in the Y42F mutant can be interconverted by chaotropic and kosmotropic agents, respectively, accordi

33 glutamate aminopeptidase requires the use of chaotropic and reducing agents, quite often with limited

34 temperatures and polysorbate 20, rather than chaotropic and reducing agents, with sample cleanup and

35 rV lambda 6Wil and -Jto were determined from chaotropic and thermal denaturation studies.

36 iated less than were glial currents when the chaotropic anion thiocyanate was substituted for glucona

37 Here we report a surprising chaotropic-anion-induced micellization of cationic ammon

38 Unlike chaotropic anions (e.g. ClO4(-)), kosmotropic anions (e.

39 e, we present the first direct evidence that chaotropic anions have an affinity for hydrophobic conca

40 tion can be controlled by the application of chaotropic anions in the mixture, allowing a second leve

41 Chaotropic anions provided the lowest detection limits,

42 d for recycling of Em is activated by Pi and chaotropic anions such as azide and thiocyanate, giving

43 These transporters exhibit a permeability to chaotropic anions that is associated with transport, all

44 Analyses of cells treated with the highly chaotropic bile salt detergent deoxycholic acid demonstr

45 ) compared to that with a silica phase under chaotropic conditions (53%).

46 Under chaotropic conditions, DNA released from lysed cells cau

47 solubilized by 0.6 M NaCl and requires more chaotropic conditions, such as 0.5 M KI.

48 ermore, treatment of pure protein with acid, chaotropic denaturants, alkylators, and detergents faile

49 The modified peptide is more resistant to chaotropic denaturants, and the free energy of folding o

50 However, the "end-states" of temperature and chaotropic denaturation are spectroscopically differenti

51 A chaotropic detergent, 0.1% Nonidet P-40, also abolished

52 a kinetic trap from which it can escape via chaotropic, detergent, or thermal perturbation.

53 well fitted as a sum of a linear, presumably chaotropic effect and a square root-dependent, presumabl

54 disorder the site of hairpin interaction by chaotropic effects.

55 xigenic Escherichia coli (ETEC) toxins after chaotropic extraction of DNA from stool would increase t

56 but there are key points that delineate (i) chaotropic from kosmotropic substances (i.e. chaotropes

57 A chaotropic guanidine-based method for histone isolation

58 ent denaturation behaviors under thermal and chaotropic (guanidinium chloride) stress.

59 arried out in the presence of thiocyanate, a chaotropic ion that markedly increases the affinity of A

60 enhance cation exclusion effects and reduce chaotropic/ion pairing effects by using (1) low ionic st

61 (dihydrogen phosphate) that exhibits minimal chaotropic/ion pairing effects, and (3) columns that sho

62 simulations and experiments have shown that chaotropic ions actually exhibit enhanced concentrations

63 indicated strong binding of weakly hydrated chaotropic ions.

64 Chaotropic lysis and isopropanol precipitation were used

65 th a modification of the recently introduced chaotropic NaI method, reducing our estimate of the leve

66 ined, giving preliminary arguments about the chaotropic nature of the inorganic ions.

67 investigated the effects of either strongly chaotropic, nearly neutral or strongly kosmotropic salt

68 ly describe the chemical nature (kosmotropic/chaotropic or hard/soft) of the interacting species.

69 This effect is generally attributed to chaotropic or ion pairing effects.

70 to changes in its local structure by either chaotropic or kosmotropic ions.

71 Chaotropic perturbation (CP) with guanidinium chloride (

72 MCF-7 cells also showed the requirement of a chaotropic protein denaturant in the assays and selectiv

73 poisoning by ICRF-193 required the use of a chaotropic protein denaturant in the topoisomerase poiso

74 urant SDS, but easily detectable with strong chaotropic protein denaturants.

75 alovirus (CMV) antigen preparations with the chaotropic reagent Urea and probing with immune sera fro

76 Treatments with chaotropic reagents (urea, NaI, or NaBr) or with alkalin

77 h-speed cell disruption (HSCD) incorporating chaotropic reagents and lysing matrices in comparison to

78 ding or by denaturation of the bound Ig with chaotropic reagents, but could be dissociated by reducti

79 This method replaces chaotropic reagents, such as urea and guanidine hydrochl

80 ing kinetics is more complex when the highly chaotropic salt guanidine hydrochloride (GuHCl) is emplo

81 e from the endoplasmic reticulum membrane by chaotropic salt wash, consistent with lack of a putative

82 Treatment of dynactin with the chaotropic salt, potassium iodide, completely depolymeri

83 i) the use of PCR-inhibiting reagents (e.g., chaotropic salts and alcohol) and (ii) the washing and e

84 lly used in DNA extraction procedures (e.g., chaotropic salts, 2-propanol) can be avoided, making the

85 es in the presence of high concentrations of chaotropic salts.

86 is quite similar to that in the presence of chaotropic sodium bromide (and other halides), which rai

87 otropes </= -4 kJ kg(-1) mol(-1) ); and (ii) chaotropic solutes that are readily water-soluble (log P

88 CFU/ml, respectively, for a glass matrix and chaotropic solution protocol; 8.0 x 10(2) and 7.0 x 10(3

89 d the effect of acid and other denaturing or chaotropic solvents on the conformation of monoclonal an

90 e sequential use of these three increasingly chaotropic solvents to elute antibodies results in both

91 otein splicing and unprecedented thermal and chaotropic stability.

92 alternative methods tested, the use of this chaotropic technique of DNA isolation by using NaI produ

93 nic generation (SHG) studies of the strongly chaotropic thiocyanate ion adsorbed to the interface for

94 lacking all tyrosine residues, was stable to chaotropic treatment.

95 mbrane-bound VP14 was partially resistant to chaotropic washes such as 0.1 M Na2CO3 (pH 11.5) and 6 M

96 ions favor native protein structure, whereas chaotropic (weakly water binding) cations enhance native