ライフサイエンスコーパス: charged residue

1 t concentration, due to Coulomb repulsion by charged residues.

2 m) abolished CaM binding without introducing charged residues.

3 ast, the same region comprises 17 positively charged residues.

4 ternary epitope containing a high density of charged residues.

5 by a linear fit to the density of positively charged residues.

6 that include both positively and negatively charged residues.

7 groove that is lined with several positively charged residues.

8 inked to the fourth channel by a 'funnel' of charged residues.

9 linear sequence distributions of oppositely charged residues.

10 ne helix, in a region loaded with negatively charged residues.

11 ents contain a variable number of positively charged residues.

12 of TFPI encompassing all 12 surface-exposed charged residues.

13 y structure occur close to internal polar or charged residues.

14 and d that are mediated by highly conserved charged residues.

15 including a repulsive stretch of positively charged residues.

16 o a high number of positively and negatively charged residues.

17 enced by the linear patterning of oppositely charged residues.

18 ontent, intermediate hydrophobicity, and few charged residues.

19 duction pathway lined by numerous positively charged residues.

20 osphosites that are surrounded by negatively-charged residues.

21 otential PKA site at Ser-938 and surrounding charged residues.

22 ormational changes in a network of conserved charged residues.

23 s of linker mutants revealed that a group of charged residues, (200)EKR(202), is crucial for the swiv

24 To test whether another negatively charged residue (a glutamic acid residue, E) could fulfi

25 tic interactions with neighboring negatively charged residues, a result also confirmed by disulfide t

26 Thus, reversal of charged residues about the conserved tropomyosin-binding

27 + T rich genome may influence how positively-charged residues accumulate in SLiMs.

28 els and enzymes by transporting electrically charged residues across a hydrophobic VSD constriction c

29 ostatic interactions between complimentarily charged residues across the interface between the N- and

30 ostatic interactions between complementarily charged residues across the interface between the N- and

31 First, charged residues act as anchors to establish the initial

32 Results confirm that charged residues adjacent to phosphorylation sites and r

33 Positively charged residues adjacent to phosphorylation sites in th

34 esis to probe the functional significance of charged residues adjacent to the phosphorylation sites a

35 ociation rate constants, whereas mutation of charged residues affects dissociation rate constants.

36 in this C-terminal tail balances positively charged residues, allowing a more compact ensemble of st

37 h the N-terminal sensor of BovK, and several charged residues and a conserved hydrophobic region in t

38 two transmembrane helices contain conserved charged residues and are implicated in substrate binding

39 se sites are highly enriched in prolines and charged residues and are strikingly similar to other lig

40 ism that excludes substrates with positively-charged residues and favors LAT and SLP-76 phosphosites

41 a balance between the numbers of positively charged residues and hydrophobic anchoring residues.

42 unusual protein contains numerous polar and charged residues and lacks characteristic membrane-inter

43 As a whole, these studies implicate charged residues and potential electrostatic interaction

44 We identify five charged residues and six uncharged residues at the inter

45 mportance of the rarely occurring negatively charged residues and the N-terminal coil region in tropo

46 e site contains several conserved positively charged residues, and a portion of the active site shows

47 ng a mix of bulky hydrophobic and positively charged residues, and an adjacent amphipathic region tha

48 Almost all proteins contain charged residues, and their chain distribution is tailor

49 H-NS N-terminal domain are unusually rich in charged residues, and their interaction is mostly electr

50 Once the charged residues are capped with CEs, certain charge sta

51 d their receptor, even where the glycans and charged residues are distant from the interface.

52 e closed state, the two outermost negatively charged residues are exposed to extracellular fluid and

53 t in the SH3-like domain of NdhS, where nine charged residues are highly conserved among plants.

54 chanism becomes YidC-dependent if negatively charged residues are inserted into the translocated peri

55 quence of the linker reveals that positively charged residues are separated with a typical helical pe

56 estrict DNA interactions with the positively charged residues Arg-14 and Lys-17.

57 that substitution of a conserved positively charged residue (Arg-388, hEAAT1) in transmembrane domai

58 importance of this region, three positively charged residues (Arg 86, Arg 91, Lys 100) and the ancho

59 We have identified two charged residues (Arg(145) and Lys(153)) within this seg

60 Single alpha-helix (SAH) domains are rich in charged residues (Arg, Lys, and Glu) and stable in solut

61 how that the weaker densities for negatively charged residues arise from their greater sensitivity to

62 ne contains almost three times the number of charged residues as the internal side of the outer membr

63 le side chain of Trp-1126 and the negatively charged residues Asp-1129, Asp-1131, and Asp-1133.

64 Charged residues Asp-997, Glu-998, Arg-1000, and Lys-100

65 d that the activation process is hindered by charged residue associations as well as by local steric

66 However, a negatively charged residue at position 180 was necessary to convey

67 ical face four of HLA-DRbeta together with a charged residue at the boundary with the stalk region al

68 In contrast, substituting a negatively charged residue at the equivalent position in Kir7.1 enh

69 Mutation of a charged residue at the interface (Arg-103) weakens the i

70 replicase interaction requires a positively charged residue at the third position (3R) in the N-term

71 o wt and E123D, indicating that a negatively charged residue at this position functions as an effecti

72 ng this mutant demonstrate that a negatively charged residue at this position is essential for normal

73 d binding in receptors carrying a positively charged residue at this position.

74 lity appeared to require a second positively charged residue at VP1-109.

75 Furthermore, the introduction of positively charged residues at position 345 rendered shifted biphas

76 tivity have been generated by elimination of charged residues at the A1-A2 and A2-A3 interfaces.

77 The presence of a pair of oppositely charged residues at the aforementioned positions in beta

78 identified the importance of four positively charged residues at the base of the cysteine-rich head a

79 sitively charged patch to bind to negatively charged residues at the C-termini of P-proteins.

80 o membrane-protein insertion from positively charged residues at the cytoplasm-membrane interface and

81 Notably, the close proximity of charged residues at the dimer interface is a major deter

82 Previously, charged residues at the edge of the LPS binding pocket h

83 Here, we determined the role of the charged residues at the end of TM-1 in voltage sensing i

84 n the amino acid sequence, we also show that charged residues at the fibril three-fold apices provide

85 ggregate, including enrichment of gatekeeper charged residues at the flanks of hydrophobic aggregatio

86 e analysis of the photoadducts revealed that charged residues at the N-terminus of the signal sequenc

87 Mutations of charged residues at the rim of Hfq interfered with posit

88 Exposure of hydrophobic (respectively charged) residues can lead to disk (respectively ring) d

89 The present study evaluated the role of charged residue clusters in the regulation of MMP collag

90 cted at a limited subset of highly conserved charged residues, combined with ODN screening to elimina

91 ly located on the sequence, these positively charged residues concentrate in the tertiary structure a

92 although electrostatic interactions between charged residues contribute significantly to the overall

93 eport, we demonstrate that distal positively charged residues contribute to substrate binding in a sy

94 LSus of adjacent L2 dimers, where negatively charged residues coordinate around a Mg(2+) ion in a fas

95 model, confirmed that each of the positively charged residues critical for sweetness is close to a re

96 This study confirmed the importance of the charged residues D219 and E220 in maintaining structural

97 ppressor analysis suggests that one of these charged residues, D87, has distal influence on interhexa

98 he presence of neutral instead of positively charged residues did not interfere with POMP10 localizat

99 The fraction of charged residues discriminates between weak and strong p

100 arity of the sulfate moieties on the GAG and charged residues displayed on the fibril surface.

101 h subunit of the channel has four negatively charged residues distributed in the transmembrane segmen

102 e, from the (19)F-NMR analysis two important charged residues, E7 and R28, were found to be positione

103 ing three pairs of positively and negatively charged residues (either Glu(-)/Arg(+), Asp(-)/Arg(+), o

104 e majority of the binding free energy, while charged residues elsewhere are less critical for binding

105 the channel, comprised of opposing rings of charged residues, enforces directionality by interacting

106 These positively charged residues establish the correct N(exo)/C(cyt) top

107 It is clear that charged residues exert significant influence over struct

108 anged to a four-TMD molecule by mutating the charged residues flanking the first TMD.

109 d the importance of the conserved positively charged residue for the function of the Escherichia coli

110 packing interactions, whereas the positively charged residues form an exterior polar shell.

111 rge zipper with interdigitated complementary charged residues from Hha and the two units of the H-NS

112 Elimination of all negatively charged residues from the upper vestibule had no effect

113 ains a cluster of three conserved negatively charged residues Glu-179, Asp-180, and Asp-181 that coul

114 ctrostatic and dipolar effects caused by the charged residues (Glu113, Glu181) and to strong hydrogen

115 n signaling motif, but there is a negatively charged residue (glutamic acid) within the transmembrane

116 Mutagenesis of Cys-rich domain-charged residues had no major effect on ADAMTS13 functio

117 dioxygen, demonstrating that the positively charged residue (His548) plays a significant role in cat

118 mily members contain a cluster of positively charged residues (i.e. a "polybasic domain"), directly p

119 helmingly located in the vicinity of E929, a charged residue in a hydrophobic position of the heptad

120 We examine the role of Lys-377, the only charged residue in helix XI, on the functional mechanism

121 Each of these DDR events requires a charged residue in RNF168 (R57).

122 d predominantly to those having a negatively charged residue in the -2 position relative to the aspar

123 dies establish why mutations in a positively charged residue in the cationic pocket of an activation

124 The rationale was that a charged residue in the drug-binding pocket would disrupt

125 The sole negatively charged residue in the first half of human tropoelastin

126 Two mutations affecting the same positively charged residue in the S4 domain of K(V)7.2 have been fo

127 el family, TRPV5 and TRPV6 lack a positively charged residue in the TM4-TM5 loop that was shown to in

128 Tom1 recognizes specific, positively charged residues in a Dia2 degradation/NLS domain.

129 constrained refitting of residue charges for charged residues in Amber ff99SB( *) significantly impro

130 complex indicated that vCCI uses negatively charged residues in beta-sheet II to interact with posit

131 Replacing hydrophobic amino acids with charged residues in beta-strands 1, 2, and 19 interfered

132 tic and biochemical approaches, we show that charged residues in Csm3 facilitate its self-assembly an

133 -1 further confirm the critical roles of the charged residues in eotaxin-1.

134 rminus in the cytosol, and mutation of these charged residues in hGAAP ablated its anti-apoptotic fun

135 d with 87 residues in HP1b) is critical; the charged residues in HP1a are necessary for tight peptide

136 y associated with the presence of positively charged residues in its transmembrane (TM) region.

137 completed the functional analysis of all 57 charged residues in MurJ and demonstrated that the respe

138 g arginines in the S4 segment and negatively charged residues in neighboring transmembrane segments.

139 ic residues in subsite S2 and for positively charged residues in S1.

140 lysis, we find that the conserved positively charged residues in S4 are stabilized by countercharges

141 Either positively or negatively charged residues in that position completely blocked GCA

142 In addition, hydrophobic and charged residues in the 104 through 125 segment, arrange

143 luster (by nitrosylation) permits positively charged residues in the C-terminal helix to engage in DN

144 From these data, we hypothesize that some charged residues in the cavity region of MurJ homologs a

145 Previous studies showed that 8 charged residues in the central cavity region of MurJ ar

146 SPICE and VCP is the presence of oppositely charged residues in the central complement control modul

147 Mutation of conserved charged residues in the deltaretrovirus bovine leukemia

148 ing the cell cycle by recognizing positively charged residues in the Dia2 degradation/NLS domain and

149 Four charged residues in the fifth cytoplasmic loop (CL5) con

150 also found that, upon neutralization of the charged residues in the first turn of M2, the control of

151 ype Pin1 WW domain, which has two positively charged residues in the first turn, was compared to the

152 We have now defined the contributions of charged residues in the FL region of the Rous sarcoma vi

153 ltage-sensor domains (VSDs) where positively charged residues in the fourth transmembrane segment (S4

154 Next, we identified multiple positively charged residues in the inner ring of HUS1 that were cru

155 , suggesting the involvement of two critical charged residues in the interaction of fVIII with LRP1.

156 hannel opening, and is enabled by negatively charged residues in the linker region.

157 demonstrates the requirement for negatively charged residues in the loop regions for divalent ion bi

158 in beta-sheet II to interact with positively charged residues in the MIP-1beta N terminus, 20s region

159 Along with hydrophobic residues, charged residues in the N terminus of cdE2 were critical

160 and likely interacts with several positively charged residues in the N-lobe of Ca(2+)-CIB1.

161 of NCp7 mutants, the presence of positively charged residues in the N-terminus was found to be essen

162 single histidine (H) substitutions of these charged residues in the Na(v)1.4 channel to probe the po

163 s and shown to be comprised of complementary charged residues in the NCAM Ig2 domain (Arg-156 and Lys

164 ces in the number and position of positively-charged residues in the outer sides of the junction bind

165 es, the distance and order of the oppositely charged residues in the peptide sequence differ, such th

166 arged amino acids with neutral or negatively charged residues in the receptor-binding region of apoE

167 However, the more positively charged residues in the region facilitate DNA binding, l

168 rine-threonine-rich sequence with positively charged residues in the RelA NTD.

169 rated without altering the invariant ring of charged residues in the selectivity filter that governs

170 and R17 is the non-native burial of the two charged residues in the transition state.

171 This suggests that charged residues in the translocated domain and the hydr

172 s, revealing that ionic interactions between charged residues in the transmembrane domains of RNF170

173 observed and correlated with an asymmetry in charged residues in the vicinity of the inner and outer

174 Two negatively charged residues in these networks are putative Ca(2+) b

175 anslocated peptide region is lowered and the charged residues in this region are removed, translocati

176 Mutating charged residues in this region of Spt5 did not prevent

177 Abeta oligomers is influenced by positively charged residues in two sites (positions 23-31 and 95-10

178 ria, we explored the conservation of these 8 charged residues in YtgP, a homolog from Streptococcus p

179 analysis and transport assays indicate that charged residues, in addition to the methionine pairs an

180 hods, we observe that accessory mutations of charged residues increase protein stability, playing a k

181 nsistent with the suggestion that positively charged residues interact with the negatively charged ri

182 oop and coincides with a patch of positively charged residues involving arginines 102, 104, 106, and

183 tein studies, we confirmed that a positively charged residue is a SecYEG determinant for the endogeno

184 None of these positively charged residues is required for electron transfer, but

185 nd provide a unique insight into the role of charged residues K80, K277, R284, R285, and E388 at the

186 te group extends outward to draw in the four charged residues, leading to closure of beta13/beta14 to

187 Increased segregation of oppositely charged residues leads to linear decreases in the global

188 ontaining exposed hydrophobic and positively charged residues likely involved with membrane binding.

189 ocked on the bilayer, the interactions among charged residues, lipid bilayer, and calcium ions are op

190 nctional studies demonstrating that specific charged residues localized in the central cavity are ess

191 bearing alanine substitutions for positively charged residues located at positions 5, 7, 10 and 11 ar

192 the putative binding pocket (defined by four charged residues located in beta1, beta13 and beta14) in

193 wn to be governed by a cluster of positively charged residues located in its N-terminal segment.

194 ata obtained showed that mutations affecting charged residues located in the more distal portion of S

195 The positively charged residues located in the RNA-binding crevice play

196 Instead, we observe that substitutions of charged residues located in the TatA amphipathic helix l

197 at sequentially neutralized three positively charged residues (Lys-49, Lys-53, and Arg-57) within the

198 , 61 and 62) in the beta-turn and positively-charged residues (Lys72, Arg73, Arg74, Lys79 and Arg81)

199 The S4 segment contains several positively charged residues, mainly arginines, located at every thi

200 ter molecules in protein cavities containing charged residues may be subject to entropy changes that

201 condense on the protein ions formed via the charged residue mechanism.

202 SI for these folded species proceeds via the charged residue mechanism.

203 the ion evaporation model (IEM), whereas the charged residue model (CRM) applies to large globular sp

204 Our results confirm the Rayleigh/charged residue model (CRM).

205 evaporation to dryness, as envisioned by the charged residue model.

206 ty, these results cannot be explained by the charged-residue model alone.

207 The charged-residue model is most often used to explain the

208 ructure contains a large patch of positively charged residues, most of which are evolutionarily conse

209 Of five negatively charged residues mutated, none completely eliminated cha

210 Of five positively charged residues mutated, only one inactivated the chann

211 nity block, and that substituting negatively charged residues (N171D, N171E) at this position dramati

212 main are not due solely to interactions with charged residues near phosphorylatable serines and provi

213 These peptide structures commonly positioned charged residues near the membrane interface to promote

214 zyme and directly by contributing positively charged residues near the RNA-binding cleft.

215 nteractions and is allosterically coupled to charged residues near the site of SWCNT attachment.

216 R24A,R25A)-cystatin C, with substitutions of charged residues not involved in enzyme inhibition, was

217 s, we identified a subset of hydrophobic and charged residues of CC MBS (localized within and adjacen

218 Two negatively charged residues of KCNE3 (D54 and D55) are found essent

219 Positively and negatively charged residues of ribosomal proteins tend to be cluste

220 cherichia coli to re-assess the roles of the charged residues of TatA.

221 DAMGO)-related glycopeptides by altering the charged residues of the amphipathic helical address were

222 region of the N-Cap interact with negatively charged residues of the C-linker domain.

223 ibility and the reduced number of negatively charged residues of the D219/E220 deletion mutant, we me

224 atic interaction between E220 and positively charged residues of the linker in TIS11d.

225 4 PMCA is disturbed by changes of negatively charged residues of the N-terminal region.

226 bc9 residues interacting with the negatively charged residues of the NDSM.

227 ioleoyl-sn-glycero-3-phosphocholine bilayer, charged residues of the protein are trapped in the hydro

228 n of the lysine between two other positively charged residues of the Rossmann fold.

229 in place by the interactions with positively charged residues of VP1.

230 A constellation of charged residues on and around the arginine-rich helix o

231 ke domains results in clusters of positively-charged residues on each becoming arranged to form a con

232 re we have experimentally tested the role of charged residues on stability and folding kinetics of on

233 In addition, we reveal a cluster of charged residues on the Bre1 RING domain that is critica

234 ed a new interaction between complementarily charged residues on the cpFtsY G-domain and the vicinity

235 Mutation of several conserved and positively charged residues on the exterior surface of EcDnaB resul

236 Charged residues on the LBD surface form pathways that f

237 e high-occupancy sites in close proximity to charged residues on the protein surface.

238 the energy of insertion of TRPV1 by exposing charged residues on the S4 segment to solution.

239 its surface, is attracted by the negatively charged residues on the vestibular wall and the selectiv

240 g face using NMR and then mutated positively charged residues on this surface with a series of 16 Ala

241 f electrostatic interactions with negatively charged residues on troponin C.

242 when D231 and K178 were replaced with larger charged residues or when their positions were exchanged.

243 in an assembled state, suggesting that these charged residues play a critical role in the protein tra

244 However, two charged residues present in the modelled interface with

245 In addition, our results indicate that charged residues proximal to the seven vestibular portal

246 binding site, including only two positively charged residues (R122 and K141) positioned precisely in

247 The deleted fragment contains the positively charged residues R198 and K201, adjacent to layers 7 and

248 Mutation of a positively charged residue (R233Q in rat) showed that Ca2+-dependen

249 In addition, mutation of positively charged residues required for lipid binding disrupted VA

250 We identified a network of mainly charged residue-residue interactions spanning from the P

251 tin-3, but further mutagenesis of negatively charged residues revealed additional structural componen

252 nserved, the linear patterning of oppositely charged residues shows minimal variation.

253 ency toward both enzymes, whereas negatively charged residues significantly reduced it.

254 U.1909 and SMU.925, which lack the last four charged residues (SKNK) that are present in SMU.152 but

255 N-glycosylation efficiency, while positively charged residues such as Arg suppressed N-glycosylation.

256 through several highly conserved positively charged residues such as K752 and K619 to release from t

257 be achieved by neutralizing several specific charged residues suggesting that they may play an active

258 Further increasing the polarity, by adding charged residues, switches the insertion pathway to a Yi

259 ugar transporters, we identify an additional charged residue that may be essential for effective H(+)

260 The electrostatic network includes charged residues that are pore-lining and thus positione

261 Here we examined the role of conserved charged residues that form electrostatic networks near t

262 ystematically analyze each of the negatively charged residues that mediate binding of Ca(2+) to the b

263 itution of one of a Gly-Gly pair with highly charged residues that significantly increase structural

264 ons between residue 327 and these negatively charged residues, thus favoring channel opening.

265 Strikingly, the addition of a positively charged residue to either the translocated region or the

266 s unclear to what extent CDRs can accumulate charged residues to increase antibody affinity without c

267 o protonate the leaving group and positively charged residues to stabilize the transition state, expl

268 reducing the thermodynamic cost of exposing charged residues to the hydrophobic core.

269 , also showed that mutating highly conserved charged residues to the oppositely charged amino acid ha

270 Changing either of two negatively charged residues to uncharged residues eliminates the ab

271 er salt bridges between the alphaC-helix and charged residues upstream of the NtA motif.

272 e determined by a combination of fraction of charged residues values and the linear sequence distribu

273 he linear sequence patterning of proline and charged residues vis-a-vis all other residues.

274 When this positively charged residue was introduced to either TRPV6 or TRPV5,

275 In contrast, the conserved positively charged residue was not required for the E. coli YidC or

276 n palmitoylation, provided that a positively charged residue was present within the first seven resid

277 the F(2) domain identified as being rich in charged residues was found to modulate fusion activity o

278 an equal number of positively and negatively charged residues, we found a striking correlation T( *)c

279 athic helices of all three peptides with the charged residues well exposed to the water phase.

280 Charged residues were poorly tolerated, conferring extre

281 removes the desolvation penalty paid by the charged residue, whereas the third introduces unanticipa

282 beta2e encompassing a cluster of positively charged residues, which is strictly required for membran

283 incompatibility between a pair of positively charged residues, which lie in close proximity to each o

284 d MMP-9 most greatly favored the presence of charged residues with preference for the Gly-Asp-Lys ser

285 transiently interacts through complementary charged residues with the FMN-binding site region of Ndo

286 ral pHLIP variants with different numbers of charged residues, with attached polar cargoes at the pep

287 a hydrophobic patch surrounded by positively charged residues, with subtle differences from other ICK

288 urans revealed that the conserved positively charged residue within transmembrane segment one (at pos

289 ct from DR1101 in its ability to accommodate charged residues within all but one of its binding pocke

290 essential in YtgP; YtgP possesses additional charged residues within its predicted cavity that are es

291 Segregation of oppositely charged residues within linear sequences leads to high k

292 A pair of positively charged residues within the amphipathic helix (the basic

293 Based on identified contacts, positively charged residues within the external waist region were m

294 terized viruses with mutations engineered at charged residues within the mu1 loop formed by residues

295 new consensus sequence, and that positively charged residues within the O-glucose consensus sequence

296 as strong ionic interactions with negatively charged residues within the S1-S3 helices in the resting

297 We identified several positively charged residues within these domains as having a crucia

298 ractions mediated by the abundant positively charged residues within these regions.

299 Substitution of the positively charged residues within this segment by alanine abolishe

300 Phe-1086 (in NBD2) with hydrophobic but not charged residues yielded active mutants.