ライフサイエンスコーパス: chemical cross-linking

1 ssembly of elastic fibers in vivo, preceding chemical cross-linking.

2 ated ELP3 and form stable helices only after chemical cross-linking.

3 ter exemplified by immunoprecipitation after chemical cross-linking.

4 of the CbbRRS system), confirmed in vitro by chemical cross-linking.

5 d to ssDNA was determined by protein-protein chemical cross-linking.

6 roduced using homology modeling coupled with chemical cross-linking.

7 inding interactions with SERCA2a measured by chemical cross-linking.

8 nsgenic mouse lens extracts prepared without chemical cross-linking.

9 luorescent protein to the plasma membrane or chemical cross-linking.

10 a2+ pump (SERCA2a) have been investigated by chemical cross-linking.

11 polymerase chain reaction (PCR), as well as chemical cross-linking.

12 stabilize the pulled nanotubes by subsequent chemical cross-linking.

13 ycol)-b-poly(L-lysine) (PEG-PLL) followed by chemical cross-linking.

14 ast to many chaperone complexes that require chemical cross-linking.

15 atography coupled with light scattering, and chemical cross-linking.

16 and alpha,gamma-associations as detected by chemical cross-linking.

17 lustering of alpha(v)beta(3) as confirmed by chemical cross-linking.

18 we investigated RGS7 complexes using in situ chemical cross-linking.

19 erize it by small-angle x-ray scattering and chemical cross-linking.

20 s (Ag-coupled splenocytes [Ag-SPs]) with the chemical cross-linking agent ethylene-carbodiimide, whic

21 We also used a chemical cross-linking agent to covalently cross-link PD

22 d a non-membrane-permeable cysteine-specific chemical cross-linking agent, a dimer is the predominant

23 results demonstrated that the application of chemical cross-linking agents to etched dentin prior to

24 oss-linking of the ecto-ATPase by lectin and chemical cross-linking agents.

25 ilization of peptide oligomers using in situ chemical cross-linking allowed detailed study of their p

26 tion of the proteins from DNA complexes, and chemical cross-linking analyses.

27 Gel filtration and chemical cross-linking analysis of the recombinant carbo

28 te the null-like drug sensitivity phenotype, chemical cross-linking analysis revealed no apparent def

29 Using gel filtration, chemical cross-linking, analytical ultracentrifugation,

30 Finally, chemical cross-linking, analytical ultracentrifugation,

31 longated dimers and tetramers as revealed by chemical cross-linking and analytical ultracentrifugatio

32 Using chemical cross-linking and co-immunoprecipitation of dif

33 Chemical cross-linking and coimmunoprecipitation show th

34 Here we have addressed this issue by both chemical cross-linking and direct imaging of individual

35 Chemical cross-linking and DNA sequencing have revealed

36 al significance has been verified using both chemical cross-linking and electron paramagnetic resonan

37 Here, we show using chemical cross-linking and fluorescence resonance energy

38 forms of each AUF1 protein variant, we used chemical cross-linking and gel filtration chromatography

39 Remarkably, gel shift, chemical cross-linking and gel filtration experiments sh

40 Chemical cross-linking and gel-filtration show that a 1:

41 omatography, analytical ultracentrifugation, chemical cross-linking and hydrodynamic modeling.

42 A combination of chemical cross-linking and hydrogen-deuterium exchange c

43 In the present work, chemical cross-linking and immunoprecipitation assays we

44 We applied Chemical Cross-Linking and Isolation by Pull Down (Chem-

45 Chemical Cross-Linking and Isolation by Pull-Down (Chem-

46 Chemical cross-linking and LC-MS/MS further indicated th

47 Chemical cross-linking and MALDI-TOF MS mapped these sam

48 By in vivo chemical cross-linking and MALDI-TOF-MS analysis we demo

49 alytic dimer (alphabeta), based primarily on chemical cross-linking and mass spectrometric analysis.

50 Using chemical cross-linking and mass spectrometry (XL-MS) com

51 ryo-electron microscopy and verified it with chemical cross-linking and mass spectrometry analysis.

52 Using a chemical cross-linking and mass spectrometry approach, w

53 Chemical cross-linking and mass spectrometry are now wid

54 raction but not for cleavage of Pro-sigma(K) Chemical cross-linking and mass spectrometry of purified

55 racterization of both transporters either by chemical cross-linking and mass spectrometry or by size-

56 We have performed chemical cross-linking and mass spectrometry, and combin

57 Here, chemical cross-linking and mass spectrometry, comparativ

58 Using a combination of chemical cross-linking and mass spectrometry, we find th

59 tagging-method, in combination with isotopic chemical cross-linking and mass spectrometry, we have lo

60 Using in vivo chemical cross-linking and mass spectrometry, we identif

61 Using chemical cross-linking and mass spectrometry, we identif

62 spholipid were deduced from a combination of chemical cross-linking and mass spectrometry.

63 s (rHDL) were attained from a combination of chemical cross-linking and mass spectrometry.

64 ein (HDL) was studied using a combination of chemical cross-linking and mass spectrometry.

65 analyzed by cryo-electron microscopy and by chemical cross-linking and mass spectrometry.

66 nic electron microscopy and a combination of chemical cross-linking and mass spectrometry.

67 Chemical cross-linking and molecular dynamics are consis

68 Based on the results of chemical cross-linking and molecular modeling, we propos

69 e C-type lectin-like domain of NKR-P1C using chemical cross-linking and molecular modeling.

70 to antigen-scaffold linkage: genetic fusion, chemical cross-linking and plug-and-display SpyTag/SpyCa

71 Chemical cross-linking and size exclusion chromatography

72 gions of Pluronic F127 micelles, followed by chemical cross-linking and subsequent removal of non-cro

73 Using a proteomic approach based on in-cell chemical cross-linking and tandem affinity purification

74 hermus aquaticus Ffh.FtsY complexes by using chemical cross-linking and tandem mass spectrometry to i

75 Using chemical cross-linking and tandem mass spectrometry, we

76 Using chemical cross-linking and the yeast two-hybrid system,

77 beta(4) subcomplex was observed through both chemical cross-linking and top-down MS of PhK.

78 Here, we have used NMR, chemical cross-linking, and analytical ultracentrifugati

79 , enzyme-linked immunosorbent assay (ELISA), chemical cross-linking, and ATPase activity) provided ev

80 rs, increased susceptibility of integrins to chemical cross-linking, and biochemical detection of lar

81 yphenylalanine (DOPA) for periodate-mediated chemical cross-linking, and biotin was conjugated to Lys

82 Gel filtration, chemical cross-linking, and co-immunoprecipitation exper

83 oscopy, small-angle X-ray scattering (SAXS), chemical cross-linking, and enzymatic assays to enlarge

84 nt techniques: small-angle X-ray scattering, chemical cross-linking, and enzyme kinetics.

85 Deletion analysis in vivo, chemical cross-linking, and manipulation of the ATP conc

86 y, bimolecular fluorescence complementation, chemical cross-linking, and reciprocal coimmunoprecipita

87 Fluorescence, chemical cross-linking, and saturation transfer differen

88 opy, X-ray crystallography, residue-specific chemical cross-linking, and several proteomics technique

89 erium exchange coupled to mass spectrometry, chemical cross-linking, and small angle x-ray scattering

90 e results of analytical ultracentrifugation, chemical cross-linking, and tryptophan fluorescence anal

91 We employed a chemical cross-linking approach to probe the structure a

92 We used chemical cross-linking approaches to map interdomain int

93 tron-microscopy, cysteine-accessibility, and chemical cross-linking, as well as by computational appr

94 Chemical cross-linking assays showed that the mutated Th

95 Gel shift, DNase I, and chemical cross-linking assays with TATA box-binding prot

96 The mechanism of chemical cross-linking at pH 7.4 and 37 degrees C was st

97 nts of the needle-translocon complex using a chemical cross-linking-based approach.

98 rium exchange (HDX), protein footprinting or chemical cross-linking can provide us with structural in

99 protein, which is readily demonstratable by chemical cross-linking, can be completely dissociated by

100 Chemical cross-linking combined with an enzymatic digest

101 Chemical cross-linking combined with immunoprecipitation

102 Chemical cross-linking combined with mass spectrometry (

103 receptor, as the receptor for TLQP-21 using chemical cross-linking combined with mass spectrometry a

104 is, electron microscopic reconstruction, and chemical cross-linking combined with mass spectrometry a

105 Chemical cross-linking combined with mass spectrometry c

106 Chemical cross-linking combined with mass spectrometry i

107 to investigate protein/protein interactions, chemical cross-linking combined with mass spectrometry r

108 In this study, we used chemical cross-linking combined with mass spectrometry t

109 ultiple experimental constraints provided by chemical cross-linking combined with mass spectrometry,

110 Chemical cross-linking combined with proteolytic digesti

111 Using chemical cross-linking, competition studies, and NMR che

112 In addition, chemical cross-linking conferred activity upon G85R, an

113 Photoreactive heterotrifunctional chemical cross-linking confirmed the interaction between

114 Analytical ultracentrifugation and chemical cross-linking confirmed the presence of dimers

115 Hydrogen/deuterium exchange and chemical cross-linking coupled to mass spectrometry reve

116 Chemical cross-linking coupled with mass spectrometric a

117 Chemical cross-linking coupled with mass spectrometry (C

118 We applied chemical cross-linking coupled with mass spectrometry an

119 Chemical cross-linking coupled with mass spectrometry pl

120 ral approach using cryo-electron microscopy, chemical cross-linking coupled with mass spectrometry, a

121 pts red cell membranes were elucidated using chemical cross-linking coupled with mass spectrometry.

122 combination of site-directed mutagenesis and chemical cross-linking, coupled with crystallographic an

123 des this nonspecific oligomerization, MS and chemical cross-linking data combined with CD spectra pro

124 Chemical cross-linking demonstrated that carbohydrates a

125 tic analysis, analytical gel filtration, and chemical cross-linking demonstrated that the nucleotide-

126 ally tagged RGS7 constructs, with or without chemical cross-linking, demonstrated RGS7 self-associati

127 -protein interface from NMR spectroscopy and chemical cross-linking detected by mass spectrometry.

128 Using chemical cross-linking, eight potential protein constitu

129 or-intensive biophysical experiments such as chemical cross-linking, electron paramagnetic resonance,

130 body specific to K-varepsilon-GG to beads by chemical cross-linking, enrichment of ubiquitinated pept

131 Chemical cross-linking experiments carried out previousl

132 rotein-protein interactions was supported by chemical cross-linking experiments combined with LC-MS/M

133 Chemical cross-linking experiments confirm that FAD2 and

134 ssociated with the tetramer as isolated, and chemical cross-linking experiments confirm that the tetr

135 Mutagenesis and chemical cross-linking experiments confirm the importanc

136 Formation of dimers was shown by chemical cross-linking experiments for interactions of T

137 itation, analytical ultracentrifugation, and chemical cross-linking experiments have shown that an in

138 Sedimentation equilibrium and chemical cross-linking experiments performed at increasi

139 Cell adhesion and chemical cross-linking experiments revealed that oligome

140 ative polyacrylamide gel electrophoresis and chemical cross-linking experiments suggested that AcrB(D

141 Chemical cross-linking experiments using GTP-gamma-azido

142 e cross-linking reagents and methodology for chemical cross-linking experiments using tandem mass spe

143 Chemical cross-linking experiments were also performed a

144 Chemical cross-linking experiments with biotinylated ann

145 Chemical cross-linking experiments, perfluoro-octanoate-

146 and optional restraints from proteomics and chemical cross-linking experiments.

147 major bottleneck for mass spectrometry based chemical cross-linking experiments.

148 membrane-based yeast two-hybrid system, and chemical cross-linking experiments.

149 scopy, transmission electron microscopy, and chemical cross-linking experiments.

150 This finding was confirmed by chemical cross-linking experiments.

151 sed to unravel protein interaction surfaces, chemical cross-linking followed by identification of the

152 Here, we report chemical cross-linking followed by immunodetection and l

153 To address this, we have utilized chemical cross-linking followed by mass spectrometry and

154 Chemical cross-linking followed by mass spectrometry yie

155 Chemical cross-linking followed by micrometric flow cyto

156 tudy of proteins and protein complexes using chemical cross-linking followed by the MS identification

157 scopy along with our established paradigm of chemical cross-linking followed by tryptic digestion, ma

158 oligomerization has mainly been assessed by chemical cross-linking following cell fractionation stud

159 tionships are investigated: (i) differential chemical cross-linking for the control of membrane disas

160 ucture of hOAT1 using combined approaches of chemical cross-linking, gel filtration chromatography, c

161 nd immunoprecipitations that use physical or chemical cross-linking--have been developed to address t

162 By employing chemical cross-linking, high proton conductivities can b

163 This procedure utilized chemical cross-linking, hydrogen/deuterium exchange, and

164 Chemical cross-linking in combination with mass spectrom

165 Chemical cross-linking in combination with solution-stat

166 We now use chemical cross-linking in synaptosomes, pinched-off nerv

167 osed on the basis of the analytical data and chemical cross-linking in tandem with mass analysis usin

168 s and multimers observed, but performing the chemical cross-linking in the presence of a reducing age

169 Chemical cross-linking in vivo and copurification approa

170 Here we show that chemical cross-linking increases trimer stability, reduc

171 gel electrophoresis, mass spectrometry, and chemical cross-linking indicated that NanR forms homodim

172 Chemical cross-linking indicates that full-length NS3 fo

173 at least two PC bound that is stabilized by chemical cross-linking interacts more effectively with a

174 Chemical cross-linking is an attractive approach to map

175 Chemical cross-linking is employed here to probe differe

176 In addition, via chemical cross-linking, limited proteolysis, and mass sp

177 rstanding of mitochondrial function, we used chemical cross-linking mass spectrometry to identify 2,4

178 wo well established experimental approaches: chemical cross-linking/mass spectrometry (MS) and intern

179 The number of publications in the field of chemical cross-linking/mass spectrometry (MS) for derivi

180 this end, we have utilized a combination of chemical cross-linking/mass spectrometry and computation

181 Here, we used a chemical cross-linking method together with molecular dy

182 ere prepared as Fab' x Fab' conjugates using chemical cross-linking methods and as bispecific diabodi

183 lized near the actin and myosin interface by chemical cross-linking methods, but its functional contr

184 S, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modeling restraints to co

185 he entire beta subunit was constructed using chemical cross-linking, MS, threading, and ab initio app

186 trophysiology, chemical ligand modification, chemical cross-linking, MS/MS-analyses and molecular mod

187 s on the structural information derived from chemical cross-linking/MS experiments.

188 e oligomeric structure of Ano1, we performed chemical cross-linking, non-denaturing PAGE, and electro

189 Chemical cross-linking of acidic residues is achieved us

190 Chemical cross-linking of detergent-resistant membranes

191 Chemical cross-linking of ferritin at 12 A spacing led t

192 nteracting residues on FXIII-A(2)* following chemical cross-linking of fibrin(ogen) alphaC389-402 pep

193 Chemical cross-linking of focal adhesion preparations wi

194 Chemical cross-linking of GG and mutagenesis of full-len

195 We anticipated that chemical cross-linking of HIV-1 would allow purification

196 We used site-directed chemical cross-linking of Int in trapped Holliday juncti

197 Chemical cross-linking of intact membrane proteins from

198 In this study, we used chemical cross-linking of introduced cysteine pairs in a

199 Chemical cross-linking of maize leaves followed by immun

200 Bifunctional chemical cross-linking of MENT revealed oligomerization

201 The use of mass spectrometry coupled with chemical cross-linking of proteins has become a powerful

202 Chemical cross-linking of proteins in combination with m

203 Animal studies of chemical cross-linking of sclera as a potential treatmen

204 fulfills distance constraints obtained from chemical cross-linking of the complex at multiple recurr

205 Chemical cross-linking of the complex B core resulted in

206 Chemical cross-linking of the complex followed by trypti

207 y adsorbed on Au microelectrodes followed by chemical cross-linking of the enzymes acetylcholinestera

208 Static light scattering and chemical cross-linking of the three RFX proteins show th

209 Here, we used chemical cross-linking of topo IV to demonstrate that en

210 By contrasting chemical cross-linking of untreated and dephosphorylated

211 locked in either the closed or open state by chemical cross-linking or deletion mutagenesis and showe

212 Chemical cross-linking or interaction with hsp70 increas

213 t amounts of stable APD in solution, without chemical cross-linking or polymerization-affecting compo

214 erences in proteolytic cleavage patterns and chemical cross-linking patterns were consistent with kno

215 oaches, including site-directed mutagenesis, chemical cross-linking, peptide mapping, and LC-MS/MS an

216 We used a combination of chemical cross-linking, proteolytic digestion, and mass

217 We used homo- and hetero-bifunctional chemical cross-linking reagents, BS3 and sulfo-SMPB, res

218 loops in Drosophila were determined by Hi-C (chemical cross-linking, restriction digestion, ligation,

219 dynamic neuron-oligodendrocyte signaling by chemical cross-linking results in aberrant myelination o

220 Chemical cross-linking results support the IFT52-IFT88 i

221 Site-directed mutagenesis combined with chemical cross-linking revealed that residue 238 of DrAB

222 Chemical cross-linking revealed that the major soluble f

223 gle x-ray scattering (SAXS) measurements and chemical cross-linking revealed that the pASK1-CD.14-3-3

224 red to as the "pointed-end complex." We used chemical cross-linking, RNA interference, and protein ov

225 d immunosorbent assay, Western blot, in vivo chemical cross-linking, ROS assay, and immunofluorescenc

226 However, chemical cross-linking showed that 7B2 exhibits concentr

227 electrophoresis under anoxic conditions and chemical cross-linking showed that holo-NfuA forms dimer

228 Chemical cross-linking showed that LGN neuron preapoptos

229 Gel filtration and chemical cross-linking showed that the complex exists as

230 Chemical cross-linking shows that CggR oligomerization i

231 Chemical cross-linking shows that pathogenic DJ-1 mutant

232 in the monomeric state (for example, through chemical cross-linking) significantly hampers the fibril

233 lved in the Fd-Delta9D complex by the use of chemical cross-linking, site-directed mutagenesis, stead

234 Using mutagenesis, chemical cross-linking, size exclusion chromatography, a

235 tathione S-transferase pulldown experiments, chemical cross-linking, size exclusion chromatography, c

236 on method involves microsomal fractionation, chemical cross-linking, solubilization, and one-step aff

237 upted by solubilization in Triton X-100, but chemical cross-linking stabilizes a putative assembly in

238 allenges in protein interaction studies with chemical cross-linking stems from the complexity of intr

239 hylaminopropyl) carbodiimide (EDC)-mediated, chemical cross-linking step that enhances detection of s

240 e G protein-coupled receptor-Galphai protein chemical cross-linking strategy to map the cannabinoid r

241 Using a chemical cross-linking strategy, complexes of AlkB-doubl

242 NA and ABH2-dsDNA complexes, stabilized by a chemical cross-linking strategy.

243 e basis for small angle x-ray scattering and chemical cross-linking structural analysis of the discre

244 Chemical cross-linking studies indicate an inverse corre

245 ociate, and gel-exclusion chromatography and chemical cross-linking studies indicated that atlastin-1

246 s, density-gradient ultracentrifugation, and chemical cross-linking studies indicated that the functi

247 Co-immunoprecipitation and chemical cross-linking studies previously revealed that

248 Chemical cross-linking studies revealed SAMHD1 tetramers

249 Chemical cross-linking studies revealed specific sites o

250 or for XMRV, Xpr1, mediates this effect, and chemical cross-linking studies show that Xpr1 is associa

251 P3, binding analyses, reaction kinetics, and chemical cross-linking studies were used to demonstrate

252 Here we employ chemical cross-linking studies, a novel co-immunoprecipi

253 are confirmed by independent mutagenesis and chemical cross-linking studies.

254 ystems, immunoprecipitation experiments, and chemical cross-linking studies.

255 homodimer." Furthermore, the EPR data and a chemical cross-linking study demonstrated the existence

256 We combined mass spectrometry (MS) with chemical cross-linking, surface accessibility measuremen

257 Although chemical cross-linking takes longer (15 min to 2 h) than

258 confirm these split-ubiquitin findings by a chemical cross-linking technique.

259 Using novel label transfer and chemical cross-linking techniques, we show that ubiquiti

260 been evaluated by SDSL EPR spectroscopy and chemical cross-linking techniques.

261 Consistent with this, we identified through chemical cross-linking that Ecm29 binds to, or in close

262 ss, we show by a yeast two-hybrid system and chemical cross-linking that the lumenal domain of IRAP c

263 ed every prediction tested: Any mutation (or chemical cross-linking) that impaired a conformational r

264 By chemical cross-linking the C domain interacts with the p

265 Chemical cross-linking, thermal denaturation, and size f

266 Instead of ultraviolet or chemical cross-linking, this method utilizes natural hyd

267 angle x-ray scattering and isotope-assisted chemical cross-linking to apoA-I(Delta185-243) in its di

268 To build a more complete model, we used chemical cross-linking to derive distance constraints ac

269 e used time-resolved limited proteolysis and chemical cross-linking to examine nucleotide-induced str

270 , electron paramagnetic resonance (EPR), and chemical cross-linking to probe for intermolecular inter

271 y we used quantitative mass spectrometry and chemical cross-linking to quantify differences in cross-

272 Ono et al. used rapid, zero-length, in situ chemical cross-linking to stabilize the oligomer state,

273 ested this by using cysteine mutagenesis and chemical cross-linking to verify proximal relationships

274 Chemical cross-linking together with immunoelectron micr

275 Chemical cross-linking, together with mass spectrometry

276 By chemical cross-linking, transiently expressed hRFC in hR

277 The KD from chemical cross-linking was 12.7 +/- 1.1 microM, and from

278 und no preferential dimer formation, whether chemical cross-linking was performed in the presence or

279 Chemical cross-linking was used to study protein binding

280 Using chemical cross-linking we have previously reported that

281 Using protein docking prediction and chemical cross-linking, we demonstrate that EIIA(Glc) bi

282 Using chemical cross-linking, we demonstrated that CFTR exists

283 Although fluidity is suppressed by chemical cross-linking, we find that ATP depletion in th

284 ion and complex structure associations using chemical cross-linking, we have developed a combination

285 Using NMR spectroscopy, gel filtration, and chemical cross-linking, we obtained direct evidence for

286 dy, using analytical ultracentrifugation and chemical cross-linking, we show that calcium or strontiu

287 Using chemical cross-linking, we show that the MDM2 RING domai

288 Further, by using chemical cross-linking, we showed that the chimeric prot

289 this protein to form dimers, as detected by chemical cross-linking, were consistent with the higher

290 ected mutagenesis, cysteine replacement, and chemical cross-linking, were employed to identify contac

291 ini-spectrin dimer were probed further using chemical cross-linking, which identified distinct groups

292 arrier- and PCFT-null HeLa (R1-11) cells and chemical cross-linking with 1,1-methanediyl bismethaneth

293 Thermal curing under reduced pressure or chemical cross-linking with a diepoxide was shown to fix

294 L-6 antibody and protein G was stabilized by chemical cross-linking with glutaraldehyde and the captu

295 After chemical cross-linking with glutaraldehyde in the presen

296 Large-scale chemical cross-linking with mass spectrometry (XL-MS) an

297 Chemical cross-linking with mass spectrometry (XL-MS) pr

298 termed "multimer-PAGE," that combines in-gel chemical cross-linking with several common electrophoret

299 lose sulfhydryl groups in proteins employing chemical cross-linking with the fluorogenic, homobifunct

300 cted mutagenesis, fluorescence labeling, and chemical cross-linking, with yeast actin mutants contain