ライフサイエンスコーパス: chemically-defined

1 media, attachment surfaces and splitting are chemically defined.

2 Glucan phosphate, a water-soluble, chemically defined (1-->3)-beta-D-glucan biologic respon

3 ost-implantation mouse and rat embryos using chemically defined, activin-containing culture medium th

4 o allows high-yield (>250%) MN production in chemically defined adherent cultures.

5 e that these materials may also be useful as chemically defined adjuvants.

6 V often received excitatory inputs from two chemically defined afferent pathways.

7 yclodextrin represents the first transparent chemically defined agar and the first urease indicator a

8 We now show that the addition of a chemically defined AGE (methyl-glyoxal-BSA) to low-AGE m

9 These chemically defined alternatives to currently used, undef

10 The ability to generate chemically defined analogues of GPI-anchored proteins is

11 Furthermore, the observed channels are chemically defined and have diameters related to oligome

12 m self-renewal of hES cells under completely chemically defined and xeno-free conditions without ROCK

13 A chemically defined anti-CXCR4-auristatin antibody-drug c

14 These experiments support the notion that chemically defined antibody conjugates can be synthesize

15 The resulting chemically defined antibody-drug conjugates represent th

16 These chemically defined antigens identified the M antigenic d

17 mer-based therapeutics and suggest that such chemically defined APCs may afford a useful targeted del

18 We demonstrate that H. pylori grows in the chemically defined broth medium Ham's F-12 nutrient mixt

19 Chemically defined (CD) and hydrolysate-supplemented med

20 proven important for producing homogeneous, chemically defined complexes for structural studies.

21 specific infectivity have been produced from chemically defined components in vitro.

22 The chemically defined components of the bacterial envelope

23 CM) to a non-covalent structure with minimal chemically defined components represents an attractive a

24 Using sulfated versus non-sulfated forms of chemically defined compounds in binding assays employing

25 primitive neuroepithelium within 1 wk under chemically defined condition.

26 an undifferentiated, pluripotent state under chemically defined conditions in the absence of feeder c

27 Using hiPSCs derived under chemically defined conditions on synthetic matrices, we

28 neural progenitor cells (NPCs) maintained in chemically defined conditions to study HCMV replication

29 oculture with other cell types and relies on chemically defined conditions up to and including the ge

30 ach for the large-scale generation of MKs in chemically defined conditions using a forward programmin

31 Under chemically defined conditions, the galactofuranosyltrans

32 ted extensively for more than 18 passages in chemically defined conditions, with 10(5) starting fibro

33 Cellular reprogramming using chemically defined conditions, without genetic manipulat

34 e a novel 3-step differentiation protocol in chemically defined conditions.

35 d populations of preOLs and mature OLs under chemically defined conditions.

36 observed in retinal explants cultured under chemically defined conditions.

37 o distinct populations of SMC subtypes under chemically defined conditions.

38 d from mouse embryonic stem (ES) cells under chemically defined conditions.

39 level of 3% (previously termed hypoxia) and chemically defined conditions.

40 thod suitable for the routine preparation of chemically defined conjugates for in vivo applications.

41 cells (ESCs) or induced PSCs (iPSCs), under chemically defined culture conditions, with ex vivo cult

42 We demonstrate that the chemically-defined cysteine-engineered antibody-tubulysi

43 Here, we use chemically defined "designer" nucleosomes to investigate

44 into proteins and had the same lifespan on a chemically defined diet with or without selenium supplem

45 In this report, a chemically-defined differentiation regimen and a monolay

46 Previous studies indicated that a chemically-defined, differentiation medium (DM) induces

47 We have synthesized a chemically defined, dimeric form of an erythropoietin mi

48 The chemically defined disulfide connectivity of the recombi

49 We devised a reconstituted system in which chemically defined donor membranes function to transfer

50 TA-based passaging procedure to be used with chemically defined E8 medium that serves as a tool for b

51 We report a chemically defined, efficient, scalable and reproducible

52 intravenous TPN, partially preserved with a chemically defined enteral diet, and completely preserve

53 We evaluated the effect of chemically defined extracts from Echinacea angustifolia

54 A chemically defined food (CDF) permits the systematic eva

55 uded organic acids, observed particularly in chemically defined formulations, and furans, pyridines,

56 owth in human saliva compared to growth in a chemically defined glucose-containing medium or a nutrie

57 ese interactions rely on the availability of chemically defined glycoconjugates that can be selective

58 uated the addition of these two nutrients to chemically defined grape juice and filter clarified Char

59 to synthesize arginine was assessed using a chemically defined growth medium.

60 s from pluripotent stem cells (PSCs) using a chemically defined, growth factor-rich medium.

61 Here we demonstrate that chemically defined guanidine 5'-diphosphate-beta-l-fucos

62 Hepatocytes were grown in chemically defined hepatocyte growth medium (HGM) contai

63 There is currently no complete chemically defined (holidic) diet available for Drosophi

64 as a new, user-friendly route for supplying chemically defined human glycans simply by combining bio

65 using a novel rapid protocol which uses only chemically-defined human-derived media additives and sub

66 emethylation, we subjected mouse ES cells to chemically defined hypomethylating culture conditions.

67 This approach based on fully synthetic chemically defined immunogens should be generally useful

68 activates DCs through TLR11 and is the first chemically defined ligand for this TLR.

69 The solutions were chemically defined lipid concentrate (CDLC) and model bi

70 supplements (Cholesterol Lipid Concentrate, Chemically Defined Lipid Concentrate, Lipid Mixture 1, G

71 m large ( approximately 300 nm in diameter), chemically defined liposomes incubated with coatomer, Ar

72 ut-PE) was synthesized and incorporated into chemically defined liposomes to provide binding sites fo

73 Thus, the incorporation of chemically defined LN-511-E8 into biosynthetic scaffolds

74 eisseria meningitidis strains that expresses chemically defined LOS components to determine the struc

75 hanol-fed rats recognized mainly a specific, chemically defined MAA epitope.

76 cost, robust, scalable culture methods using chemically defined materials need to be developed to add

77 e methods that are robust, scalable, and use chemically defined materials.

78 Here we show that a chemically defined maturation medium supplemented with t

79 he growth of the Deltasse strain in rich and chemically defined media and nonimmune human blood and s

80 The data also suggest that chemically defined media described herein could yield th

81 onicus were surveyed by employing culture in chemically defined media devoid of peptide nutrients and

82 re matrices such as biomimetic scaffolds and chemically defined media supplemented with essential nut

83 sing substrates of known matrix proteins and chemically defined media supplemented with exogenous gro

84 The approach uses chemically defined media under feeder-free conditions, a

85 bilities of M1 and M4 Mga mutants to grow in chemically defined media with a single sugar source comp

86 of equibactin synthesis, supplementation of chemically defined media with salicylate was required fo

87 ollagen gel cylinders that were incubated in chemically defined media with stromal-derived factor-1al

88 ost pronounced when S. aureus is cultured in chemically defined media with thiosulfate (TS) as a sole

89 ith the advent of new initiatives to develop chemically defined media, cell culture scientists screen

90 Colonies grown on two chemically defined media, including the one in which BMP

91 cultivation and recovery of H. pylori using chemically defined media.

92 elf-renewing neural crest-like stem cells in chemically defined media.

93 gated in serum (2-3 h) and differentiated in chemically defined medium (3-4 h).

94 tococcal proteases were recovered from spent chemically defined medium (CDM) and fractionated by ammo

95 Chemically defined medium (CDM) conditions for controlli

96 evelopment of competence was examined in the chemically defined medium (CDM) described by van de Rijn

97 ters were upregulated in vitro when grown in chemically defined medium (CDM) lacking BCAA.

98 The mtaR mutant grew poorly in chemically defined medium (CDM) prepared with methionine

99 cally, when aerobically grown in a low-iron, chemically defined medium (CDM), L. pneumophila secretes

100 was used to develop in silico and in vitro a chemically defined medium (CDM), which was validated exp

101 40 to 70% less CAS reactivity in deferrated chemically defined medium (CDM).

102 or alpha (TGFalpha) in the presence of a new chemically defined medium (HGM).

103 e sequence TVKHRPDALHPQ or LTTAPKLPKVTR in a chemically defined medium (mTeSR), they expressed marker

104 ar matrix coating, and either conditioned or chemically defined medium (mTeSR).

105 -Hewitt supplemented with excess glucose and chemically defined medium allowed survival for less than

106 ed in dimethyl sulfoxide (DMSO)-supplemented chemically defined medium are an excellent model system

107 When grown in chemically defined medium at an alkaline pH, strain 86-0

108 ively) were grown in continuous culture in a chemically defined medium at fast mass doubling time (t(

109 Oral viridans group streptococci, growing in chemically defined medium at pH 6.8, released HlpA into

110 ila was reduced approximately in half in the chemically defined medium compared with the same medium

111 ed cardiac differentiation strategy, using a chemically defined medium consisting of just three compo

112 s phenotypic abnormalities whereas growth in chemically defined medium containing 5 mM KCl more close

113 dc25A-rescued cells could not proliferate in chemically defined medium containing CSF-1 and continued

114 ts in decreased growth of H. influenzae in a chemically defined medium containing Neu5Ac, supporting

115 and the mesendoderm reporter MIXL1-GFP in a chemically defined medium containing the canonical WNT s

116 often observed when microbes are grown in a chemically defined medium containing two sugars (for exa

117 ious data showed that H. pylori grows in the chemically defined medium F-12, but not in other tissue

118 We also show that reprogramming using chemically defined medium favors formation of fully repr

119 . pylori strains were cultured in Ham's F-12 chemically defined medium in the presence or absence of

120 opper, iron, and zinc to the DMSO-containing chemically defined medium induced DNA synthesis and cell

121 grown in continuous culture with a modified chemically defined medium is regulated by growth rate.

122 Halobacterium synthesized cobalamin in a chemically defined medium lacking corrinoid precursors.

123 roduction was significantly decreased when a chemically defined medium lacking N-acetylneuraminic aci

124 give rise to cell lines that proliferate in chemically defined medium lacking serum.

125 nstrated that hemoglobin supplemented into a chemically defined medium significantly and specifically

126 n adipogenesis induction medium, but also in chemically defined medium specifically for osteogenesis,

127 gue profiling after bacterial cultivation in chemically defined medium supplemented with [U-(13)C(6)]

128 We previously described the use of a chemically defined medium supplemented with epidermal gr

129 genes in an S. mutans PTS mutant grown in a chemically defined medium supplemented with glucose.

130 Pasteurella multocida was grown in iron-free chemically defined medium supplemented with hemoglobin,

131 nhancer/promoter, we found that a serum-free chemically defined medium supports formation of embryoid

132 Because RPMI is a more stable and chemically defined medium than M3, the determination at

133 biological role for these proteins, a simple chemically defined medium that supports growth of adult

134 A chemically defined medium that supports the growth of bo

135 well in complex medium, but did not grow in chemically defined medium unless supplemented with the m

136 ains were assessed by monitoring growth in a chemically defined medium where the only source of the e

137 oderm and surface ectoderm, in a serum-free, chemically defined medium with 10-50 ng/ml of FGF-2 indu

138 tential by culturing them as aggregates in a chemically defined medium with TGFbeta1.

139 rs with a predominantly dorsal identity in a chemically defined medium without known morphogens.

140 cells are continuously grown in a completely chemically defined medium without serum supplementation,

141 term dimethylsulfoxide (DMSO) culture, fed a chemically defined medium, are highly differentiated and

142 In a chemically defined medium, exogenous CSP does not induce

143 ells were co-cultured in vitro in serum-free chemically defined medium, functional anchoring junction

144 In a chemically defined medium, growth of the wild-type strai

145 The fucose phenotype, shown in chemically defined medium, is strain specific and linked

146 After growth in a chemically defined medium, stage I (lyophilized) culture

147 When control hESCs were grown in chemically defined medium, their expression of markers o

148 of H2O2 and failed to grow aerobically in a chemically defined medium, unless supplemented with acet

149 Using iron-deficient, chemically defined medium, we determined that H. pylori

150 Using a chemically defined medium, we found that (p)ppGpp accumu

151 , and TGFbeta pathway activity in a minimal, chemically defined medium, we show parallel, robust, and

152 nts and cultured as monolayers in serum-free chemically defined medium.

153 either a Casitone-based complex medium or a chemically defined medium.

154 re BCKAD enzyme activity than cells from the chemically defined medium.

155 or even survive--when plated in mitogen-free chemically defined medium.

156 beta1, TGFbeta2 or TGFbeta3 was added to the chemically defined medium.

157 y role to BrnQ1 during S. aureus growth in a chemically defined medium.

158 ophic for Arg, Gln/Glu, His, Leu, and Trp in chemically defined medium.

159 The observed effects were more striking in a chemically defined medium.

160 ne stifle joints were cultured in serum-free chemically defined medium.

161 nd expansion of the initial SC population in chemically defined medium; (3) a magnetic-activated cell

162 on a rat-tail collagen coated dish and fed a chemically-defined medium supplemented with 2% dimethyls

163 T-II binds to the ESCRT-III subunit Vps20 on chemically defined membranes in a curvature-dependent ma

164 Here, we describe a chemically defined method for generating epicardium and

165 Growth of H. capsulatum mycelia in chemically defined minimal medium produced pigmented con

166 Growth of H. capsulatum yeast in chemically defined minimal medium with L-3,4-dihydroxyph

167 This is the first chemically defined model of the physiological interactio

168 for reduction in cobalt binding by albumin, chemically defined model systems consisting of bovine se

169 tems, we describe a process for synthesizing chemically defined molecular constructs that likewise ac

170 ovide novel, to our knowledge, evidence of a chemically defined, multicomponent lipid system that cou

171 ction by synthetic means, the development of chemically defined multivalent ligands of effectors is s

172 We describe here a chemically defined natural N-linked glycan scaffold that

173 The retrotrapezoid nucleus (RTN) contains chemically defined neurons (ccRTN neurons) that provide

174 In addition, utilizing a chemically defined nonasaccharide acceptor, azidoethyl 6

175 Reconstitution of our uH2B into chemically defined nucleosomes, followed by biochemical

176 eral strategy for engineering the display of chemically defined oligosaccharides on cell surfaces tha

177 gregated and formed osteoclast-like cells in chemically defined osteoclastogenesis medium with 20 ng/

178 This chemically defined platform for cardiac specification of

179 PSC)-derived neuronal cells were cultured on chemically-defined poly(ethylene glycol) (PEG) hydrogels

180 oglia/macrophage precursors were combined on chemically defined polyethylene glycol hydrogels and cul

181 Triptolide (TPT) is a chemically defined, potent immunosuppressive compound is

182 ting inflammation, incubating the cells in a chemically defined protein-free medium that provided a s

183 Here we describe a chemically defined protocol in which hPSCs were initiall

184 Therefore, a chemically defined PRR agonist like poly ICLC can be a r

185 that are low-cost, robust, scalable and use chemically defined raw materials.

186 into complexes for fusion can be studied in chemically defined reactions.

187 Using a chemically defined reconstitution system, we performed a

188 lotting (immunoblotting) in cells grown in a chemically defined rich medium or in a minimal medium.

189 (hES) cells have been developed, but finding chemically defined, robust substrata has proven difficul

190 esired biological activities, and provides a chemically defined, scalable and single cell passaging c

191 Here, we developed a chemically defined, scalable, and reproducible specifica

192 e a mechanism of sensory integration for two chemically defined sensory inputs and may have implicati

193 ion, followed by a 7-day culture period in a chemically defined serum-free basal medium (BM), with or

194 man oral mucosal keratinocytes cultured in a chemically defined serum-free culture system, devoid of

195 or 48 hr in 5% FCS-containing medium or in a chemically defined serum-free medium (ITS).

196 ssessed in cultured granule neurons grown in chemically defined, serum-free medium containing either

197 Using a novel, pluripotent stem cell-based, chemically-defined, serum and feeder cell-free culture s

198 an easily scalable 3D PHH spheroid system in chemically-defined, serum-free conditions.

199 Based on the fusion properties of chemically defined SNARE- proteoliposomes, it has been p

200 A series of novel chemically defined soluble oligomers of the human MHC cl

201 luable in studies aimed at the evaluation of chemically defined structures for toxicity versus immuno

202 splantation of the sensory epithelium onto a chemically defined substrate.

203 Chemically defined substrates for PglC, PglJ, and PglB a

204 s in purifying WaaL homologues and obtaining chemically defined substrates.

205 bunit assembly and coated vesicle budding on chemically defined synthetic liposomes.

206 Therefore, chemically-defined synthetic hydrogels offer benefits ov

207 induced pluripotent stem cells (iPSCs) in a chemically defined system.

208 oglia from human pluripotent stem cells in a chemically defined system.

209 delta) both on unmodified templates and past chemically defined template base lesions (translesion sy

210 NA polymerase delta (pol delta) past several chemically defined template lesions including model abas

211 we report the identification of a family of chemically defined thermoresponsive synthetic hydrogels

212 In this paper we show that feeding chemically defined total parenteral nutrition (TPN) to g

213 the degradation kinetics of substrates with chemically defined ubiquitin configurations.

214 GM2-KLH/QS-21 (GMK) is a chemically defined vaccine that is one of the best devel

215 ycolate (3'-PG)] was examined using a novel, chemically defined vector substrate containing a single,

216 ase activity of pure, recombinant GP12 using chemically defined wall teichoic acid analogs.

217 These chemically defined xeno-free substrates generate more th

218 (PAS) that support self-renewal of hESCs in chemically defined, xeno-free medium.

219 of expanding and differentiating hiPSCs on a chemically defined, xeno-free synthetic peptide substrat

220 Here we develop the first chemically defined, xeno-free, feeder-free synthetic sub