ライフサイエンスコーパス: chromogenic substrate

1 y assaying their peroxidase activity using a chromogenic substrate.

2 with the pathogen of interest reacts with a chromogenic substrate.

3 onjugated antibody against digoxigenin and a chromogenic substrate.

4 by commercially optimized assay coupled with chromogenic substrate.

5 n 13 min using enzymatic amplification and a chromogenic substrate.

6 competitive with respect to the tripeptidic chromogenic substrate.

7 amidolytic activities toward a FIXa-specific chromogenic substrate.

8 e on a monoclonal antibody and assaying with chromogenic substrate.

9 thrombin activity that is measurable with a chromogenic substrate.

10 centration range compared with commonly used chromogenic substrates.

11 o five-fold when compared with commonly used chromogenic substrates.

12 t rely on the availability of radioactive or chromogenic substrates.

13 stantially lower levels of activity on other chromogenic substrates.

14 ability to cleave varied trypsin-susceptible chromogenic substrates.

15 Hydrolysis of the chromogenic substrate 4-methyl umbelliferyl-beta-galacto

16 lly been measured by staining cells with the chromogenic substrate 5-bromo-4-chloro-3-indolyl-beta-d-

17 blue-colony phenotype on plates containing a chromogenic substrate, 5-bromo-4-chloro-3-indolylphospha

18 oside phosphorylase coupling system with the chromogenic substrate 7-methylthioguanosine (MesG).

19 ive binding studies with a thrombin-specific chromogenic substrate, a fluorescein-labeled hirudin pep

20 ermediate, meizothrombin, that has increased chromogenic substrate activity but poor clotting activit

21 egraded approximately twice as fast than the chromogenic substrate, Ala-Pro-2naphthylamide, by dipept

22 larginine p-nitroanilide (Ac-Arg-pNA) as the chromogenic substrate all indicate a cooperative binding

23 The cleavage rates of both a small chromogenic substrate and the factor XIII activation pep

24 tivator was able to degrade both a synthetic chromogenic substrate and the natural substrate fibrinog

25 C may switch the enzyme specificity with the chromogenic substrates and inhibitors but not with the n

26 h a slow conformational change by tripeptide chromogenic substrates and its specific physiological su

27 or factor X, although the affinity for small chromogenic substrates and the efficiency of factor X sc

28 e EC activator did not hydrolyze a factor Xa chromogenic substrate, and recombinant tick anticoagulan

29 ed using a fluorescent thrombin inhibitor, a chromogenic substrate, and SDS-PAGE.

30 h overlapping specificities, enzyme-specific chromogenic substrates, and an antibody specific for act

31 Most chromogenic substrates are required in costly amounts, w

32 X was determined to be functional by using a chromogenic substrate assay after immunoprecipitation wi

33 The hPC was functional as determined by a chromogenic substrate assay after immunoprecipitation.

34 ion of time with radiolabeled proteins and a chromogenic substrate at resolutions of 2-8 s.

35 ortCorin exhibited catalytic activity toward chromogenic substrates but failed to cleave pro-ANP, ind

36 activation and the cleavage of a tripeptidic chromogenic substrate by factor XIa.

37 mpetitively inhibits the hydrolysis of small chromogenic substrates by factor Xa but binds in an orie

38 d that Na(+) stimulated the cleavage rate of chromogenic substrates by FXa or GDFXa approximately 8-2

39 nly a small effect on the hydrolysis of nine chromogenic substrates carrying substitutions at P1, P2,

40 found that all FIXa derivatives cleaved the chromogenic substrate CBS 31.39 with near normal catalyt

41 The fluorogenic substrates were compared to chromogenic substrates (CENTA, nitrocefin, and imipenem)

42 agar assay, zymography, and cleavage of the chromogenic substrate, Chromozym PL.

43 strated hyperbolic, mixed-type inhibition of chromogenic substrate cleavage by factor IXa (K(I) = 88

44 d complex increased 3-fold, and the level of chromogenic substrate cleavage by factor IXa increased m

45 by the factor IXa-phospholipid complex, and chromogenic substrate cleavage by factor IXa, only in th

46 DHG did not affect factor VIIIa half-life or chromogenic substrate cleavage by factor IXa-phospholipi

47 ith the active site, as it failed to inhibit chromogenic substrate cleavage by the factor IXa-PL comp

48 n or the ability of alpha-thrombin to cleave chromogenic substrates, clot fibrinogen, or block alpha-

49 d a variation of the two-stage assay using a chromogenic substrate (COAMATIC; 19% +/- 6.9% of FVIII W

50 Plasmin effectively cleaved a chromogenic substrate corresponding to this cleavage sit

51 Kinetic studies using the chromogenic substrate D-H-Pro-Phe-Arg-4-nitroanilide sho

52 Using chromogenic substrate, DrKIn-I dose-dependently inhibite

53 he mutant, instead, cleaved the APC-specific chromogenic substrates efficiently.

54 ody complexes on the blot are reacted with a chromogenic substrate (either 3.3'-diaminobenzidine [DAB

55 REG shows promise as a chromogenic substrate for E. coli detection and should b

56 A new chromogenic substrate for laccases and peroxidases, 1,8-

57 p-nitroanilide), which can also be used as a chromogenic substrate for plasmin.

58 Here we have identified a new and effective chromogenic substrate for Tdp2 and developed a homogeneo

59 lactopyranosides, were found to be effective chromogenic substrates for an endo-alpha-N-acetyl-D-gala

60 ative micromethod that uses conventional and chromogenic substrates for the identification of medical

61 tivity as much as 70-fold in kcat/K(m) for a chromogenic substrate gamma-Glu-Ala-Gly-pNA.

62 comparison, thrombin-catalyzed hydrolysis of chromogenic substrates gives greater KSIEs and more comp

63 ously by continuous measurement of parabolic chromogenic substrate hydrolysis and fluorescence-based

64 These mutations only had modest effects on chromogenic substrate hydrolysis and the kinetics of fac

65 Chromogenic substrate hydrolysis by the mutant proteases

66 courses of Pg activation by SK monitored by chromogenic substrate hydrolysis had initial rates (v(1)

67 s curves of Pg activation by SK monitored by chromogenic substrate hydrolysis were parabolic, with in

68 y different mechanisms of Na+ enhancement of chromogenic substrate hydrolysis.

69 AP sites is estimated by HRP activity toward chromogenic substrate in an ELISA assay.

70 roups were also distinguishable by using the chromogenic substrates in Dade MicroScan test panels.

71 e, was assayed by the digestion of synthetic chromogenic substrates in protein-matched aliquots of ce

72 Three discontinuous chromogenic substrate initial rate assays were developed

73 ibitor of gamma-thrombin hydrolysis of small chromogenic substrate (K(i) = 0.694 +/- 0.063 nM).

74 several polyanions on the hydrolysis of the chromogenic substrate L-pyroglutamyl-L-prolyl-L-arginyl-

75 rostin, a serine protease, which cleaves the chromogenic substrate (N-benzoyl-L-Ile-L-Glu-L-Gly-L-Arg

76 producing inactive OXA-24 as assayed by the chromogenic substrate nitrocefin.

77 emperatures from 0 to 25 degrees C using the chromogenic substrate o-nitrophenyl-beta-galactoside.

78 s and products directly and does not require chromogenic substrates or lengthy chromatography, was su

79 tracts of these cells readily hydrolyzed the chromogenic substrate p-nitrophenyl-alpha-d-glucopyranos

80 enzyme activity was measured using ABTS as a chromogenic substrate (pH 6.0).

81 effects of the cofactor on the hydrolysis of chromogenic substrates probing the interior of the catal

82 ontrary to results in amidolytic assays with chromogenic substrates, prothrombinase is resistant to i

83 Herein, we propose an alternative chromogenic substrate, resorufin beta-d-glucuronide (REG

84 l 3 enzymes have similar activity toward the chromogenic substrate S-2238, that meizothrombin and mei

85 to the active protease plasmin, cleaved the chromogenic substrate S-2251 and the natural substrate f

86 Activation was monitored using a chromogenic substrate (S-2238) to detect both II(a) and

87 in a plasma clotting assay, to hydrolyze the chromogenic substrate S2366, and to undergo inhibition b

88 Kinetic analysis of these variants using chromogenic substrates showed differences in substrate s

89 with respect to their ability to cleave the chromogenic substrate Spectrozyme PCa, react with protei

90 at the A and B toxins of C. difficile cleave chromogenic substrates that have stereochemical characte

91 in; it used an enzyme-labeled antibody and a chromogenic substrate to provide an amplified visible si

92 Hydrolytic activity of the chromogenic substrate tosyl-glycyl-prolyl-argly-4-nitroa

93 cus faecium (mauve colonies) on the basis of chromogenic substrate use.

94 rhK4 cleaved the chromogenic substrates Val-Leu-Arg-pNA (S-2266), Pro-Phe

95 By utilizing indolepropionyl-CoA as a chromogenic substrate, we could measure the enzyme activ

96 lycosylated pseudoform for several synthetic chromogenic substrates were considerably less (33%-50%)

97 The cleavage of a chromogenic substrate with a single Arg residue at P1 wa

98 he activation was monitored by cleavage of a chromogenic substrate with newly formed plasmin, the rea

99 The chimeric mutant cleaved a FIXa-specific chromogenic substrate with normal catalytic efficiency,

100 er venom at similar rate, but it cleaved the chromogenic substrates with a Gly at the P2 positions po

101 T99Y mutant, similar to FXa, hydrolyzed the chromogenic substrates with a Gly at the P2 positions.

102 or assays, and activities against a range of chromogenic substrates with control plasma.

103 hundredth of the quantity needed for common chromogenic substrates, with an estimated bulk cost of <

104 s were seen in reactivity towards a range of chromogenic substrates, with substantial differences in

105 bitor 6 reduced V(MAX) of FXIa hydrolysis of chromogenic substrate without affecting the K(M), sugges

106 ning with a post-azo-coupling method using a chromogenic substrate (Z-arg-arg-MNA) or by a novel assa