ライフサイエンスコーパス: circular DNA

1 y removing all DNA species other than closed circular DNA.

2 t end products; duplex linear DNA or relaxed circular DNA.

3 e capable of opening and unloading PCNA from circular DNA.

4 bis-intercalating antibiotic, on linear and circular DNA.

5 ends in the DNA backbone for both linear and circular DNA.

6 hibited by 1 microg RNA or covalently closed circular DNA.

7 arch for the minimum energy configuration of circular DNA.

8 demonstrates that the NR-element represents circular DNA.

9 isplace Smc2/4 prebound to a labeled, nicked-circular DNA.

10 ation more significantly for linear DNA than circular DNA.

11 ontributions during the synthesis of relaxed circular DNA.

12 nally includes loss of HBV covalently closed circular DNA.

13 otide fragment annealed to a single-stranded circular DNA.

14 rcularized plus-strand DNA generates relaxed circular DNA.

15 esence of E. coli topoisomerase I and closed circular DNA.

16 sence of transcription-induced stresses in a circular DNA.

17 eins, replicative DNA, and covalently closed circular DNA.

18 to 1/8 the contour length of the uncondensed circular DNA.

19 se activity of Sep1) and the single-stranded circular DNA.

20 adily to supercoiled DNA than to the relaxed circular DNA.

21 e gap in an otherwise duplex 7,100-base pair circular DNA.

22 s, are required for the synthesis of relaxed-circular DNA.

23 en a FRA3B segment and a small polydispersed circular DNA.

24 y of these concatenated sequences into large circular DNA.

25 dR dissociation from linear DNA but not from circular DNA.

26 at it plays a crucial role in the binding of circular DNA.

27 genomes remain episomal, either as linear or circular DNA.

28 g, HBeAg, and intrahepatic covalently closed circular DNA.

29 e torsional rigidities of weakly supercoiled circular DNAs.

30 fication method that exponentially amplifies circular DNAs.

31 to double-stranded nicked or single-stranded circular DNAs.

32 r, 1-long-terminal-repeat (1-LTR), and 2-LTR circular DNAs.

33 s-like DNA (469 bp) as a donor substrate and circular DNA (2,867 bp) as a target substrate.

34 ease in the level of the full-length relaxed circular DNA, a 4- to 5-fold decrease in the plus-strand

35 To make relaxed circular DNA, a template switch is necessary for the RNA

36 on of site-specific labels in long linear or circular DNA allows unambiguous identification of variou

37 ion is due to a less rapid covalently closed circular DNA amplification, leading to lower viremias an

38 The analytical platform includes a circular DNA and a structurally tailored hairpin structu

39 Here, we investigate circular DNA and DNA/RNA hybrid GAGA sequence oligonucle

40 l cure are the presence of covalently closed circular DNA and ineffective/exhaustive immune system.

41 ions increases slowly with concentration for circular DNA and more rapidly for linear DNA, but more s

42 al connection between the occurrence of this circular DNA and subtelomeric recombination events in T.

43 s as both low-copy-number, covalently closed circular DNA and tandemly duplicated, chromosomally inte

44 ion (CSR), including extrachromosomal switch circular DNAs and circle transcripts generated by direct

45 using plasmid DNA, relaxed covalently closed circular DNA, and linear duplex DNA as substrates.

46 pressions include: linear, sheared, bent and circular DNA, and models of the nucleosome superhelix, c

47 rements on sufficiently small (< or =247 bp) circular DNAs, and values in the range C = 300-450 fJ fm

48 ds; (iii) loading mthPCNA onto singly nicked circular DNA; and (iv) supporting mthPolB-catalyzed PCNA

49 d onto freely diffusing, single-stranded M13 circular DNA annealed with fluorescently labeled DNA oli

50 lently closed circular (CCC) and nicked open circular DNA are not substrates for the enzyme.

51 We report the use of small circular DNA as a triplex-directing template for the hig

52 , it would be adventitious to amplify closed circular DNA as closed circular molecules.

53 , it would be adventitious to amplify closed circular DNA as closed circular molecules.

54 of the linking number between the strands of circular DNA at different temperatures.

55 e of Mn2+ ions, TaqN can cleave both RNA and circular DNA at structural bifurcations.

56 Whereas circular DNA became associated with nucleosomes that wer

57 educe the fractions of knotted and catenated circular DNA below thermodynamic equilibrium values.

58 used with different DNA sequences, linear or circular DNA, bulk genomic DNA, recombinant or native Dr

59 polbeta-knockout cells repaired AP sites on circular DNA but not efficiently on linear DNA.

60 ets EcoKI HsdR during dsDNA translocation on circular DNA but not on linear DNA.

61 h 53 or 30 bp DNA molecules also resulted in circular DNAs but these were circular dimers and trimers

62 gyrase introduces negative supercoiling into circular DNA by catalyzing the passage of one DNA segmen

63 region also enhances supercoiling of relaxed circular DNA by the didomain and circularization of shor

64 ts indicate that AP sites can be repaired on circular DNA by the PCNA-dependent pathway in addition t

65 oscopy to image the compaction of linear and circular DNA by the yeast mitochondrial protein Abf2p, w

66 which was isolated in linear (DNA(L222)) and circular (DNA(C222)) forms.

67 only linear and supercoiled DNA, not nicked-circular DNA, can completely displace Smc2/4 prebound to

68 Larger circular DNAs cannot be moved into bacterial cells, but

69 e intermediates, including covalently closed circular DNA (cccDNA) and Dane particles, were detected

70 At the same time, covalently closed circular DNA (cccDNA) and viral mRNA levels both decline

71 ve intermediates including covalently closed circular DNA (cccDNA) are present.

72 nt does not affect initial covalently closed circular DNA (cccDNA) conversion but inhibits the synthe

73 bly, capsid uncoating, and covalently closed circular DNA (cccDNA) formation.

74 ally identifies methylated covalently closed circular DNA (cccDNA) in human liver tissue.

75 ies in the accumulation of covalently closed circular DNA (cccDNA) in nuclei of infected cells.

76 of an intranuclear pool of covalently closed circular DNA (cccDNA) in the liver.

77 ubgenomic RNA from the HBV covalently closed circular DNA (cccDNA) minichromosome, both in cultured c

78 nal template, a long-lived covalently closed circular DNA (cccDNA) molecule, is degraded noncytolytic

79 Covalently closed circular DNA (cccDNA) of hepatitis B virus (HBV) is form

80 HBV covalently closed circular DNA (cccDNA) plays an essential role in HBV per

81 the nuclear pool of viral covalently closed circular DNA (cccDNA) transcriptional template of HBV, w

82 both encapsidated DNA and covalently closed circular DNA (cccDNA) were turned over independently of

83 e hepatitis B virus (HBV), covalently closed circular DNA (cccDNA), has been difficult to study in pa

84 stable minichromosome, the covalently closed circular DNA (cccDNA), in the nucleus of infected hepato

85 intranuclear pool of viral covalently closed circular DNA (cccDNA), resulting in a transient elevatio

86 does not directly affect nuclear HBV closed circular DNA (cccDNA), the genomic form that templates v

87 Covalently closed circular DNA (cccDNA), the nuclear form of hepatitis B v

88 ation and formation of new covalently closed circular DNA (cccDNA), the viral transcriptional templat

89 te or inactivate the viral covalently closed circular DNA (cccDNA), which is a stable episomal form o

90 levels of intrahepatic WHV covalently closed circular DNA (cccDNA).

91 atment from the stable HBV covalently-closed-circular DNA (cccDNA).

92 replication, including the covalently closed circular DNA (cccDNA).

93 formation of hepadnavirus covalently closed circular DNA (cccDNA).

94 of episomal viral genomes [covalently closed circular DNA (cccDNA)] in the nuclei of infected cells.

95 ficantly higher amounts of covalently closed circular DNA compared with wild-type HBV replicating cel

96 lerate the rate of conversion of a linear to circular DNA, compared to extracts from resting cells.

97 associated primarily with covalently closed circular DNA, consistent with this structure being the t

98 Closed circular DNA constructs containing a single 8-oxoguanine

99 Closed circular DNA constructs containing a single TG at a defi

100 cell and molecular biology, double-stranded circular DNA constructs, known as plasmids, are extensiv

101 Circular DNA containing a selectable marker replicates a

102 e have exploited the observation that closed-circular DNA containing an inverted repeat can release t

103 this selection can be transplanted to other circular DNA contexts and retain transcriptional activit

104 on of the repeating sequence compared to the circular DNA controls.

105 Detection and quantitation of 2-LTR circular DNA correlated strongly with viral outgrowth pa

106 found that although viral covalently closed circular DNA declined 20- to 100-fold, integrated viral

107 Circular DNA, derived from lymphocytes of juvenile chann

108 nclusion of a vast excess of non-radioactive circular DNA did not disrupt binding to radioactive f394

109 motherapeutic clearance of covalently closed circular DNA did not involve the replacement of the infe

110 ve endonuclease VII converts the supercoiled circular DNA directly into linear product, indicating th

111 attached to an Ab; thus, in the presence of circular DNA, DNA polymerase, and nucleotides, amplifica

112 AG)n and (CTG)n extrusions in relaxed closed circular DNA do in fact support MutSbeta-, replication f

113 ue of Molecular Cell, Gerhold et al. find no circular DNA during mitochondrial DNA (mtDNA) replicatio

114 Examples of extrachromosomal circular DNAs (eccDNAs) are found in many organisms, but

115 Extrachromosomal circular DNAs (eccDNAs) have been reported in most eukar

116 The DNase was able to digest circular DNA (endonuclease), required both Ca(2+) and Mg

117 e detected eccDNAs were also associated with circular DNA enrichment efficiency.

118 x does not assemble beta onto relaxed closed circular DNA even at low ionic strength.

119 out amplification by using covalently closed circular DNA extracted from the liver of an infected woo

120 Both linear and circular DNA fibers with one to four copies of the chlor

121 ligonucleotide annealed to a single-stranded circular DNA following capture of the annealed complex o

122 , taurocholate uptake, HBV covalently closed circular DNA formation, and expression of all HBV marker

123 types resulting from topoisomerase action on circular DNA forming a particular knot type.

124 performed that amplifies a product only from circular DNA forms that could represent transposition in

125 ly of a histone octamer onto a 192-base pair circular DNA fragment from Caenorhabditis elegans and de

126 Transfection with circular DNA from a TTSuV2 deletion mutant did not produ

127 eplication by clearance of covalently closed circular DNA from infected hepatocytes.

128 ication products most effectively, including circular DNA genome intermediates.

129 During infection, the circular DNA genome of HPV persists within the nucleus,

130 r organelle) contains an approximately 35-kb circular DNA genome of unusually high A/T content (>86%)

131 The synthesis of the hepadnavirus relaxed circular DNA genome requires two template switches, prim

132 Circular DNA genomes distantly related to the plant-infe

133 Multiple small circular DNA genomes encoding replicase proteins plus tw

134 ful replication and segregation of the first circular DNA genomes in precellular ancestors of Gram-ne

135 The circular DNA genomes of PyVs are readily detectable usin

136 re lytic bacteriophages with single-stranded circular DNA genomes, showed high substitution rates (>1

137 d reduction in polymerase activity on primed circular DNA, had dramatically reduced processivity, and

138 duces two smaller DNA circles when acting on circular DNA harboring two recombination sites in head-t

139 removal of undesired linear DNA when nicked circular DNA has been enzymatically prepared from superc

140 y target hepatitis B virus covalently closed circular DNA (HBV cccDNA), the episomal form of the viru

141 r; transfer of SRV provirus and unintegrated circular DNA in blood from the nonviremic donor did not

142 nd, which would nevertheless preserve closed circular DNA in either single-stranded (SS) or double-st

143 generate a small amount of covalently closed circular DNA in LMH cells, a chicken hepatoma cell line

144 analyze HIV-1 2-long terminal repeat (2-LTR) circular DNA in PBMC, which indicates new HIV-1 infectio

145 Circular DNA in the gyrB mutants was more relaxed than i

146 In this study we describe the presence of circular DNA in the nucleus of Trypanosoma brucei.

147 introduce positive supercoils into a closed circular DNA in the presence of bacterial or eukaryotic

148 fic PCR assays and (i) finding WHVNY relaxed circular DNA in the serum samples collected from all sup

149 types of knots or catenanes while acting on circular DNA in vitro and in vivo.

150 ates the formation of nucleosomes on relaxed circular DNA in vitro.

151 esence of DNA nicks, and can occur on closed-circular DNAs in the absence of topoisomerases.

152 input ratio) in supercoiling relaxed, closed circular DNA, in inducing ligase-mediated circularisatio

153 ynthesis but a reduction in the formation of circular DNA, indicating a block after reverse transcrip

154 dence that the duplication was mediated by a circular DNA intermediate.

155 The presence of circular DNA intermediates, a hallmark of active class s

156 revertant that replicated primarily through circular DNA intermediates, but which synthesized elevat

157 This technique uses integration of circular DNA into the bacterial genome via a single-cros

158 from certain mutant yeast strains shows that circular DNA introns exist and are produced by reverse t

159 y of RecG but not for RuvAB, whereas relaxed circular DNA is a poor cofactor for RecG but an excellen

160 does not turnover and additional cleavage of circular DNA is not observed by inclusion of RecBCD, a h

161 We found that unknotted circular DNA is not the most probable state beyond small

162 When circular DNA is used as template in pWGA, 10(8)-fold of

163 e resulting apparent unwinding of the closed circular DNA is used to calculate both ligand unwinding

164 g preference of (dbd)Fos-Jun for linear over circular DNAs is independent of Mg2+ concentration.

165 ranscriptional template or covalently closed circular DNA level.

166 Viral antigens and covalently closed circular DNA levels in liver samples were significantly

167 virus entry since initial covalently closed circular DNA levels were not decreased in IFN-treated ce

168 ies further determined that extrachromosomal circular DNA loss correlated to the entrapment of these

169 The high efficiency of pWGA in amplifying circular DNA makes it a potential tool in diagnosis and

170 tens of thousands of short extrachromosomal circular DNAs (microDNA) in mouse tissues as well as mou

171 The genome of Streptococcus sanguinis is a circular DNA molecule consisting of 2,388,435 bp and is

172 The Escherichia coli chromosome is a circular DNA molecule that is approximately 1000 times c

173 entified a self-propagating extrachromosomal circular DNA molecule that results from intrachromosomal

174 the same 37 genes, whose arrangement in the circular DNA molecule varies only in the relative positi

175 sion), and illegitimate recombination of any circular DNA molecule with an origin-flanking palindrome

176 ate produced from a stable covalently closed circular DNA molecule.

177 ncrease in alignment upon linearization of a circular DNA molecule.

178 between directly repeated sites on the same circular DNA molecule.

179 together, photoligation can be used to form circular DNA molecules and to 'photopadlock' circular DN

180 Circular DNA molecules are then further enriched by rand

181 r 200 guide RNAs (gRNAs) that are encoded in circular DNA molecules called minicircles.

182 Site-specific recombination on supercoiled circular DNA molecules can yield a variety of knots and

183 We have isolated covalently closed circular DNA molecules carrying hybrid pilin loci, but p

184 positive torsional stress in small synthetic circular DNA molecules containing cruciforms with immobi

185 t with a model for DNA condensation in which circular DNA molecules fold several times into progressi

186 enaturation of the duplex DNA; separation of circular DNA molecules from linear DNA molecules; and se

187 patients, the prevalence of extrachromosomal circular DNA molecules harboring amplified genes has bee

188 formation of H-NS nucleoprotein complexes on circular DNA molecules having different arrangements of

189 foreign, single-stranded, covalently closed, circular DNA molecules identical in length to the phi X1

190 ically, on the stability of extrachromosomal circular DNA molecules in cancer cells.

191 telomerator is designed to inducibly convert circular DNA molecules into mitotically stable, linear c

192 resistance gene (MDR1) almost exclusively on circular DNA molecules of approximately 750 and 1500 kb

193 The coexistence of circular DNA molecules of different sizes and, therefore

194 ed of thousands of topologically interlocked circular DNA molecules that form the kinetoplast DNA (kD

195 range is representative of extrachromosomal circular DNA molecules that have been shown to harbor am

196 We have shown that circular DNA molecules up to 250 kb can be efficiently a

197 Linear and circular DNA molecules were photochemically immobilized

198 synapsis, which we investigate using nicked-circular DNA molecules.

199 ay be applicable to the replication of other circular DNA molecules.

200 ents to double-sided adapters to form closed-circular DNA molecules.

201 Bacterial chromosomes are most often circular DNA molecules.

202 esin stimulates intermolecular catenation of circular DNA molecules.

203 s-mtDNA did not reveal detectable amounts of circular DNA molecules.

204 mmalian mitochondria contain several 16.5 kb circular DNAs (mtDNA) encoding electron transport chain

205 Interlocked circular DNA nanostructures, e.g., catenanes or rotaxane

206 synthesis but did not allow formation of the circular DNAs normally found in the nucleus.

207 opoisomerase I (top1) can linearize the open circular DNA of duck hepatitis B virus (DHBV).

208 ic recombination to generate non-replicating circular DNA of the clone or a cell cured of the constru

209 he kinetics of accumulation of serum relaxed circular DNA of WHV demonstrated that the virions produc

210 e cure (ie, eradication of covalently closed circular DNA) of CHB, several challenges in basic resear

211 enzymatic substrate abilities of a series of circular DNA oligonucleotides that are entirely composed

212 31-nucleotide fragments from single-stranded circular DNA only in the presence of the S. cerevisiae s

213 no effect on the level of covalently closed circular DNA or HBV transcripts was observed at late tim

214 everse transcription but before formation of circular DNA or proviral DNA.

215 peat sequences to a palindrome at the Ori of circular DNAs or at the termini of circularized linear D

216 iral DNA, but there was almost no detectable circular DNAs or LTR-LTR junction.

217 matids, consists of thousands of interlocked circular DNAs organized into a compact disk structure.

218 ncy of detectable HIV 2-long terminal repeat circular DNA (P=.013) were significantly lower in CD4+ T

219 e a system that will allow the rescue of any circular DNA (plasmid or phage) using an in vitro transp

220 Therefore, the feasibility of using circular DNA plasmids as standards for 16S rRNA gene est

221 ermediates of in vitro replication of closed circular DNA plasmids.

222 cally, probably by eliminating their relaxed circular DNA precursors and perhaps by destabilizing the

223 licative intermediate DNA, covalently closed circular DNA, pregenomic RNA, and the percentage of WHV

224 To make relaxed circular DNA, primer translocation must occur, resulting

225 on is initiated predominantly on linear, not circular, DNA, producing multi-genomic branched chromoso

226 DNA modification, DNA library screening and circular DNA production.

227 DNA modification, DNA library screening and circular DNA production.

228 site-containing plasmid DNA to two catenated circular DNA products.

229 The product is a 3-kb relaxed circular DNA (RC-DNA) in which one strand is linked to t

230 demonstrated that deproteinized (DP) relaxed circular DNA (rcDNA) of hepatitis B virus (HBV) existed

231 d by conversion of capsid-associated relaxed circular DNA (rcDNA) via unknown mechanisms and exists i

232 apicoplast, whose genome consists of a 35-kb circular DNA related to the plastid DNA of plants.

233 translocation lifetime, which is elevated on circular DNA relative to linear DNA, is important to RA.

234 In reactions with nicked circular DNA (RFII), AN and AN/L3 hydrolyzed exonucleoly

235 By utilizing the helicase enzyme, circular DNA samples can be simultaneously screened and

236 olecule containing hundreds of copies of the circular DNA sequence that remain attached to the Ab and

237 o small, independently replicating linear or circular DNA sequences (amplisomes).

238 , in which very small chemically synthesized circular DNAs serve as efficient templates for generatio

239 assays, and topological analyses with closed circular DNA show that the properties of multiprotein AG

240 Solution studies with closed circular DNA show this compound to be a bisintercalating

241 ngly, gel shift assays using both linear and circular DNA showed that PF0610 does bind DNA, at least

242 Although most virions contain relaxed circular DNA, some contain duplex linear DNA.

243 quences with homology to small polydispersed circular DNAs (spcDNAs).

244 icroDNAs, a family of small extrachromosomal circular DNA species, and tRNA-derived fragments, which

245 based on phage-mid DNA vectors to prepare a circular DNA substrate containing a single-stranded bubb

246 Using a 200-nt primed circular DNA substrate, the combined action of human DNA

247 The partial unwinding of a small-circular-DNA substrate is dependent on the presence of b

248 Using one- and two-site circular DNA substrates we show that CglI does not requi

249 racts, we analyzed correction of mispairs in circular DNA substrates with single defined nicks and me

250 bidirectional replication of double-stranded circular DNA substrates.

251 mp, which can be detected in the presence of circular DNA substrates.

252 one end of the viral donor substrate into a circular DNA target or into other donors.

253 concerted insertion of the two ODNs into the circular DNA target, here termed full-site integration.

254 on for the rolling circle amplification of a circular DNA template and simultaneous overlap extension

255 rm a long single stranded DNA or RNA using a circular DNA template and special DNA or RNA polymerases

256 is observed on an SSB-coated single-stranded circular DNA template in the presence of the beta/gamma

257 tion was a minimum of 35 times faster on the circular DNA template relative to the linear template at

258 When combined with a circular DNA template with a 5' unpaired flap, these pro

259 longation by bacteriophage T7 RNAP on small, circular DNA templates approximately 100 bp in size.

260 ing random primers and phi29 DNA polymerase, circular DNA templates can be amplified 10,000-fold in a

261 Transcription of closed circular DNA templates in the presence of DNA gyrase is

262 by manipulating the types and ratios of the circular DNA templates.

263 circular DNA molecules and to 'photopadlock' circular DNA templates.

264 2/4 to plasmid promote a geometric change in circular DNA that can be trapped as knots by type II top

265 tone-DNA interactions were studied on closed circular DNA that was either moderately or positively co

266 By the design of two different circular DNAs that include recognition sites for two dif

267 era pallida exists as a population of small, circular DNAs that, taken individually, are of insuffici

268 Relaxed circular DNA, the major product, is made when the RNA pr

269 and impaired production of covalently closed circular DNA, the template for DHBV gene expression and

270 For both linear DNA and circular DNA, these contributions are much larger than o

271 We utilized a designed 63 nt circular DNA to encode the synthesis of a self-processed

272 4/Mcm6/Mcm7 assemblies can open to load onto circular DNA to initiate unwinding.

273 ersibly binding DNA ligands that uses closed circular DNA, topoisomerase I (Topo I), and two-dimensio

274 g as loading sites for the enzyme as relaxed circular DNA treated with DNA gyrase, resulted in the hi

275 The presence of RPA and/or pol/prim restored circular DNA unwinding activity of compromised mutants p

276 g and introducing negative supercoiling into circular DNA using free energy derived from ATP hydrolys

277 (LDA), for selective amplification of closed circular DNA using sequence-specific primers.

278 (LDA), for selective amplification of closed circular DNA using sequence-specific primers.

279 xtended opposite the single strand form of a circular DNA vector followed by enzymatic ligation and p

280 reak at a specific site in a double-stranded circular DNA vector.

281 , we first constructed exonuclease-resistant circular DNA via simultaneous ligation of oligonucleotid

282 ormed in two different laboratories: a small circular DNA viral genome (ancient caribou feces associa

283 We show that the genetic diversity of small circular DNA viral genomes in various mammals, including

284 A novel circular DNA virus sequence is reported from grapevine.

285 cken anemia virus (CAV) is a single-stranded circular DNA virus that carries 3 genes, the most studie

286 V) is a recently identified single-stranded, circular DNA virus.

287 Polyomaviruses are small circular DNA viruses associated with chronic infections

288 Anelloviruses are a group of single-stranded circular DNA viruses infecting humans and other animal s

289 Human papillomaviruses (HPVs) are small circular DNA viruses that cause warts.

290 function, even though a stable complex with circular DNA was still observed.

291 transcriptional template, covalently closed circular DNA, was formed by circularization of linear DN

292 urface (HBs) antigens, and covalently closed circular DNA, was observed in HUHEP and HIS-HUHEP mice.

293 s failed to unwind a small origin containing circular DNA whereas replication competent mutants did s

294 DNAs resulted in covalently-closed monomeric circular DNAs which, following histone removal, were pos

295 he predominant pathway gives rise to relaxed circular DNA, while the other pathway yields duplex line

296 he predominant pathway gives rise to relaxed circular DNA, while the other pathway yields duplex line

297 or equilibrium configurations of homogeneous circular DNA with and without bending anisotropy.

298 catenanes with one site in each ring than on circular DNA with two sites, which indicates that the ca

299 ear and weakly strained large (> or =340 bp) circular DNAs yield torsional rigidities in the range C

300 Site-specific recombination on supercoiled circular DNA yields a variety of knotted or catenated pr