ライフサイエンスコーパス: circularization

1 in both primer translocation/utilization and circularization.

2 location/utilization and a partial defect in circularization.

3 They were also invariably defective for circularization.

4 to maintain telomeres and prevent chromosome circularization.

5 ive for primer translocation/utilization and circularization.

6 for primer translocation and 5'r and 3'r for circularization.

7 the second template switch, a process called circularization.

8 g rapid loss of telomeric DNA and chromosome circularization.

9 rate of the protein increased slightly upon circularization.

10 telomeres by a unique pathway of chromosome circularization.

11 se of a DNA template allowed their efficient circularization.

12 king current models insufficient to describe circularization.

13 DNA in an unbent configuration that resists circularization.

14 er a systematic effect of exon length on RNA circularization.

15 y components in unusual ways and promote RNA circularization.

16 diates, cleavage to unit-length strands, and circularization.

17 ly a characteristic essential for plasmid re-circularization.

18 ss of the integrase that was responsible for circularization.

19 d telomere maintenance or through chromosome circularization.

20 pairing during both primer translocation and circularization.

21 d therefore require NHEJ during phage genome circularization.

22 ATM are required for efficient scAAV genome circularization.

23 or Omega, with concomitant failure of genome circularization.

24 by contributing to primer translocation and circularization.

25 own to be important, but not sufficient, for circularization.

26 5E and M5 inhibited primer translocation and circularization.

27 rand DNA synthesis: primer translocation and circularization.

28 irus (DHBV) makes a positive contribution to circularization.

29 DNA amplifications that accompany chromosome circularization.

30 site for the second template switch, termed circularization.

31 Circularization achieved with DNA ligase, followed by li

32 fication procedure, RCA-RCA (Restriction and Circularization-Aided Rolling Circle Amplification), whi

33 e tail-to-head organization is the result of circularization and breakage of a GAPDH retrogene prior

34 rger amounts of pure full-length genomes for circularization and infectivity measurements.

35 Additionally, the circularization and possible fate of HSV genomes are reg

36 rclator, the first tool to automate assembly circularization and produce accurate linear representati

37 Sequence analysis confirmed chromosome circularization and revealed the insertion of adventitio

38 ull-length cDNA libraries, followed by their circularization and the sequencing of the junction fragm

39 o detectable DNA binding based on gel shift, circularization and theta(275) reduction assays.

40 lation undergo telomere deletion, chromosome circularization, and amplification of subtelomeric DNA.

41 at play, however, as not all repeats support circularization, and increasing the stability of the hai

42 s, deletion of additional DNA during plasmid circularization, and insertion of chromosomal DNA fragme

43 ic machinery of these molecules, the role of circularization, and the differences in the possible cir

44 This RNA self-circularization approach to RNA sequencing (RC-Seq) allo

45 template switches, primer translocation and circularization, are required during the synthesis of th

46 Circularization assays showed that HMG-1, but not HMG-I/

47 mina Nextera Mate Pair (NMP) protocol uses a circularization-based strategy that leaves behind 38-bp

48 We suggest that Cp mRNA circularization brings the cytosolic Cp 3'-UTR-binding f

49 cted cells, possibly due to low-level genome circularization by a cellular enzyme.

50 and in vivo studies on splicing-mediated RNA circularization by demonstrating the intracellular produ

51 We combined both PEGylation and circularization by exploiting two distinct sortase enzym

52 providing repetitive sequences suitable for circularization by non-recA-dependent pathways following

53 Protein circularization can significantly impact protein enginee

54 When activation occurred after DNA circularization, cohesion persisted.

55 When gene activation occurred before DNA circularization, cohesion was lost.

56 release into the host nucleus, resulting in circularization, concatemer formation, or chromosomal in

57 ain, which promoted PAB1 oligomerization and circularization, correspondingly accelerated CCR4 deaden

58 approximately 400 nucleotides [nt]) promote circularization cotranscriptionally, whereas pre-mRNAs c

59 lementary 10-23 variants were inactivated by circularization, creating deoxyribozymogens.

60 Circularization (crosslinking the N and C termini) of th

61 The circularization-dependent and orientation-specific scAAV

62 We have developed circularization-dependent and orientation-specific self-

63 ecific cellular proteins involved, the scAAV circularization-dependent vector was used as a reporter

64 Circularization did not lead to a significant thermodyna

65 mple ligation of genomic ends and shows that circularization does not occur by annealing of single-st

66 and biochemical studies reveal that backbone circularization does not prevent the adoption of the nat

67 Circularization does not require protein synthesis in th

68 in the binary, followed by orbital decay and circularization due to tidal dissipation in the stars.

69 template switches (primer translocation and circularization) during synthesis of plus-strand DNA to

70 template switches, primer translocation and circularization, during plus-strand DNA synthesis.

71 This circularization enhances the IRES activity of SHMT1 by f

72 Gel electrophoresis and self-ligation circularization experiments revealed that the CG*C-II du

73 Special categories of splicing such as exon circularization, first and last intron processing, alter

74 enomes were examined for five mutants by RNA circularization followed by cDNA synthesis, amplificatio

75 Here we describe circularization for in vitro reporting of cleavage effec

76 cing requires the essential elements of mRNA circularization, i.e., eukaryotic initiation factor 4G,

77 Deletion of taz1 did not suppress chromosome circularization in cells lacking Rad3/Rad26 and Tel1/Rad

78 circular RNA biogenesis that may account for circularization in genes that lack noticeable flanking i

79 rnal PAI segment was capable of excision and circularization in the donor, and is mobilized as a coin

80 ested by the routing of nuclear viral DNA to circularization in the form of 2-long terminal repeat (2

81 ose termini are capable of undergoing facile circularization in vitro.

82 This can lead to circularization, integration, or the formation of extrac

83 Circularization involves transfer of the nascent 3' end

84 Circularization involves transfer of the nascent plus st

85 eviously demonstrated that selective genomic circularization is a robust in-solution approach for cap

86 The circularization is based on use of a vector that contain

87 However, how RNA circularization is connected to alternative splicing rem

88 we propose that the process of excision and circularization is important in the emergence, pathogene

89 These results mean that the process of circularization is influenced by the earlier steps in DN

90 results suggest that productive rAAV genome circularization is mediated primarily by nonhomologous e

91 s suggest that intramolecular recombination (circularization) is far more efficient than intermolecul

92 of a hospital Klebsiella pneumoniae strain, circularization junctions (CJs) were detected for six GI

93 Herein, circularization kinetics and affinity measurements with

94 estigated by a variety of methods, including circularization kinetics, apparent helical repeat determ

95 nd strand transferase and play a role in the circularization, linearization and possibly integration

96 RNA circularization may be a general replication mechanism f

97 escue the destabilized mutant indicates that circularization may be a useful tool in protein engineer

98 ocessing signal is required, suggesting that circularization may occur post-transcriptionally.

99 ng the efficiency of translation, transcript circularization may serve as an essential structural det

100 ization, and the differences in the possible circularization mechanisms.

101 eir abilities to facilitate ligase-catalyzed circularization of a linear 88 bp DNA molecule, and to r

102 eaction has been used for the intramolecular circularization of a single stranded oligonucleotide whi

103 The catalytic efficiency of the circularization of a single-stranded substrate was 5-fol

104 RNAs'), we identified CircPVT1, generated by circularization of an exon of the PVT1 gene, as a circul

105 one from either Tn916 or Tn5386 promoted the circularization of constructs from the three different t

106 laccase2 flanking introns support efficient circularization of diverse exons in Drosophila and human

107 Circularization of each PLP molecule is dependent upon h

108 We find that circularization of exons is widespread and correlates wi

109 The foundation of this approach is the circularization of fragmented viral RNAs, which are then

110 ial artificial chromosome (BAC) was based on circularization of head-to-tail concatemers of VAC DNA.

111 show that double repeats that are formed by circularization of infecting genomes are rapidly convert

112 ovalently closed circular DNA, was formed by circularization of linear DNA by nonhomologous recombina

113 erfect duplex DNA, nor is it able to mediate circularization of linear duplex DNA.

114 Our findings also suggest that spontaneous circularization of linear Streptomyces chromosomes may b

115 rring atheroprotection, thereby showing that circularization of long non-coding RNAs may alter RNA fu

116 whether a lack of DNA-PKcs activity reduces circularization of rAAV genomes in SCID muscle and wheth

117 of relaxed circular DNA by the didomain and circularization of short DNA fragments (in the presence

118 r, these complexes no longer facilitated the circularization of short DNA molecules and had lost the

119 constructs, called selectors, that guide the circularization of specific DNA target regions.

120 Circularization of T-DNA by the FLP/FRT site-specific re

121 plasmids and RNAi screening, we reveal that circularization of the Drosophila laccase2 gene is regul

122 ase 1 specifically and efficiently catalyzes circularization of the genuine PSTVd monomeric linear re

123 These results suggest that circularization of the HSV genome may not occur early in

124 Circularization of the infecting Mu DNA does not require

125 s in formation of internal organs, including circularization of the intestine and bifurcation of the

126 The model postulated two steps (i) circularization of the linear DNA molecule that had been

127 that are joined by random recombination upon circularization of the linear genome at entry into cells

128 Following circularization of the linear viral genome, DNA replicat

129 ES-mediated translation of SHMT1 whereby the circularization of the mRNA typically provided by the eu

130 es which are said to promote translation via circularization of the mRNA, but in no case has this bee

131 Subsequent circularization of the oligonucleotide label facilitated

132 significantly greater amounts of bending and circularization of the one-base overhang undecamer duple

133 d telomere by homologous recombination; (ii) circularization of the plasmid by non-homologous end-to-

134 ve transposition of Tn916 and is followed by circularization of the transposon and its transfer to a

135 of genome configuration revealed that rapid circularization of the viral DNA occurred on entry, thou

136 integration, excision, and extrachromosomal circularization of these elements, and they have similar

137 an increase in the frequency of excision and circularization of Tn916 caused by expression of integra

138 In contrast, deletions, amplifications, and circularizations of a wild-type strain happened at heter

139 Deletions, amplifications, and circularizations of the linear 8.7-Mb chromosome occurre

140 behaviour of this molecule in assays such as circularization or gel electrophoresis can only be under

141 nd in the appropriate state to permit either circularization or integration of the viral DNA in vivo.

142 Flow-cytometric and digestion circularization PCR analyses revealed that delta c-Rel B

143 nation to S gamma1, as measured by digestion-circularization PCR, is dramatically reduced in STAT6-de

144 c DNA recombination as measured by digestion-circularization PCR.

145 ractions of three essential elements of mRNA circularization, poly(A), PABP, and eIF4G.

146 the level of DNA recombination by digestion-circularization polymerase chain reaction (DC-PCR).

147 ng the 3' elements, as measured by digestion circularization-polymerase chain reaction or by the expr

148 s of the ncRNAs were determined using an RNA circularization procedure.

149 erminal redundancy and the efficiency of the circularization process in those variants.

150 ition of the terminal redundancy made to the circularization process.

151 ) are nearly superimposable, indicating that circularization produces no substantial change in the lo

152 r chromosomal double-strand breaks, and cDNA circularization removes the pro-apoptotic signal.

153 In contrast to the chromosomal circularization reported previously in Schizosaccharomyc

154 In contrast, we find circularization results in a dramatic stabilization of a

155 at the processes of primer translocation and circularization share a common underlying mechanism.

156 at the processes of primer translocation and circularization share a mechanism during which 5E, M, an

157 Basic polymer theory predicts that circularization should lead to a net thermodynamic stabi

158 The circularization site is processed by the tRNA splicing e

159 Here we describe a method, SNP linkage by circularization (SLiC), to identify linkage between CAG

160 ation), which utilizes an efficient DNA self-circularization step during library preparation.

161 We employ an intramolecular circularization step that increases the efficiency of li

162 roidae, the host enzyme catalyzing the final circularization step, has remained elusive.

163 Upon circularization, the torsion constant increased by a fac

164 ear PLP with a target DNA sequence; (ii) PLP circularization through enzymatic ligation; and (iii) qR

165 lizes restriction digestion and whole genome circularization to generate genomic sequences amenable t

166 Although primer translocation and circularization use different donor and acceptor sequenc

167 s end-joining is not required for chromosome circularization was further supported by analysis of sur

168 For these variants, the subsequent circularization was inhibited.

169 its template was restored to 54 nucleotides, circularization was substantially restored.

170 tain specific cases, such as the kinetics of circularization, where the rate-limiting step is a high-

171 es from some Tn916-like elements can promote circularization with termini derived from heterologous t