ライフサイエンスコーパス: circularize

1 ly minimal modification of the protein to be circularized.

2 w nonproductive pathways in which it becomes circularized.

3 king at the oriT before NBU1 has excised and circularized.

4 each of the six K. lactis chromosomes became circularized.

5 nd Oxford Nanopore data, Circlator correctly circularized 26 of 27 circularizable sequences, comprisi

6 e required for an end-ligation reaction that circularizes a portion of the unintegrated viral cDNA in

7 We report that circularizing a DNA template strand encoding a pre-micro

8 le elements were not found to be efficiently circularized after transposase-mediated excision, DNA-PK

9 Here it is shown that most survivors have circularized all of their chromosomes, whereas a smaller

10 false amplicons, primer-dimers etc. are not circularized and degraded by exonuclease treatment.

11 as integrated proviruses but are packaged as circularized and segmented double-stranded DNAs into the

12 a modified deoxyribozyme product that can be circularized and subjected to massive signal amplificati

13 s) rAAV genomes become double stranded (ds), circularized, and/or concatemerized concomitant with a s

14 Lassos, which are capable of binding to and circularizing around complementary target RNAs.

15 small enough periastron distance that it may circularize at an orbital period as short as a few days

16 The double-stranded DNA genome is covalently circularized at both ends.

17 V) persists as episomes in infected cells by circularizing at the terminal repeats (TRs).

18 and that segments within amplified loci are circularized before packaging into nucleocapsids.

19 We designed a retro-isomer and seven circularized "beta-tile" peptide analogs of a typical ra

20 Correctly excised ND5 introns are not circularized but are precisely cleaved near their 3' end

21 which encode a putative homing endonuclease, circularize, but in a way that retains the entire genome

22 and tail-to-tail forms; the concatemers were circularized by homologous or Cre-loxP-mediated recombin

23 on between DNA long-terminal repeats (LTRs), circularized by ligation of the DNA ends or degraded.

24 Each PCR product is then specifically circularized by ligation on a Collector probe capable of

25 oduced into one of the fusion mutant strains circularized by means of end-to-end fusions of the mutan

26 llow non-productive pathways, in which it is circularized by recombination between DNA long-terminal

27 It is excised and circularized by staphylococcal phages phi13 and 80alpha

28 atomic force microscopy that the complex can circularize capped, polyadenylated RNA.

29 Using sonication-based Circularized Chromosome Conformation Capture (4C) couple

30 Using circularized chromosome conformation capture (4C) couple

31 Circularized Chromosome Conformation Capture followed by

32 We combined chromosome conformation capture, circularized chromosome conformation capture, and relate

33 The 165-kb circularized chromosome of Epstein-Barr virus (EBV) is r

34 ad1/Rad9/Hus1/Rad17, and Ku70 groups did not circularize chromosomes.

35 majority of fission yeast cells survives by circularizing chromosomes.

36 scription by RNA polymerase III of purified, circularized cloned DNAs encoding genes for 5S rRNA was

37 In this strategy, DNA templates are circularized, copied multiple times in tandem with a rol

38 er to form the tertiary RNA structure of the circularized DENV genome.

39 The excised and circularized DNA fragments (plasmids) could be propagate

40 ovirus promoter in the capsid containing Cre-circularized DNA is enhanced over linear DNA, as shown i

41 yltransferase dissociates more slowly from a circularized DNA molecule than from the identical linear

42 We found that deletion of net in circularized DNA monomers led to the production of abnor

43 fication technique that can use specifically circularized DNA probes for detection of target nucleic

44 d perturbations are markedly greater for the circularized DNA target.

45 veal that chemical ligation of short ODNs on circularized DNA templates through triplex formation is

46 while concatemer processing gave rise to the circularized DNAs that are packaged into nucleocapsids.

47 facilitated by inverted repeats flanking the circularized exon(s).

48 ly infected cells, KSHV DNA is maintained as circularized, extrachromosomal episomes.

49 s with chronic HBV infection and transfected circularized genome pools or dimeric constructs of indiv

50 (-/-) cells remain linear, and the number of circularized genomes is not affected by the mutation, co

51 TA2) were then isolated using inverse PCR on circularized genomic DNA fragments.

52 sufficient to allow the intervening exons to circularize in cells.

53 that long introns generally flank exons that circularize in Drosophila, but the underlying mechanisms

54 e extrachromosomal complex DNA molecules can circularize in planta.

55 les are detected by padlock probes which are circularized in a strictly target dependent ligation rea

56 The transmitted probe circularized in the cell and failed to integrate into th

57 he spliceosome selects only certain exons to circularize is largely unknown.

58 Thus, the number of PLP circularized is determined by the abundance of target in

59 ng the ends of the viral RNA and effectively circularizing it.

60 g that TCV utilizes an alternative means for circularizing its genome.

61 he Ori of circular DNAs or at the termini of circularized linear DNAs.

62 by these transduction intermediates included circularized monomer and dimer virus genomes in a head-t

63 trum of end-joined molecules ranging from re-circularized monomer to dimer and higher molecular weigh

64 and poly(A) tail-binding protein (PABP) that circularizes mRNAs, promoting translation and stability.

65 We engineered covalently circularized nanodiscs (cNDs) which, compared with stand

66 (RCA) driven by DNA polymerase can replicate circularized oligonucleotide probes with either linear o

67 Circularized oligonucleotides are synthetic, free of the

68 of the PNA-DNA construct a padlock probe is circularized on the free strand.

69 Sequence analysis of the circularized plasmids allowed measurement of relative ac

70 tion; and (iii) qRT-PCR amplification of the circularized PLP after removal of non-circularized templ

71 Elongation of circularized plus-strand DNA generates relaxed circular

72 evidence indicates that eukaryotic mRNAs are circularized, potentially allowing terminating ribosomes

73 for the ligation of a padlock probe, and the circularized probe could in turn be used as a template f

74 spheres (protofibrils), and rings resembling circularized protofibrils (height: ca. 4 nm) were distin

75 We further show that circularized pSLA2 DNA molecules are opened in vivo prec

76 nt shuttle vector, we have demonstrated that circularized rAAV intermediates impart episomal persiste

77 s contain a default pathway that efficiently circularizes ribozyme processed RNAs.

78 A circularized RNA molecule corresponding to the 844 bp re

79 larize, we have constructed a series of self-circularizing RNAs of precisely known size.

80 nomes is now possible, but the final step of circularizing sequences remains unsolved.

81 equilibrated forms of linear and covalently circularized ssRNA molecules.

82 observed ability of hairpin ribozymes with a circularized substrate-binding strand to cleave a circul

83 Subsequently, the circularized target sequences are amplified in multiplex

84 ot carry out rolling circle transcription on circularized templates.

85 of the circularized PLP after removal of non-circularized templates.

86 ws planet-star tidal interactions to rapidly circularize that orbit, decoupling the planets and formi

87 tely, TraI reverses the cleavage reaction to circularize the plasmid strand.

88 te, is required for the template switch that circularizes the genome.

89 trand synthesis and the template switch that circularizes the genome.

90 efect, a less efficient template switch that circularizes the genome.

91 nds to viral mRNA 3' consensus sequences and circularizes the mRNA via interactions with eIF4G.

92 This switch in templates circularizes the nascent genome and is required for the

93 nsposon from the selected vectors; and (iii) circularizing the ensemble of ORFs containing integrated

94 e with the TNF promoter and with each other, circularizing the TNF gene and bringing NFAT-containing

95 es can be obtained, they often spontaneously circularize their chromosomes.

96 w this plasmid, and derivatives of it, to re-circularize through a process similar to gap repair.

97 out tetracycline revealed "joint" regions of circularized Tn5386 composed of 6-bp sequences linking d

98 omplementary '-neo-lox' cassette was used to circularize two linear YACs containing genomic DNA from

99 e of the precursors could not be efficiently circularized under standard conditions.

100 into DF-1 cells, we were able to rescue the circularized unintegrated viral DNA from RSVP simply by

101 ells, we observed that a fraction of genomes circularized upon infection.

102 f conformation-switching aptamer that can be circularized upon interaction with its protein target, t

103 ids with padlock probe (PLP) and then PLP is circularized upon the action of T4 ligase enzyme.

104 YACs up to 670 kb can be efficiently circularized using this vector.

105 ame cells infected with comparable levels of circularizing vector.

106 mately 10.7-kb positive-sense RNA virus that circularizes via RNA-RNA interactions between sequences

107 s when assayed for incomplete, complete, and circularized viral DNA molecules by quantitative PCR amp

108 In addition, circularized viral DNA, a marker of recent nuclear impor

109 mited number of genes are expressed, and the circularized viral genome is maintained as a multicopy e

110 While circularized viral genomes are observed during latency,

111 te cleavage products are able to efficiently circularize, we have constructed a series of self-circul

112 Non-telomeric constructs circularize with first order kinetics and a half-life of

113 d intron sequence (other group I introns are circularized with loss of a short segment of the intron

114 m a primer in the known portion of the gene, circularized with T4 ligase, and then reamplified with P

115 hen DNA fragments in the range of 100 bp are circularized without torsional stress.

116 The circularized YACs were then targeted to the lox sites of