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1 while allowing translation of the downstream cistron.
2 ing translation of the associated downstream cistron.
3 extended to fusion junctions within each alc cistron.
4 activation of translation of the resistance cistron.
5 6 to transactivate expression of an internal cistron.
6 that represses translation of the downstream cistron.
7 ysis of the bacteriophage Q beta readthrough cistron.
8 447 nucleotides from within the readthrough cistron.
9 es the access of ribosomes to the downstream cistron.
10 llow efficient translation of the downstream cistron.
11 ing frame located upstream of the resistance cistron.
12 cistron; and (c) a group I intron in the 43B cistron.
13 s ruled out possible effects of the reporter cistron.
14 omplex during translation of the ermC leader cistron.
15 Gtx or FGF2 5' leaders linked to a reporter cistron.
16 that contains the mir-17-92 microRNA (miRNA) cistron.
17 the ability to support translation of the 3' cistron.
18 t encoded in a different frame within the P3 cistron.
19 16 (VP16) transcription factor as the second cistron.
20 me in which NS2-3 was restored in the second cistron.
21 dependent of the translation of the upstream cistron.
22 if positioned 134 nt upstream of the distal cistron.
23 ach His-tagged and in separate transcription cistrons.
24 usability in the forward design of synthetic cistrons.
25 inserted between the upstream and downstream cistrons.
26 er and that are uncapped or contain multiple cistrons.
27 for polymorphism in the P1, P3, NIa, and CP cistrons.
28 nimal sequence difference was detected among cistrons.
30 TS) that splits the polymerase gene into two cistrons, 43A and 43B, corresponding to N-terminal (gp43
31 ort open reading frame, termed the 5' leader cistron (5'LC), which is 102 bp upstream of the beta2AR
33 ronic mRNAs with the HIS3 gene as the second cistron and 18 random nucleotides in the intercistronic
35 ctor, which has Renilla luciferase as the 5' cistron and firefly luciferase as the 3' cistron, has be
36 microsomal P450 enzyme encoded by the first cistron and the auxiliary protein NADPH-P450 reductase b
37 Renilla luciferase or the EGFP) as the first cistron and the yeast Gal4/viral protein 16 (VP16) trans
38 ge genetic engineering projects require more cistrons and consequently more strong and reliable trans
39 HEG) inserted between the IC-UTS and the 43B cistron; and (c) a group I intron in the 43B cistron.
40 hin the intergenic spacer region of the rDNA cistron, approximately 3 kb upstream (5') of the 18S gen
41 bryophyte) chloroplasts, where the first two cistrons are separated by a spacer region to which no si
43 .4 UTR efficiently translated the luciferase cistron as well, demonstrating the presence of an intern
44 e hairpin, inhibits translation of the first cistron but does not inhibit translation of the cistron
45 ORF2) inhibits translation of the downstream cistron by a process that depends on the uORF2 amino aci
46 and translational control strategies for the cistrons contained therein, but with different specifici
47 hus, ribosomes translating the upstream pabB cistron could be capable of reducing TRAP-dependent cont
48 ions that abolished translation of the first cistron did not, however, affect the IRES-mediated trans
51 icistronic transcripts in which the upstream cistron encoded PTB or PTB deletion mutants (including a
53 cap-independent translation of a downstream cistron encoding Firefly luciferase in a dicistronic exp
57 gh CAT activity was expressed from the first cistron from all of the dicistronic constructs introduce
59 rying an insertion-deletion mutation in this cistron germinated normally; thus, the role of GrmA in s
60 5' cistron and firefly luciferase as the 3' cistron, has been found to generate spliced transcripts.
63 that mediates the translation of the second cistron in a dicistronic mRNA in cultured mammalian cell
65 oth M2 and M3 were expressed from a separate cistron in a VSV mutant background that readily establis
66 t the efficient translation of a second URA3 cistron in dicistronic mRNAs in S. cerevisiae, thereby c
67 that nsp4 to nsp10/11 functions as a single cistron in negative-strand RNA synthesis and analyze rec
72 nd polyamines on translation of a downstream cistron in vivo and support the hypothesis that polyamin
74 nscript cleavage with individually processed cistrons in operons and gene expression regulated by nuc
75 Our results demonstrate that the downstream cistron, in the bicistronic gene, is expressed to a much
77 the IRES-mediated translation of the second cistron, indicating that this IRES-mediated translation
78 ediate positive and negative control of nine cistrons involved in nucleoside catabolism and recycling
79 15q11-q13 and is paternally expressed, each cistron is a candidate for a role in the imprinted Prade
81 ing increase in the translation of the first cistron (luciferase or EGFP) is monitored either by meas
82 activation of the Qbeta coliphage maturation cistron, mediated by the presence of Qbeta replicase.
83 is not due to readthrough from the upstream cistron, nor is it due to translation of cryptic monocis
84 osome entry site, expression from the distal cistron of a dicistronic mRNA increased as a function of
86 ast, the efficient translation of the second cistron of bicistronic mRNAs, directed by two distinct I
87 report an initial characterization of the E1 cistron of HPV type 16 (HPV-16), the most common oncogen
88 nalyzing ribosome stalling at the regulatory cistron of the antibiotic resistance gene ermA, we uncov
89 beta(2)AR upstream peptide of the 5'-leader cistron of the beta(2)AR, and this polymorphism in the b
90 nting protein is translated from a different cistron of the same RNA genome; (iii) 3AB is the most li
92 on and point mutations was created in the CP cistron of wild-type and/or green fluorescent protein-ta
93 , the presence of the 5'-UTR between the two cistrons of a bicistronic mRNA stimulated expression of
94 but not all copies of the nuclear ribosomal cistrons of an individual organism, and the changing rat
96 ent-peptide-dependent stalling at the leader cistrons of several inducible antibiotic resistance gene
99 1, HC-Pro, P3, 6K1, CI, 6K2, NIa-VPg, or NIb cistrons permitted efficient superinfection by WSMV expr
100 e, petB(fbcB) was split genetically into two cistrons, petB6 and petBIV, which encoded two polypeptid
105 ection assays, siRNA knockdown of individual cistrons, RT-PCR to detect mRNA encoded by the bicistron
108 the IRES-mediated translation of downstream cistron, suggesting that the IRES activity requires the
109 ic region increased expression of the second cistron, suggesting that the viral sequence can function
110 etic relatives, gene 43 consists of a single cistron that encodes a PolB family (PolB-type) DNA polym
111 uciferase activity generated from the second cistron that was either equivalent or higher than that o
112 (TriMV) encode two independently functioning cistrons that serve as effectors of SIE at the protein b
113 We analyzed the complete nuclear ribosomal cistron, the complete chloroplast genome, a partial mito
115 e spacer regions of the ribosomal DNA (rDNA) cistron, to test the hypothesis of unequal mutation rate
116 ed over 100-fold-higher levels of downstream cistron translation than did the Renilla open reading fr
117 ative IRESs promote expression of downstream cistrons via splicing rather than internal initiation of
119 eLa cells, the translation of the downstream cistron was increased by 50-fold, demonstrating that tra
121 , with c-myc 5' UTR inserted between the two cistrons, was transfected into both HepG2 and HeLa cells
122 of this stop codon, coding for a second mini-cistron, we could not identify another start codon for g
123 hree subunits from the nuclear ribosomal RNA cistron were compared together with regions of three rep
124 ognition sites mapped in the lake trout rDNA cistron were used to digest genomic DNA into fragments o
126 the 5.8S gene of the nuclear ribosomal gene cistrons) were aligned, guided by ITS transcript seconda
127 beta-galactosidase (betaGAL) from the second cistron whereas little or no betaGAL was expressed in th
128 assay eliminated translation of the upstream cistron while allowing translation of the downstream cis
129 expression of NS5A alone from an additional cistron within a replicon construct gave greater rescue
130 major differences in the length of the rDNA cistron within individual lake trout, minimal sequence d
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