ライフサイエンスコーパス: clasp

1 , and a neuromuscular abnormality (hind-limb clasping).

2 n and disengagement of the membrane-proximal clasp.

3 ctrostatic and hydrophobic interfaces in the clasp.

4 amino acids 550-554 acting as a hydrophobic clasp.

5 e-proximal methionine into a gp41-tryptophan clasp.

6 ins significantly loosens the inner membrane clasp.

7 domain-containing proteins and MT stabilizer CLASPs.

8 exhibited reduced body weight and hind limb clasping.

9 ddition to the previously described hindlimb clasping.

10 ely conserved microtubule-associated protein CLASP [3].

11 ce show behavioral abnormalities including a clasping abnormality of their hind limbs and a habituati

12 These results indicate that C. elegans CLASPs act partially redundantly to regulate astral micr

13 ns during axon guidance and antagonizes both CLASP and Abl activity.

14 we report that Msps is a strong modifier of CLASP and Abl in the retina.

15 ments required for entry involve opening the clasp and expelling the termini.

16 the hPol kappa by interactions between the N-clasp and finger domains combined with stabilization of

17 Our data suggest a model in which CLASP and Msps converge in an antagonistic balance in th

18 e yield a refined model for the alphav beta3 clasp and provide plausible explanations for the effects

19 constrained by the close apposition of the N-clasp and the fingers domain, and therefore can accommod

20 ctions biomechanically as an inter-molecular clasp and thereby facilitates supra-molecular assembly.

21 module and the Fc region facilitated antigen clasping and achieved both high specificity and high pot

22 inclusion size, and lowered the frequency of clasping and footslips on balance beam.

23 ncluding survival, body weight, tremor, limb clasping and open field activities.

24 XMAP215, CLASP, and Crescerin use arrayed tubulin-binding tumor o

25 tubule-associated genes, such as MAP70-5 and CLASP, and receptor genes, such as HERK1 and WAK1, were

26 subunits that is known as the outer membrane clasp, and the key interaction group of the betaA domain

27 nomatous polyposis coli, cytoplasmic dynein, CLASPs, and LIS-1, has been shown recently to target to

28 domain array-containing proteins ch-TOG and CLASP are key regulators of cytoplasmic MTs.

29 CLASPs are conserved MT-binding proteins implicated in t

30 reas the interaction sites with Clip-170 and CLASPs are dispensable.

31 CLIP-associated proteins CLASPs are mammalian microtubule (MT) plus-end tracking

32 CLASPs are microtubule-associated proteins that have a c

33 We show that CLASPs are recruited to the trans-Golgi network (TGN) at

34 CLASPs are spatially regulated microtubule plus end trac

35 Cytoplasmic Linker Associated Proteins (CLASPs) are a conserved class of MT-associated proteins

36 Cytoplasmic linker-associated proteins (CLASPs) are MT-associated proteins thought to organize i

37 Cytoplasmic linker-associated proteins (CLASPs) are proposed to function in cell division based

38 ase 3beta (GSK3beta) directly phosphorylates CLASPs at multiple sites in the domain required for MT p

39 veals encirclement of the DNA by a unique "N-clasp" at the N terminus of Pol kappa, which augments th

40 al studies, KI mice did not display the foot-clasping behavior upon lifting by the tail and lacked an

41 vior and gait dynamics), corrects repetitive clasping behavior, as well as normalizes cued fear-condi

42 d motor deficits, as assessed by rotarod and clasping behavioural tests.

43 This signal, found in the CLASPs beta-arrestin and the autosomal recessive hyperch

44 rker of exposure of residues involved in the clasp between alpha(IIb) and beta(3) cytoplasmic tails,

45 Breakage of a C-terminal clasp between the alpha and beta subunits enhances Mn(2+

46 ) His(722), involved in the formation of the clasp between the tails are also required for skelemin b

47 The peptide is clasped between the N-terminal domain and the transmembr

48 SM proteins, shaped like clasps, bind to trans-SNARE complexes to direct their fu

49 Cell identifies the protein LL5beta as a key CLASP binding platform that mediates communication betwe

50 AP-2 beta2 subunit appendage is a privileged CLASP-binding surface that recognizes a cognate, short a

51 CLASP binds stably to the MT lattice, recruits tubulin,

52 cause they appear to mediate facilitation of clasping by AVT.

53 h, although Peg1 resembled higher eukaryotic CLASPs by physically associating with both Mal3 and Tip1

54 r how ARH can act as an endocytic adaptor or CLASP (clathrin-associated sorting protein) to couple LD

55 no longer localizes to chromosomes, whereas CLASP(CLS-2) depletion does not prevent HCP-1/2 targetin

56 1/2, two redundant CENP-F-like proteins, and CLASP(CLS-2) in Caenorhabditis elegans.

57 apart in the 1 cell embryo, indicating that CLASP(CLS-2) is required for biorientation when chromoso

58 HCP-1/2 and CLASP(CLS-2) localize transiently to mitotic C. elegans

59 In embryos depleted of HCP-1/2, CLASP(CLS-2) no longer localizes to chromosomes, whereas

60 hemical association, depletion of HCP-1/2 or CLASP(CLS-2) resulted in virtually identical defects in

61 sh that the key role of HCP-1/2 is to target CLASP(CLS-2) to kinetochores, and they support the recen

62 le antiparallel bundler PRC1/Ase1 to recruit CLASP/Cls1 to stabilize microtubules.

63 show here that the Schizosaccharomyces pombe CLASP, Cls1p, is a homodimer that binds an alphabeta-tub

64 , we show that the Schizosaccharomyces pombe CLASP, cls1p/peg1p, mediates the stabilization of overla

65 tely, we are interested in understanding how CLASP collaborates with functionally linked proteins to

66 assessments, including analysis of gait and clasping, confirm the presence of a neurological defect.

67 The cofolded complexes have similar "zinc clasp" cores that are augmented by distinct structural e

68 growing microtubules reaching the cortex in CLASP depleted embryos, but the polymerization rate of a

69 Increased GTP-tubulin content within MTs in CLASP-depleted cells suggests that CLASPs facilitate GTP

70 In CLASP-depleted cells, EBs localized along the MT lattice

71 In vitro assays revealed that CLASP directly functions to remove EB from MTs.

72 IP-170, but in the leading edge of the cell, CLASPs display lattice-binding activity.

73 of striatal pathology, begins with hind-limb clasping during tail suspension and tail stiffness durin

74 -arm grooming in which the grooming partners clasp each other's raised palms.

75 mice developed forelimb stereotypy, hindlimb clasping, excessive grooming and hypo-activity prior to

76 in MTs in CLASP-depleted cells suggests that CLASPs facilitate GTP hydrolysis to reduce EB lattice bi

77 A CLASP family MT stabilizer and the depolymerizing kinesi

78 s the Saccharomyces cerevisiae member of the CLASP family of microtubule plus-end tracking proteins a

79 The hormone is bound in a hand-clasp fashion to an elongated, curved receptor.

80 cter (LOS) with the anatomical gastric sling-clasp fibres at the oesophago-cardiac junction (OCJ).

81 inase 3beta likely regulates the affinity of CLASPs for microtubule lattices.

82 hey support the recently proposed model that CLASP functions to promote the polymerization of kinetoc

83 Thus, the two TOGL domains of yeast CLASP have different activities and execute distinct mit

84 Structure predictions suggest that CLASPs have at least two TOGL domains.

85 ormational changes observed near the 'Ca(2+) clasp' hint at the mechanism of Ca(2+)-dependent gating.

86 ming in embryos depleted of either the orbit/CLASP homolog, CLS-2, or FZY-1.

87 Caenorhabditis elegans has three CLASP homologs in its genome.

88 nd beta-subunits known as the inner membrane clasp, hydrophobic packing of a few transmembrane residu

89 Absence of the N-clasp in Dpo4 explains the error-prone processing of the

90 Here, we examine the dynamics of the +TIP CLASP in migrating PtK1 epithelial cells.

91 in, confirming the role of the extracellular clasp in restraining integrin activation.

92 esolution imaging of the Orbit/MAST ortholog CLASP in Xenopus growth cones suggests that this family

93 sembling a baseball catcher's mitt with heme clasped in the palm.

94 e in rotarod performance, and alleviation of clasping in R6/2 mice, establishing a proof-of-principle

95 tubule-growth and nucleation agents, Ran and CLASP, in the establishment of the centrosome-independen

96 In vitro, CLASP increases MT rescue frequency, decreases MT catast

97 Together, these findings suggest that CLASPs influence the MT lattice itself to regulate EB an

98 llow us to precisely classify the roles that CLASP-interacting genes play in MT regulation.

99 g normal EB localization, whereas neither EB-CLASP interactions nor EB tail-binding proteins are invo

100 comprehensive survey of the proteins in the CLASP interactome as MT regulators is missing.

101 rallel genetic and proteome-wide screens for CLASP interactors in Drosophila melanogaster.

102 While the influence of some CLASP interactors on MT behavior is known, a comprehensi

103 viously described functions of several known CLASP interactors, its multiparametric resolution reveal

104 previously identified to be in vivo genetic CLASP interactors.

105 We show that while a point mutation in the clasp interface modestly activates alphaIIb beta3, addit

106 ance of chromosomes (SMC) family of ATPases, clasped into topologically closed rings by accessory sub

107 n of this mechanistically distinct subset of CLASPs into clathrin-coated buds.

108 Our results show that the pol kappa N-clasp is a key structural feature that accounts for the

109 MT association of CLASPs is suggested to be regulated by multiple TOG (tum

110 with a membrane-proximal cytoplasmic domain clasp, is thought to maintain integrins in a low affinit

111 l microtubule stabilization sites containing CLASPs, KIF21A, LL5beta and liprins are recruited to foc

112 irectly to microtubules and are required for CLASP localization to kinetochores.

113 race of an ABO fucose residue by a disulfide-clasped loop, which is inactivated by reduction.

114 rticular, evidence suggests that the MT+TIP, CLASP, may play a pivotal role in the coordination of mi

115 er and as a dimer and further defines that a clasp mechanism may control lipid binding and, ultimatel

116 w functional residues that participate in a "clasp" mechanism to modulate apoA-IV lipid affinity.

117 This indicates that CLASP-mediated stabilization of peripheral MTs, which li

118 tubule (MT) plus-end tracking protein (+TIP) CLASP mediates dynamic cellular behaviors and interacts

119 mediated by the COOH-terminal region of the CLASP microtubule-binding domain and is regulated downst

120 mation of microtubules at the Golgi requires CLASPs, microtubule-binding proteins that selectively co

121 Our work suggests that the clasp might be involved in enzyme kinetics, with the N-t

122 microtubule polymerization (EB1, Mast/Orbit [CLASP], Minispindles [Dis1/XMAP215/TOG]) or depolymeriza

123 that cytoplasmic linker-associated proteins (CLASPs) modulate EB localization at MTs.

124 Beside the well known lock-and-key and clasp motifs, other alternative structural interactions

125 LOS' that likely reflects the gastric sling/clasp muscle fibres at the OCJ.

126 The same is true in orbit/clasp mutants, indicating a pivotal role for this microt

127 reproductive behaviors, including courtship clasping of females.

128 tary movements with dystonic-appearing, self-clasping of limbs, as early as 3 weeks after birth.

129 aning are healthy, but they show an abnormal clasping of the hindfeet when suspended by the tail.

130 Thus, the impact of S. pombe CLASP on interphase microtubule behavior is more closely

131 perturbs the electrostatic interface in the clasp only partially activates alpha(IIb)beta(3) and tha

132 oplasmic linker protein)-associated protein (CLASP), originally identified as a MT plus end-binding p

133 s on a region of the protein that produces a clasp over the active site.

134 behavioral abnormalities including hind limb clasping, overt seizures, motor impairment and context-

135 charomyces pombe CLIP170-associated protein (CLASP) Peg1 was identified in a screen for mutants with

136 g the Dlx5/6-Cre transgene, led to a hindpaw-clasping phenotype and a 50% loss of MSNs without affect

137 Adult Emx-BDNF(KO) mice display a hindlimb clasping phenotype similar to that observed in mouse mod

138 improved the locomotor deficits and hindlimb clasping phenotype, both in male and female mice, and fu

139 omerulus size, and a characteristic hindlimb-clasping phenotype.

140 e tremor, and most animals showed a hindlimb clasping phenotype.

141 test, and increased spasticity as shown by a clasping phenotype.

142 However, both lines developed spasticity (a "clasping" phenotype) at a median age of 21 wk and 29 wk,

143 re more active and developed severe hindlimb clasping phenotypes.

144 Palm-to-palm clasping (PPC) is a distinct style of high-arm grooming

145 mice and male Japanese quail), reproductive clasping (pre-copulatory mounting in newts), and paced m

146 This "antigen clasping" produced an expansive interface where trimethy

147 ntrinsically disordered region of vertebrate CLASP proteins contains two SXIP EB1 binding motifs that

148 for microtubule nucleation at the Golgi via CLASP proteins.

149 gic phenotype including hunchback, hind limb clasp, reduced survival and brain and cortical neuron en

150 otypes including ataxia, front and hind limb clasping, reduced brain size, and smaller neurons.

151 in growth rate and exhibited an altered leg clasp reflex, an altered gait, and defective nursing beh

152 gat3(Delta) mutations exhibited a marked leg clasp reflex, indicating that in the absence of wild-typ

153 Mutation of the clasp region of alphav or beta3 results in a constitutiv

154 arge domain relative to the small domain and clasp region within each subunit of the dimeric enzyme.

155 he large domain relative to the small domain/clasp region, reminiscent of what has been observed in t

156 peptides representing the alphaIIb and beta3 clasp regions promote integrin activation as judged by c

157 l. show that the microtubule binding protein CLASP regulates PIN2 auxin transporter trafficking and s

158 sed MT regulatory paradigm beyond ch-TOG and CLASP, representing a distinct regulator of cilia struct

159 While both gamma-TuNA inhibition and lack of CLASPs resulted in drastically decreased GDMT nucleation

160 henotype of incoordination, involuntary limb clasping, seizures, and premature death.

161 eta, cytoplasmic linker-associated proteins (CLASPs), specialized host +TIPs that control MT formatio

162 Decortication ameliorated hindlimb clasping, striatal neuron atrophy, and huntingtin-positi

163 that only TOGL2 of Saccharomyces cerevisiae CLASP Stu1 binds to tubulin and is required for polymeri

164 Antigen clasping substantially expands the paradigm of antibody-

165 ts on the accelerating rotorod and hind limb clasping tests in transgenic HD mice.

166 helices extend into the cytoplasm and form a clasp that differs significantly from a recently publish

167 ing of the alpha and beta TM domains forms a clasp that regulates integrin activation.

168 le the assembly of outer and inner membrane 'clasps' that hold the alphabeta TMD together to limit tr

169 2) is a clathrin-associated sorting protein (CLASP) that contributes to clathrin recruitment, vesicle

170 ent of clathrin-associated sorting proteins (CLASPs) that independently select different integral mem

171 at terminates in two beta-sandwiches tightly clasping the glucosyltransferase domain.

172 s of GK undergo the same closure motion that clasps the transition state analogue, in contrast to the

173 Previous studies found that "clasping" the alphaIIb head domain to the beta3 tail dec

174 Cell, Efimov and colleagues report a role of CLASPs, the MT plus end-binding proteins, in MT formatio

175 t the border of the outer and inner membrane clasps, thereby decoupling the tilt between these segmen

176 stimuli depends on the ability of Galpha to clasp tightly the GTP molecule that enters the binding s

177 three specificity-conferring NetrinG loops, clasped tightly by matching NGL surfaces.

178 A clasp to mimic juxtamembrane association between the int

179 and beta3 function as a novel extracellular clasp to restrain activation.

180 Mutations in the CLASP TOG domains demonstrate that tubulin binding is cr

181 We find that, although CLASPs track microtubule plus ends in the cell body, the

182 In migrating epithelial cells, CLASPs track MT plus ends in the cell body but bind alon

183 dentified GSK3beta motifs determines whether CLASPs track plus ends or associate along MTs.

184 We propose a model in which CLASP transduces GSK3 activity levels to differentially

185 We propose a mechanism for rescue in which CLASP-tubulin dimer complexes bind along the MT lattice

186 ctivation of integrin adhesion receptors via clasping/unclasping of their membrane-proximal helices.

187 nal helix may regulate the equilibrium-based clasping/unclasping process.

188 Kcne3(-/-) mice exhibited abnormal hind-limb clasping upon tail suspension (63% of Kcne3(-/-) mice >/

189 hain of this asparagine forms an active site clasp via two H-bonds with the residue (Ser85) adjacent

190 The MT-binding region of CLASP was sufficient for restoring normal EB localizatio

191 of high-arm grooming featuring palm-to-palm clasping - we found that matrilineal relationships expla

192 eper insight into the functional partners of CLASP, we conducted parallel genetic and proteome-wide s

193 nal neurons, which play an important role in clasping, were identified by retrograde labeling with te

194 A "Ca(2+) clasp" within the channel's intracellular region acts as