ライフサイエンスコーパス: claudin-1

1 on interactions between HCV and CD81 but not claudin 1.

2 f the barrier-forming tight junction protein claudin-1.

3 In contrast to NRC, Can 10 does not express claudin-1.

4 ger E-box binding homeobox-box1 (ZEB-1) with claudin-1.

5 tion of the tight junction proteins ZO-1 and claudin-1.

6 junction (TJ) proteins occludin, JAM-A, and claudin-1.

7 etween mutant BRAF and the loss of Cdx-2 and claudin-1.

8 ediated mechanism, independent of effects on claudin-1.

9 ial cell markers paired box protein PAX2 and claudin-1.

10 function and plasma membrane localisation of Claudin-1.

11 cyclin D1, c-Myc, COX-2, MMP-7, MMP-14, and Claudin-1.

12 localization, including ZO-1, occludin, and claudin-1.

13 en reported in four tight junction proteins (claudin 1, 14, 16, and zona occludens 2), nine gap junct

14 lls possess mRNAs encoding ZO-1, -2, and -3, claudin-1, -2, -4, and -8, occludin, and connexin-32.

15 e microscopy without affecting the levels of claudin-1, -2, or -3, occludin, or ZO-1.

16 ncoding tight junction components [Occludin, Claudin-1, -2, Zona occludens (ZO-1, -2)].

17 r redistribution and increased expression of claudin-1, -3, and -4.

18 the TJ-associated proteins actin, occludin, claudin-1, -3, and -5, and ZO-1 and -2 were analyzed by

19 with vector controls, levels of occludin and claudins 1, 4, and 7 increased.

20 IL, E-cadherin/CDH1, OCLN, VIM, FN1, and the Claudins 1, 4, and 7.

21 K1 cells using small interfering RNA against claudins 1-4 and 7.

22 junction adhesion molecule 1, occludin, and claudin-1/4 with minimal effects on the cytoplasmic plaq

23 ne phosphorylation and its dissociation from claudins 1/5, induces endothelial TJ disruption and its

24 Whereas claudin-1, -6, and -9 function equally well as entry cof

25 ematical modeling shows that the majority of claudin-1 (76 +/- 5%) is stably localized at the tight j

26 ferentiation and effector functions, such as claudin-1, a Cdx-2 target gene encoding a critical tight

27 Claudin-1, a component of tight junctions between liver

28 product has 91% identity to a mouse protein, claudin-1, a tight junction (TJ)-associated protein.

29 ubset tested expressed significant levels of Claudin-1, a tight junction protein needed for HCV infec

30 e selectively required for the expression of claudin-1, a tight junction protein that was recently id

31 We found that claudin-1, a tight junction protein, and small proline-r

32 Expression and cellular distribution of claudin-1, a tight junction protein, is dysregulated in

33 isease caused by mutations of CLDN1 encoding Claudin-1, a tight-junction (TJ) protein.

34 In addition, overexpression of claudin-1 abrogated the TSA-induced inhibition of invasi

35 is speculated that cholestasis is caused by Claudin-1 absence, leading to increased paracellular per

36 Claudin-1 activates Wnt and phosphotidylinositol-3-kinas

37 Claudin-1 and -2 were 2- to 6-fold upregulation on trans

38 tion of the specific tight junction proteins claudin-1 and -3 in adult brain endothelial cells.

39 ression studies, certain claudins, including claudin-1 and -4, are postulated to increase, whereas ot

40 Claudin-1 and -5, which were heterotypically compatible

41 while still retaining the ability to bind to claudin-1 and -5.

42 ht junctional elements, ZO-1, JAM, occludin, claudin-1 and -5.

43 s against tight junction proteins, ZO-1, and claudin-1 and by measuring apical-basolateral movements

44 Claudin-1 and claudin-10b were detected only in subpopul

45 ccludin and enhanced membrane association of claudin-1 and claudin-2 by IL-15 required the presence o

46 t junctions correlated with up-regulation of claudin-1 and claudin-2 gene transcription.

47 Consistent with this, binding of claudin-1 and claudin-2, but not claudin-4, to S408A occ

48 n-7 protein levels overall, accumulations of claudin-1 and claudin-7 in TJ increased.

49 Although EpCAM knockdown decreased claudin-1 and claudin-7 protein levels overall, accumula

50 nts, showed a similar pattern of increase in claudin-1 and HDAC-2 mRNA expression throughout all stag

51 ytology samples revealed decreased levels of claudin-1 and increased levels of claudin-2 mRNAs.

52 y the observation of decreased expression of claudin-1 and nuclear beta-catenin in brain endothelial

53 B member I, and the tight-junction proteins claudin-1 and occludin have been identified as essential

54 our findings provide evidence for a role of claudin-1 and occludin in epidermal regeneration with po

55 Here, we investigated the TJ proteins claudin-1 and occludin in ex vivo skin wound healing mod

56 meability selectively in ileum (up-regulated claudin-1 and occludin) and a significant reduction in B

57 Cldn-1(53-80) associated with both claudin-1 and occludin, suggesting both the direct inter

58 here are small changes in the trafficking of claudin-1 and occludin.

59 entry factors, the tight junction molecules claudin-1 and occludin.

60 s of E-cadherin decreased, whereas levels of claudin-1 and ZEB-1 increased.

61 croscopic analyses confirmed the presence of claudin-1 and ZO-1 in Z310 cells at cell-cell contact si

62 rmed that tight junction proteins (occludin, claudin-1 and zona occludens-1) are internalized through

63 localization of the tight junction proteins claudin-1 and, to a lesser degree, occludin.

64 in, p120 and beta-catenins, occludin, JAM-1, claudins 1 and 4, and ZO-1) in T84 epithelial cells.

65 of ZO-1 from its binding partners, occludin, claudin 1, and myosin 1C, coincident with opening of tjs

66 ter 6 hours of treatment with HGF, occludin, claudin-1, and beta-catenin were redistributed from the

67 in exchange, but increases exchange of ZO-1, claudin-1, and claudin-2, thereby causing the mobile fra

68 unction proteins zona occludens-1, occludin, claudin-1, and claudin-4, as well as that of desmosomal

69 0 (5HT2a, hc-kit, hTapp1, mTapp2, TARP, NG2, claudin-1, and HPV-18 E6).

70 tion of the TJ proteins, ZO-1, occludin, and claudin-1, and is associated with increased paracellular

71 l intercellular TJ proteins (occludin, ZO-1, claudin-1, and JAM) and subjacent AJ (beta-catenin and E

72 creases in expression of zonula occludens 1, claudin-1, and occludin in rectosigmoid mucosa; the effe

73 lls were transfected with a plasmid encoding Claudin-1, and stable Claudin-1-expressing clones were i

74 d Madin-Darby canine kidney cells, occludin, claudin-1, and ZO-1 were absent from cell-cell contacts

75 n-activated protein kinase (MAPK), occludin, claudin-1, and ZO-1 were recruited to the cell membrane,

76 ounts of the TJ proteins ZO-1, occludin, and claudin-1; and marked disorganization of the actin cytos

77 Occludin, ZO-1, and ZO-2, but not claudin-1, are components of corneal epithelial TJs.

78 uently found in perineurial cells expressing claudin-1 around nerve bundles.

79 Claudin-1 associated with claudin-7 in co-transfection e

80 ly suppressed the mRNA and protein levels of claudin-1 but had only minor effects on the expression o

81 ted associations of EpCAM with claudin-7 and claudin-1 but not claudin-2 or claudin-4.

82 cell complex molecules such as annexin-2 and claudin-1, but also focal adhesion components such as DO

83 Because antibodies directed against CD81 and claudin-1, but not antibodies directed against scavenger

84 try factors CD81, scavenger receptor BI, and claudin-1; by interferon; and by reagents that inhibit N

85 A B cell line expressing high levels of Claudin-1, CD81, and scavenger receptor BI remained resi

86 membrane, including claudin-1-claudin-1 and claudin-1-CD81 interactions.

87 bstitutions of these residues did not impair claudin-1 cell surface expression or lateral protein int

88 testinal permeability in vivo and changes in claudin-1, claudin-2, and myosin IXB genes expression, w

89 molecular weight complex containing at least claudin-1, claudin-2, and occludin; the difference in th

90 docytes used as a negative control expressed claudin-1, claudin-2, and protein gene product 9.5, whic

91 This regulation requires occludin, claudin-1, claudin-2, and ZO-1.

92 onstrate that EMT is associated with loss of claudin-1, claudin-2, occludin, and E-cadherin expressio

93 audin-3 were able to heterotypically bind to claudin-1, claudin-3, and claudin-5.

94 n Transwell permeable supports and expressed claudin-1, claudin-4, and claudin-8-key proteins for tig

95 hilic solutes, and the TJ proteins occludin, claudin-1, claudin-4, junctional adhesion molecule, and

96 significantly, whereas no obvious change of claudin-1, claudin-4, zonula occludens protein 1, and zo

97 s in ZO-2 expression, but not with occludin, claudin-1, claudin-5 or ZO-1 expression in ovine cerebra

98 We conclude that claudin-1, claudin-5, ZO-1, and ZO-2 expression exhibit

99 measure the protein expression of occludin, claudin-1, claudin-5, zonula occludens (ZO)-1, and ZO-2,

100 ker Langerin and the tight junction proteins Claudin-1, Claudin-7, and ZO-2.

101 ctions within the plasma membrane, including claudin-1-claudin-1 and claudin-1-CD81 interactions.

102 The tight junction protein claudin 1 (Cldn-1) has been reported to be down-regulate

103 enger receptor class B type I (SR-BI), CD81, claudin 1 (CLDN1), and occludin (OCLN).

104 arrier composed of reactive astrocyte TJs of claudin 1 (CLDN1), CLDN4, and junctional adhesion molecu

105 (SR-BI), and the tight-junction (TJ) protein claudin 1 (CLDN1).

106 evels of MIR29A and B, but reduced levels of Claudin-1 (CLDN1) and nuclear factor-kappaB-repressing f

107 d that E-cadherin is closely associated with claudin-1 (CLDN1) and occludin (OCLN) on the cell membra

108 munoprecipitation (ChIP) analysis identified claudin-1 (CLDN1) as a prominent HIF target gene.

109 immunosorbant assay using a recombinant CD81-claudin-1 (CLDN1) fusion protein to detect and quantify

110 alysis implicated the tight junction protein claudin-1 (CLDN1) in melanoma progression.

111 Claudin-1 (CLDN1), a tight junction (TJ) protein, has re

112 ve expression cloning approach we identified claudin-1 (CLDN1), a tight junction component that is hi

113 include CD81, scavenger receptor B1 (SR-B1), claudin-1 (CLDN1), and occludin (OCLN).

114 ier and increased expression of TJ proteins, claudin-1 (CLDN1), claudin-23 (CLDN23), occludin, and Zo

115 Other cell surface molecules, such as CD81, Claudin-1 (CLDN1), Occludin (OCLN), SR-BI, and low-densi

116 A tight junction (TJ) protein, claudin-1 (CLDN1), was identified recently as a key fact

117 tight junction molecules occludin (OCLN) and claudin-1 (CLDN1).

118 intestinal epithelial tight junction protein claudin-1 (CLDN1).

119 s B type I (SR-BI, also known as SCARB1) and claudin-1 (CLDN1).

120 actors, including the tight junction protein claudin-1 (CLDN1).

121 ass B type I (SCARB-1), occludin (OCLN), and claudin-1 (CLDN1).

122 Live cell imaging demonstrates CD81 and claudin-1 coendocytosis and fusion with Rab5 expressing

123 nhibitory effect of TGF-beta on occludin and claudin-1 content.

124 cence microscopy revealed that claudin-7 and claudin-1 continually trafficked into lysosomes.

125 Expression of claudin-1 correlated with that of ZEB-1 in human colon t

126 signaling is a possible mechanism underlying claudin-1-dependent changes.

127 ors of these pathways to study their role in claudin-1-dependent regulation of ZEB-1.

128 e structural and functional roles of various claudin-1 domains in HCV entry, we applied a mutagenesis

129 Putative functional intracellular claudin-1 domains were not important.

130 Claudin-1 down-regulates E-cadherin expression by up-reg

131 audin-1 and a synthetic peptide based on the claudin-1 ECL-2 offered no protection to Caco-2 cells.

132 vivo protection from CPE was afforded by the claudin-1 ECL-2 peptide.

133 However, for claudin-1 effects on scratch wound healing were more pro

134 80), because peptides corresponding to other claudin-1 EL domains failed to influence TJ function.

135 The effects of several claudin-1 EL mimetic peptides on epithelial barrier stru

136 Thus, the identification of a critical claudin-1 EL motif, Cldn-1(53-80), capable of regulating

137 oclonal antibody specific for the TJ protein claudin-1 eliminates chronic HCV infection without detec

138 tibodies and HCV particles increase CD81 and claudin-1 endocytosis, supporting a model wherein HCV st

139 In Claudin-1 expressing Can 10 clones, Claudin-1 was locali

140 ith a plasmid encoding Claudin-1, and stable Claudin-1-expressing clones were isolated.

141 hibitors restore cell-cell junctions, rescue claudin 1 expression, and abrogate growth and disseminat

142 (P<0.05), while there were no differences in claudin 1 expression.

143 ckdown of Rab3Gap1 prevented plasma membrane Claudin-1 expression and the formation of a barrier comp

144 urther show a functional correlation between claudin-1 expression and TSA-mediated regulation of inva

145 cells showed a positive correlation between claudin-1 expression and tumor growth and metastasis.

146 Inhibition of claudin-1 expression by HDAC-2-specific small interferin

147 Claudin-1 expression did not differ among groups.

148 Furthermore, we demonstrate that changes in claudin-1 expression have significant effects on growth

149 Genetic manipulations of claudin-1 expression in colon cancer cell lines induced

150 t a novel post-transcriptional regulation of claudin-1 expression in colon cancer cells and further s

151 lecular mechanism(s) underlying dysregulated claudin-1 expression in colon cancer remains poorly unde

152 ver, the mechanisms underlying the increased claudin-1 expression in colorectal cancer remains unknow

153 is issue of Oncogene, Suh et al. reported on claudin-1 expression in hepatocellular carcinoma (HCC),

154 rt an inverse relationship between Smad4 and claudin-1 expression in human colorectal carcinoma tumor

155 t and widely used HDAC inhibitors, inhibited claudin-1 expression in multiple colon cancer cell lines

156 Claudin-1 expression increased in the OB and striatum.

157 Defect in claudin-1 expression increases paracellular permeability

158 thermore, this Smad4-dependent inhibition of claudin-1 expression is independent of TGF-beta signalin

159 hibition of PKC signaling in cells with high claudin-1 expression resulted in decreased claudin-1 exp

160 ry human keratinocytes showed that decreased claudin-1 expression resulted in significantly impaired

161 The decrease in Claudin-1 expression resulting from FASN inhibition was

162 HT29 colon cancer cell lines down-regulates claudin-1 expression through transcriptional repression

163 Claudin-1 expression was evaluated by quantitative rever

164 expressed claudin-1, or SW620 cells in which claudin-1 expression was repressed, to determine the eff

165 Claudin-1 expression was silenced by transfection with s

166 Rab25 knockdown also up-regulated claudin-1 expression, increased transepithelial resistan

167 he major mechanism underlying HDAC-dependent claudin-1 expression.

168 h claudin-1 expression resulted in decreased claudin-1 expression.

169 lular permeability in NRC cells silenced for claudin-1 expression.

170 sed to eDHFR) and the C-terminal YV motif of claudin-1 (fused to GFP) in single microscope images at

171 We found that induction of claudin-1 gene expression in mature podocytes caused pro

172 ched controls, we assessed the filaggrin and claudin 1 genotypes, the phenotypes by dermatoscopy, and

173 Silencing of Claudin-1 in Can 10 clones increased paracellular permea

174 e of HDAC-dependent epigenetic regulation of claudin-1 in colon cancer.

175 Here, we confirm the role of claudin-1 in HCV entry.

176 We studied the role of claudin-1 in hepatic paracellular permeability.

177 Here, we report an increased expression of claudin-1 in human primary colon carcinoma and metastasi

178 These data implicate claudin-1 in melanoma progression.Oncogene advance onlin

179 tein kinase C (PKC) can affect expression of claudin-1 in rat choroid plexus cells, and we observed a

180 nd membrane association of claudin-2 but not claudin-1 in T84 cells.

181 n proteins zonula occludens-1, occludin, and claudin-1 in the ileum following CLP.

182 ata establish the function and regulation of claudin-1 in the perineurium as the major sealing compon

183 ue distribution of occludin, ZO-1, ZO-2, and claudin-1 in the rat cornea.

184 of the tight junction proteins occludin and claudin-1 in the small intestine.

185 also report frequent nuclear localization of claudin-1 in these samples.

186 ulation of a potential metastatic modulator, claudin-1, in a TGF-beta-independent manner.

187 hosphorylation of occludin and ZO-1, but not claudin-1, increased significantly.

188 e resolved the ultrastructural change in the claudin-1-induced SD-TJ transition.

189 unolabeling of kidney proteins revealed that claudin-1 induction destabilized the SD protein complex

190 Mechanistically, claudin-1 interacted with both nephrin and podocin throu

191 ays, AT1002 decreased ZO-1-occludin and ZO-1-claudin 1 interactions coincident with PKCalpha-dependen

192 tly reported that the tight junction protein claudin-1 is a critical HCV entry cofactor.

193 data suggest Rab3Gap1 mediated exocytosis of Claudin-1 is an important component of epidermal barrier

194 Expression of the tight junction protein claudin-1 is dysregulated in colon tumors and associates

195 ell as entry cofactors in endothelial cells, claudin-1 is more efficient in hepatoma cells.

196 Given that claudin-1 is widely expressed in epithelia, we further d

197 the expression of a tight junction protein, claudin-1, is increased during colon carcinogenesis and

198 e gene expression of claudins, in particular claudin-1, is markedly upregulated in the podocyte, acco

199 on of other TJ and AJ proteins such as ZO-1, claudin-1, JAM, beta-catenin, and E-cadherin were down-r

200 rearrangement of key TJ proteins: occludin, claudin-1, junctional adhesion molecule-A, and zonula oc

201 Last, we found that claudin-1 knockdown prevented TEER elevation by PKD inhi

202 T pathway was significantly attenuated after claudin-1 knockdown, and protein levels of extracellular

203 Immunofluorescence study revealed that claudin-1 level was markedly reduced and almost disappea

204 Manipulation of claudin-1 levels in colon cancer cells showed a positive

205 Although LC claudin-1 levels were dramatically reduced in the absenc

206 Can 10 cells, this decrease correlating with claudin-1 levels.

207 This was accompanied by a change of Claudin-1 localisation to the cell surface and interacti

208 key HCV receptor/coreceptor molecules CD81, claudin-1, low-density lipoprotein receptor (LDLr), occl

209 we propose that cell-cell contacts formed by claudin-1 may generate specialized membrane domains that

210 , exhibited upregulated expression levels of claudin-1 mRNA and protein in podocytes.

211 Further studies revealed modulation of claudin-1 mRNA stability by its 3'-UTR as the major mech

212 ond to CPE, while transfectants expressing a Claudin-1 mutant with the corresponding ECL-2 residue ch

213 dition, expression of cell adhesion proteins claudin-1, occludin, and e-cadherin are also increased.

214 O-1 at the tight junction but did not affect claudin-1, occludin, or actin exchange in vitro.

215 lter the expression or distribution of ZO-1, claudin-1, or claudin-4.

216 SW480 colon cancer cells that overexpressed claudin-1, or SW620 cells in which claudin-1 expression

217 Ninety-five renal biopsies were stained for claudin-1 (PEC marker), CD44 (activated PECs), and LKIV6

218 During normal mouse epidermal development, Claudin-1 plasma membrane localisation and Rab3Gap1 cell

219 e expression of Smad4 in Smad4-deficient but claudin-1-positive SW480 or HT29 colon cancer cell lines

220 een HCV E2 and its two co-receptors CD81 and claudin-1 probably triggered the activation of AKT.

221 transcription in 30 min, and an increase in claudin-1 protein by 12 h.

222 947 did not prevent the Smad4 suppression of claudin-1 protein expression in either SW480 or HT29 cel

223 genous occludin protein led to a recovery in claudin-1 protein level, relocation of the zonula occlud

224 kdown resulted in decreases in claudin-7 and claudin-1 proteins that were reversed with lysosome inhi

225 e disruption and mislocalization of ZO-1 and claudin-1 proteins.

226 In contrast, ZO-1 and claudin-1 redistribution occurred well after maximal TER

227 Up-regulation of claudin-1 reduces expression of E-cadherin.

228 ZEB-1 mediates claudin-1-regulated changes in cell invasion and anoikis

229 We investigated the mechanisms by which claudin-1 regulates E-cadherin expression and its effect

230 boxyl termini of zona occludens-3 (ZO-3) and claudin 1, respectively.

231 ductions in the expression of E-cadherin and claudin-1, responses that are standard characteristics o

232 ns appeared normal, immunohistochemistry for claudin 1 showed no reduction in protein amounts, and mo

233 pathway, including MMP9 hemopexin domain or claudin-1 siRNA, enables an opioid peptide ([D-Ala2,N-Me

234 complexes of claudin-7, integrin alpha2, and claudin-1 that normally form in epithelial basolateral c

235 proteins (ie, zonula occludens-1, Occludin, Claudin-1) that critically regulate epithelial paracellu

236 In addition to claudin-1, the transcripts for several other isotypes of

237 layer, and a loss of intracellular levels of claudin-1 to -4.

238 Immunofluorescence staining localized claudin-1 to cell membranes in IL-1beta-treated astrocyt

239 ation and recruitment of ZO-1, occludin, and claudin-1 to the TJ during junctional biogenesis.

240 in vivo Here, we report the generation of a claudin-1 transgenic mouse model with doxycycline-induci

241 r2a knockdown cells was sufficient to rescue Claudin-1 transport to the cell surface.

242 Claudin-1 up-regulates the repressor ZEB-1 to reduce exp

243 n protein amounts, and molecular analysis of claudin 1 was unremarkable.

244 The expression of occludin and claudin-1 was downregulated, and the distribution patter

245 Claudin-1 was higher (P<0.05) in fetuses at 60% of gesta

246 In Claudin-1 expressing Can 10 clones, Claudin-1 was localized at the TJ and paracellular perme

247 ll borders of the superficial layer, whereas claudin-1 was localized mainly in the basal and wing cel

248 In Ppp2r2a knockdown cells, Claudin-1 was located to the cytoplasm and its expressio

249 In the NISCH liver, Claudin-1 was not detected in hepatocytes or cholangiocy

250 taining of a tissue microarray revealed that claudin-1 was overexpressed in melanoma, and aberrantly

251 in human melanoma, cells lacking endogenous claudin-1 were treated with 200 nM phorbol myristic acid

252 nteract with the first extracellular loop of Claudin-1, whereas soluble E2 did not.

253 surface and interaction between Occludin and Claudin-1 which are thought to be required for tight jun

254 ion and integrity through down-regulation of claudin-1, which is a key component of tight junctions.

255 nd claudin-7 was required for association of claudin-1 with EpCAM.

256 n-1beta (IL-1beta) causes rapid induction of claudin-1, with an expression pattern reciprocal to loss

257 and localization of tight junction proteins (claudin-1, ZO-1) were visualized by immunofluorescence.

258 ever, other tight junction proteins, such as claudin-1, ZO-1, and occludin, are unchanged.

259 n 1-fusion proteins of beta-actin, occludin, claudin-1, ZO-1, clathrin light chain A1, and caveolin-1