ライフサイエンスコーパス: claudin-4

1 intermediate cell tight junction components (claudin-4).

2 s, such as zonula occludens-1, occludin, and claudin-4.

3 cells expressing CPE receptors claudin-3 and claudin-4.

4 h claudin-3, did not heterotypically bind to claudin-4.

5 ssion or distribution of ZO-1, claudin-1, or claudin-4.

6 itutively expressing wild-type claudin-3 and claudin-4.

7 s enterotoxin (cCPE) is a natural ligand for claudin-4.

8 situ along with a delay in up-regulation of claudin-4.

9 important roles in the regulatory network of Claudin-4.

10 claudin-7 and claudin-1 but not claudin-2 or claudin-4.

11 udin-2 chimera with a substituted ECL-2 from Claudin-4.

12 ng endogenous RNAs to affect the function of Claudin-4.

13 ick ascending limb of the loop of Henle; and claudin-4, -7, and -8 as determinants of collecting duct

14 ich correlated with the abundant presence of claudin-4, a known CPE receptor, in this villus region.

15 The expression of claudin-4, a protein involved in tight junction complexe

16 laudin 2 expression at 6 hours and decreased claudin 4 and 7 expression at 24 hours; and iv) increase

17 reases in intestinal permeability, preserved claudin 4 and occludin localization at TJs, and decrease

18 EC and is associated with internalization of claudin 4 and occludin.

19 e 24 isoforms identified in mice and humans, claudin-4 and -3 serve as the receptor for Clostridium p

20 In LLC-PK1 cells, claudin-4 and -7 are powerful effectors of paracellular

21 he cation selectivity of the tight junction; claudin-4 and -7 function either as paracellular barrier

22 rier genes, including the IL-34 cytokine and claudins 4 and 8, and showed increased detection of key

23 carcinomas such as lipocalin 2, galectin 3, claudin 4, and cathepsin E.

24 essed by the alveolar epithelium, claudin-3, claudin-4, and claudin-18 are the most prominent; each c

25 permeable supports and expressed claudin-1, claudin-4, and claudin-8-key proteins for tight junction

26 nohistochemical analysis of ApoJ, claudin-3, claudin-4, and epithelial cellular adhesion molecule in

27 yses showed that the beta8 integrin subunit, claudin-4, and S100A1 provided the best distinction betw

28 ung expression of the tight junction protein claudin-4 are associated with alveolar fluid clearance o

29 In particular, claudin-3 and claudin-4 are frequently overexpressed in several neopla

30 However, claudin-3 and claudin-4 are the only family members capable of mediati

31 Rvp.1 (claudin-3) and CPE-R (claudin-4) are members of a larger family of transmembra

32 Using acGFP-claudin 4 as a biosensor of TJ remodeling, we observed i

33 have also implicated certain claudins (e.g., Claudin-4) as receptors for Clostridium perfringens ente

34 es some claudin tight junction proteins (eg, claudin-4) as receptors to form Ca2+-permeable pores in

35 s zona occludens-1, occludin, claudin-1, and claudin-4, as well as that of desmosomal junction protei

36 of non-junctional claudin molecules such as claudin-4 at apical membranes.

37 f receptors in this villus region, and (iii) claudin-4 being an important intestinal receptor for CPE

38 tions with previous studies suggest that the claudin-4 binding site is on a large surface loop betwee

39 Decreased claudin-4, caudin-7, and E-cadherin expression in Lpa1(-

40 Rat fibroblast transfectants expressing a Claudin-4 chimera, where the natural ECL-2 was replaced

41 In contrast, claudin-4 chimeras containing either the first EL domain

42 other tight junctional proteins (claudin-3, claudin-4, claudin-5, occludin, and ZO-1) and adherent j

43 transcription factor, as well as E-cadherin, claudin 4 (Cldn4), and the small GTPase Rab25.

44 Claudin-3 (CLDN3) and claudin-4 (CLDN4) are the major structural molecules tha

45 ponse to hypertonicity, the up-regulation of claudin-4 (Cldn4) expression, and not other claudins, wa

46 egrin subunit, bone morphogenetic protein-7, claudin-4, collagen type IX alpha2, cellular retinoic ac

47 Claudin-4 concentrations are known to increase during lu

48 To define the structural constraints for the claudin-4/Cpe30 interaction, a surface plasmon resonance

49 Immunohistochemical validation revealed that claudin 4, CXCR4, S100A4, and mesothelin were expressed

50 increase during lung injury and the loss of claudin-4 decreases alveolar fluid clearance in mice.

51 ugh a mechanism involving its recruitment of claudin-4 during TJ assembly.

52 also observed using either rclaudin-4 or the claudin-4 ECL-2 peptide in both a preincubation assay an

53 reincubation or coincubation of CPE with the claudin-4 ECL-2 peptide significantly and specifically i

54 However, the claudin-4 ECL-2 peptide was unable to neutralize cytotox

55 These results suggest that claudin-4 ECL-2 peptides should be further investigated

56 her a synthetic peptide corresponding to the claudin-4 ECL-2 sequence can similarly inhibit CPE actio

57 Claudin-4 exchange is not affected.

58 clearance and demonstrate that the amount of claudin-4 expressed may provide specific information reg

59 BALB/neuT mice, which spontaneously develop claudin-4-expressing breast cancer lesions.

60 d cCPE.GST for claudin-4 was confirmed using claudin-4-expressing MDA-MB-468 and SQ20b cells, compare

61 Taken together, (111)In-cCPE.GST targets claudin-4 expression in frank tumors and preneoplastic t

62 (siRNA)-mediated knockdown of claudin-3 and claudin-4 expression in ovarian cancer cell lines reduce

63 regulatory network in gastric cancer whereby claudin-4 expression is reduced by specific miRNAs, whic

64 for known targets, including ROCK and ezrin, claudin-4 expression, and barrier permeability.

65 cancer cells, correlating with claudin-3 and claudin-4 expression.

66 genous RNAs (ceRNAs), resulting in increased claudin-4 expression.

67 nsor of TJ remodeling, we observed increased claudin 4 fluorescence recovery after photobleaching (FR

68 g CPE with soluble recombinant Claudin-4, or Claudin-4 fragments containing ECL-2 specifically blocke

69 ular chloride channel, and delocalization of claudin-4 from the tight junction.

70 that in mouse kidney collecting duct cells, claudin-4 functioned as a Cl(-) channel.

71 n tumors were found to express claudin-3 and claudin-4 genes at significantly higher levels when comp

72 and others have recently found claudin-3 and claudin-4 genes to be highly expressed in ovarian cancer

73 tor alpha increased the proportion of mobile claudin 4 in the TJ.

74 Immunodetection of the TJ protein claudin 4 in WT and mMCP5-deficient mice indicated a sig

75 low or undetectable levels of claudin-3 and claudin-4 in all regions tested by Western and immunohis

76 the level of expression of claudin-3 and/or claudin-4 in chemotherapy-naive and chemotherapy-resista

77 llular ionic selectivity, we expressed human claudin-4 in cultured MDCK cells using an inducible prom

78 this study, we discover a network regulating Claudin-4 in gastric cancer.

79 ned to study the expression of claudin-3 and claudin-4 in human prostate tissue as potential targets

80 In particular, a protective role for claudin-4 in preventing lung injury has emerged.

81 nce correlated directly with the kinetics of claudin-4 induction.

82 Together, our data show that claudin-4 interacts with claudin-8 and that their associ

83 dextran transmucosal flux); ii) occludin and claudin 4 internalization at 12 hours (as assessed by im

84 n the collecting duct cells, the assembly of claudin-4 into TJ strands required its interaction with

85 patocytes, claudin 3 is uniformly expressed, claudin 4 is absent, and claudin 5 is only expressed in

86 Claudin-4 is overexpressed in several premalignant precu

87 Because claudin-4 is overexpressed on some human cancers, the to

88 The second extracellular loop (Ecl2) of claudin-4 is responsible for the binding to the C-termin

89 We observe that Claudin-4 is up-regulated in gastric cancer and is assoc

90 In particular, CLDN4 (encoding claudin-4) is overexpressed in ovarian cancer.

91 es, and the TJ proteins occludin, claudin-1, claudin-4, junctional adhesion molecule, and ZO-1.

92 associated with alveolar fluid clearance or claudin-4 levels.

93 These data suggest that claudin-4 may promote alveolar fluid clearance and demon

94 Transfectants expressing a Claudin-4(N149D) mutant lost the ability to bind or resp

95 ng MDA-MB-468 and SQ20b cells, compared with claudin-4-negative HT1080 cells.

96 significantly higher than in (111)In-GST or claudin-4-negative HT1080 tumors (6.72 +/- 0.18 vs. 3.88

97 els of E-cadherin, desmoplakin, plakoglobin, claudin-4, occludin, zonula occludens 1, and tricellulin

98 Whereas the short peptides bound native claudin-4 on transfected CHO cells in pull-down assays,

99 Knockdown of claudin-4 or -7 expression depressed the permeation of C

100 Loss of claudin-4 or -7 expression elevated the permeation of Na

101 , preincubating CPE with soluble recombinant Claudin-4, or Claudin-4 fragments containing ECL-2 speci

102 Mammary fat pads in mice aged 80 d bore claudin-4-positive aplastic lesions and accumulated (111

103 The uptake of (111)In-cCPE.GST in claudin-4-positive MDA-MB-468 xenograft tumors in athymi

104 Overt mammary tumors were claudin-4-positive, and (111)In-cCPE.GST uptake was 3.2

105 Up-regulation of claudin 4 protein rescued these mobility defects and cyt

106 ous studies have revealed that claudin-3 and claudin-4 proteins are highly overexpressed in ovarian c

107 rotoxin with soluble full-length recombinant claudin-4 (rclaudin-4), which is a CPE receptor, but not

108 with the downstream transmembrane domain of claudin-4 reconstituted the basic structural requirement

109 Claudin-4 reinforce proliferation, invasion, and EMT in

110 GST fusions with claudin-4 revealed that Ecl2 with the downstream transme

111 ified by us (CD44, CD24, EpCAM, aquaporin 5, claudin-4, secretin receptor, claudin-7, V-ros sarcoma v

112 uding p53, HER2/neu, IL-6, kallikrein 6, and claudin-4, some of which may be susceptible to molecular

113 Claudin-4 staining demonstrated a positive correlation w

114 Claudin-4 staining was lower in lungs from donors with g

115 relation [r(s)] = 0.71; P < 0.003); however, claudin-4 staining was not strongly associated with hist

116 disruption of the claudin-8 interaction with claudin-4, the paracellular chloride channel, and deloca

117 t Asn(44) to the corresponding amino acid in claudin-4 (Thr) produced a claudin capable of heterotypi

118 claudin 2, a highly restricted expression of claudin 4 to colonic surface cells, and the finding that

119 binding of claudin-1 and claudin-2, but not claudin-4, to S408A occludin tail is increased relative

120 r peptides selected by phage display against claudin-4-transfected CHO cells and a 12-mer Cpe mutant

121 In contrast, expression of claudin-3 and claudin-4 was absent in adjacent normal brain tissue.

122 overexpressed epithelial monolayers and that claudin-4 was also restricted from intercellular contact

123 The affinity of radiolabeled cCPE.GST for claudin-4 was confirmed using claudin-4-expressing MDA-M

124 ely charged lysine residue at position 65 of claudin-4 was critical for its anion selectivity.

125 Claudin-4 was localized to Triton X-100-soluble gradient

126 ferentially isolated as a homodimer, whereas claudin-4 was monomeric.

127 Claudin-4 was observed to interact with claudin-8 using

128 blot analysis, mRNAs for both claudin-3 and claudin-4 were expressed at high levels in prostate tiss

129 immunohistochemical analysis, claudin-3 and claudin-4 were expressed frequently in metastases from b

130 We found that although claudin-3 and claudin-4 were heteromerically compatible when expressed

131 Importantly, Cpe30 retained its binding to claudin-4 when fused to the C terminus of influenza hema

132 a claudin capable of heterotypic binding to claudin-4 while still retaining the ability to bind to c

133 d (residues 194-319) binds to purified human claudin-4 with a 1:1 stoichiometry and affinity in the s

134 peptide also showed significant affinity for claudin-4 with this SPR assay, suggesting that a short p

135 tly, whereas no obvious change of claudin-1, claudin-4, zonula occludens protein 1, and zonula occlud