ライフサイエンスコーパス: claudin-5

1 phosphorylation of occludin, ZO-1, ZO-2, and claudin-5.

2 ing zonula occludens-1 (ZO-1), occludin, and claudin-5.

3 art by tight junction (TJ) proteins, such as claudin-5.

4 as shown by immunostaining for occludin and claudin-5.

5 degradation of the TJ proteins occludin and claudin-5.

6 otypically bind to claudin-1, claudin-3, and claudin-5.

7 n tight junction (TJ) proteins: occludin and claudin-5.

8 ctive diffusion of small molecules and makes claudin-5 a possible target for the development of drugs

9 In this issue, Nitta et al. demonstrate that claudin-5, a transmembrane protein of TJs, is a critical

10 esidues in the first extracellular domain of claudin-5 altered the properties of the tight junctions

11 Claudin 5 and eNOS Western blot analysis were used to me

12 n molecules zona occludens-1, claudin 3, and claudin 5 and other pathways critically involved in tran

13 ntine) rescued cerebrovascular expression of claudin-5 and blood-brain barrier permeability to both e

14 for the tight junction proteins occludin and claudin-5 and examined by confocal microscopy.

15 s in transendothelial electrical resistance, claudin-5 and occludin became internalized via caveolae

16 lation of cytoplasmic domains of recombinant claudin-5 and occludin by RhoK.

17 om a sucrose gradient showed the presence of claudin-5 and occludin in the same fractions that contai

18 However, total expression of claudin-5 and occludin remained unchanged except for a n

19 The tight junction proteins claudin-5 and occludin showed reduced surface expression

20 A downregulation of TJ proteins, claudin-5 and occludin, paralleled monocyte migration in

21 ral endothelial cell tight-junction proteins claudin-5 and occludin.

22 ion of transferrin receptor, p-glycoprotein, claudin-5 and occludin.

23 Claudin-5 and vascular endothelial-cadherin (VE-cadherin

24 ced in vivo permeability and dissociation of claudin-5 and VE-cadherin at junctional complexes.

25 EC expression paralleled decreased levels of claudin-5 and VE-PTP.

26 HIV-1-induced IL-6, diminished HIV-1-induced claudin-5 and ZO-1 down-regulation, and blocked HIV-1- a

27 gulation, exogenous glucocorticoids regulate claudin-5 and ZO-2 in vivo at some, but not all ages, an

28 meability was correlated with an increase in claudin-5 and zonula occludens-1 immunofluorescence at c

29 GCs also reduced levels of occludin, claudin 5, and caveolin 1, proteins central to blood-bra

30 Tight junction components ZO-1, claudin 5, and occludin were decreased at both the trans

31 (elevated levels of tight-junction protein, Claudin 5, and reduced S100B levels in periphery).

32 tial for GC-mediated expression of occludin, claudin-5, and barrier induction, and the p54/PSF hetero

33 ltiple junctional genes, including occludin, claudin-5, and cadherin-9.

34 ht junction protein down-regulation of ZO-1, claudin-5, and JAM-1 in HBMEC.

35 tight junction proteins (zonula occludens 1, claudin-5, and occludin), astrocyte activation, IgG extr

36 unctional phenotype as assessed by occludin, claudin-5, and ZO-1 immunoreactivities.

37 and protein expression of ZO-1, occludin and claudin-5 are prevented with inhibition of nociceptive i

38 akage, and elevated levels of syndecan-1 and claudin-5 are strongly associated with severe plasma lea

39 hosphorylated occludin at T382 and S507, and claudin-5 at T207 from full-length recombinant occludin

40 These new insights into the functions of claudin-5 at the molecular level in tight junctions may

41 chizophrenia in 22q11DS, leading to 75% less claudin-5 being expressed in endothelial cells.

42 e tight junction proteins ZO-1, occludin and claudin-5 between endothelial cells.

43 d the direct phosphorylation of occludin and claudin-5 by RhoK at specific sites, which was increased

44 bution of TJ proteins (occludin, ZO-1, ZO-2, claudin-5) by CCL2.

45 the endothelial cell tight junction protein claudin-5 (Cldn5) and abnormal blood vessel morphology i

46 helial transmembrane tight junction proteins claudin-5 (CLN-5) and occludin (OCLN) as targets of VEGF

47 ential endothelial TJ proteins, occludin and claudin-5, contribute and possibly disrupt BBB integrity

48 al-time RT-PCR assays for BCRP, occludin and claudin-5 demonstrated no significant differences betwee

49 Claudin-5 depletion only mimicked ZO-1 effects on barrie

50 Unexpectedly, knockout of claudin-5 did not result in a general breakdown of TJs b

51 Claudin-5 distribution was altered in small- to medium-s

52 vation of PPAR-gamma prevented HIV-1-induced claudin-5 down-regulation and significantly reduced vire

53 e HIV/AIDS models, we demonstrated decreased claudin-5 expression and increased macrophage infiltrati

54 sing siRNA by itself caused up-regulation of claudin-5 expression and partial protection from cytotox

55 paracellular pathway, demonstrated decreased claudin-5 expression at 24 h, and an increase at 48 and

56 ury and now we demonstrate a contribution of claudin-5 expression in IL-4-induced protection.

57 chotic medications dose-dependently increase claudin-5 expression in vitro and in vivo while aberrant

58 Increased claudin-5 expression resulted in increased transmembrane

59 The increased claudin-5 expression was not limited to the junction.

60 gs, Efavirenz, but not other NNRTIs, altered claudin-5 expression, increased endothelial permeability

61 of the tight junctions formed in response to claudin-5 expression.

62 s necessary for GC induction of occludin and claudin-5 expression.

63 ein claudin-5, with concomitant reduction in claudin-5 expression.

64 ailing CMs displayed downregulated ephrin-B1/claudin-5 gene expression linearly related to the ejecti

65 Here, we show that a variant in the claudin-5 gene is weakly associated with schizophrenia i

66 population who are haploinsufficient for the claudin-5 gene.

67 ation and intracellular fate of occludin and claudin-5, green fluorescent protein fusion proteins of

68 association with the tight junction protein, claudin-5, has previously been identified.

69 eactivity and ConA binding, but no change in claudin-5 immunoreactivity was detected.

70 e expression of cleaved Notch-1, villin, and claudin 5 in colon samples from mice and humans.

71 ced expression of cleaved Notch, villin, and claudin 5 in colonocytes and significantly reduced the p

72 tion of tight junctions, we expressed murine claudin-5 in Madin-Darby canine kidney II cells.

73 suppression, reinforcing the crucial role of claudin-5 in normal neurological function.

74 induces upregulation of the junction protein claudin-5 in porcine ECs through activation of Jak/STAT6

75 while aberrant, discontinuous expression of claudin-5 in the brains of schizophrenic patients post m

76 eno-associated virus-mediated suppression of claudin-5 in the mouse brain results in localized BBB di

77 n of TJ transmembrane proteins (occludin and claudin-5) in increased permeability of the brain endoth

78 s of occludin, E-cadherin, beta-catenin, and claudin-5 increased significantly, whereas no obvious ch

79 s, occludin, junction adhesion molecule, and claudin-5, induced by TNF-alpha in BCECs and consequentl

80 niformly expressed, claudin 4 is absent, and claudin 5 is only expressed in endothelial junctions.

81 Claudin-5 is a protein component of many endothelial tig

82 Claudin-5 is expressed in endothelial cells forming part

83 ve demonstrated that the expression level of claudin-5 is governed by the expression of VE-cadherin.

84 Claudin-5 is necessary and sufficient to diminish alveol

85 silon) phosphorylation, and up-regulation of claudin-5 is suppressed by PKCepsilon inhibitor peptide

86 tissues, we demonstrated down-regulation of claudin-5 (marker of pulmonary barrier integrity), down-

87 t junctional proteins (claudin-3, claudin-4, claudin-5, occludin, and ZO-1) and adherent junctional p

88 mRNAs and protein for claudin-5, occludin, and zona occludens 2 were also redu

89 protein expression of collagen-IV, laminin, claudin-5, occludin, and zonula occludens protein 1 was

90 detected in junctions of the duct epithelia, claudin 5 only in junctions of acinar cells, whereas cla

91 xpression, but not with occludin, claudin-1, claudin-5 or ZO-1 expression in ovine cerebral cortices.

92 can-1 (OR = 1.004; 95% CI = 1.000-1.008) and claudin-5 (OR = 1.038; 95% CI = 1.004-1.074) had an adju

93 trate that in response to alcohol, increased claudin-5 paradoxically accompanies an increase in parac

94 Critically, a claudin-5 peptide mimetic reverses the deleterious effec

95 rotected BBB integrity and reversed occludin/claudin-5 phosphorylation associated with monocyte migra

96 nase (RhoK) activation mediates occludin and claudin-5 phosphorylation resulting in diminished barrie

97 Claudin-5 protein expression was 69% and 73% higher (P<0

98 Therefore, expression of claudin-5 selectively decreased the permeability to ions

99 ble 'knockdown' mouse model, we further link claudin-5 suppression with psychosis through a distinct

100 develop seizures and die after 3-4 weeks of claudin-5 suppression, reinforcing the crucial role of c

101 on occludin (T382 and S507) and one site on claudin-5 (T207).

102 of either cysteine abolishted the ability of claudin-5 to increase transepithelial resistance, and mu

103 To test the contribution of claudin-5 to this barrier function of tight junctions, w

104 ld increase in transepithelial resistance in claudin-5 transductants and a reduction in conductance o

105 monosaccharides was significantly changed in claudin-5 transductants compared to controls.

106 07 from full-length recombinant occludin and claudin-5 transiently expressed in COS-7 cells and mouse

107 roteins ZO-1, JAM-2, Occludin, Claudin-3 and Claudin-5, using in vitro cultures of the primary brain

108 ey roles in vascular stabilization including claudin-5, vascular endothelial-protein tyrosine phospha

109 EC association increases Ezh2 recruitment to claudin-5, VE-PTP, and vWf promoters, causing gene downr

110 ve complex-2) binding to promoter regions of claudin-5, VE-PTP, and vWf.

111 Expression of cleaved Notch-1, villin, or claudin 5 was not detected in RAG1(-/-) colonocytes; the

112 Claudin-5 was higher at 60% than 70% of gestation, and t

113 Claudin-5 was higher in dexamethasone than placebo-treat

114 ession and activation of STAT1 and decreased claudin-5 were observed in microvessels from autopsied b

115 dherin immunocytochemistry and expression of claudin-5, which were all unaltered by high glucose.

116 eins, zonula occluden-1 (ZO-1), occludin and claudin-5 with CIP.

117 the promoter for the tight junction protein claudin-5, with concomitant reduction in claudin-5 expre

118 bsence of tight junction proteins (occludin, claudin-5, ZO-1 and JAM-1) in the parenchymal blood vess

119 We conclude that claudin-1, claudin-5, ZO-1, and ZO-2 expression exhibit differentia

120 L-6 expression, and diminishes expression of claudin-5, ZO-1, and ZO-2 in HBMECs.

121 endothelial cells expressed the TJ proteins claudin-5, ZO-1, and ZO-2; HIV-1 decreased TJ proteins e

122 In wild-type CMs, ephrin-B1 interacted with claudin-5/ZO-1 complex at the lateral membrane, whereas

123 e protein expression of occludin, claudin-1, claudin-5, zonula occludens (ZO)-1, and ZO-2, and a TJ a

124 ion-associated proteins, including occludin, claudin-5, zonula occludens-1, junctional adhesion molec