ライフサイエンスコーパス: click chemistry

1 atalyzed Huisgen 1,3-dipolar cycloaddition ("click chemistry").

2 ions, has been synthesized via alkyne-azide "click chemistry".

3 cyclic alkyne reagents for fast and tunable "click chemistry".

4 quipped with a propargylglycine residue for "click chemistry".

5 ed azide-alkyne cycloaddition, often termed "click chemistry".

6 and an alkyne moiety for biotinylation via "click chemistry".

7 oselective fluorescence tagging by means of 'click chemistry'.

8 alyzed azide-alkyne cycloaddition (Sharpless click chemistry).

9 )-catalyzed [3 + 2] cycloaddition reaction ("click" chemistry).

10 atalyzed Huisgen 1,3-dipolar cycloaddition ("click" chemistry).

11 proteins with a non-canonical amino-acid and click chemistry.

12 hen assembled in a combinatorial fashion via click chemistry.

13 dipolar cycloaddition reaction, a variant of click chemistry.

14 none for photocrosslinking and an alkyne for CLICK chemistry.

15 containing cellular proteins by azide-alkyne click chemistry.

16 owing since its stimulation by the advent of click chemistry.

17 d to azide functionalized VHH domains, using click chemistry.

18 oxynaphthalene (DNP) recognition units using click chemistry.

19 hus going far beyond the original purpose of click chemistry.

20 l PG using d-amino acid dipeptide probes and click chemistry.

21 ementary oligonucleotides, and bioorthogonal click chemistry.

22 ed exploiting 5'-ethynyluridine labeling and click chemistry.

23 like receptors (TLRs) and also determined by click chemistry.

24 synthesized by convergent methodology using click chemistry.

25 s novel clubbed 1,2,3-triazole hybrids using click chemistry.

26 ovalent cross-linking using copper catalyzed click chemistry.

27 vator, 17-alpha-ethynylestradiol (17EE), via click chemistry.

28 ns for the use and applications of thiol-ene click chemistry.

29 re conjugated to IRDye 800 using copper-free click chemistry.

30 labeled with mass tags to the bound probe by Click chemistry.

31 amine and pyrene azide using Cu(I)-catalyzed click chemistry.

32 ional design of new reagents for copper-free click chemistry.

33 high-relaxivity multiplexed probes utilizing click chemistry.

34 ring-expansion metathesis polymerization and click chemistry.

35 tabolically labeled glycans with copper-free click chemistry.

36 h intrinsic reactivities desired for Cu-free click chemistry.

37 immobilization of azides using catalyst-free click chemistry.

38 uran salicylic acid derivatives assembled by click chemistry.

39 upling of an affinity tag using alkyne-azido click chemistry.

40 oinitiators, which makes it truly two-photon click chemistry.

41 lyzed [3 + 2] azide-alkyne cycloaddition, or click chemistry.

42 functionalized leader nucleoside monomers by click chemistry.

43 -selective peptide cyclo(FRGDLAFp(NMe)K) via click chemistry.

44 dibenzocyclooctyne (DBCO)-Cy5 by subsequent click chemistry.

45 cently emerging applications in the field of click chemistry.

46 -selective peptide cyclo(FRGDLAFp(NMe)K) via click chemistry.

47 und to SH functionalities applying thiol-ene click chemistry.

48 king such functionality available for use in click chemistry.

49 ygen-sensing dendrimers through azide-alkyne click chemistry.

50 onversion to the corresponding triazoles via click chemistry.

51 nm in length were grown layer-by-layer using click chemistry.

52 orophores via bioorthogonal copper-catalyzed click-chemistry.

53 incorporation of unnatural amino acids and "click" chemistry.

54 Ru(II) polypyridyl chromophores coupled via "click" chemistry.

55 because 1,2,3-TRZs are easily obtained via "click" chemistry.

56 diated amine-to-nitrile addition, a form of "click" chemistry.

57 hod using DNA methyltransferase (Mtase) and "click" chemistry.

58 receptor (AVP V(2)R) was synthesized using "Click" chemistry.

59 ole and triazole derivatives prepared using "click" chemistry.

60 tion of alkyne-derived motifs for so-called "click" chemistry.

61 ructed and postsynthetically modified using "click" chemistry.

62 d addition of azides and alkynes, a type of "click" chemistry.

63 red to the surface utilizing bio-orthogonal "click" chemistry.

64 yzed azide-alkyne cycloaddition reaction in "click" chemistry.

65 sized using stepwise imine condensation, or "click" chemistry.

66 rivatized conducting PEDOT electrodes using "click" chemistry.

67 with an azide-containing fluorophore using 'click' chemistry.

68 atalyzed 1,3-dipolar Huisgen cycloaddition ("click") chemistry.

69 e being discussed, involving orthogonal and 'click' chemistries.

70 rged precursors, causing them to combine by "click" chemistry 1,000,000 times faster than without acc

71 "Click chemistry" 1,3-dipolar cycloaddition between alkyn

72 he Fe(III)-HOPO ICP particles by copper-free click chemistry afforded colloidally stable nucleic-acid

73 The orthogonality of oNQM-thiol and azide click chemistry allowed for the development of a sequent

74 cular precursors react through a copper-free click chemistry, allowing for the direct encapsulation o

75 rporated an alkyne-containing amino acid for click chemistry, an important post-translationally modif

76 tic acid into cellular proteins coupled with click chemistry and (ii) detecting a specific protein of

77 Using click chemistry and a modified antibody array to detect

78 proteins, allowing for their detection using click chemistry and antibody arrays.

79 lly conserved compounds that are amenable to click chemistry and can be used as molecular probes in v

80 igh sensitivity and spatial resolution using click chemistry and fluorescence microscopy.

81 minescent quantum dot (QD) surfaces that use click chemistry and hydrazone ligation under catalyst-fr

82 We adapted newly developed procedures for click chemistry and iPOND (Isolation of proteins on nasc

83 ace N-glycoproteins by combining copper-free click chemistry and MS-based proteomics.

84 Herein, an introduction to the impact of click chemistry and other bioorthogonal reactions on the

85 2,3-triazole moieties were prepared by using click chemistry and showed low nanomolar inhibitory acti

86 backbone using for the first time sequential click chemistry and stepwise assembly of functional buil

87 lyl donor/acceptor unnatural nucleosides via click chemistry and studies on the duplex stabilization

88 njugated with two oligoguanosine strands via click chemistry and the conjugates were then self-assemb

89 , we present an optimized strategy combining click chemistry and the genetic encoding of unnatural am

90 ibrary of these compounds was prepared using click chemistry and the selectivity concept was validate

91 the utility and multimodal possibilities of click chemistry and to increase enzyme active site inclu

92 ique combination of facile accessibility via click chemistry and truly diverse supramolecular interac

93 Whole cells are labelled via click chemistry and visualized using super-resolution mi

94 ole-based IL moieties were synthesized using click chemistry and were further copolymerized with laur

95 triazole linker is afforded by azide-alkyne "click" chemistry and comprises the third arm of the trip

96 eudomonas aeruginosa were synthesized using "Click" chemistry and DNA chemistry.

97 h strategy using a combination of thiol-ene "click" chemistry and traditional esterification reaction

98 tylene group which can be functionalized via click chemistry, and the other is an amine group which c

99 ates from the stoppering reaction, based on "click" chemistry, and thus from the presence of two tria

100 any different biological purposes, including click chemistry applications, diversity-oriented synthes

101 The oligoproline/click chemistry approach holds great promise for the pre

102 The in situ click chemistry approach to lead discovery employs the b

103 The target-guided, in situ click chemistry approach to lead discovery has been succ

104 Hence, the click chemistry approach with an in situ template of a r

105 Herein, we report for the first time a "click" chemistry approach to oligonucleotide probe elong

106 taining azide (-N3) functionalities using a "click" chemistry approach.

107 Herein, we describe a "click chemistry" approach for the synthesis of PROTACs.

108 These studies establish Cu-free click chemistry as a bioorthogonal reaction that can be

109 inal alkyne group, we were able to visualize click chemistry at the single-molecule level, which reve

110 The click chemistry based on the addition of photochemically

111 Here, we have used a click chemistry based strategy to introduce six pairs of

112 fluoride exchange (SuFEx) is a new family of click chemistry based transformations that enable the sy

113 Herein, we report a click-chemistry based approach to the synthesis of HDACi

114 We recently reported a click-chemistry based method for generating RNAseq libra

115 eloped a robust, high-throughput compatible, click chemistry-based approach to identify small molecul

116 Here we describe a click chemistry-based approach, using an azido derivativ

117 transformation into acyl-CoAs and subsequent click chemistry-based detection, to demonstrate that zDH

118 sing a combination of metabolic labeling and click chemistry-based mass tagging.

119 rovide insight for the future development of click chemistry-based photoreactive probes for the in si

120 This click chemistry-based strategy for ABPP represents a uni

121 This letter presents a click-chemistry-based assay for proteins (CAP) that allo

122 sulfonates can be further functionalized via click-chemistry-based post-polymerization modification.

123 ith the ART probe can then be isolated using click chemistry before identification by liquid chromato

124 dy against respiratory syncytial virus using click chemistry between biotin molecules functionalized

125 METHODS AND Using crosslinking analogs, click chemistry, binding assays, and functional assays,

126 "Click" chemistry bioconjugation using a poly(ethylene gl

127 ecial focus capitalizes on the impact of the click chemistry concept on dendrimer synthesis and the p

128 ys a series of steps that capture fully the "click" chemistry concepts that have greatly facilitated

129 e family, and (2) terminal alkyne groups for click chemistry conjugation of reporter tags.

130 protein targets and has an alkyne handle for click chemistry conjugation to reporter tags.

131 Click chemistry, copper(I)-catalyzed azide-alkyne cycloa

132 Assisted with click-chemistry coupling, we sandwiched one G-quadruplex

133 (68)Ga-aquibeprin was obtained by click-chemistry (CuAAC) trimerization of a alpha5beta1 i

134 techniques, including acyl-biotin exchange, click chemistry, cysteine mutagenesis, and mass spectrom

135 ) linker was found compatible with different click chemistries, demonstrated in bioreversible protein

136 Click chemistry describes a family of modular, efficient

137 orthogonal amino acid using copper-catalyzed click chemistry, either before or after the silk fibers

138 gamma-azido vinyl sulfones, and azide-alkyne click chemistry enabled the synthesis of vinyl sulfone-b

139 click reaction is orthogonal to azide-alkyne click chemistry, enabling sequential photoclick/azide-cl

140 uorophore conjugates by means of copper-free click chemistry, enabling the visualization of glycans i

141 bstitutes for high-throughput screening, and click chemistry ensures that chemical synthesis is fast,

142 We specifically focus on click chemistry, enzymatic ligation, and affinity bindin

143 Biotin conjugation to EdU-labeled DNA using click chemistry facilitates a single-step streptavidin p

144 various thiols by radical mediated thiol-ene click chemistry, followed by self-assembly in deionized

145 or D-glucose to L-cysteine using thiol-ene "click" chemistry, followed by their conversion to the co

146 al reagents that enable protein labeling via click chemistry for affinity purification and detection

147 derived components and relied on copper-free click chemistry for bioorthogonal covalent cross-links t

148 nation atoms were prepared in high yield via click chemistry for potential incorporation into metal c

149 We report a new type of azide-alkyne click chemistry for the synthesis of protein conjugates

150 of biomolecules via Huisgen cycloaddition ("click chemistry") for positron emission tomography (PET)

151 hat can undergo bio-orthogonal ligation via 'click' chemistry, for example, an azide, and can be used

152 des and cyclooctynes, also known as "Cu-free click chemistry," for labeling biomolecules in live mice

153 mizing reaction conditions, we converted CB6 click chemistry from a rotaxane synthesis tool into a us

154 ification of target proteins, an alkyne as a click-chemistry handle for target identification, and a

155 Click chemistry has become a ubiquitous chemical tool wi

156 and kinetics of radical initiated thiol-ene click chemistry has been studied computationally at the

157 labeling using sugar analogs compatible with click chemistry has the potential to provide new insight

158 Several forms of click chemistry have previously been used to modify poly

159 Assisted by click chemistry, herein, we attached pulling handles via

160 eling of target proteins and biologics using click chemistries, (ii) for glycoengineering of antibodi

161 tocol and the labeling of DNA-Pt by means of click chemistry in cells.

162 he successful advancement of Cu(I)-catalyzed click chemistry in glycoscience and its applications as

163 eagents should expand the use of copper-free click chemistry in the hands of biologists.

164 and evaluated a pretargeting strategy using click chemistry in vivo to reduce kidney uptake and avoi

165 cles and catenanes has been developed using "click" chemistry in combination with Sauvage's metal tem

166 meter and synthesized via an astoichiometric click-chemistry in-emulsion method, controllably display

167 in one case and through a triazole linkage ('click chemistry') in the other.

168 les for sulfur(VI) fluoride exchange (SuFEx) click chemistry, in which either the alkenyl moiety or t

169 nality onto solid surfaces using well-known "click" chemistry involving easy-to-react linkers.

170 labeling followed by the use of reagent-free click chemistry is an established technique for in vitro

171 Target-guided synthesis by in situ click chemistry is combined with synthetic epitope targe

172 Thiol-yne click chemistry is demonstrated as a modular platform fo

173 The use of thiol-ene click chemistry is demonstrated for the first time as a

174 ds and their subsequent macrocyclization via Click chemistry is described.

175 osphors and polymer matrices via Diels-Alder click chemistry is devised as a method.

176 Strain-promoted "click" chemistry is used to post-synthetically modify th

177 ctynes with azides, also called "copper-free click chemistry", is a bioorthogonal reaction with wides

178 2] cycloaddition, this method, often termed "click chemistry", is currently incompatible with living

179 as well as their myriad of applications in "click" chemistry it is interesting to note that the synt

180 We present the use of a new form of click chemistry known as SuFEx (sulfur(VI) fluoride exch

181 review will focus on recent applications of click chemistry ligations in the preparation of imaging

182 As with most of click chemistry, many essential features of sulfur(VI) f

183 A two-part, click-chemistry mediated activity-based probe, N-but-3-y

184 also included an alkyne group to facilitate click chemistry-mediated conjugation of reporter tags fo

185 e analogue UV cross-linked and visualized by click-chemistry-mediated fluorescent labeling.

186 Here we describe the application of a new click chemistry method for fluorescent tracking of prote

187 inking of BT to a biotin-linked moiety using click chemistry methods and coating of BT with nonreacti

188 tion, controlled radical polymerization and "Click Chemistry" methods have significantly evolved over

189 tein nanocapsules incorporating copper-free "click chemistry" moieties, polyethylene glycol (PEG) uni

190 tion (also known as copper-free azide-alkyne click chemistry), nanoparticles bearing a high-density s

191 Click chemistry of alkyne-modified RNA with different re

192 Ruthenium catalyzed 1,3-cycloaddition (click chemistry) of an azido moiety installed on dihydro

193 The cyclopropenone-based phototriggered click chemistry offers exciting opportunities to label l

194 d utilizing carbonate ester and azide-alkyne click chemistries on the surface of the closo-B(12)(2-)

195 t of various polymers by using silane-based "click" chemistry on silica surfaces and within glass mic

196 ent probe cyanine 5 at their focal point via click chemistry permitted the evaluation of their cellul

197 Click chemistry plays a dual role in the design of new c

198 acid (17-ODYA) can serve as a bioorthogonal, click chemistry probe for in situ labeling, identificati

199 Additionally, in silico modeling of click-chemistry products may prove useful in rational dr

200 phthalocyanines was attained by means of a "click" chemistry protocol.

201 Modifying Janus particles through thiol-yne click chemistry provides a fast-reacting, scalable synth

202 Click chemistry provides extremely selective and orthogo

203 tein of interest with high sensitivity using click chemistry, proximity ligation and fluorescence mic

204 tion sequencing) library synthesis that uses click chemistry rather than enzymatic reactions for the

205 4-5-step process, involving as a key step a click chemistry reaction between ynamides and azides.

206 alyzed azide-alkyne cycloaddition (CuAAC), a click chemistry reaction, serves as an approach to struc

207 By taking advantage of a facile, one-pot click chemistry reaction, we report herein the preparati

208 ogs followed by a fluorescent azide-coupling click-chemistry reaction.

209 Two click chemistry reactions were now combined to form nucl

210 ed AutoClickChem, capable of performing many click-chemistry reactions in silico.

211 were used as reaction templates for in situ click-chemistry reactions to generate a congeneric serie

212 t supramolecular catalysts for alkyne-azide "click chemistry" reactions, yielding the desired 1,4-add

213 1,4-substituted triazole moiety prepared via click chemistry resulted in a significant bathochromic s

214 exciting reaction to other well-established click chemistry schemes, its high reaction speed and its

215 Though both in silico screening and click chemistry seek to make drug discovery more feasibl

216 dendrimers synthesized from Cu(I)-catalyzed click chemistry showed a high level of copper contaminat

217 Use of the propargyl ether 44 and Click chemistry showed that niphatenone B binds covalent

218 ) azide-alkyne cycloaddition, an element of "click chemistry," stationary phases carrying long alkyl

219 uromutilin derivatives were synthesized by a click chemistry strategy.

220 te a carbohydrate array via efficient local "click" chemistry strategy.

221 vantages of highly chemo- and regioselective click chemistry, such as mild reaction conditions, effic

222 unched AChE as a reaction vessel for in situ click-chemistry synthesis of high-affinity TZ2PA6 and TZ

223 e in situ reaction products established that click-chemistry synthesis with surrogate receptor templa

224 uses a novel synthetic photoactivatable and click chemistry-taggable phospholipid probe, which, when

225 In the subsequent click chemistry tagging reaction, azide-labeled proteins

226 We employ an efficient "click chemistry" technique to synthesize aptamer-polymer

227 by a combination of e-beam lithography and "click" chemistry techniques.

228 novel class of DIFO reagents for copper-free click chemistry that are considerably more synthetically

229 Here, we report a Cu-free variant of click chemistry that can label these biomolecules rapidl

230 of efficient blue-emitting fluorophores via click chemistry that could be potential luminophores in

231 We confirmed using 5-ethynyl-2'-deoxyuridine click chemistry that the Oc-Cre lineage includes very fe

232 ent of selective bioorthogonal reactions or "click" chemistry that can proceed in live animals.

233 abolic labelling combined with bioorthogonal click chemistry (that is, reactions performed in living

234 eactions have been developed, exemplified by click chemistry, that enable the efficient formation of

235 e caged dye to the substrate of interest via click chemistry, the allyloxy appendage was functionaliz

236 includes discussion of the philosophy behind click chemistry, the details and benefits of bioorthogon

237 re constructed according to the reactions of click chemistry, they can be easily synthesized for subs

238 Using copper-assisted click chemistry, they can be functionalized with a varie

239 re, we introduce several approaches based on click chemistry, through which we study the distribution

240 r cells and perform efficient, biocompatible click chemistry, thus acting as intracellular nanoscale

241 enyl)ethanols reacted with alkynes employing click chemistry to afford high yields of optically pure

242 ve developed a modification of bioorthogonal click chemistry to assay the palmitoylation of cellular

243 Here, we successfully use click chemistry to construct DNA templates for sgRNA exp

244 l coupling of multifunctional monomers using click chemistry to create a branched cross-linked polyme

245 C termini using sortase transpeptidation and click chemistry to create a covalently linked IgG antibo

246 zed peptides were synthesized by solid phase click chemistry to develop novel, potent, selective MC4R

247 ic modification of PG can be extended to use click chemistry to fluorescently label the mature PG in

248 polymer backbone by exploiting azide-alkyne click chemistry to functionalize the hydrogels with a pe

249 e picture) were subjected to high-throughput click chemistry to generate a library of sialic acid ana

250 e cell labelling with azidomyristic acid and click chemistry to identify N-myristoylated proteins in

251 ligosaccharide engineering and bioorthogonal click chemistry to label various commensal anaerobes, in

252 olyprotein construct and DNA via copper-free click chemistry to PEG-coated substrates and a strong bu

253 Here we use click chemistry to show that derivatives of two such com

254 canonical amino acid tagging method, we used click chemistry to study the role of protein synthesis a

255 lkynylphosphonate can be functionalized via "Click" chemistry to generate the 1,2,3-triazolyl or subs

256 Here, we present a simple strategy using 'click' chemistry to couple biotin to a 'caged' photoclea

257 ere surface functionalized with folate using click-chemistry to improve targeted uptake by the malign

258 Using "click chemistry" to detect 5-ethynyl-2'-deoxyuridine (Ed

259 to activate cellulosic materials for CuAAC "click chemistry", to develop new fluorogenic esterase se

260 = 4-30 nM), were conveniently assembled by "click chemistry." Two radioiodinated probes, [125I]10a a

261 active scaffolds was synthesized by means of click-chemistry under non-conventional microwave heating

262 Click chemistry was employed to cross-link peptides to D

263 Stepwise click chemistry was employed to introduce cross-links at

264 Click chemistry was then used for the highly efficient a

265 Total synthesis combined with click chemistry was used to label the Ts1 protein molecu

266 A Huisgen 1,3-dipolar cycloaddition "click chemistry" was employed to immobilize azido sugars

267 "Click chemistry" was used to conjugate alkyne-containing

268 discovered by combinatorial copper-catalyzed click chemistry, was synthesized in situ by incubating e

269 ighly DNA specific and efficient enzyme, and click chemistry, we demonstrated that as little as 0.1 f

270 in dynamic combinatorial chemistry (DCC) or click chemistry were explored.

271 can be covalently attached to surfaces using click chemistry, where they retain their abilities to in

272 he Cu(I)-catalyzed [3 + 2] cycloaddition, or click chemistry, which allows rapid, versatile, and spec

273 iminary results strongly suggest that use of click chemistry, which led to a 1,4-diphenyltriazole-bas

274 can be employed for toxin derivatization by click chemistry with an azide-containing reporter molecu

275 g in a crude membrane fraction, followed by "click chemistry" with a rhodamine-azide reporter, enable

276 ycloaddition reaction (often referred to as "click" chemistry) with fluorescent azides, followed by m

277 t of the ligand to a surface by conventional click chemistry without disturbing selective interaction

278 lysis of ~ 300 derivatives synthesized using click chemistry yielded compounds with greatly enhanced

279 of new biotin 1,2,3-triazole analogues using click chemistry yielded our most potent structure (K(i)