ライフサイエンスコーパス: clone

1 chemotherapy targeting the underlying B-cell clone.

2 as caused by a serogroup B sequence type 269 clone.

3 ay contribute to increased virulence in this clone.

4 otent endocrine and ducto-endocrine bipotent clones.

5 mplete neutralization phenotype of these T/F clones.

6 thermore activate primary KIR2DS1(+) NK cell clones.

7 retion among Ag-stimulated low-affinity iNKT clones.

8 oma plasma cell clones than MGUS plasma cell clones.

9 s from a total of 7,300 TRPV1 random mutant clones.

10 lized and screened to identify DENV-specific clones.

11 ased methods for partitioning sequences into clones.

12 e secretion than "ineffective" CD8(+) T-cell clones.

13 the expense of the number of replicate hiPSC clones.

14 tability is shared among a limited number of clones.

15 o be only distantly related to recent USA300 clones.

16 nsistent with regional outgrowth of advanced clones.

17 ence and transmission in the most successful clones.

18 lonal, consisting of tens to hundreds of LPC clones.

19 TOP-mediated editing in cell populations and clones.

20 t populations (10 of 14) were founded by few clones.

21 ring was particularly evident among expanded clones.

22 ent levels of responses within the M1 and M2 clones.

23 the skeletons of Dolly and her contemporary clones.

24 matic hypermutation, and selection of B cell clones.

25 ion and the emergence of treatment-resistant clones.

26 m recurrent tumors are seeded from different clones.

27 ted in-solution, and introduced to molecular clones.

28 y the madC gene was identified by positional cloning.

29 stained with monoclonal anti-PSMA antibody (clone 3E6, 1:100, M3620).

30 also evaluated an antibody specific for XPF (clone 3F2).

31 al, polyreactive natural antibodies, the IgM clone 9H4, revealed its ability to recognize the interme

32 Here, we have identified and cloned a pollen-expressed P. rhoeas threonine-aspartate-

33 l lines that resulted in long-term surviving clones acquiring the ALT pathway but at a very low frequ

34 All cloned AHAs could restore immune effector functions to p

35 Most of the new clones, all already provided with a genetic and sanitary

36 resence of 46B8, we isolated three resistant clones, all harbouring the same mutation (Ser301Phe) in

37 ition, compounds that acted as agonists at a cloned alpha-like BiOctR also induced a hyperactivity re

38 hosphorylation with saracatinib in these CTL clones also severely compromised their functional activi

39 In this study, we cloned an isopentenyl pyrophosphate isomerase (IPPI) cDN

40 he coexistence of an active multiple myeloma clone and a benign MGUS clone, and thus provides a uniqu

41 mphotropic HCV, we established an infectious clone and chimeric virus of hepatotropic and lymphotropi

42 GCs became independent of the initial clone and evolved toward dominance of individual clonal

43 mplified by employing the genome sequence to clone and functionally characterize the promoters in a p

44 munoglobulin sequences and 5' and 3' RACE to clone and sequence heavy and light chain immunoglobulin

45 Here, we describe a set of tools to rapidly clone and stratify thousands of cancer mutations at base

46 We cloned and analyzed the expression of the genes Emx1, Lh

47 Here we cloned and characterized rare affinity-matured human NAN

48 igh throughput methods to sequence 18S rRNA, cloned and sequenced 28S rRNA and microscopically counte

49 These viruses were also cloned and sequenced through Sanger sequencing to confir

50 aspase genes were identified, the cDNAs were cloned and the recombinant enzymes were obtained after t

51 bers of clonotypes, including newly detected clones and a decline in overall T-cell clonality.

52 to separate contributions of multiple tumor clones and assorted stromal and infiltrating cell popula

53 the sensory analysis of the oils from these clones and compared them with the oils from the respecti

54 K-inactivating) alterations in all resistant clones and determined that ADK is required to phosphoryl

55 te partitioning of sequence data into B-cell clones and identification of the starting point of a B-c

56 ng data that clusters somatic mutations into clones and infers a phylogenetic tree that describes the

57 Using this approach, we expanded minor clones and obtained epithelial cell-specific DNA/RNA for

58 because both natural IL-26 released by Th17 clones and rhIL-26 lacked antimicrobial potency against

59 ext generated PH and PA strain-specific Th17 clones and show that P. acnes strains induce Th17 cells

60 icult, potentially due to the rarity of bNAb clones and their precursors.

61 tudy, we examined two well-studied molecular clones and two transmitted/founder (T/F) clones for thei

62 netic component, and so far, only positional cloning and genomewide association studies have been use

63 computational effort between regions through cloning and merging steps, as in the weighted ensemble m

64 Previous analyses of these methods relied on cloning and Sanger sequencing to evaluate their efficien

65 iously from the same samples by standard PCR cloning and Sanger sequencing, and showed that both meth

66 It progresses from genetics to cloning and sequencing to biochemistry to structural bio

67 twins to investigate how genetic background, clone, and passage number contribute.

68 ive multiple myeloma clone and a benign MGUS clone, and thus provides a unique model to assess the re

69 The majority of antibodies that we isolated, cloned, and sequenced belonged to the IgM isotype of the

70 In 1 patient, the CD19(-) clone appeared as a late relapse 19 months after complet

71 In cancer, malignant clones are characterized by recurrent somatic mutations

72 VH4-34-expressing clones are common in the naive B cell repertoire but are

73 rations that affect whether MGUS plasma cell clones are responsive to anti-multiple myeloma therapy.

74 s with "matched" heavy and light chains were cloned as IgG1, and those of high affinity for specific

75 ce-based haplotype maps using diploid potato clones as parents.

76 r lasting genomic differences between stable clones as revealed by DNA arrays and sequencing.

77 ST283 is a zoonotic GBS clone associated with farmed freshwater fish, capable of

78 ckade increased the size of subdominant TCD8 clones at the peak of their primary response, and it als

79 applied SeqCNV to both bacterial artificial clone (BAC) and human patient NGS data to identify CNVs.

80 with a diverse and expanded group of B cell clones bearing a wide range of antibody affinities.

81 Clones bearing the conditional knockout cassette are rec

82 We have cloned both; while highly conserved in domain organizati

83 A second C5a receptor, C5L2, has also been cloned but has received much less attention because its

84 a virtually unique IgH locus in every B cell clone by intrachromosomal recombination between two swit

85 is stochastic variability is evident between clones, by RNA-sequencing, and at the single-cell level,

86 LL progression and suggest that the leukemic clone can generate an autoactivation loop through S100-A

87 A plaque-purified MERSMA clone caused weight loss and fatal infection.

88 While genome-wide TF ORFeome clone collections are increasingly available, screening

89 d provide proof of principle for therapeutic cloning combined with cell therapy.

90 ne mapping of quantitative traits, map-based cloning, comparative mapping, and in marker-assisted whe

91 from an effective and one of two ineffective clones conferred upon primary CD8(+) T cells the equival

92 cted quantitative expansion of T cell-biased clones consistent with an adaptive immune response.

93 Each clone consists of the progeny of a single progenitor cel

94 ssues, and absolute numbers of unique T-cell clones correlated with respective T-cell counts.

95 cing, and was immune to wild-type infectious clones corresponding to CaLCuV or Beet curly top virus (

96 n A2-specific Vdelta2(neg) gammadelta T-cell clones could be derived from peripheral blood mononuclea

97 B-4C use for controlling outbreaks caused by clones covered by the vaccine.

98 ice were superinfected with a VlsE-deficient clone (DeltaVlsE) at day 28 p.i., the active anti-B. bur

99 n (35.5 Mb) and three benznidazole-resistant clones derived from a single drug-selected population.

100 ans, obtained by sequencing single cells and clones derived from primary fibroblasts, which allows us

101 Infectious clone-derived virus initiated infection and transmission

102 d common ancestor knock-in mice Env(+)B cell clones develop anergy and partial deletion at the transi

103 Notably, GI tract clones display extensive sharing of sequence variants am

104 KK10-stimulated "effective" CD8(+) T-cell clones displayed significantly more rapid TCR signal pro

105 The longevity of both the donor and the cloned dog was close to the median lifespan of Afghan ho

106 inally, IL-10-producing drug-specific T cell clones downregulated the response of autologous effector

107 racing of Prox1(+) cells revealed long-lived clones during homeostasis and after radiation-induced in

108 on behaviors and three control subjects, two clones each.

109 d apoptotic status of bovine preimplantation cloned embryos.

110 ing to developmental failure in somatic cell cloned embryos.

111 ctive and potent CDK4/6 inhibitor LY2835219, clones emerged and several were found to harbor amplific

112 reased yield of antibody-producing hybridoma clones, ensured monoclonality of sorted cells, and reduc

113 From a CD8(+) T cell clone established by stimulation of HLA-A2(+) CD8(+) T c

114 ransfer of MPO431-439-specific CD8(+) T cell clones exacerbated disease mediated by MPO-specific CD4(

115 Finally, the resistant clones exhibit cross-resistance with oxaliplatin but not

116 One of the two EMT-positive clones exhibited aggressive and stem cell-like character

117 CREBBP-mutant DLBCL clones exhibited reduced histone H3 acetylation, express

118 erived from activated PH and not PA-specific clones exhibited robust bactericidal activity against P.

119 luble antigen-binding fragments (Fab), these clones expressed well, were predominantly monomeric and

120 Here, we cloned, expressed, and characterized the GTPase LdSar1 a

121 the F-MuLV envelope protein is dominated by clones expressing a Valpha2 gene segment, thus allowing

122 Piperacillin-responsive skin-homing CD4(+) clones expressing an array of Vbeta receptors were activ

123 Our results demonstrate a selective loss of clones expressing high-affinity iNKT-TCRs from the iNKT

124 (HSPC) compartment and interrogated dominant clones for MDS-initiating cells.

125 d efficient selection of low-affinity B cell clones for proliferative clonal expansion.

126 rate that the clonal stability and number of clones for the CTL response against an epitope are inver

127 lar clones and two transmitted/founder (T/F) clones for their sensitivities to a panel of bNAbs in ce

128 We identified and cloned four AQPs from C. lectularius, assessed tissue an

129 re, we sequenced the genomes of one endpoint clone from each population to test whether the history o

130 IgG AHAs cloned from a single human donor exhibited restricted sp

131 However, TCRs cloned from an effective and one of two ineffective clon

132 tylserotonin-O-methyltransferase (ASMT), was cloned from apple rootstock, Malus zumi.

133 lum, and although alpha-like OctRs have been cloned from Balanus improvisus (BiOctR) and Drosophila m

134 ear transfer to generate three lines of mice cloned from iNKT nuclei.

135 A type 2 metallothionein gene, SsMT2, was cloned from Suaeda salsa, a salt- and alkali-tolerant pl

136 The HCV1406 TCR was cloned from T cells that expanded when a hepatitis C vir

137 quence identity with a proteinase previously cloned from the American cockroach (Per a 10).

138 re found in CD247 complementary DNAs (cDNAs) cloned from the patient as well as in cDNA and genomic D

139 raniol into the secologanin pathway was also cloned from the trancriptome resource.

140 In this study, we identify 21 fosmid clones from a human gut microbiome metagenomic library t

141 About half of the generated T-cell clones from children and adults reacted to unknown epito

142 s method was applied to the evaluation of 50 clones from five mAbs programs, allowing for identificat

143 l enzymes, we have screened over one million clones from metagenome DNA libraries derived from sixtee

144 e, we demonstrated that MiHA-specific T cell clones from patients with selective GVL reactivity showe

145 ognized C-peptide amino acids 19-35, and two clones from separate donors responded to insulin B-chain

146 Therefore, clones from the Haplobank combined with the use of rever

147 CMDA-amplified single cells with unamplified clones from the same population, we validated the proced

148 Expanded clones from two T1D subjects recognized distinct IGRP pe

149 of advanced technologies, including ordered-clone genome sequencing, whole-genome shotgun sequencing

150 f RANK/RANKL loop activation in the leukemic clone, given recent reports on its role in CLL progressi

151 The number of dominant clones (>/=10% frequency) in a tumor correlated strongly

152 Certain clones harboring ciHHV-6A/B spontaneously express viral

153 Nef clones harboring nonconsensus variants at codon 9 downre

154 HDR with more than 80% of the selected WA09 clones harboring the transgene inserted at the targeted

155 Animal cloning has gained popularity as a method to produce gen

156 remained unchanged, distinct A:cc5 epidemic clones have nevertheless emerged.

157 The mutant clones identified were too large to be accounted for sol

158 hain reaction was used to map the neoplastic clone in 20 adults with LCH, ECD, and HCL.

159 rom expansion of a transduced natural killer clone in response to chronic Epstein-Barr virus viremia.

160 cial in drug trials and to follow individual clones in epidemiological studies, and to understand how

161 ference between the proportion of responding clones in M1 (19 [95%]) and M2 (15 [75%], p=0.13).

162 y less MHCII, and grew faster than wild-type clones in s.c. and orthotopic xenograft models.

163 frequency, i.e. the proportion of resistant clones in the parasite population at each location, and

164 TCR repertoire revealed expansion of T cell clones in the setting of neoantigen loss.

165 tem in preventing the outgrowth of aneuploid clones in tissue culture.

166 Nosocomial clones, including epidemic sequence type 258 (ST258), ha

167 Streptococcus sanguinis, we have developed a cloning-independent methodology, which uses a countersel

168 onal hits (2nd Hit) transforms the expanding clone into cancer.

169 omain from the collagen alpha3(IV) chain was cloned into a mammalian expression vector, pCI-neo, with

170 l receptor (TCR) alpha- and beta-chains were cloned into a retroviral vector.

171 he main factors limiting entry of new B cell clones into ongoing immunization-triggered GC responses.

172 Each clone is the progeny of a single B cell responding to Ag

173 TPA cloning is scarless and sequence independent.

174 een assembled together by modular idempotent cloning, it is unclear if such simplified strategies sca

175 ere generated from a genotype 2a-derived HCV clone (Jc1Gluc2A) by site-directed mutagenesis.

176 igene expression due to increased labour for cloning, limited vector capacity, requirement of multipl

177 tely enables the emergence of drug-resistant clones, limiting the long-term effectiveness of these th

178 In these clones, lysosomal pH was increased and the proteolytic a

179 We confirm that across clones MAE status correlates with expression level, and

180 We found that clones mainly accumulate mutations in a linear successio

181 le from PMEZ DNA and all PCR generated phnJL clones matched those of the Pseudomonas sp.

182 Our results therefore suggest that different clones may have adopted different strategies to overcome

183 f cells and organisms, relying upon cellular cloning methods that remain unchanged for decades, are l

184 ase resistance (R) genes were identified and cloned more than two decades ago.

185 ts of the analysis showed that the fruits of clone no.

186 Analysis of cloned Ntcp from all species revealed a pronounced role

187 line with this, a highly HBV permissive cell clone of HepAD38 cells showed a prominent association of

188 ctor mutations tend to occur in the founding clone of myeloid cancers, and these mutations have recen

189 There are currently no molecular clones of a New World ZIKV available that lack significa

190 t and track the emergence and spread of MRSA clones of human importance.

191 and populations, this study will support the cloning of a major yield QTL on chromosome 3B that is hi

192 It has been 20 years since the cloning of ATR, the last of the three to be identified.

193 rminants in the blast fungus resulted in the cloning of avirulence genes PWT3 and PWT4, whose gene pr

194 sed significant progress in fine-mapping and cloning of genes controlling QDR.

195 We report here the cloning of Male Sterile23 (Ms23), encoding an anther-spe

196 The cloning of Ms2 has substantial potential to assemble pra

197 Cloning of multiple genes in a single vector has greatly

198 Here we report cloning of the biosynthetic gene cluster for the beta-la

199 Here, we report the identification and cloning of the M. truncatula NFS2 gene that regulates th

200 We herein report the cloning of the Medicago truncatula NFS1 gene that regula

201 Here, we describe the map-based cloning of the Ms2 gene and show that Ms2 confers male s

202 Here, we report cloning of ZmNope1 on the basis of synteny with rice.

203 n) was tested against the FDA-approved SP263 clone on biopsied patient tissues.

204 loss of high-quality and MUC16 neoantigenic clones on metastatic progression, suggesting neoantigen

205 We found that two recurrent Treg cell clones, one prevalent in prostate tumors and the other a

206 ts in the emergence of resistant cancer cell clones only in the presence of IFN-gamma within the tumo

207 In all cases, the resistant clones originated prior to 2010, indicating that PNSP ar

208 These clones possess 100-140 fold enhanced catalytic efficienc

209 demonstrate that public, as well as private, clones possess predictable high-dimensional immunogenomi

210 e identity of the begomoviruses and that all clones possessed a full complement of the TrAP gene.

211 nces) and was sufficiently robust for public clone prediction across individuals and studies prepared

212 f tumor detection, with only 1 to 4 dominant clones present at >/=10% frequency.

213 e at present dominated by descendants of NVT clones present before vaccination.

214 Antibiotic-resistant clones rapidly emerge mainly by acquisition of antibioti

215 s of effective and ineffective CD8(+) T-cell clones recognizing the identical HLA-B*2705-restricted H

216 c cluster analysis of multiple CD8(+) T-cell clones recognizing the identical HLA-B*2705-restricted H

217 ctors that influence the diversity of B cell clones recruited into GCs are unclear.

218 odon of two C-terminal ORFs in an infectious clone reduced virus yield.

219 sease and emergence of a multidrug-resistant clone reported in this issue of the Journal of Clinical

220 Recognition by the CD8(+) T cell clone required N-terminal O-linked mannosylation of MPT3

221 alance this advantage, selecting for a minor clone resistant to chemotherapy.

222 fy how MGUS and multiple myeloma plasma cell clones responded to anti-multiple myeloma therapy in pat

223 Analysis of MR1T cell clones revealed specificity for distinct cell-derived an

224 Analysis of 14 of these clones revealed that they all share a fragment of DNA wi

225 Here, a single cell cloning revealed that HepAD38 cells, a widely-used HBV-i

226 Using molecular cloning, RT-PCR, Western blotting, immunolocalization an

227 ure, which has been stably maintained in all clone SA isolates derived from various hosts and times.

228 Sequence-based analyses revealed that clone SA possesses a unique CPS locus with a mosaic stru

229 We cloned sabaeus CD4 and 10 candidate coreceptors.

230 A recombinant Asd(+) plasmid pCZ1 with the cloned Salmonella Choleraesuis O-antigen gene cluster wa

231 polyclonal seeding, in which two independent clones seeded the metastatic liver tumor after having di

232 Effective and ineffective CD8(+) T-cell clones segregated based on responses to HIV-1-infected a

233 pared the results with those obtained by PCR-cloning-sequencing (PCR-CS).

234 recent report detailing the health status of cloned sheep concluded that the animals had aged normall

235 urally conceived sheep, and our healthy aged cloned sheep.

236 and functional characterization of MR1T cell clones showed multiple chemokine receptor expression pro

237 with integrations near MECOM and LMO2 These clones showed stable behavior over multiple years and ne

238 of medical interest, it has eluded molecular cloning since its discovery, and the gene that codes for

239 ndary separating activated and non-activated clones, so to characterize the selectivity and specifici

240 sampled populations, diluting the signals of clone-specific aberrations, and complicating estimation

241 We further found that clone-specific effects play a strong role in recurrent a

242 aberrations, and complicating estimation of clone-specific genotypes.

243 Parasite clone-specific growth was then analysed in co-culture as

244 We also developed a lentiviral vector and cloning strategy to generate high-complexity pooled dual

245 es and five genes supported by previous gene cloning studies in maize, rice, and Arabidopsis.

246 , and the concerning international high-risk clones (such as ST111, ST175, and ST235) still needs to

247 Phenotypic analyses of Trl and Atro clones suggest that Atro is required to modulate the tra

248 To determine MGUS clone susceptibly to therapy, future studies might seek

249 We generated a two-plasmid infectious clone system from which infectious virus was rescued tha

250 esponse against multiple myeloma plasma cell clones than MGUS plasma cell clones.

251 the same Hia "strain" belonging to the ST23 clone that has previously been reported only outside Eur

252 Finally, we identified CF specific clones that correlate highly with sweat chloride test, B

253 We also identify rare 'outlier' clones that deviate from these dynamics, and further sho

254 tumor heterogeneity, they were restricted to clones that were anatomically distant from the biopsy si

255 vivo expansion of neoantigen-specific T cell clones that were reactive to mutant neopeptides found in

256 ere we show, using B6/Cast hybrid pre-B-cell clones, that a limited number of V segments on each alle

257 Therefore, we cloned the maize Tel2 and Tti1 homologs and showed that

258 Cloning the sigma2 receptor resolves a longstanding myst

259 cal properties differ significantly from one clone to another in terms of virulence and host invasive

260 fever (CHIKF) pandemic, we used an EILV cDNA clone to design a chimeric virus containing the chikungu

261 and crop species, and although none has been cloned to date, transcript profiling experiments have id

262 uations reveal haematopoiesis from these iPS clones to be indistinguishable from that associating wit

263 ant TCD8 are more likely than immunodominant clones to escape tolerance mechanisms and may contribute

264 ue model to assess the responses of separate clones to the same anti-multiple myeloma therapy, in the

265 We employed molecular cloning to examine how PTEN's stability, subcellular loc

266 nonetheless reversible for wild-type and sub-cloned U2OS cells, except for lasting genomic difference

267 y was not because of the resampling of large clones, underscoring the feasibility and relevance of un

268 followed the maturation of stem cell-derived clones using sparse lineage tracing in the regenerating

269 In monoclonal cases, the dominant clone varied between 11% and 99% of TCRbeta transcripts.

270 posited from a fusion mixture as single-cell clones via FACS.

271 SRK-positive Bacterial Artificial Chromosome clone was found to contain complete SRK and SCR sequence

272 focused TCR-peptide binding, the ILA1 T-cell clone was still cross-reactive.

273 Using map-based cloning we positioned the her2 mutation to the At5g63620

274 Using map-based cloning, we delimited a candidate region including two l

275 By using single-cell cloning, we identified genes that are associated with hi

276 70 most frequent putative pathogenic T cell clones were alphabeta T cells.

277 First, clones were asymmetrically distributed between different

278 HIV-1 neutralization, and anti-H2A antibody clones were found to neutralize HIV-1.

279 Small T-cell clones were often observed in T- and NK-cell tumors in a

280 eover, many, and partly very large, expanded clones were seen predominantly among IgM(+) memory B cel

281 The predominant clones were sequence type [ST] 22 (n = 183; 47.8%), ST45

282 Most (87%) top expanded lesional T-cell clones were shared with nonlesional tissues, and they we

283 KRAS-mutated lung cancer cell clones were stably silenced for LSD1 expression.

284 opathy multiple myeloma with IgG or IgA MGUS clones were subsequently identified from the three trial

285 combinant ZIKV generated from the infectious clone, which contains the VNDT motif, is highly pathogen

286 res contain a substantial fraction of public clones, which may be defined as Ab or TCR clonal sequenc

287 (1st Hit) at the stem-cell level generates a clone with replicative advantage.

288 c regulators, recovering bi-allelic targeted clones with a high efficiency of 60% and used Flp recomb

289 Briefly, stable astrocytes clones with an integrated fluorescent HIV reporter and C

290 Screening for desired clones with CRISPR-mediated genomic edits in a large num

291 Myoblast clones with CRISPR/Cas9-mediated knockout of C3G failed

292 y-used HBV-inducible cell line, contain cell clones with diverse permissiveness to HBV replication.

293 roto-oncogenes and by increased abundance of clones with integrations near MECOM and LMO2 These clone

294 to the convergent evolution of fast-growing clones with mitotic checkpoint defects.

295 ing reveals molecular similarities of mutant clones with native PanINs, and identifies potential PanI

296 In summary, IL-17-producing alphabeta T cell clones with psoriasis-specific antigen receptors exist i

297 rm survivors of pancreatic cancer, including clones with specificity to both high-quality neoantigens

298 nome-wide methylation profiles for different clones within almond genotypes were developed to examine

299 When examining clones within the same patient, 30 (68%) of 44 individua

300 In contrast, permanence of naive T cell clones would be determined by their affinity for cognate