ライフサイエンスコーパス: clonotypic

1 Maternal transfer of TCR clonotypic Ab protected young NOD mice against the adopt

2 TCR-IgG1-immunized AKR/J mothers making D10 clonotypic Ab, the effect was immunologically specific.

3 ssion difference likely lowers levels of the clonotypic AI4 TCR in NOD, but not B6.H2g7 thymocytes, b

4 an oligomeric protein complex consisting of clonotypic alpha beta polypeptides associated with invar

5 receptor (TCR)-alpha gene locus to generate clonotypic alpha/beta TCR, after which a few cells expre

6 Here we used the clonotypic alphabeta chains of the T cell receptor (TCR)

7 tocompatibility complex (MHC) molecule via a clonotypic alphabeta heterodimeric structure (Ti) non-co

8 t T lymphocytes consists of disulfide-linked clonotypic alphabeta heterodimers noncovalently associat

9 mechanism through which the interaction of a clonotypic alphabeta T-cell receptor (TCR) with a peptid

10 plasma membrane with a clone-specific (i.e. clonotypic) alphabeta heterodimer that binds its cognate

11 Clonotypic analyses of the reconstituted TRBV4-1(+) TCR

12 More generally, clonotypic analysis and comparison of the variable porti

13 udy provides the first proof-of-concept that clonotypic analysis may be used as a tool to monitor the

14 oire formation, we conducted a comprehensive clonotypic analysis of CD8(+) T cell populations directe

15 Clonotypic analysis of responses to B51-TI8 revealed a p

16 Clonotypic analysis of TCRs in fresh RCCs showed that EM

17 Clonotypic analysis of the two iNK TCR types with a sing

18 Clonotypic analysis revealed that the HIV-specific CD4(+

19 ed potent anti-glioma therapeutic effects in clonotypic and genetically engineered murine models of g

20 ong the ligands QL9/L(d), SIYR/K(b), and the clonotypic antibody 1B2.

21 recognition and indicated that the pMHC and clonotypic antibody epitopes on the TCR were similar.

22 nal antibody (mAb) 1F7 as a therapeutic anti-clonotypic antibody in HIV-1-infected patients, we used

23 To test the applicability of clonotypic assays, we developed rational molecular metho

24 al was to assess the behavior of circulating clonotypic B cells clinically.

25 wever, has never been corroborated, and such clonotypic B cells have never been documented in HL pati

26 ugh the clinical significance of circulating clonotypic B cells in HL remains unclear, these data sug

27 ma plasma cells prompted the notion that the clonotypic B cells that exist in the blood and bone marr

28 Rituximab-ABVD and clonotypic B cells warrant additional study in classical

29 Persistence of detectable circulating clonotypic B cells was associated with a greater relapse

30 s consistently show an absence of pre-switch clonotypic B cells, while M-PCs circulate in the periphe

31 Our results suggest that circulating clonotypic B-cell populations represent multiple myeloma

32 is dependent on the surface expression of a clonotypic B-cell receptor complex (BCR) consisting of m

33 ne whether the immunogenetic features of the clonotypic B-cell receptors (BcR) may identify different

34 reaction-based studies suggested pre-switch "clonotypic" B cells sharing the immunoglobulin (Ig) rear

35 Immunogenetic studies of the clonotypic BCR Ig of CLL subset #4 suggested a resemblan

36 cytic leukemia (CLL) subgroups with distinct clonotypic BCRs present discrete patterns of TLR express

37 B lymphocyte involvement, the proportion of clonotypic cells among the CD19-expressing cells from my

38 ple myeloma are undefined, and contaminating clonotypic cells could contribute to disease recurrence.

39 was 0.0009% (range, 0%-0.1%) or 0.5 x 10(4) clonotypic cells per kilogram (range, 0-41.2 x 10(4)/kg)

40 h allows specific recognition of unassembled clonotypic chains by the ER chaperone machinery and, the

41 The clonotypic characteristics of CD8 T-cell populations eli

42 Here, the clonotypic composition of CMV-specific CTL was determine

43 itude, specificity, phenotype, function, and clonotypic composition of CMV-specific T-cell responses

44 Previous studies assessed the clonotypic composition of CTL specific for individual im

45 ns was linked to a substantial switch in the clonotypic composition of epitope-specific CD8+ T cells,

46 Ag presentation patterns in determining the clonotypic composition of vaccine-induced T cell respons

47 ype that was not characterized by a distinct clonotypic composition.

48 clonal, highly skewed, and exhibited diverse clonotypic configurations; TCRB CDR3 sequence analysis i

49 Estimated clonotypic contamination per SCC was 0.0009% (range, 0%-

50 Melphalan-mobilized SCCs contain minimal clonotypic contamination.

51 itiated after CD94 ligation and suggest that clonotypic differences in signaling generate fundamental

52 consists of at least seven polypeptides: the clonotypic, disulfide-linked alpha/beta heterodimer that

53 eficial to the host, yet the determinants of clonotypic diversity are poorly defined.

54 ar T cell response size on immunization, the clonotypic diversity of the CD4 T cell response correlat

55 s is the primary parameter that dictates the clonotypic diversity of the responding CD4 T cell compar

56 Despite the enormous clonotypic diversity that resides within the naive T-cel

57 We measured CD8 T cell clonotypic diversity to three epitopes recognized in C57

58 decline in the fraction of singletons and in clonotypic diversity.

59 homeostatic competition, result in narrowed clonotypic diversity.

60 pensation mitigates this process to maintain clonotypic diversity.

61 normal subjects is characterized by striking clonotypic dominance and the potential for epitope focus

62 n 15 pediatric patients by screening for the clonotypic E2A-PBX1 translocation in neonatal blood spot

63 Clonotypic elimination of activated T cells through Fas-

64 tored by human adenovirus serotype 5 induced clonotypic expansion irrespective of avidity, eliciting

65 Nonselective clonotypic expansion was caused by relatively weak adeno

66 re is progressively diversified by staggered clonotypic expansion, according to functional avidity, w

67 CD8(+) T-cell response magnitude, reflecting clonotypic expansions and contractions related to altera

68 ific T cell responses are often comprised of clonotypic expansions with distinct functional propertie

69 of CD8(+) TILs and TCR beta chain (TCRbeta) clonotypic frequency in melanoma tumors to identify pati

70 n has been endorsed by the identification of clonotypic gene fusion sequences in archived neonatal bl

71 Clonotypic genomic AML1-ETO sequences were detected in t

72 l-time PCR assay to measure the frequency of clonotypic HCV-specific CD8(+) T cells in peripheral blo

73 uctural biology of the Fab-like TCRalphabeta clonotypic heterodimer and its unique features in conjun

74 two functional domains: the antigen-binding clonotypic heterodimer and the signal-transducing invari

75 In most vertebrates, there are two different clonotypic heterodimers (TCRalphabeta and TCRgammadelta)

76 T cells differ not only in their respective clonotypic heterodimers but also in the subunit composit

77 (TCR) and pre-TCR complexes are composed of clonotypic heterodimers in association with dimers of si

78 this work we take advantage of the profound clonotypic hierarchies of the large human CD4(+) T cell

79 This analysis revealed a stable clonotypic hierarchy in which 1-3 dominant clonotypes ar

80 morphologic remission and that expansion of clonotypic HSCs precedes clinical relapse.

81 in chronically infected primates as an anti-clonotypic immunogen to boost antibodies that neutralize

82 ith lack of somatic hypermutation within the clonotypic immunoglobulin heavy variable (IGHV) genes.

83 Clonotypic immunoglobulin rearrangements were detected i

84 udy, we manipulated TCRs in vivo to generate clonotypic iNKT cells using TCR retrogenic chimeras.

85 In 4 cases, the clonotypic leukemic Vdelta2-Ddelta3 rearrangement was de

86 of fresh (unexpanded) memory T cells at the clonotypic level.

87 o decreased proliferative capacity of BDC2.5 clonotypic-like cells.

88 ne a T cell memory repertoire as the pool of clonotypic lineages participating in a recall response t

89 expression within the lung, and an influx of clonotypic lymphocytes.

90 CS analysis or immunohistochemistry with the clonotypic mAb KJ1-26.

91 In classical Hodgkin lymphoma, circulating clonotypic malignant cells express CD20, which potential

92 S mu/c-myc junctions are thus useful clonotypic markers for monitoring the conversion of Igh

93 Clonotypic markers revealed different plaque and adventi

94 n the analysis of recombination sequences as clonotypic markers, migration of c-myc recombination-pos

95 f these cells through the use of congenic or clonotypic markers.

96 says were capable of identifying circulating clonotypic memory B-cell populations within the peripher

97 lloantigen Ld and can be identified with the clonotypic monoclonal antibody 1B2.

98 Using clonotypic oligonucleotide probes to quantify the size o

99 , the maintenance of the initially recruited clonotypic pattern of HIV-1-specific CD8+ T cells was as

100 at nodular PR in CLL represents MRD and that clonotypic PCR detects MRD in CLL more frequently than f

101 vast majority of patients remain positive by clonotypic PCR.

102 tment biopsies were subjected to semi-nested clonotypic PCR.

103 on of the HIV-specific T cell repertoire and clonotypic phenotype and function.

104 e a minimal deleted region of chromosome 13, clonotypic plasma cells from 50 consecutive patients wit

105 T fusion was present in more than 80% of the clonotypic plasma cells in these novel cases, there was

106 ase (MRD) in a highly specific and sensitive clonotypic polymerase chain reaction (cPCR).

107 cluded flow cytometry and a highly sensitive clonotypic polymerase chain reaction (PCR).

108 We determined molecular remission status by clonotypic polymerase chain reaction (PCR).

109 nt study, IL-2 expression and signaling in a clonotypic population of antiviral CD4+ T cells was anal

110 ositional scanning format with two different clonotypic populations of CD4+ T cells to identify pepti

111 tification of peptide ligands that stimulate clonotypic populations of T cells.

112 Nested CDR-III clonotypic primers were used in a semi-nested cPCR with

113 ones in different cell populations using TCR clonotypic probing.

114 pheral blood after transplantation by use of clonotypic quantitative real-time PCR.

115 ells was done in 13 myeloma patients using a clonotypic, quantitative allele-specific oligonucleotide

116 Using clonotypic real-time RT-PCR, we have detected low freque

117 IgA repertoires of myeloma patients for the clonotypic rearrangement by next-generation sequencing.

118 ost-switch IgG/IgA repertoires, however, the clonotypic rearrangement was detected at high frequency

119 cells were then analyzed for the presence of clonotypic rearrangements of the T-cell receptor (TCR) V

120 oma patients and that this population shares clonotypic rearrangements with the malignant plasma cell

121 creas, actively turning over, expressing the clonotypic receptor, and containing functional regulator

122 CD8+ T cells respond to Ags when their clonotypic receptor, the TCR, recognizes nonself peptide

123 For the clonotypic receptors of B and T cells and for Fc recepto

124 most, if not all, memory T cells expressing clonotypic receptors that bind a tetrameric complex of i

125 nd to foreign antigenic peptides using their clonotypic receptors.

126 V9, both T cell populations displayed biased clonotypic repertoires and reacted similarly against HLA

127 es 1 (IGHV1) and 5 (IGHV5) was higher in IgE clonotypic repertoires compared with other antibody clas

128 ell populations with architecturally diverse clonotypic repertoires that displayed potent lytic activ

129 Conversely, diverse clonotypic repertoires without discernible motifs were n

130 sponse to rechallenge, the magnitude of each clonotypic response was 10-fold higher in the liver than

131 T cell clones identified by their clonotypic sequence as expanded in the valve were also f

132 We identified the clonotypic sequence of T-LGL clones in 60 patients, incl

133 ion status, functionality, distribution, and clonotypic structure of MAIT cell populations in the per

134 gamma delta T cells are distinguished by the clonotypic subunits contained within their TCRs.

135 The clonotypic surface Ig receptor expressed by malignant B

136 conditions of Ab excess, but also to prevent clonotypic T cell activation by inhibiting the ability o

137 rmed double immunohistochemical staining and clonotypic T cell receptor (TCR) beta-chain sequencing i

138 Clonotypic T cell receptor (TCR) genes undergo ordered r

139 D8+ T cells to the self-specificity of their clonotypic T cell receptor (TCR).

140 If a clonotypic T cell receptor expressed on a circulating T

141 ell maturation requires the rearrangement of clonotypic T cell receptors (TCR) capable of interacting

142 The remaining clonotypic T cells are unresponsive to antigenic stimula

143 of Treg, the lack of peripheral expansion of clonotypic T cells is due to the absence of its high-aff

144 In contrast, Vbeta5(high)Valpha2(high) clonotypic T cells were not expanded, displayed the naiv

145 s fail to prevent autoimmunity by LAG-3(-/-) clonotypic T cells, implicating an important role for LA

146 13 inhibited proliferation of islet-reactive clonotypic T cells.

147 olecules (pMHCI or pMHCII, respectively) via clonotypic T-cell receptors (TCRs) remains debated.

148 we performed a comprehensive analysis of the clonotypic T-cell response using complementary murine mo

149 r monitoring corresponding clones, including clonotypic Taqman polymerase chain reaction (PCR) and cl

150 result in high vs low expression of the AI4 clonotypic TCR alpha-chain on developing thymocytes in B

151 gative biopsy samples showed no evidence for clonotypic TCR amplification.

152 Using clonotypic TCR beta-chain length and sequence analysis w

153 Addition of antibody to CD3 or the clonotypic TCR caused rapid CTCL cell apoptosis followed

154 signal CD4 commitment even in the absence of clonotypic TCR chains.

155 R structure, and is blocked by a recombinant clonotypic TCR comprised of TRAV17 and TRBV4-1, proving

156 unity provides a unique example in which the clonotypic TCR conveys the Th2 disease phenotype.

157 s development; notably, Tregs expressing the clonotypic TCR did not.

158 clusion, thymocytes with lower levels of the clonotypic TCR evaded deletion in TS1 x HA12 and TS1 x H

159 roducing T cells and to specifically measure clonotypic TCR frequencies in the T cell pool.

160 r a mimetic epitope recognized by the BDC2.5 clonotypic TCR in NOD mice.

161 ave a minimal loss of T cells expressing the clonotypic TCR in the thymus and spleen.

162 ression of effector CD4(+) T cells using the clonotypic TCR in vivo, but failed to mediate bystander

163 hritis, in which CD4(+) T cells expressing a clonotypic TCR induce disease by an IL-17-dependent mech

164 pheral self-antigen; should the autoreactive clonotypic TCR induce T conv to T reg cell conversion up

165 viously developed a useful method to isolate clonotypic TCR sequences from Ag-specific IFN-gamma-prod

166 (TCR) transgenic (Tg) mice that expressed a clonotypic TCR specific for a class II major histocompat

167 TCR) transgenic (Tg) recipients expressing a clonotypic TCR specific for an allogeneic major histocom

168 anchored RT-PCR, and real-time quantitative clonotypic TCR tracking.

169 HA, thymocytes expressing high levels of the clonotypic TCR were deleted in both HA-transgenic lineag

170 at the majority of the T-cells expressed the clonotypic TCR, and the phenotype and function of the ce

171 n early thymocytes, before the initiation of clonotypic TCR-alpha and TCR-beta gene rearrangement but

172 and are increased in cells that express the clonotypic TCR.

173 ticulin as a molecular chaperone for nascent clonotypic TCRalpha and -beta proteins and demonstrate t

174 that, unlike calnexin, which associated with clonotypic TCRalpha and -beta proteins and invariant CD3

175 s, calreticulin associated specifically with clonotypic TCRalpha and -beta proteins.

176 on is mediated through direct interaction of clonotypic TCRs with complexes formed between Ag-present

177 solation of specific T-cell clones and their clonotypic TCRs.

178 utiny of the DNA from an archived slide with clonotypic TEL-AML1 primers showed that the presumptive

179 aemia at age 4 years, who shared a single or clonotypic TEL-AML1 sequence that suggested prenatal ori

180 None of 12 cases showed pre-switch clonotypic transcripts.

181 These clonotypic Tregs exerted Ag-specific suppression of effe

182 mechanism of T cell activation showed that a clonotypic Valpha2/Vbeta21 TCR transmitted activating si