ライフサイエンスコーパス: cloverleaf

1 uces its secondary structure to a 'truncated cloverleaf'.

2 istent with a model in which the 5'-terminal cloverleaf and 3' NTRs of poliovirus RNA interact via te

3 insight into key functional elements in the cloverleaf and IRES, thereby establishing a base of stru

4 des 102/103, mapping to a region between the cloverleaf and the internal ribosome entry site (IRES) i

5 rved nucleotide (A(103)) located between the cloverleaf and the IRES which is important for replicati

6 located in any of the four stems of the tRNA cloverleaf and usually create a G.C base pair.

7 ich nucleotides and/or structures within the cloverleaf are essential for 2C binding.

8 support the hypothesis that the modern tRNA cloverleaf arose from a single hairpin duplication prior

9 efoxitin-induced nitrocefin test, penicillin cloverleaf assay, and penicillin disk zone edge test.

10 contains two highly structured regions, the cloverleaf (CL) and the internal ribosomal entry site (I

11 macrostructural level, AFMs are grouped into cloverleaf clusters, an organizational structure also se

12 Mutations within the cloverleaf destabilized viral RNA in these reactions.

13 '-nontranslated regions were confined to the cloverleaf domain and localized within the region of the

14 These departures from the secondary cloverleaf form call into question the universality of t

15 single particles collected at six different cloverleaf freeway on-ramps in Southern Michigan, using

16 The necessary region of the cloverleaf has previously been narrowed to a highly cons

17 poliovirus replicons with the gene order [PV]cloverleaf-[HCV]IRES-Deltacore-R-Luc-[PV]IRES-F-Luc-P2,3

18 sequence and the poliovirus (PV) 5'-terminal cloverleaf in a PV/HCV chimeric virus (containing the HC

19 To examine the role of the cloverleaf in poliovirus replication, we determined how

20 The 5' cloverleaf in poliovirus RNA has a direct role in regula

21 we investigated the role of stem a in the 5' cloverleaf in regulating the stability and replication o

22 terminal stem-loop structure (stem-loop I or cloverleaf) in concert with viral protein 3CD is require

23 Therefore, the 5' cloverleaf is a multifunctional cis-acting replication e

24 conserved sequence, UGUUUU, in stem a of the cloverleaf is altered.

25 Strikingly, it also requires a cloverleaf-like RNA structure located at the other end o

26 sed magnetic vortex states, but the observed cloverleaf-like stray fields indicate the presence of we

27 rotein was unable to efficiently bind to the cloverleaf-like structure (CL) at the 5' end of PV1 RNA,

28 entry site, thereby deleting the 5'-terminal cloverleaf-like structure, or insertion of three nucleot

29 cornaviridae family, the 5'UTR consists of a cloverleaf-like terminus preceding the internal ribosoma

30 The resulting structure revealed a compact cloverleaf morphology stabilized by a long-range tertiar

31 in poliovirus replication, we determined how cloverleaf mutations affected the stability, translation

32 s were obtained from echo planar imaging and cloverleaf navigator sequences every 3 s and 20 ms, resp

33 These results indicate that the 5' cloverleaf normally protects uncapped poliovirus RNA fro

34 that interaction of 2C with the 3'-terminal cloverleaf of negative-strand RNA is facilitated when th

35 The 5'-terminal cloverleaf of poliovirus RNA was required in cis to form

36 Binding of 2C to the 3'-terminal cloverleaf of the negative-strand RNA is greatly affecte

37 mic 5' terminus which partially degraded the cloverleaf (or domain I), an RNA structure required for

38 e readily extends up to the modern tRNA-like cloverleaf passing through an intermediate hairpin havin

39 scopy, we observed an intriguing conductive 'cloverleaf' pattern of six domains emerging from one poi

40 of HCN emission from the infrared-luminous 'Cloverleaf' quasar (at a redshift zeta = 2.5579).

41 ajor determinant for interaction between the cloverleaf RNA and viral 3C protease, which is an essent

42 The viral protein 3CD binds to the cloverleaf RNA but does not interact directly with stem-

43 tease 3C and the viral polymerase 3D) to the cloverleaf RNA dramatically increases the affinity of PC

44 Kinetic analyses indicated that the PCBP-5' cloverleaf RNA interaction was necessary to protect PV m

45 C24A mutation that inhibits PCBP-5'-terminal cloverleaf RNA interactions inhibited the formation and

46 Stem-loop D from the cloverleaf RNA is a highly conserved domain within the 5

47 A 5'-terminal cloverleaf RNA structure interacts with poly(rC) binding

48 An RNP complex formed around the 5' cloverleaf RNA structure interacts with the poly(A) bind

49 g tetraloop that occurs naturally within the cloverleaf RNA structure of the 5'-UTR of coxsackievirus

50 reactions, using poliovirus negative-strand cloverleaf RNA, led to a decrease in RNA synthesis.

51 y ternary ribonucleoprotein complex with the cloverleaf RNA, resembling the full-length PCBP protein.

52 A cloverleaf secondary structure and the concomitant "L"-s

53 here coordinating ribozymes, HS01, assumes a cloverleaf secondary structure closely resembling E18, y

54 ed SL-1 to SL-4), which can be arranged in a cloverleaf secondary structure.

55 All 22 tRNA genes had typical cloverleaf secondary structures, except for trnS1 (AGN)

56 ten deviate substantially from the canonical cloverleaf (secondary) or 'L'-shaped (tertiary) structur

57 ose with the potential to form conventional "cloverleaf" secondary structures, (ii) those with TPsiC

58 omparison between nine homologous 'truncated cloverleaf' secondary structures and on analogies with t

59 Significant departures from the canonical (cloverleaf) secondary structure of transfer (t)RNAs can

60 ging by rapid beam oscillation method with a cloverleaf-shaped trajectory in conjunction with the pai

61 tley-Bixler syndromes, and Kleeblaatschadel (cloverleaf skull) deformity.

62 teract with both the 5'-element known as the cloverleaf structure and the large stem-loop IV RNA of t

63 A cloverleaf structure at the 5' terminus of poliovirus RN

64 ted to tRNA genes, although the typical tRNA cloverleaf structure is not apparent for most SINE conse

65 Many studies have suggested that the modern cloverleaf structure of tRNA may have arisen through dup

66 ionary stage preceding the appearance of the cloverleaf structure of tRNA.

67 generate a standard representation (like the cloverleaf structure of tRNAs) or any layout desired by

68 on of single-stranded regions exposed on the cloverleaf structure offered a valid explanation for the

69 f Mg(2+), the mutated tRNA does not form the cloverleaf structure typical of tRNAs.

70 nce this region is believed to form a stable cloverleaf structure, a number of mutations were constru

71 virus 5' untranslated region (5'UTR), the 5' cloverleaf structure, and the stem-loop IV of the intern

72 ting three-stemmed structure to form a proto-cloverleaf structure.

73 ith six-times-higher affinity than to the 5' cloverleaf structure.

74 sequential melting of the four stems of the cloverleaf structure.

75 hese 14 tRNA genes are a mixture of standard cloverleaf structures and nonstandard structures contain

76 s, whereas the penicillin disk zone edge and cloverleaf tests showed sensitivities of 100% but specif

77 rms among the more than one dozen canonical (cloverleaf) tRNAs that have yielded to crystallographic

78 dine or a TPsiC arm) than for the canonical (cloverleaf) tRNAs.

79 Below the melting temperature of the cloverleaf, unmodified yeast tRNAPhe exists in a Mg2+-de

80 ion of the picornaviral genome begins with a cloverleaf which is required for viral replication, due

81 or dihydrouridine (D) loops of the canonical cloverleaf, which are known to confer structural rigidit