ライフサイエンスコーパス: co-transfect

1 0 and TFAP2A interacted only when CITED2 was co-transfected.

2 , activity increased when TLR2 and CD14 were co-transfected.

3 he kinase activity was enhanced when Nck was co-transfected.

4 ld-type (WT), but not AI-forms of FAM20A was co-transfected.

5 tionation of A20 was dramatically altered by co-transfected 14-3-3, providing the first experimental

6 of the hLHR with the nonvisual arrestins in co-transfected 293 cells.

7 nergy to transactivate the bcl-x promoter in co-transfected 293 cells.

8 We observed that in co-transfected 293T cells, LRIG1 forms a complex with ea

9 l death conferred by hsp27 overexpression in co-transfected 293T cells.

10 a more migratory and invasive phenotype, we co-transfected a vimentin-positive human melanoma cell l

11 FOS proto-oncogene promoter was achieved by co-transfecting a modified human FOS promoter-driven pol

12 In this study, we co-transfected AAV2 and AAV8 helper plasmids at differen

13 we produced the triploid vector AAV2/8/9 by co-transfecting AAV2, AAV8 and AAV9 helper plasmids at a

14 Similarly, co-transfected Ala --> Thr-1940 human NMHC-IIA was phosp

15 restores B-Myb transactivating activity when co-transfected along with CDK9, suggesting that the down

16 ffected receptor function dependent upon the co-transfected alpha subunit.

17 ion, and it blocked H11-induced apoptosis in co-transfected and Aza-C-treated cells, indicating that

18 ntact cells was further demonstrated when we co-transfected and co-immunoprecipitated the wild type,

19 d only in extracts from cells that have been co-transfected and not in extracts combined from cells e

20 p130(Cas), inhibited the Ral GEF activity of co-transfected AND-34.

21 Immunolocalization of co-transfected AR and FLAG-beta-catenin demonstrated tha

22 nctional tests were performed by transiently co-transfecting Bax in the context of different Bcl-xL m

23 However, co-transfected bHLH factors regulated activity of the 38

24 ween thrombospondin-4 and alpha2delta-1 when co-transfected, but there was no co-immunoprecipitation

25 with one of the major NF-kappaB proteins, we co-transfected C/EBP-alpha with NF-kappaB p50, p65, p50

26 d show that these effects were enhanced by a co-transfected C/EBPdelta expression plasmid.

27 ha-actin promoter, which lacks GRE sites, in co-transfected C2C12 cells.

28 Co-transfecting Ca(V)beta(2a) with alpha(1C).beta(2b) ha

29 ylation, neither inhibiting this activity by co-transfecting Cdk2DN nor augmenting it with cyclin A r

30 Co-transfected cells had larger Ba2+-sensitive inwardly

31 In a small number of co-transfected cells marked time dependence to the activ

32 These co-transfected cells release an abundance of truncated a

33 expression level on cell membranes of HA/NA co-transfected cells was observed and intracellular traf

34 haves in a dominant-negative manner, we next co-transfected cells with Cav-1 (P132L) and WT Cav-1, an

35 Treatment of co-transfected cells with phosphatidylinositol-specific

36 nover show enhanced association with axin in co-transfected cells, and excess CTD polypeptide can com

37 -bound organellar fraction of Bid plus Bcl-2-co-transfected cells, but not from cells transfected wit

38 the Sig1R and Nav1.5 can be co-isolated from co-transfected cells, consistent with an intimate associ

39 In co-transfected cells, Dll1 is constitutively cleaved by

40 In co-transfected cells, EPRAP, which contains multiple ank

41 rin consensus sequence, was cleaved in furin co-transfected cells, whereas MMP-1, which lacks an RXXR

42 ociation of the rFSHR with the arrestin-3 in co-transfected cells.

43 in nuclear structures in a subpopulation of co-transfected cells.

44 t RetGC1 and co-localize with the cyclase in co-transfected cells.

45 and BK channel coupling were investigated in co-transfected cells.

46 d interacted weakly with the Pax9 protein in co-transfected cells.

47 We co-transfected combinations of chimeric receptors into B

48 bl or a catalytically activated Lck-Y505F in co-transfected COS cells, and by endogenous kinases in t

49 In co-transfected COS cells, Lck and Fyn caused phosphoryla

50 with wild type CD40L on the cell surface of co-transfected COS cells.

51 d with alpha(o) sequence was investigated in co-transfected COS-7 cells [studied biochemical response

52 imally stimulate transcriptional activity in co-transfected COS-7 cells.

53 ith p107, and B-Myb enhanced the activity of co-transfected cyclin E kinase activity, implying that B

54 ication was first emphasized by showing that co-transfected dominant-negative Cdk2 (Cdk2DN) substanti

55 Co-transfected EKLF was synergistic with either Sp1 or S

56 ession of IRS-1 and -2 on translocation of a co-transfected epitope-tagged GLUT4 (GLUT4-HA).

57 cts on insulin-stimulated translocation of a co-transfected epitope-tagged GLUT4 were studied.

58 ression enhanced FN-stimulated activation of co-transfected ERK2.

59 tively nuclear FoxO1 mutant transactivated a co-transfected ET-1 promoter luciferase reporter.

60 , two plasmids encoding a pair of TALENs are co-transfected, followed by limited dilution to isolate

61 ect on cell proliferation or expression of a co-transfected gene.

62 a channel composed of these two subunits, we co-transfected GIRK2 and GIRK3 in CHO-K1 cells and assay

63 erior cervical sympathetic (SCG) neurones by co-transfecting green fluorescent protein (GFP)-, GIRK1-

64 tial alterations to the gene targeted by the co-transfected guide RNA.

65 The second strategy involved co-transfecting H4IIE cells with a pPL32 mutant, in whic

66 IP12 interaction in acinar cell lines and in co-transfected HEK-293T cells.

67 increased in cdk5/p35 and hNF-H tail domain co-transfected HEK293 cells grown on laminin.

68 Mrp2 co-precipitated with NHERF-1 in co-transfected HEK293 cells, an interaction that require

69 In co-transfected HEK293 cells, RNF34 reduces the expressio

70 ependent, staurosporine-induced apoptosis in co-transfected HEK293 cells.

71 ess their role in GCAP1 binding to RetGC1 in co-transfected HEK293 cells.

72 This result was confirmed by co-transfecting HEK293 cells with the sodium-phosphate c

73 T5 specifically interacts with GATA4 in both co-transfected HEK293T cells and neonatal rat cardiomyoc

74 CaMKK and 14-3-3 co-immunoprecipitated from co-transfected heterologous cells as well as from rat br

75 When co-transfected, HIP-55 increased HPK1's kinase activity

76 alysis of nuclear extracts from RelA and AhR co-transfected Hs578T cells.

77 ent associated with a full-length protein in co-transfected human embryonic kidney 293 cells.

78 g subunit of the IL-6 receptor, gp130, or by co-transfected IL-3 receptor.

79 tor receptor I (TNFRI) expression vector was co-transfected in inhibition studies.

80 they were only able to evoke a response when co-transfected, indicating that both are required for fu

81 nked TD-TFOs were pre-incubated in vitro and co-transfected into cells led to conversion frequencies

82 II-15, or I-10 segments were constructed and co-transfected into COS-7 cells with either the V(+)C(+)

83 When sets of mutated cDNAs were co-transfected into FPGS-null cells to mimic the genotyp

84 of a ligand-inducible chimaera HER-1/2, was co-transfected into HEK-293 fibroblasts with a constitut

85 hen a rat HNF-4alpha expression construct is co-transfected into HeLa cells, factor XI promoter activ

86 d yellow fluorescent protein-tagged tau were co-transfected into human embryonic kidney cells.

87 Human ICAM-1 (CD54) and E-selectin were co-transfected into L cells.

88 c and Caspy co-localized to the "speck" when co-transfected into mammalian cells.

89 and STAT3 small interfering RNA (siRNA) were co-transfected into MM1 cells.

90 bsence of androgen when androgen receptor is co-transfected into monkey kidney CV1 cells or human pro

91 ability when Galpha12 and c-raf-1 cDNAs were co-transfected into NIH3T3 cells.

92 yltransferase gene (sgk-CAT) and transiently co-transfected into nontumorigenic NMuMG or transformed

93 either a dominant negative form of NF-Y was co-transfected into the cells or when the GPAT promoter

94 nstructed and then transfected separately or co-transfected into the cells.

95 der the control of a TS-response element was co-transfected into these same cells, and luciferase act

96 creases Ca2+ entry induced by stimulation of co-transfected M5 muscarinic acetylcholine receptor with

97 in-supernatant crossed parental mAbs, or c) co-transfected mAbs.

98 re observed with stable transfections, using co-transfected marker plasmids for selection.

99 and this proliferation defect was rescued by co-transfected mature micro-RNA.

100 detectable effect on the activity of known, co-transfected mitogen-activated protein kinases, sugges

101 rf4 RNAi and a MEF2 reporter is inhibited by co-transfected MRF4 and activated by Mrf4 RNAi.

102 3 protein was not inhibited by endogenous or co-transfected mutant p53 -proteins.

103 hion in both muscle-derived L6 and REST/NRSF co-transfected neuronal PC12 cells.

104 ced with RANKL treatment and unresponsive to co-transfected NFATc1.

105 moter was unresponsive to RANKL treatment or co-transfected NFATc1.

106 tes drastically reduced transactivation by a co-transfected NFI-A expression vector in HeLa cells.

107 r the human or mouse Tbx22 gene promoters in co-transfected NIH3T3 cells.

108 When the helper plasmids are co-transfected onto human 293 cells with a vector plasmi

109 placed by Arg, increased the activity of the co-transfected p21Waf1 promoter, which demonstrates that

110 oxic MCF7 cells with HIF-1alpha stimulates a co-transfected p53-dependent reporter plasmid and increa

111 ect interaction of native TFIID (TBP) with a co-transfected p65 fusion protein.

112 Immunofluorescence microscopy showed that co-transfected PLD2 and Grb2 re-localize to Golgi-like s

113 sed retinoic acid-activated transcription of co-transfected RARbeta2, RARbeta4, and RARalpha.

114 lpha in GHFT1-5 cells strongly activated the co-transfected rGH promoter through the GHF3 binding sit

115 ol phosphate (IP) production mediated by the co-transfected rPAFR cDNA.

116 Functional studies showed that co-transfected RXRalpha, but not T3 receptor beta1 (TRbe

117 n cell lines (HEK293, JEG and TE671) using a co-transfected SEAP-CMV plasmid as a control.

118 n 80% and 95% reduction in the expression of co-transfected selenoprotein genes encoding type I and I

119 We co-transfected siRNA with QDs using standard transfectio

120 agen promoter activity, an effect blocked by co-transfected Smad3EPSM, but not Smad3A.

121 onstruct in U9 cells even in the presence of co-transfected smad4, or alter p21cip1 mRNA levels.

122 Tbx2, and Tbx5 were sufficient to stimulate co-transfected SRF reporter activity, however this activ

123 urface expression of each transporter in the co-transfected system were similar to those of each tran

124 cultured cells detects a complex containing co-transfected tagged S6 ATPase (or endogenous S6) and e

125 interfered with the direct interaction of a co-transfected TBP fusion protein with the native p65 su

126 carrying a gene of interest, one can either co-transfect the alphoid DNA and the gene of interest, o

127 To examine this possibility, we stably co-transfected the MMTV promoter and the GR into T47D ce

128 Both activities were rescued by co-transfecting the cells to express constitutively acti

129 ene was unresponsive to T3 in the absence of co-transfected thyroid hormone receptor.

130 ha1c expression, but when Sox6 and Prtb were co-transfected to the cells, L-type Ca2+ alpha1c remaine

131 ate the breakdown of this class of mRNAs, we co-transfected TTP and its related CCCH proteins into 29

132 trol genes on either the same plasmid or one co-transfected were not affected by the treatment.

133 gp2/mdr1b promoter was strongly activated by co-transfected wild type Sp1 but not mutant Sp1 and muta

134 Co-transfected wild-type human MAT2A mRNA rescued defect

135 of the mutations inhibited signaling through co-transfected wild-type receptors.

136 In U9 cells co-transfected wild-type smad4, but not mutant smad4, me

137 However, when co-transfected with a cDNA encoding truncated TPO, TGD e

138 CWSV1, CWSV2, and CWSV14 cells were co-transfected with a DNA construct containing a dimer o

139 Lec2-NCAM was co-transfected with a human fetal brain cDNA library, a

140 miRNAs, miR-377 and miR-217, were cloned and co-transfected with a luciferase vector containing the h

141 domains were modified in alphaCGbeta, it was co-transfected with a monomeric alpha or CGbeta gene.

142 cells expressing the full-length mVps24p are co-transfected with a mutated form of mVps4p (which is d

143 moter activity of this construct in U2 cells co-transfected with a PG receptor isoform A (PR(A)) expr

144 ion approximately 10-fold in human 293 cells co-transfected with a plasmid containing the rhodopsin p

145 n cells containing such integrated units are co-transfected with a plasmid encoding yeast FLP recombi

146 ease (45-fold) in promoter activity in cells co-transfected with a plasmid expressing the transcripti

147 iver-derived HepG2 cell line was transiently co-transfected with a series of G6Pase-chloramphenicol a

148 AP-1 and PEA3/Ets binding elements and, when co-transfected with a src expression plasmid into HepG2

149 Tat-bearing cells or into endothelial cells co-transfected with a Tat expression vector resulted in

150 stimulate luciferase activity, whereas cells co-transfected with a transcriptionally active glucocort

151 ved in PC-3 cells only when these cells were co-transfected with a wild-type androgen receptor expres

152 n HeLa cells expressing HA-tagged PFK-2 were co-transfected with active PKB or stimulated with IGF-1,

153 een in vanadate-treated COS-7 cells that are co-transfected with alpha-actinin and wild type FAK.

154 a2L subunit sequence, and HEK293T cells were co-transfected with alpha1 and beta3 subunits or alpha1

155 n recombinant gamma2 and delta subunits when co-transfected with alpha1 and/or beta2 subunits in HEK2

156 COS-7 cells were co-transfected with alpha2C10 or alpha2C4 without or wit

157 We found that in membranes from cells co-transfected with alpha2delta-1 and thrombospondin-4,

158 HEK 293 cells were stably co-transfected with alphaV and either beta3C (HEKbeta3C)

159 ls transfected only with reporter plasmid or co-transfected with an active mutant of MAPK kinase-6, a

160 In experiments where the A-CREB mutant was co-transfected with an AP-1 reporter construct, transcri

161 gnificantly reduced reporter expression when co-transfected with an APP 3'-UTR reporter construct.

162 rase reporter in phorbol-ester-treated cells co-transfected with an expression vector encoding the c-

163 incubated in sterol-depleted medium or were co-transfected with an expression vector encoding transc

164 st, parallel experiments in HepG2 cell lines co-transfected with an hPXR expression vector did not sh

165 un driven by a human keratin 14 promoter was co-transfected with AP-1 or NF-kappaB reporters, both AP

166 In TAg-Jurkat T cells transiently co-transfected with apoaequorin and human Edg2 or Edg4 c

167 much less than that of beta2AR even in cells co-transfected with arrestin-2, which increases beta1AR

168 romoter activity were detected in HS68 cells co-transfected with bax-luc and empty vector, N239S, or

169 onal activity even when pituitary cells were co-transfected with Bob1.

170 In COS cells co-transfected with both G alpha 16 and the C5a receptor

171 ith wild-type Amelx, mutant Amelx or Ambn or co-transfected with both wild-type Amelx and Ambn.

172 transformation was also suppressed in cells co-transfected with BPV-1 plus ts and in ts expressing c

173 urin-stimulated decrease of Po,peak in cells co-transfected with C alpha-PKA is blocked by 800 nM del

174 -down of whole cell GluR6 responses in cells co-transfected with C alpha-PKA.

175 pitope-tagged constructs of MAP kinases were co-transfected with CaM-KKc plus CaM-KIVc, the immunopre

176 Hippocampal neurons co-transfected with CB2(SH3-) and gephyrin developed ver

177 Chinese hamster ovary cells were co-transfected with cDNA constructs encoding mutant beta

178 is-retinoic acid from 9-cis-retinol in cells co-transfected with cDNAs encoding ALDH12 and the 9-cis-

179 The cells were co-transfected with cDNAs for the glycosyltransferases r

180 When epitope-tagged GAB1 was co-transfected with constitutively active MEK1 into A293

181 nsactivation was markedly increased in cells co-transfected with constitutively active MKK1.

182 on ROMK1 was completely blocked in the cells co-transfected with dominant negative dynamin (dynaminK4

183 We demonstrate here that pcPNAs, when co-transfected with donor DNA fragments, can promote sin

184 When HEK293T cells were co-transfected with Edg-8 and G protein DNAs, prepared m

185 also observed in Chinese hamster ovary cells co-transfected with EGFR and herstatin.

186 ession vectors for visual cycle proteins and co-transfected with either fatty acyl:CoA ligases (ACSLs

187 The RSV RBE(apt) plasmid co-transfected with either HIV clone, at the same weight

188 d with anti-FLAG IgG1 from lysates of HEK293 co-transfected with either Myc-Hic-5 or Myc-paxillin and

189 primary hippocampal neurons, and Cos7 cells co-transfected with ERalpha and alphaCaMKII.

190 nificant reduction in promoter activity when co-transfected with Ets-2 or exposed to LPS.

191 om COS-1 cells is selectively inhibited when co-transfected with expression vectors encoding the alph

192 Immunofluorescence of cells co-transfected with FAK and Trio revealed significant co

193 ls and was highly phosphorylated when it was co-transfected with FAK.

194 transfected with EGFP-rap1A or EGFP-rap1B or co-transfected with FLAG-tagged rap1GAP.

195 ammaS in f-MLF-stimulated membranes of cells co-transfected with FPR.

196 lpha7 or dupDeltaalpha7, and constructs were co-transfected with full-length alpha7 in Neuro2a cells.

197 Cells co-transfected with full-length and mutant forms of CSF2

198 By contrast, cells co-transfected with FUT1 plus either GCNT1, GCNT2, or GC

199 When cells were first co-transfected with GAL3ST3 and B3GNT7 and then with CHS

200 When HEK293T cells were co-transfected with GAL3ST3 and B3GNT7 expression vector

201 When co-transfected with Galpha(13)Q226L, the C-terminal doma

202 experiments in which reporter constructs are co-transfected with GLI1, suggest a direct interaction b

203 In BHK cells co-transfected with guanylate cyclase and G-kinase expre

204 COS-7 cells co-transfected with Gyc-88E and Gyc-89Da or Gyc-89Db acc

205 lysates of normoxic PC3M cells that had been co-transfected with haemagglutinin-tagged HIF-1alpha and

206 sing a dominant-negative mutant of hTLR2 was co-transfected with hBD2-promoter-LUC into EC, LTA could

207 ylmethionine decarboxylase) from COS-7 cells co-transfected with hemagglutinin-tagged ubiquitin and s

208 Cells co-transfected with hemojuvelin (HJV) and matriptase-2 (

209 and identical to currents expressed by cells co-transfected with independent MinK and KvLQT1 cDNA.

210 In contrast, cells co-transfected with Kir6.2-C-GFP and SUR1 exhibited inte

211 In cells co-transfected with Kir6.2-C-GFP and SUR1, strong membra

212 hereas the opposite was true for NL4-3/CIDHP co-transfected with mu-36p66ShcA (micro-36) dominant neg

213 and GST Ya genes expression in Hep-G2 cells co-transfected with Nrf2 and small Maf proteins.

214 g an IFN-responsive ISG54 reporter construct co-transfected with ORF K9 into HeLa and 293 cells.

215 n epitope-tagged ERK (HA-ERK) or HA-JNK were co-transfected with other vectors by using adenovirus-po

216 rate of p105 is markedly inhibited in cells co-transfected with p50 or other NF-kappaB subunits, inc

217 Whereas, Hep3B co-transfected with p53 and GADD45beta demonstrated sign

218 r transactivation ability was repressed when co-transfected with Pax6.

219 Here, by using 293A cells co-transfected with pcDNA3-hBVR and PKC betaII plasmids,

220 When co-transfected with pcDNA3/SP-C-(1-194), both EGFP/SP-C-

221 Addition of aluminum fluoride to cells co-transfected with PKD and wild type Galpha(13) also in

222 The addition of aluminum fluoride to cells co-transfected with PKD and wild type Galpha(q) induced

223 ffect also was observed when HeLa cells were co-transfected with plasmids encoding wild-type and muta

224 ate endosomes, HEK293 cells were transiently co-transfected with scavenger receptor A (SR-A) and incu

225 the cell membrane was enhanced when ENaC was co-transfected with sgk1 in kidney cells, suggesting tha

226 d was tyrosine phosphorylated in COS-7 cells co-transfected with Shd and c-Abl or Bcr-Abl.

227 1 against serum deprivation was reduced when co-transfected with SKIP.

228 in vitro by SKK3 (also called MKK6) or when co-transfected with SKK3 into COS cells.

229 LC2v and cardiac actin promoters when it was co-transfected with SRF, but it repressed atrial natriur

230 nstitution system using TACE-deficient cells co-transfected with TACE and substrate cDNAs to study TA

231 intracellular calcium unless the cells were co-transfected with the alpha subunit of Gq.

232 Cells co-transfected with the Ca(2+)-sensitive probe aequorin

233 ar level, primary fetal rat osteoblasts were co-transfected with the estrogen receptor (hER, to ensur

234 tRNA suppressor containing CUA anticodon is co-transfected with the GFP gene.

235 r and ribozyme DNAs was tested in HeLa cells co-transfected with the HIV-1 proviral clones, HXBDeltaB

236 ecreted alkaline phosphatase in HEK293 cells co-transfected with the human TLR2 and secreted embryoni

237 CV-1 cells co-transfected with the luciferase gene under peroxisome

238 reased the luciferase activity of Spry1 when co-transfected with the mouse Spry1 3'-UTR reporter cons

239 ctor pAvCvSv-hTopIIalpha was constructed and co-transfected with the pBHG10 packaging vector into 293

240 of Ds was detected only in those cell nuclei co-transfected with the replicating Ds-vector DNA and a

241 approximately 27-fold in mammalian 293 cells co-transfected with the rhodopsin luciferase reporter, s

242 Fq/11 cells were co-transfected with the TRH receptor and a green fluores

243 d, induced NFkappaB activation when TLR2 was co-transfected with TLR1 or TLR6 in 293T cells, but not

244 ivation of Chinese hamster ovary (CHO) cells co-transfected with TLR4 and mutant MD-2.

245 Trunk and Torso-like were active when co-transfected with Torso and when presented to Torso-ex

246 recombinant Mos1 or Mboumar-9 transposase is co-transfected with transposon-containing plasmid DNA, i

247 opressin (AVP)-induced Ca(2+) entry in cells co-transfected with TRPC6 and AVP V1 receptor.

248 scence intensity on the surface of the cells co-transfected with TRPC6-EGFP and V1 receptor, and this

249 ption, GATA-5 and -6 expression vectors were co-transfected with TTF-1 luciferase expression vector.

250 n stimulated HEK293 and HeLa cells that were co-transfected with TurboGFP, suggesting that GFP interf

251 play an increase in hydrolytic activity when co-transfected with wild type ALBP.

252 When cells were transiently co-transfected with wild-type hLHR and a misfolded mutan

253 and acts in a dominant-negative fashion when co-transfected with wild-type RARalpha.

254 by mutating the Runx binding sequence or by co-transfecting with Runx2 antisense expression plasmid,