ライフサイエンスコーパス: co-translational

1 of NatA, the major human NAT involved in the co-translational acetylation of proteins.

2 al membrane insertion of the tomato HMGRs is co-translational and does not involve cleavage of an N-t

3 the fundamental point of convergence between co-translational and post-translational endoplasmic-reti

4 gnal peptide breaks the alpha helix allowing co-translational cleavage.

5 Asparagine-linked glycosylation, the co-translational covalent attachment of carbohydrates to

6 CARSs also catalyze co-translational cysteine polysulfidation and are involv

7 Strikingly, co-translational decay targets encode proteins with high

8 tor (CFTR) is one ERAD substrate targeted to co-translational degradation by the E3 ligase RNF5/RMA1.

9 The co-translational degradation of apoB is controlled by fa

10 dicate that, as RNF5, RNF185 targets CFTR to co-translational degradation.

11 ture and function of the encoded proteins by co-translational effects, we sought to test whether rs68

12 ical activity of StAR and that this post- or co-translational event accounts, in part, for the immedi

13 st that translocation arrest with subsequent co-translational exposure to the cytosol provides an alt

14 that their primary function is to facilitate co-translational folding after synthesis of an autonomou

15 ing represents a trade-off between promoting co-translational folding and sterically shielding the na

16 .Proteins fold under mechanical force during co-translational folding at the ribosome.

17 tructural elements, act as gauges of protein co-translational folding by reducing ribosome speed when

18 ly considered to be sufficiently narrow that co-translational folding can begin only when specific se

19 tic model that calculates a protein domain's co-translational folding curve during synthesis using on

20 Our approach explains essential features of co-translational folding curves and predicts how varying

21 ional elongation of the protein chain during co-translational folding in the cell, where insertion is

22 N-terminal GAU codons may facilitate correct co-translational folding in vertebrates.

23 this model accurately predicts the course of co-translational folding measured in vivo for four diffe

24 fold under the cradle created by TF, but the co-translational folding of larger proteins is slowed do

25 These results suggest a pathway for the co-translational folding of repeat proteins and have imp

26 han specific codon identity-could coordinate co-translational folding of the encoded protein.

27 The results show that the co-translational folding of the WT pro-SP-C TM segment i

28 ypeptides fold efficiently by sequential and co-translational folding of their domains.

29 ase components) is sufficient for successful co-translational folding of two bacterial alpha-helical

30 ectroscopy to provide a basis for studies of co-translational folding on the ribosome of this immunog

31 es, that TF does not alter significantly the co-translational folding process of a small protein G do

32 Time-resolving structure formation during co-translational folding revealed different secondary an

33 During co-translational folding, the nascent polypeptide chain

34 rocesses, ranging from muscle contraction to co-translational folding.

35 r understanding key aspects of the nature of co-translational folding.

36 form of the OST is primarily responsible for co-translational glycosylation of the nascent polypeptid

37 h would support the conditions necessary for co-translational import.

38 train of Escherichia coli for site-specific, co-translational incorporation of phosphoserine into pro

39 que family of proteins, characterized by the co-translational incorporation of selenium as selenocyst

40 SelB is an elongation factor needed for the co-translational incorporation of selenocysteine.

41 Co-translational insertion and folding is thus spontaneo

42 o reach their sites by diffusion after their co-translational insertion in the rough endoplasmic reti

43 he direct simulation of trajectories for the co-translational insertion of arbitrary polypeptide sequ

44 thereby co-ordinating pigment delivery, the co-translational insertion of LH polypeptides and their

45 ochondrial ribosomes and plays a role in the co-translational insertion of mitochondria-synthesized p

46 of the ribosome with the insertase promotes co-translational insertion of nascent chains.

47 oM methylase and the pyl operon required for co-translational insertion of pyrrolysine into the activ

48 lts establish that SBP2 is essential for the co-translational insertion of Sec into selenoproteins.

49 Co-translational insertion of selenocysteine (Sec) into

50 Selenoprotein biosynthesis relies on the co-translational insertion of selenocysteine in response

51 Membrane enrichment is caused by co-translational insertion of signal peptides recognized

52 er synthesis of TMDs 1 and 2, and that after co-translational insertion of the remaining TMDs, redund

53 ognition particle (SRP) targets proteins for co-translational insertion through or into the endoplasm

54 e C-terminal region show specific defects in co-translational insertion, suggesting that the close co

55 t simulation for the mechanistic analysis of co-translational integration and for the engineering of

56 er supported by the fact that inhibiting the co-translational interaction of hdeltaOR-Cys(27) precurs

57 of apoB support the intrinsic capability of co-translational lipid recruitment.

58 cytosol and targeted to the proteasomes in a co-translational manner.

59 Here, we elucidate the co-translational mechanism responsible for the expressio

60 As a test for a co-translational mechanism, a chimera of GFP that contai

61 This co-translational membrane insertion and processing requi

62 During co-translational membrane insertion of membrane proteins

63 ribosome nascent chain complex, allowing for co-translational membrane insertion, whereas loop C1 may

64 verages a recently demonstrated link between co-translational membrane integration efficiency and pro

65 e dual signal chimera provides support for a co-translational mitochondrial import pathway.

66 ther, these data suggest a mechanism for the co-translational mode of YidC-mediated membrane protein

67 t some limited topological malleability, the co-translational model likely dominates under normal cir

68 A second, co-translational model, posits that the protein inserts

69 N-myristoylation is a co-translational modification occurring for members of t

70 The co-translational modification of N-terminal acetylation

71 Amino-terminal acetylation is a critical co-translational modification of the newly synthesized p

72 sting that the fragments may be generated by co-translational mRNA decay.

73 Co-translational mRNA degradation is a widespread proces

74 eat effects by inducing ribosome pausing and co-translational mRNA turnover.

75 ese findings are the first to establish that co-translational N-glycosylation of human GGT is require

76 we show that loss of DC2 causes a defect in co-translational N-glycosylation of proteins that mimics

77 ligosaccharyltransferase complex involved in co-translational N-glycosylation.

78 he protein translocation channel to catalyze co-translational N-linked glycosylation of proteins in t

79 ately 177 kDa ALK polypeptide core undergoes co-translational N-linked glycosylation, emerging in its

80 series of covalent modifications, including co-translational N-myristoylation at Gly(2), as well as

81 ese proteins cannot follow the SRP-dependent co-translational pathway that typifies most integral mem

82 transmembrane domain that cannot access the co-translational pathway.

83 l inner membrane proteins are assembled by a co-translational process directed by SRP/FtsY, the SecYE

84 his meant that an important dimension of the co-translational process remained unstudied.

85 n the ER, (b) heterodimer formation is not a co-translational process, and (c) heme insertion is a de

86 thesis, potentially influencing simultaneous co-translational processes such as folding and chemical

87 cleavage and N-glycosylation of proteins are co-translational processes, but little is known about th

88 pausing of ribosomes can affect a variety of co-translational processes, including protein targeting

89 vidence that PEXEL position is conserved for co-translational processing and export.

90 hionine aminopeptidase (MetAP) catalyzes the co-translational processing of initiator methionine from

91 e have assayed the 24-kD fl2 alpha-zein in a co-translational processing system in vitro.

92 Detailed structural studies of co-translational protein folding are now beginning to em

93 mic nascent chain associations to coordinate co-translational protein folding, facilitate accelerated

94 role in regulating translation kinetics and co-translational protein folding.

95 sage regulates protein function by affecting co-translational protein folding.

96 expression levels, ribosome localization and co-translational protein folding.

97 tors and suggest that the basic mechanism of co-translational protein targeting is conserved between

98 The SRP is a central component of the co-translational protein targeting machinery that binds

99 During co-translational protein targeting, the signal recogniti

100 lance between the efficiency and fidelity of co-translational protein targeting.

101 nfolded Protein Response is coupled with the co-translational protein translocation pathway to mainta

102 Sec61 is the key component of the mammalian co-translational protein translocation system and has be

103 access to bulk lipid, we propose a model for co-translational protein translocation.

104 elease the truncated protein product in this co-translational quality control pathway.

105 ger RNAs that lack stop codons is one of the co-translational quality control pathways.

106 modulate translation elongation and impacts co-translational quality control to minimize production

107 es and inserted into the inner membrane in a co-translational reaction facilitated by the Oxa1 insert

108 Studies in vivo show that co-translational regulation of the viral coat and replic

109 evailing view of N-terminal acetylation as a co-translational ribosome-associated process and suggest

110 mic domains requiring the dual action of the co-translational Sec and post-translational Tat pathways

111 enous opal suppression pathway competes with co-translational Sec insertion.

112 Co-translational secondary folding of nascent chains ins

113 Furthermore, rerouting of the scFab to the co-translational signal recognition particle pathway com

114 We here detail the mechanism by which co-translational signal-peptide cleavage is prevented.

115 wo distinct N-glycosylation sites: a typical co-translational site and a consensus site approximately

116 Mutations that ablate the co-translational site concomitantly reduce glycosylation

117 of the KCNE1 post-translational site into a co-translational site restored both monoglycosylation an

118 he Ssa family of cytosolic Hsp70 and not the co-translational Ssb homolog, consistent with the lack o

119 embranes is one of the central themes to the co-translational targeting and import of proteins.

120 an in vitro assay that faithfully mimics the co-translational targeting and translocation of the amin

121 nthesized triglyceride (TG) are critical for co-translational targeting of apolipoprotein B (apoB100)

122 (SRP) and its receptor (SR) function in the co-translational targeting of nascent protein-ribosome c

123 recognition particle (SRP) pathway mediates co-translational targeting of nascent proteins to membra

124 ribonucleoprotein particle that mediates the co-translational targeting of newly synthesized proteins

125 l recognition particle (SRP) is required for co-translational targeting of polypeptides to the endopl

126 ) and the SRP receptor (SR) that control the co-translational targeting of proteins to cellular membr

127 ntial component of the SRP that mediates the co-translational targeting of proteins to the correct ce

128 recognition particle (SRP) that functions in co-translational targeting of proteins to the membrane.

129 GTPases Ffh and FtsY play a central role in co-translational targeting of proteins, assembling in a

130 The first step in the co-translational targeting of secretory proteins to the

131 in from the precursor did not interfere with co-translational targeting of the nascent chain to the S

132 . coli components to function in a bona fide co-translational targeting pathway remains unclear.

133 In co-translational targeting, interactions among these pro

134 ase, an SP that is recognized by the SRP for co-translational targeting, it is found that substrate b

135 ligosaccharyl transferase (OT) catalyzes the co-translational transfer of a dolichol-linked tetradeca

136 y to demonstrate that Sec63p is required for co-translational translocation in vitro and specifically

137 e cytoplasm, rather than allowing its normal co-translational translocation into the endoplasmic reti

138 sttranslational direct chloroplast import or co-translational translocation into the ER prior to chlo

139 demonstrate that in vitro reconstitution of co-translational translocation is greatly enhanced using

140 d that this is due to its ability to inhibit co-translational translocation of CD4 into the lumen of

141 hat associates with ribosomes to promote the co-translational translocation of proteins across biolog

142 The co-translational translocation of proteins into the endo

143 During the co-translational translocation of secretory and membrane

144 These findings support the hypothesis that a co-translational translocation pathway exists for import

145 This co-translational translocation pathway is initiated when

146 associates with the translating ribosome in co-translational translocation, and with the SecA ATPase

147 In co-translational translocation, the ribosome funnel and

148 doplasmic reticulum membrane is important to co-translational translocation.

149 The magnitude of co-translational ubiquitination and subsequent degradati

150 described of these degradative processes is co-translational ubiquitinylation and proteasomal degrad