ライフサイエンスコーパス: coatomer

1 ontribute to stabilizing membrane-associated coatomer.

2 ion of the p23 cargo-protein-binding site on coatomer.

3 gain novel insights into the architecture of coatomer.

4 glycoside antibiotics that are known to bind coatomer.

5 at components including AP-2, AP180 and COPI coatomer.

6 dues of the KKXX motif that is known to bind coatomer.

7 of KIF5B and beta'-COP, a component of COP1 coatomer.

8 nt with ARF forming direct interactions with coatomer.

9 the AD7 antibody interacts with undenatured coatomer.

10 he Golgi membrane in molar excess over bound coatomer.

11 blocked the interaction of antibiotics with coatomer.

12 teracting with the di-lysine binding site of coatomer.

13 ch blocked the coating of Golgi membranes by coatomer.

14 T1, a gene that encodes the alpha subunit of coatomer.

15 rf1 bound to the gammazeta-COP subcomplex of coatomer.

16 nolayer and thus seem unsuitable targets for coatomers.

17 ctor for wild-type epsilon-COP, a subunit of coatomers.

18 r membrane and the subsequent recruitment of coatomer, a coat protein complex consisting of seven sta

19 two cytosolic components, ARF G protein and coatomer, a heptameric complex that can polymerize into

20 One well-studied example is COPI or coatomer, a heptameric protein complex that is recruited

21 also reveal an alternate mechanism by which coatomer acts.

22 from that predicted from the current model, coatomer affected the K(m) and not the k(cat) values.

23 fs on transmembrane proteins are captured by coatomer alpha-COP and beta'-COP subunits and packaged i

24 Purified coatomer also bound selectively to artificial lipid vesi

25 mbda and soluble components, including COPI (coatomer and ADP-ribosylation factor), results in the re

26 e propose and corroborate a simple model for coatomer and Arf1 activity based on results analysing th

27 We propose that this continuous activity of coatomer and Arf1 generates kinetically stable membrane

28 Rapid membrane binding and dissociation of coatomer and Arf1 occur stochastically, even without ves

29 ution and lifetime of fluorescently labelled coatomer and Arf1 on Golgi membranes of living cells.

30 These data suggest that ArfGAP1, coatomer and Arf1 play interdependent roles in the assem

31 ange dependent on engagement of ArfGAP1 with coatomer and Arf1, and affected by secretory cargo load.

32 We have isolated native yeast coatomer and examined its structure and subunit organiza

33 Membrane recruitment of coatomer and formation of coat protein I (COPI)-coated v

34 required for epsilon-COP incorporation into coatomer and maintainance of normal epsilon-COP levels.

35 eraction with the coatomer protein complex I coatomer and resulted in accumulation of GFP-tpn-aa in t

36 st three-dimensional picture of the complete coatomer and reveal substantial conformational flexibili

37 ics interact with di-lysine binding sites on coatomer and that coatomer contains at least two of thes

38 COPI, a protein complex consisting of coatomer and the small GTPase ARF1, is an integral compo

39 istinct vesicle coat complexes, termed COPI (coatomer) and COPII, whose assembly is regulated by the

40 including Sec18p, the Lma1p complex, Uso1p, coatomer, and Arf1p.

41 rgoes are the size of COPI vesicles, contain coatomer, and functionally require ARF1 and coatomer for

42 tion between the Golgi-vesicle coat protein, coatomer, and the regulatory GTP-binding protein Cdc42.

43 on of epsilon-COP, the assembly of COPs into coatomers, and the participation of coatomers in intrace

44 The Arf1-binding sites on coatomer are spatially related to PtdIns4,5P(2)-binding

45 chemically defined liposomes incubated with coatomer, Arf1p, and guanosine 5'-[gamma-thio]triphospha

46 To establish the initial site of coatomer assembly after export from the ER, we immunoiso

47 COPI phosphorylation may regulate coatomer assembly, membrane recruitment, or the specific

48 the distribution of the beta-COP subunit of coatomer because COPI partially localizes to pre-Golgi i

49 th IAP (inhibitor of apoptosis) or COP (COPI coatomer, beta subunit) dsRNA silenced their target gene

50 We suggest that Ist2 dimerization triggers coatomer binding and clustering of this protein into dom

51 om cytosolic pools, acts directly to promote coatomer binding and is in a 3:1 stoichiometry with coat

52 CBM inhibited coatomer binding to Golgi membranes in vitro and in vivo

53 contrast to the behavior of the COPII coat, coatomer binds to liposomes containing a variety of char

54 In the absence of Arf1p, coatomer binds to liposomes containing dioleoylphosphati

55 LD, rather than with ARF and endogenous PLD, coatomer bound to Golgi membranes.

56 r activating or inhibiting signaling through coatomer-bound Cdc42.

57 o donor membranes, leading to recruitment of coatomer, bud formation, and eventual vesicle release.

58 em, vaccinia formation bypasses this role of coatomer, but instead, depends on coatomer interacting w

59 oatomer to p24 tails suggests models for how coatomer can potentially package retrograde-directed and

60 ding to COPI vesicles whereas preventing the coatomer-Cdc42 interaction stimulates dynein binding.

61 athrin clusters or 2) retrograde cargoes and coatomer clusters.

62 port to the endoplasmic reticulum, contain a coatomer coat and that coatomer is required for their fo

63 by coatomer for selective uptake into COPI (coatomer)-coated vesicles.

64 but might involve dynamic processes such as coatomer-coated (COPI) vesicle-mediated trafficking.

65 ng kinetics, is more potent at uncoating the coatomer-coated membrane than EGTA, suggesting that a ca

66 When coatomer-coated membranes are incubated in the presence

67 Formation of coatomer-coated vesicles from Golgi-enriched membranes r

68 known to promote, by itself, scission of the coatomer-coated vesicles that mediate intra-Golgi transp

69 sicles and tubules slightly larger than COPI/coatomer-coated vesicles.

70 PA are key early events for the formation of coatomer-coated vesicles.

71 We identified the gamma-subunit of the coatomer complex (gammaCOP) as a specific binding partne

72 Immunodepletion of COPI coatomer complex and associated proteins from cytosolic

73 biosynthetic membrane transport by the COPI coatomer complex for efficient replication.

74 The heptameric coatomer complex forms the protein shell of membrane-bou

75 The dibasic motif bound the coatomer complex I (COPI) in an in vitro binding assay,

76 ein RPL35A, putative RNA helicase DDX24, and coatomer complex I (COPI) subunit ARCN1 most significant

77 Depletion of the proteins of the coatomer complex I or its upstream effectors ARF1 or GBF

78 Coatomer complex I was also required for infection of ly

79 opus extracts, we report a role for the COPI coatomer complex in nuclear envelope breakdown, implicat

80 ntains an interaction with its effector, the coatomer complex of COPI-coated vesicles.

81 ry of LF to the cytosol requires either COPI coatomer complex or a COPI subcomplex for translocation

82 nes requires an Arf family G protein and the coatomer complex recruited from cytosol.

83 d by the small GTPase Arf1 that recruits the coatomer complex to the membrane, triggering polymerizat

84 upershift strongly indicated that the entire coatomer complex was the trans-acting factor.

85 The COPI coatomer complex, which plays a major role in membrane r

86 if at the C-terminus of Sac1 is required for coatomer complex-I (COP-I)-binding and continuous retrie

87 psilon-COP is to stabilize alpha-COP and the coatomer complex.

88 h at least zeta-COP and beta-COP of the COPI coatomer complex.

89 d by the COPI budding machinery ARF1 and the coatomer complex.

90 imperfect, caused by mutations in the COPII coatomer complex.

91 ilar to a region in the beta-subunit of COPI coatomer complexes, which suggests the existence of a sh

92 We conclude that Arf1p-GTP and coatomer comprise the minimum apparatus necessary to cre

93 Coatomer consists of two subcomplexes: the membrane-targ

94 All of the antibiotics that interacted with coatomer contain at least two close amino groups, sugges

95 di-lysine binding sites on coatomer and that coatomer contains at least two of these di-lysine bindin

96 ivate phospholipase D1 (PLD1), (iii) recruit coatomer (COP-I) to Golgi-enriched membranes, and (iv) e

97 hosphoinositide binding selectivity of Golgi coatomer COPI polypeptides was examined using photoaffin

98 y transient interaction between DRD3 and the coatomer COPI, a complex involved in membrane transport,

99 ly free of the classical Golgi coat proteins coatomer (COPI) and clathrin.

100 The coatomer (COPI) complex mediates Golgi to ER recycling o

101 of the adaptor protein 1 (AP-1) complex and coatomer (COPI) onto these membranes and activates phosp

102 hought to play a critical role in recruiting coatomer (COPI) to Golgi membranes to drive transport ve

103 One pool of actin cofractionates with coatomer (COPI)- coated vesicles and is sensitive to sal

104 By analogy to the coatomer (COPI)-independent transport of Golgi enzymes t

105 er, a cytosolic protein fraction depleted of coatomer could not support vesicle formation but it did

106 This study shows that coatomer couples sorting signal recognition to the GTP h

107 Strikingly, the alphabeta'-COP core of coatomer crystallizes as a triskelion in which three cop

108 ytoplasmic signal sequence of hp24a inhibits coatomer-dependent GTP hydrolysis.

109 d in ArfGAP1 being trapped on the Golgi in a coatomer-dependent manner.

110 olgi membranes was also investigated using a coatomer-dependent vesicle budding assay.

111 Biochemical experiments show that coatomer directly participates in the GTPase reaction, a

112 er with the previously established ancestral coatomer element (ACE1), these two elements constitute t

113 Ancestral coatomer element 1 (ACE1) proteins assemble latticework

114 conserved tripartite element, the ancestral coatomer element ACE1, that reoccurs in several nucleopo

115 w a surprising way by which vaccinia hijacks coatomer for early viral biogenesis.

116 signals on cargo proteins are recognized by coatomer for selective uptake into COPI (coatomer)-coate

117 coatomer, and functionally require ARF1 and coatomer for transport.

118 Whereas coatomer forms COPI vesicles in the host early secretory

119 We report herein that neomycin precipitates coatomer from cell extracts and from purified coatomer p

120 Moreover, isolation of coatomer from metabolically labeled tissue culture cells

121 Mutant coatomer from sec28 Delta cells behaves as an unusually

122 purify the cytosolic COPI precursor complex, coatomer, from rat liver cytosol.

123 ensable for yeast cell viability and overall coatomer function, but is required for KKXX-dependent tr

124 he ER and its formation requires ER exit and coatomer function.

125 Coatomer functions in binding and sequestering cargo mol

126 When SEC28 and other coatomer genes were mutated, FBPase degradation was defe

127 ociation with Vid vesicles was impaired when coatomer genes were mutated.

128 nal selection is under kinetic control, with coatomer governing a GTPase discard pathway that exclude

129 tomer into large aggregates and implies that coatomer has two or more binding sites for neomycin.

130 which competes for the same binding site on coatomer, has no effect on GTPase activity.

131 er, once associated with membranes, Arf1 and coatomer have different residence times: coatomer remain

132 Vesicle coat proteins, including coatomer-I subunits, localize to and are required for th

133 This vesicle does not require coatomer-II (COPII) proteins for budding from the ER mem

134 Vesicles bound coatomer in a physiological fashion requiring an ARF1-gu

135 ARF is at most a minor component relative to coatomer in coated vesicles from all cell lines tested,

136 of GFP-tagged versions of ArfGAP1, Arf1, and coatomer in living cells.

137 hypothesis that these RGS proteins sequester coatomer in the cytoplasm and inhibit its recruitment on

138 Glo3p also interacts with intact coatomer in vitro.

139 Despite depressed binding of coatomer in vivo, the Golgi complex retained its charact

140 mechanism previously documented for Arf GAP1/coatomer in which Arf1 is inactivated in a tripartite co

141 OPs into coatomers, and the participation of coatomers in intracellular membrane transport.

142 redistribution of the vesicle coat protein, coatomer, in the cell.

143 We conclude that prolonged coatomer inactivation perturbs cellular endocytic transp

144 t which replication stage(s) was affected by coatomer inactivation, we used visual and biochemical as

145 Cdc42, when bound to coatomer, inhibits dynein binding to COPI vesicles where

146 entify links between them that contribute to coatomer integrity.

147 is role of coatomer, but instead, depends on coatomer interacting with the host KDEL receptor.

148 ng mode has implications for the dynamics of coatomer interaction with the Golgi and for the selectio

149 This suggested that neomycin cross-linked coatomer into large aggregates and implies that coatomer

150 We conclude that coatomer is an allosteric regulator of Arf GAP1.

151 Our results offer an explanation of why COPI coatomer is frequently identified in screens for cellula

152 Coatomer is know to interact with a motif (KKXX) contain

153 RNA interference experiments confirm that coatomer is required for cER induction in vivo, as are m

154 reticulum, contain a coatomer coat and that coatomer is required for their formation.

155 Coatomer is the soluble precursor of the COPI coat (coat

156 e complex of Coat Protein I (COPI), known as coatomer, is sufficient to induce coated vesicular-like

157 This role for Arf1/coatomer might provide a model for investigating the beh

158 In evolution, a single coatomer module increases in size from hetero-heptamer (

159 The coatomer module of the nuclear pore complex borders the

160 about the evolution and the assembly of the coatomer module of the nuclear pore complex.

161 acent to the vacuolar membrane in the ret2-1 coatomer mutant.

162 ed to be inefficient in the arf mutants, and coatomer mutants with no detectable anterograde transpor

163 r binding and is in a 3:1 stoichiometry with coatomer on coated vesicles.

164 ther aminoglycoside antibiotics precipitated coatomer, or if they did not precipitate, they interfere

165 membrane recruitment, or the specificity of coatomer-organelle interaction.

166 oatomer from cell extracts and from purified coatomer preparations.

167 d to initiate the formation of clathrin- and coatomer protein (COP) I-coated vesicles on these membra

168 her H89 might act at the level of either the coatomer protein (COP)I or the COPII coat protein comple

169 evidence has suggested that subunits of the coatomer protein (COPI) complexes are functionally assoc

170 COPZ1 encodes a subunit of coatomer protein complex 1 (COPI) involved in intracellu

171 recombinant SBP-AR and the ligand-sensitive coatomer protein complex I (COPI) retrograde trafficking

172 the addition of cytosolic proteins including coatomer protein complex I (COPI) to the reaction mixtur

173 In this report, we identify the coatomer protein complex I (COPI) vesicle coat as a crit

174 This mutation abolished interaction with the coatomer protein complex I coatomer and resulted in accu

175 tivating protein (SCAP).SREBP-1c complex for coatomer protein complex II (COPII) vesicles.

176 the C1 cassette or by the presence of a PDZ/coatomer protein complex II-binding domain in the C2' ca

177 COPI is a coatomer protein complex responsible for intracellular p

178 ynthetic T1 peptides the specific binding of coatomer protein complex subunit beta to this region of

179 ction-based genomic screening identified the coatomer protein complex zeta1 (COPZ1) gene as essential

180 of COPZ1, but not of COPZ2 encoding isoform coatomer protein complex zeta2, caused Golgi apparatus c

181 FLNB (filamin B, beta) and hypoedited COPA (coatomer protein complex, subunit alpha).

182 ion in a WD40 domain of the highly conserved Coatomer Protein Complex, Subunit Beta 2 (COPB2).

183 140-kDa protein was the alpha-COP subunit of coatomer protein COPI, usually associated with trans-Gol

184 The COPa and COPb proteins of the coatomer protein I (COPI) vesicle complex were reported

185 Inhibition of the putative coatomer protein I (COPI) vesicle tethering complex, gia

186 Based on recent data showing that coatomer protein I (COPI) vesicle transport is involved

187 in the hKOPR C-tail decreased interaction of coatomer protein I (COPI) with the hKOPR and abolished 1

188 tif was responsible for the interaction with coatomer protein I (COPI), which was inversely correlate

189 the cell surface expression by mediating the coatomer protein I complex-dependent retrograde transpor

190 (ER) and must be transported to the Golgi in coatomer protein II (COPII) vesicles where two sequentia

191 protein, which is an essential component of coatomer protein II (COPII)-mediated cargo transport fro

192 d that decreased expression of the gammaCOPI coatomer protein led to 89% mortality in blood-fed mosqu

193 ctive RACK, which was identified as the COPI coatomer protein, beta'-COP.

194 embles classic coat protein complex I (COPI) coatomer protein-binding KKXX signals, and indeed the di

195 ER) is mediated by the SEC24C isoform of the coatomer protein-II complex.

196 alpha-adaptin; clathrin heavy chain; or beta-coatomer protein.

197 crosomes and partitions with subunits of the coatomer proteins that coat ER-to-Golgi transport vesicl

198 Coatomer proteins were identified as components of Vid v

199 COP subunit of COPI (coat protein complex I) coatomer proteins.

200 primary target for the effects of calcium on coatomer recruitment is a step that occurs after ADP-rib

201 Arf is also required with coatomer-related clathrin adaptor complexes to bud vesic

202 and coatomer have different residence times: coatomer remains on membranes after Arf1-GTP has been hy

203 Coatomer responds differently to different signals.

204 Although coatomer shares a common evolutionary origin with COPII

205 Coatomer stimulated Arf GAP1 activity; however, differen

206 ilon-COP and beta-, gamma-, delta-, zeta-COP coatomer subcomplexes and identify links between them th

207 eterious variants in the COPA gene (encoding coatomer subunit alpha) affecting the same functional do

208 ilar to that of brefeldin A, except that the coatomer subunit beta-COP remained on Golgi-derived memb

209 zygous mutations in ARCN1, which encodes the coatomer subunit delta of COPI.

210 and mitochondrial inheritance and for a COPI coatomer subunit in the targeting of a type V myosin to

211 steady state levels in ldlF cells of another coatomer subunit, beta-COP, and the peripheral Golgi pro

212 Mono-Q fraction as well as that of a second coatomer subunit, beta-COP.

213 using dsRNA directed against five other COPI coatomer subunits (alpha, beta, beta', delta, and zeta).

214 Among these proteins, several COPI coatomer subunits (alpha, beta, gamma, and delta) are of

215 we demonstrate novel direct interactions of coatomer subunits with regulatory proteins: beta'- and g

216 at both beta-COP and delta-COP, but no other coatomer subunits, were serine-phosphorylated.

217 Clathrin adaptor and COP-I coatomer subunits, which function in vesicle coat assemb

218 by the activities of the Sar1-COPII and Arf1-coatomer systems.

219 tative cargo receptors were shown to bind to coatomer, the coat protein of COPI-coated transport vesi

220 a combination of purified kinases, ARF1 and coatomer, the Golgi membranes were completely fragmented

221 ristoylated ARFs promoted the recruitment of coatomer to about the same extent.

222 on has been implicated in the recruitment of coatomer to Golgi membranes and release of nascent secre

223 glycoside antibiotics inhibit the binding of coatomer to Golgi membranes in vitro.

224 The enhanced recruitment of coatomer to membrane was specific to the Rab2 (13-mer) a

225 We find that activated Arf1 brings coatomer to membranes.

226 The either-or bimodal binding of coatomer to p24 tails suggests models for how coatomer c

227 branes with mitotic cytosol or with purified coatomer together with wild type ARF1 or its constitutiv

228 om the ER, whereas Golgi matrix proteins and coatomer undergo constant, rapid exchange between membra

229 tion factor (ARF) is absolutely required for coatomer vesicle formation on Golgi membranes but not fo

230 sicle formation but it did so after purified coatomer was added.

231 the antibody to beta-COP confirmed that the coatomer was the sole protein binding to the ASGR mRNA 5

232 alpha-, beta'-, and epsilon-COP subunits of coatomer, whereas other p24 domains bound the beta-, gam

233 e that this process does not require ARF and coatomers, which are necessary for the formation of Golg