ライフサイエンスコーパス: cobinamide

1 0) hydroxoaquocobinamide (hereinafter called cobinamide).

2 ile method to measure cyanide in blood using cobinamide.

3 a facile method for producing and purifying cobinamide.

4 everal commercially available cobalamins and cobinamide.

5 wn de novo intermediate, and did not salvage cobinamide.

6 ll depended on the assimilation of exogenous cobinamide.

7 gh binding affinity for the cobalamin analog cobinamide.

8 d for cob(II)alamin, base-off cobalamins, or cobinamides.

9 Cobalamin (1.4%) and cobinamide (1.8%) (an incomplete corrinoid) represented

10 Cobinamide, 5', 6'-dimethylbenzimidazole, and 2-aminopro

11 is in Drosophila melanogaster, we found that cobinamide, a B(12) analog and an effective NO scavenger

12 We showed previously that cobinamide, a cobalamin (vitamin B12) precursor, binds N

13 on the electronic properties of Cbls is Ado-cobinamide (AdoCbi(+)), an AdoCbl derivative that lacks

14 er preformed B(12) or two of its precursors, cobinamide and DMB.

15 appeared to convert most of the cobalamin to cobinamide and the 4 analogues that contain the bases-2-

16 Cobinamide appeared to act via scavenging NO because it

17 inity, and we now evaluated the potential of cobinamide as a NO scavenger in biologic systems.

18 human HC in complex with cyanocobalamin and cobinamide at 2.35 and 3.0 A resolution, respectively.

19 Cobinamide augmented the effect of an antibiotic and was

20 The vitamin B(12) precursor cobinamide binds cyanide with high affinity, changing co

21 ossible to detail the activities of purified cobinamide biosynthesis (Cbi) proteins CbiF, CbiG, CbiD,

22 ux through the 5,6-dimethylbenzimidazole and cobinamide (Cbi) activation branches of the nucleotide l

23 owing conversion of the incomplete corrinoid cobinamide (Cbi) into AdoCbl.

24 Cobinamide (Cbi) salvaging is impaired, but not abolishe

25 nents of two distinct pathways for salvaging cobinamide (Cbi), a precursor of adenosylcobalamin (AdoC

26 se distinct pathways for salvaging exogenous cobinamide (Cbi), a precursor of adenosylcobalamin (coen

27 importing an "incomplete" corrinoid, such as cobinamide (Cbi), and adding appropriate alpha- and beta

28 d cobalamin (Cbl) as a substrate better than cobinamide (Cbi; a Cbl precursor); the beta phosphate of

29 terest, we also studied reactions of NO with cobinamide [Cbi] in the 2+ and 3+ oxidation states.

30 A novel cyanide analyzer based on sensitive cobinamide chemistry relies on simultaneous reagent and

31 present a new method for the preparation of cobinamide (CN)2Cbi, a vitamin B12 precursor, that shoul

32 also utilize the incomplete corrinoid Co (1+)cobinamide (Co (1+)Cbi) as an alternative substrate.

33 tronic excited states of Co(2+)Cbl and Co(2+)cobinamide (Co(2+)Cbi(+), a cobalamin derivative that la

34 (2-pic) and pyridine (py) adducts of Co(II) cobinamide (Co(II)Cbi(+)), adducts thought to have long

35 Cobinamide did not inhibit the in vitro activity of eith

36 In rodent and human cells, cobinamide exhibited toxicity at concentrations > or =50

37 t CobU requires a nucleoside upper ligand on cobinamide for substrate recognition, with the nucleosid

38 e assimilation of exogenous unphosphorylated cobinamide from its environment.

39 paper reports evidence that archaea salvage cobinamide from the environment by using a pathway diffe

40 osynthesis pathway, but efficiently salvaged cobinamide from the environment, suggesting the existenc

41 The auxotrophic requirement for cobinamide-GDP was corrected when a wild-type allele of

42 allele of the cobY gene was auxotrophic for cobinamide-GDP, a known intermediate of the late steps o

43 Combining cobalamin with cobinamide had no effect on the ability of cobinamide to

44 The ability of archaea to salvage cobinamide has been under question because archaeal geno

45 dative stress in cells and tissues, and that cobinamide has promise as a first specific treatment for

46 are consistent with a salvaging pathway for cobinamide in which an amidohydrolase enzyme cleaves off

47 ongly support the proposal that the relevant cobinamide intermediates during de novo adenosylcobalami

48 We conclude that cobinamide is an effective intra- and extracellular NO s

49 noid substrates that are believed to include cobinamide, its precursor cobyric acid and probably othe

50 Compared to cobalamin, cobinamide lacks the tethered 5,6-dimethylbenzamidazolyl

51 Moreover, feeding flies cobinamide markedly attenuated subsequent NO-induced inc

52 Cobinamide on the filter changes color from orange (lamb

53 Alkaline cobinamide (orange, lambda(max) = 510 nm) changes to vio

54 We found that cobinamide reversed NO-stimulated fluid secretion in Dro

55 The vitamin B12 analog cobinamide reversed the cellular toxicity of sulfide, an

56 Cobinamide's mechanism of action appeared to be from red

57 The latter activity is required for cobinamide salvaging in bacteria.

58 B mutants suggested that the entry point for cobinamide salvaging is adenosylcobyric acid.

59 of color change increases 10 times when the cobinamide solution for filter impregnation is prepared

60 sphate guanylyltransferase needed to convert cobinamide to adenosylcobinamide-GDP during the late ste

61 h cobinamide had no effect on the ability of cobinamide to scavenge NO.

62 not presence, of cobalamin, suggesting that cobinamide toxicity was secondary to interference with c

63 Cobinamide was taken up efficiently by cultured rodent c

64 the cobalt in the lower axial position, and cobinamide, which lacks the dimethylbenzimidazole nucleo

65 Lastly, an RNA aptamer for cobinamide, which was originally selected in the presenc

66 yme B(12) de novo and from Cby, but not from cobinamide, which was the expected phenotype for an L-th

67 Cobinamides, with the B(12) 5,6-dimethylbenzimidazole lo

68 Cobinamide worked through two distinct mechanisms: direc

69 Here we show that adding sodium nitrite to cobinamide yields a stable derivative (referred to as ni