ライフサイエンスコーパス: colipase

1 rols, exist independently of the presence of colipase.

2 to the putative PTL binding domains of human colipase.

3 es smaller than the area occupied by a bound colipase.

4 o tributyrin to the same extent as wild type colipase.

5 monolayers and emulsions and for binding to colipase.

6 s to change the interaction between hPTL and colipase.

7 5-fold less activity compared with wild-type colipase.

8 to the monolayer of the amphipathic protein, colipase.

9 novel structure involving fatty acid and/or colipase.

10 nction of pancreatic triglyceride lipase and colipase.

11 but shows little additional interaction with colipase.

12 ce, even if procolipase is added before [14C]colipase.

13 Colipase, a cofactor of pancreatic triacylglycerol lipas

14 Colipase adsorbs to a level of 28-30 pmol/cm2 to form a

15 Paradoxically, reactants lowered colipase adsorption rates only if phosphatidylcholine wa

16 is further developed and applied to analyze colipase adsorption rates to mixed monolayers of phospho

17 Colipase adsorption rates to phosphatidylcholine/reactan

18 colipase ratios >25 induces higher levels of colipase adsorption than at lower ratios.

19 It is successfully applied to rate data for colipase adsorption to phospholipid alone and yields rea

20 actants create dynamic complexes that impede colipase adsorption.

21 interface or both, influence the binding of colipase and hPTL through interactions with the beta5'-l

22 ray crystal structure of the complex between colipase and lipase suggest another function for colipas

23 function of the interaction between Glu15 of colipase and lipase, we examined one mutant, E15R, in de

24 If [14C]colipase and procolipase are premixed for > 12 h at pH a

25 e may regulate the type of surfaces to which colipase and, hence, lipase bind and may control the spe

26 ase and trypsin, and mRNAs for chymotrypsin, colipase, and others that may derive from uninfected epi

27 human pancreatic triglyceride lipase and of colipase, another pancreatic protein that interacts with

28 py suggests that the phosphatidylcholine and colipase are miscible in the interface.

29 his hypothesis by introducing mutations into colipase at position 15, a residue that contacts the lid

30 Colipase binding rate depends nonlinearly on the two-dim

31 The initial rate of colipase binding to fluid, single-phase lipid monolayers

32 in proportion to their activity, each mutant colipase bound to tributyrin to the same extent as wild

33 yer differs from the interaction of the hPTL-colipase complex with a dicaprin monolayer or a triglyce

34 s for PTL and prevented the formation of PTL.colipase complexes.

35 These results support the hypothesis that colipase concentrates fatty acids laterally at its perip

36 Colipase does not significantly influence the conformati

37 er of either or both lipids is present, [14C]colipase dominates the adsorption process, even if bile

38 the fatty acid-rich nano-domain surrounding colipase facilitates lipase adsorption in the 'flap-open

39 ancreatic triglyceride lipase (PTL) requires colipase for activity.

40 y as a consequence of the higher affinity of colipase for such interfaces.

41 trates and by dependence on another protein, colipase, for binding to the substrate interface.

42 Presumably, colipase functions by anchoring and orienting PTL at the

43 n most models of pancreatic lipase activity, colipase functions to anchor lipase on the substrate int

44 pase-lipase complex at an interface and that colipase has a function in lipolysis in addition to anch

45 In the x-ray structure, colipase has a hydrophobic surface positioned to bind su

46 tion of the lipase cofactor, procolipase, to colipase has no consequence for intestinal lipolysis and

47 In the presence of bile salt micelles and colipase, human PLRP2 hydrolyzed long-chain tri-, di-, a

48 ry to the existing paradigm, the presence of colipase in a lipid monolayer was not sufficient to enab

49 According to this model, the presence of colipase in an interface should be sufficient to enable

50 pase and lipase suggest another function for colipase in maintaining the active conformation of lipas

51 With saturating [14C]colipase in the subphase, the surface excess of [14C]col

52 If so, by analogy with the behavior of colipase, increasing diacylglycerol may not trigger tran

53 phery and suggest that, together with lipase-colipase interaction, the fatty acid-rich nano-domain su

54 demonstrate that the hydrophilic surface of colipase interacts with PTL in solution to form active c

55 in the subphase, the surface excess of [14C]colipase is 29% higher than that of procolipase, indicat

56 Colipase is a cofactor protein which forms a 1:1 complex

57 e that Glu15 is critical for activity of the colipase-lipase complex at an interface and that colipas

58 ity demonstrated in this study suggests that colipase may regulate the type of surfaces to which coli

59 colipase was dependent on the fatty acid and colipase mole fractions.

60 rically over this range of compositions, the colipase molecules should be separated by up to 0-2 acyl

61 the mutations decreased the affinity of the colipase mutants for PTL and prevented the formation of

62 hexapeptide fused to the carboxyl domains of colipase or dickkopf are devoid of biological activity.

63 Other studies suggest that colipase or its proform, procolipase, may have additiona

64 er than that of procolipase, indicating that colipase packs more tightly in the interface.

65 Presumably, colipase performs the same function in vivo, but little

66 To test this hypothesis, mixed monolayers of colipase, phosphatidylcholine, and fatty acid at the arg

67 With [14C]colipase-procolipase mixtures, the proteins compete equa

68 The x-ray structure of the colipase.PTL complex supports this model.

69 nteracts with PTL in solution to form active colipase.PTL complexes, that bile salt micelles influenc

70 trongly with all lipids when the lipid chain:colipase ratio is </=3.

71 ved with the fatty acid which at lipid chain:colipase ratios >25 induces higher levels of colipase ad

72 also remains in the interface at lipid chain:colipase ratios >3 but shows little additional interacti

73 At lipid chain:colipase ratios >3, the triacylglycerol is excluded from

74 At higher lipid chain:colipase ratios, diacylglycerols are likely excluded fro

75 the monolayer phase up to </=25 lipid chain:colipase ratios.

76 Colipase, reg-1, C-reactive protein-ductin, and amyloid

77 Spread colipase remained associated with the lipid monolayer in

78 ample, with diacylphosphatidylcholine alone, colipase remained in the lipid monolayer at surface pres

79 Colipase restores activity to lipase in the presence of

80 yceride lipase (PTL) showed that there was a colipase-stimulated REH activity in rat and mouse (WT an

81 Surface pressure and colipase surface concentration were measured as a functi

82 s tested by measuring the adsorption of [14C]colipase to monolayers of 1-stearoyl-2-oleoyl-sn-3-glyce

83 tro, pancreatic triglyceride lipase requires colipase to restore activity in the presence of inhibito

84 orption over that observed in the absence of colipase was dependent on the fatty acid and colipase mo

85 Multiple mutant colipases were expressed and shown to have decreased act

86 nteraction of the pancreatic lipase cofactor colipase with a diacylphosphatidylcholine, acylglycerols

87 binding, and that the proper interaction of colipase with PTL requires the Glu(64)/Arg(65) binding s

88 E15R was as active as wild-type colipase with these mutant lipases.