ライフサイエンスコーパス: comet assay

1 lar zinc increased DNA single-strand breaks (comet assay).

2 horesis and single cell gel electrophoresis (Comet Assay).

3 ononuclear cells from 13 pSS patients, using comet assay.

4 eased after IGF-IR activation as measured by comet assay.

5 doses of 0.1 to 1.5 kGy and analyzed by DNA Comet Assay.

6 ibutable to DNA damage, as determined by the comet assay.

7 om TPZ-induced DNA damage measured using the comet assay.

8 ed from measurements of DNA damage using the comet assay.

9 -/-)p53(-/-) MEFs as demonstrated by neutral Comet assay.

10 assessed DNA damage by means of the neutral comet assay.

11 T3-L1 normal cells was detected by using the comet assay.

12 ential induced DNA damage in vitro using the Comet Assay.

13 dants and the DNA damage was measured by the Comet assay.

14 xtracts induced DNA damage, evaluated with a comet assay.

15 llowing gamma-irradiation as assessed by the comet assay.

16 nternalization, as evaluated by the alkaline comet assay.

17 sed DNA double-strand breaks as shown by the comet assay.

18 ated with accelerated DNA repair measured by comet assay.

19 gammaH2A.X expression/foci formation and by comet assay.

20 mmunolocalization of gamma-H2AX foci and the COMET assay.

21 ity to repair DNA lesions as measured by the comet assay.

22 unhooking capacity measured using a modified comet assay.

23 trand breaks, as reflected in gammaH2A.X and comet assays.

24 estern blot analysis, immunofluorescence and comet assays.

25 e repairs, as shown by radio-sensitivity and Comet assays.

26 rm of histone H2AX (gamma-H2AX) and alkaline Comet assays.

27 he alkaline single cell gel electrophoresis (comet) assay.

28 by using a single-cell gel electrophoresis (comet) assay.

29 beling, and single-cell gel electrophoresis (COMET) assays.

30 ubsequently measured as DNA breaks using the comet assay (7 groups of volunteers, in six countries).

31 ncluding DNA damage in lymphocytes (with the comet assay), activity of detoxifying enzymes (glutathio

32 tion of the single cell gel electrophoresis (comet) assay after administration of a therapeutic dose.

33 Compared with halo assay or comet assay alone, this method allows automated analysis

34 Clonogenic survival assays and comet assays also show that Spy1 expression enhances cel

35 Using both a modified alkaline comet assay and a DNA cleavage assay, we demonstrate tha

36 breaks (DSBs) that are detectible by neutral COMET assay and as gamma-H2AX foci that colocalize with

37 firming this phenomenon, we have revealed by comet assay and currently the most sensitive method of D

38 yed ICL processing as revealed by a modified Comet assay and gamma-H2AX foci persistence.

39 nhanced DNA damage, as measured by using the comet assay and gammaH2AX staining.

40 he offspring, stability was assessed via the Comet assay and highly sensitive, semiautomated confocal

41 DNA damage following BPA exposure using the comet assay and immunofluorescence staining, and used ce

42 898E-reconstituted cells, as revealed by the comet assay and IR-induced Rad51 foci formation assay.

43 induced DSBs as revealed by both the neutral comet assay and measurements of the specific DNA damage

44 This study demonstrates, by using neutral comet assay and pulsed field gel electrophoresis, that h

45 Here, we confirm by specific comet assay and pulsed-field electrophoresis that cells

46 ine-dependent DNA damage, as measured by the comet assay and the expression of the phosphorylated for

47 Moreover, as measured by the Comet assay and the induction of gamma-H2A.X, quercetin,

48 h was associated with DNA damage analyzed by comet assay and up-regulation of p53.

49 An Alu retrotransposition assay, COMET assays and 53BP1 foci staining show that the SpORF

50 ged the induction of DNA damage as judged by Comet assays and gammahistone 2AX (gammaH2AX) and checkp

51 emcitabine-induced DNA damage as measured by comet assays and quantification of gammaH2AX foci.

52 DNA repair as revealed by gammaH2AX foci and comet assays and to the up-regulation of Ku70 and DNA-de

53 in single-strand breaks (as measured by the comet assay) and 7,8-dihydro-8-oxo-2'-deoxyguanosine (8-

54 DNA single-strand breaks (SSBs) (assayed by comet assays) and ATM autophosphorylation (at Ser-1981).

55 ense and damage, biomarkers of genotoxicity (comet assay), and behavioral biomarkers (feeding and bur

56 duced DNA strand breaks in the hOGG1-coupled comet assay as well as 8-oxo-dGuo (as measured by LC-ESI

57 , VP-16-induced DSBs as monitored by neutral comet assay, as well as DNA damage signals (e.g. gamma-H

58 NA damage responses, as evidenced by neutral comet assay, as well as H2AX, Chk2 and p53 phosphorylati

59 but induced DNA damage, as determined by the comet assay, at all concentrations tested.

60 tivation, spontaneous DNA damage by alkaline comet assay, basal micronuclei levels, the number of lar

61 mical analysis of gammaH2AX focus formation, comet assay-based DNA strand break analysis, and measuri

62 essed on peripheral blood lymphocytes by the comet assay before and after exposing cells to a tobacco

63 The Comet assay (CA) is a sensitive/simple measure of genoto

64 he alkaline single cell gel electrophoresis (comet) assay can be combined with fluorescent in situ hy

65 Single cell gel electrophoresis (the comet assay), continues to gain popularity as a means of

66 This modified comet assay could represent a stand-alone test or an adj

67 Comet assays demonstrated that DNA repair was delayed in

68 Additionally, a neutral and alkaline comet assay displayed a persistent level of intrinsic DN

69 lated using published potencies based on the comet assay for Chinese hamster ovary cells (assesses th

70 0) and lethal dose (LD(50)), Ames tests, and Comet assays for in vitro DNA damage.

71 utagen-sensitivity assay) and DNA damage (by comet assay) for individuals in higher-risk subgroups am

72 Our high-throughput variant of the comet assay greatly increases the number of samples anal

73 all three groups were used for the alkaline comet assay in the presence and absence of catalase.

74 onitored by formation of gamma-H2AX foci and comet assay, in an ATM (ataxia-telangiectasia mutated)-d

75 Sources of variability in the comet assay include variations in the protocol used to p

76 gammaH2AX IR-induced foci (IRIFs) and comet assays indicated ineffective NHEJ DNA repair in p1

77 Comet assays indicated that strand breaks accumulated, a

78 ct of Usp and Imu1-3 was assessed by MTT and Comet assays, infection assays, caspase 3/7 activity, fl

79 DNA damage as measured by the comet assay is associated with the development of multip

80 Thus, DNA Comet Assay may be a practical quarantine control method

81 ts with pediatric SLE, including the neutral comet assay (NCA), colony survival assay (CSA), irradiat

82 Comet assay of epithelial cells obtained from scraping t

83 aser scanning cytometer and then perform the comet assay on the same cells.

84 absence of DNA damage, detectable by either comet assay or 53BP1 focus formation.

85 diol epoxide-induced Olive tail moments, the comet assay parameter for measuring DNA damage, were sig

86 oach is a significant advance on the current comet assay procedure.

87 her levels of DNA damage, as assessed by the comet assay, quantification of 8-oxoguanine, and poly(AD

88 correlated very well with rodent 1/TD50 and Comet assay results.

89 The comet assay showed that tiron's protective effect agains

90 lkaline single-cell gel electrophoresis (the comet assay), significantly decreased after consumption

91 A modified version of the comet assay (single cell gel electrophoresis) was used t

92 Using the alkaline comet assay (single-cell gel electrophoresis), we observ

93 including active RAS pulldown, reporter and Comet assays, small interfering RNA-mediated depletion o

94 trand DNA breaking has been observed through comet assay technique.

95 DNA damage was quantified using the Comet assay, telomere DNA length by Southern blotting an

96 ion and genetic instability, as indicated by comet assays that showed increased numbers of DNA-strand

97 etermined the extent of DNA damage using the comet assay, the micronuclei assay, and the gamma-H2AX i

98 Compared with the microwell array based comet assay, this method can selectively capture and ana

99 By coupling the comet assay to 8-oxoguanine glycosylase (hOGG1), which e

100 Using the comet assay to detect DNA damage, we find that reoxygena

101 n combination with DNA methylation sensitive comet assay to determine the effects of vitamin B12 or M

102 cancer and 153 healthy controls, we used the comet assay to investigate the roles of cell cycle check

103 Using the single cell gel electrophoresis (comet) assay to detect DNA strand breaks in murine hepat

104 g the single cell gel electrophoresis assay (comet assay) to assess DNA damage.

105 We used single-cell gel electrophoresis (comet assay) to detect DNA-SSB in motor neurons.

106 et had lymphocyte DNA damage, as assessed by COMET assay, twice that found when they were fed the con

107 NO-dependent DNA damage as assessed by the comet assay was demonstrated during exposure of the thre

108 The comet assay was used to analyse the DNA damage.

109 The neutral comet assay was used to determine whether initial drug-i

110 The specificity of the comet assay was validated by coupling it to human 8-oxo-

111 on of FPG-sensitive sites, measured with the comet assay, was 0.34 per 10(6) guanines.

112 Using comet assays, we demonstrate that LT induces overt DNA d

113 tion of the single-cell gel electrophoresis (Comet) assay, we have measured formation and repair of D

114 foci; DSB levels were determined by neutral comet assay; western blotting was used to evaluate prote

115 maged rapidly, within 12 h, as revealed by a comet assay, which detects broken DNA, and by staining f

116 idual cells by alkaline gel electrophoresis (comet assay), with and without the addition of the repai

117 -induced DNA strand breaks using an alkaline comet assay (+/-z-VAD-fmk cotreatment) and by levels of