ライフサイエンスコーパス: complementation

1 Shape defects were restored by complementation.

2 ly observed an indirect mechanism of genetic complementation.

3 ity that could be rescued by embryo-specific complementation.

4 SCs) in mice through interspecies blastocyst complementation.

5 GFP fluorescence by intracellular molecular complementation.

6 g single-EPS-cell-derived mice by tetraploid complementation.

7 normal glycan chain and growth upon genetic complementation.

8 M overlay assay and bimolecular fluorescence complementation.

9 , or in enzymes reconstituted by polypeptide complementation.

10 was detected using bimolecular fluorescence complementation.

11 ce microscopy using Bimolecular Fluorescence Complementation.

12 henotypic alterations were corrected by gene complementation.

13 n organs through the technique of blastocyst complementation.

14 1, which, together with moss-angiosperm gene complementations(6), suggests deep functional conservati

15 nonical lipoate ligases, the enzyme retained complementation activity.

16 lopment of an aptamer-initiated fluorescence complementation (AiFC) method for RNA imaging by enginee

17 Complementation analyses indicated that the biochemical

18 mplex structure and bimolecular fluorescence complementation analyses revealed that the C terminus of

19 Bimolecular fluorescence complementation analyses showed that in stably transform

20 Whereas overexpression and complementation analyses suggest a redundant biochemical

21 Later, complementation analysis confirmed the identity of At3g5

22 idate mutants via competitive infections and complementation analysis in chicks.

23 Complementation analysis of H pylori strains isolated fr

24 in the splicing of bZIP60 mRNA, and through complementation analysis of various mutant forms of IRE1

25 Complementation analysis revealed that replacement of ty

26 Interestingly, bimolecular fluorescence complementation analysis showed that the interaction of

27 Using bimolecular fluorescence complementation analysis we show that, like its sequence

28 in planta based on bimolecular fluorescence complementation and co-immunoprecipitation of the protei

29 linear correlation between catalytic domain complementation and cyclase activity upon stimulation wi

30 I BE from potato, StBE1, resulted in partial complementation and high amylose starch.

31 logy of Toc75 using bimolecular fluorescence complementation and immunogold electron microscopy.

32 We report the partial complementation and subsequent comparative molecular ana

33 tions, including up to 7 per allele, allowed complementation and thus did not block KREPB5 function.

34 IKBKG gene in patients with IP and performed complementation and transactivation assays in NEMO-defic

35 Complementation and transport inhibition experiments rev

36 rom co-immunoprecipitation, protein-fragment complementation, and co-immunofluorescence assays showed

37 ugh fine mapping, genome sequencing, genetic complementation, and gene editing, that haploid inductio

38 tagged receptors, biomolecular fluorescence complementation, and merged confocal images.

39 rom the rDNA was not adequate for functional complementation, and the stalled cells arrested prior to

40 Gaussia princeps luciferase protein-fragment complementation assay (GPCA).

41 ectable levels in a highly sensitive genetic complementation assay and 33 that cause varying reductio

42 seeds in cell culture using protein fragment complementation assay and intracerebral injection of alp

43 We have developed a biosupramolecular complementation assay by assembling a fluorescent Spinac

44 lization of P-TEFb activation by fluorescent complementation assay could be used to find new P-TEFb-r

45 the dihydrofolate reductase protein-fragment complementation assay in Saccharomyces cerevisiae.

46 sing a quantitative bimolecular fluorescence complementation assay in tobacco (Nicotiana benthamiana)

47 Additionally, complementation assay revealed that SlCBL10 is a true or

48 Thus, our genetic complementation assay reveals acute, protein-specific in

49 rmed validation screening with a Split-Rluc8 complementation assay that identified reticulon 1A (RTN1

50 pothesis, we used a retrovirus-based protein complementation assay to find LMW-E binding proteins in

51 Therefore, we optimized a firefly luciferase complementation assay to screen against this chimeric re

52 A biochemical complementation assay was developed to show that a uniqu

53 An Escherichia coli complementation assay was used to validate the results i

54 By applying a split luciferase complementation assay, we identified a functional intera

55 Using a validated bimolecular fluorescence complementation assay, we provide evidence that a UapA o

56 ting EEDs to a TAT-PTD/CPP spilt-GFP peptide complementation assay, we were able to quantitatively me

57 urther validated using NanoLuc-Based Protein Complementation Assay.

58 tion with RIP1 in a bimolecular fluorescence complementation assay.

59 , we used the BiFC (bimolecular fluorescence complementation) assay and report for the first time on

60 of function phenotypes in C. elegans genetic complementation assays and dominant negative activities

61 By both complementation assays and SCRaMbLE (synthetic chromosom

62 Bimolecular fluorescence complementation assays indicate that BKIP-1 interacts wi

63 Split-luciferase complementation assays indicated that Galpha13 in its ac

64 Cross-species complementation assays showed that CeGRS1 effectively re

65 unoprecipitation and bimolecular fluorescent complementation assays showed that MjTTL5 interacts spec

66 Bimolecular complementation assays showed that the TAD of IE62, EHV-

67 ploited a panel of 26 yeast-based functional complementation assays to measure the impact of 179 vari

68 tion of ptch1 in zebrafish for which in vivo complementation assays using these models showed that al

69 ved, phylogenetic analyses and cross-species complementation assays were performed.

70 In complementation assays, the reporter fragments are direc

71 Through mutational analysis and chemical complementation assays, we demonstrate that these polyke

72 o-hybrid system and bimolecular fluorescence complementation assays, we showed that OsHOS1 interacts

73 A-damaging reagents and for lentivirus-based complementation assays, which can be used to systematica

74 causal variants that were validated through complementation assays.

75 co-localization and bimolecular fluorescence complementation assays.

76 down assays, and by bimolecular fluorescence complementation at the apical plasma membrane of pollen

77 ors, binding studies, electrophysiology, and complementation-based assays revealed compounds favoring

78 Here, we used a Bimolecular Fluorescence Complementation-based functional genomics method to perf

79 east homologous recombination, an auxotrophy complementation-based yeast selection system and sequenc

80 69 and Towne strain viruses, consistent with complementation between the UL130 and UL131A expressed b

81 89 and StKRBP1, and bimolecular fluorescence complementation between them, indicate they associate at

82 A biomolecular fluorescent complementation (BiFC) analysis demonstrated that (i) mu

83 Bimolecular fluorescence complementation (BiFC) and co-immunoprecipitation experi

84 of hybrids based on bimolecular fluorescence complementation (BiFC) and found that breast cancer cell

85 through the use of bimolecular fluorescence complementation (BiFC) and super-resolution microscopy.

86 er the last decade, bimolecular fluorescence complementation (BiFC) assay has been widely used to det

87 Here, we describe a bimolecular fluorescence complementation (BiFC) assay to visualize the interactio

88 noprecipitation and bimolecular fluorescence complementation (BiFC) assays in Nicotiana benthamiana l

89 Here we describe a new bifluorescence complementation (BiFC) method that labels a subset of ER

90 dimers, trapped by bimolecular fluorescence complementation (BiFC).

91 , assays relying on bimolecular fluorescence complementation (BiFC; also referred to as split-YFP ass

92 specific and robust bimolecular luminescence complementation (BiLC) reporter system to facilitate the

93 s a firefly luciferase (FLuc) split reporter complementation biosensor (NFLuc-ER-LBDG521T-CFLuc) to s

94 t a molecularly engineered reporter fragment complementation biosensor based on optical imaging of Fi

95 B phenotype was fully rescued by BAC or cDNA complementation but not by the reduction of p53 levels,

96 We propose complementation by codon shuffling as a means to produce

97 In vitro pull-down, bimolecular fluorescence complementation, coimmunoprecipitation, and mass spectro

98 lysis of independent alleles, and transgenic complementation confirm that BLF1 encodes a presumed tra

99 Functional complementation correlates with amino acid sequence iden

100 In principle, complementation could be achieved by placing the reporte

101 ining gene editing with CRISPR/Cas9 and PSCs complementation could result in a powerful approach for

102 ration of a normal tiller angle in fuct-1 by complementation demonstrated that the phenotype is cause

103 Sequential fine mapping and transgenic complementation demonstrated that ZmWAK is the gene with

104 employed, while the reciprocal heterologous complementation demonstrates that U. botrytis MAT genes

105 peptide with serine rendered it incapable of complementation, demonstrating an absolute requirement f

106 ization of the purified protein, and in vivo complementation, demonstrating that there are different

107 ssembly competent state through donor-strand complementation (DSC) and cleft-mediated anchorage.

108 tion and host cell invasion as a readout for complementation efficiency, and identified several Inv/M

109 Interspecies blastocyst complementation enables organ-specific enrichment of xen

110 ed THP, from a DeltamupP strain and chemical complementation experiments confirm that the first step

111 Complementation experiments in Arabidopsis thaliana reve

112 Genetic complementation experiments in conditional separase muta

113 Complementation experiments in mouse embryonic fibroblas

114 ogous cell fusion assay in vitro and allelic complementation experiments in mymk knockdown and mymk(i

115 Complementation experiments in S. cerevisiae provide evi

116 Complementation experiments in yeast lacking glutathione

117 Complementation experiments in yeast showed that the res

118 Furthermore, by complementation experiments in zebrafish, we demonstrate

119 Complementation experiments performed with PstP deletion

120 east two-hybrid and bimolecular fluorescence complementation experiments suggest that GUN1 might tran

121 Functional, mutagenesis and complementation experiments were used to empirically dem

122 Complementation experiments with external application of

123 ry cells (DPY19L1, DPY19L3, and DPY19L4) and complementation experiments with mouse homologs showed t

124 Complementation experiments with SINV suggested that RNA

125 Using an FMDV replicon in complementation experiments, our data demonstrate that t

126 In complementation experiments, the DeltasfaA mutant carryi

127 targeted mutagenesis of cutC and subsequent complementation experiments.

128 we show that partial loss of either APOBEC1 complementation factor (A1CF), the RNA-binding cofactor

129 a viability, we employed "genetic metabolite complementation." First, the trypanosome de novo pathway

130 target proteins, achieving significant FLuc complementation for ERalpha (p < 0.01), p53 (p < 0.005),

131 port an optical assay, based on fluorescence complementation, for visualizing in cellulo APP-BACE-1 i

132 Bimolecular fluorescence complementation, Forster resonance energy transfer, and

133 D51-mediated DNA strand pairing and provides complementation functions exceeding those of BRC repeats

134 on interface of ERCC1 (excision repair cross-complementation group 1) and XPF (xeroderma pigmentosum

135 ed by mutations in the Excision Repair Cross-Complementation group 6 gene (ERCC6).

136 Xeroderma pigmentosum (XP) complementation group A (XPA) is an essential scaffoldin

137 previously measured in Xeroderma pigmentosum complementation group A (XPA) mice that are deficient in

138 Fanconi anemia complementation group C (FANCC) protein interacts with P

139 loss-of-function human haploid cells for FA complementation group C (FANCC), a gene encoding a compo

140 ting the expression of xeroderma pigmentosum complementation group C (XPC) and DNA damage-binding pro

141 pathway by monoubiquitinating Fanconi anemia complementation group D2 (FANCD2) for the initiation of

142 Here, we report that FA complementation group D2 protein (FANCD2) functionally i

143 tion group 1) and XPF (xeroderma pigmentosum complementation group F), leads to severe NER pathway de

144 m this interaction in cells deficient for FA complementation group I and D2 (FANCI and FANCD2) that f

145 ication-blocking lesions than Fanconi anemia complementation group L (FANCL)-null mutants, suggesting

146 to the discovery that Fancl (Fanconi anemia, complementation group L) is downregulated in SALL4B Tg l

147 Genetically, it is assigned to eight complementation groups (XP-A to -G and variant).

148 CS is divided into two primary complementation groups, A and B, with the CSA and CSB pr

149 activating mutations could be divided into 6 complementation groups, resulting in synergistic Rag- an

150 use FA pathologies and have been assigned FA complementation groups.

151 ity of clinical features, between and within complementation groups.

152 helper viruses and reagents that facilitate complementation have enhanced the efficiency of SAD-B19(

153 ese differences, we used luciferase fragment complementation imaging to monitor the recruitment of Cb

154 f PSC-derived islets generated by blastocyst complementation in a xenogeneic host.

155 ct of this mutation was tested by transgenic complementation in the Arabidopsis elf3-1 mutant, with t

156 ich was detected by bimolecular fluorescence complementation in the nucleoplasm and nucleolus.

157 nning the length of delta-COP via functional complementation in yeast, we dissect the domains of the

158 es is an extreme instance of gene expression complementation, in which genes are active in only one o

159 a multivacuolar phenotype, rescuable by gene complementation, indicative of a defect in CCV biogenesi

160 Protein-fragment complementation is a valuable tool for monitoring protei

161 Reversible complementation is desirable, but photodissociation has

162 Although this complementation is typically irreversible, it has been s

163 ssion can be restored to wild-type levels in complementation lines expressing pri-miR163 gene in the

164 regulating lipid and starch metabolism, PSR1 complementation lines in the psr1 strain and PSR1 overex

165 strating the need to use multiple alleles or complementation lines when attributing roles to a gene p

166 , and human disease, interspecies blastocyst complementation might allow human organ generation in an

167 lained by the older duplication-degeneration-complementation model.

168 e investigated the msh1 phenotype using hemi-complementation mutants and transgene-null segregants fr

169 g for subunit IV of the cyt b6f complex, and complementation of a DeltapetD host strain by chloroplas

170 Complementation of a hemO deletion strain with the hemOi

171 ming domains can be identified by functional complementation of a known prion domain.

172 Subcellular localization to the tonoplast, complementation of a manganese (Mn)-sensitive Saccharomy

173 ceptor activation assays based on functional complementation of a split NanoLuc luciferase and used t

174 The complementation of activities between these classes of t

175 bility to the drug was partially restored by complementation of ald.

176 . pylori gene encoding this enzyme, bioV, by complementation of an E. coli bioH deletion strain.

177 otein extraction in vitro and for functional complementation of an msp1 deletion in yeast.

178 Genetic complementation of AVRFOM2 in three different race 2 iso

179 and abortion in pregnant guinea pigs, while complementation of capsule expression almost fully resto

180 logical functions of mitochondria, including complementation of damaged mitochondrial DNAs and the ma

181 95% less oxIAA compared with wild type, and complementation of dao1 restores wild-type oxIAA levels,

182 Complementation of fcpA restored the wild-type morpholog

183 In addition, metabolite analysis and genetic complementation of glycolate transport in yeast showed t

184 pamycin-induced deletion of CRK3 resulted in complementation of growth, whereas expression of an acti

185 Complementation of mouse hepatocytes with hNTCP confers

186 of transvection, a special class of genetic complementation of mutant alleles on homologous chromoso

187 em for plasmid-based transformation enabling complementation of mutations; expression of foreign, mod

188 ptogenetics, membrane sensor technology, and complementation of oxygenic phototrophy.

189 protein (GFP) fusion protein and functional complementation of p12-PM14 occurred in a manner indepen

190 Genetic complementation of patient's cells with wild-type FANCM

191 ng those at conserved DxxxD domains, reduced complementation of pmr1 to different levels.

192 However, complementation of Ren1d(-/-) mice with human renin was

193 n leading to wild-type (WT) reversion during complementation of replication-defective and attenuated

194 Complementation of RM1 with a genomic library revealed t

195 Partial complementation of sec3a resulted in the development of

196 Complementation of the aap mutant with full-length Aap (

197 Complementation of the addAB mutant restored resistance

198 1, one of three EgWRI1 orthologs, by genetic complementation of the Arabidopsis wri1 mutant.

199 Genetic complementation of the disrupted locus with a mutated PI

200 d by genetic deletion from CNB-1 and genetic complementation of the methyl-accepting chemotaxis prote

201 Complementation of the mutant with a wild-type copy of t

202 Complementation of the mutant with the NCgl2760 gene ful

203 NA repair factor Hebo, which is critical for complementation of the patient's DNAdsb repair defect.

204 gene of Kluyveromyces lactis allow for cross-complementation of the respective protein kinase single-

205 Top1p N-terminal domain are required for the complementation of the top1 rDNA phenotypes.

206 micking GUN4(S264D) results in an incomplete complementation of the white gun4-2 null mutant and a ch

207 Complementation of the wild-type race 2 (which lacks Fon

208 Functional complementation of the zrt1/zrt2 yeast mutant by TaZIP3,

209 tation in dendrites involving the controlled complementation of three molecules that simultaneously e

210 ve AHSV1 strain in combination with in trans complementation of VP6.

211 ys with the recombinant proteins or by their complementation of yeast mutants.

212 emperature stress were probed by auxotrophic complementation of yeast with prokaryotic, thermophilic

213 eractions with both CD4 and AP-2 resulted in complementation of YFP and a bright fluorescent signal b

214 When used for plant complementation, one of these mutant variants, GRXS15K83

215 c plant assays have been used frequently for complementation, overexpression or antisense analysis, b

216 NAP tags improve upon other protein fragment complementation (PFC) approaches by offering both multid

217 uantitative live cell split-GFP fluorescence complementation phenotypic assay to systematically analy

218 recombinase enhanced bimolecular luciferase complementation platform (ReBiL).

219 Here, we establish a versatile blastocyst complementation platform based on CRISPR-Cas9-mediated z

220 minated MreB protein interaction and partial complementation provided by CT009 in RodZ deficient Esch

221 ns in specific genes of interest followed by complementation remains problematic for obligates and is

222 is by engineering the monomeric fluorescence complementation reporters, the IkappaBalpha reporter for

223 CPSF6 complementation rescued HIV-1 integration site distribut

224 Bimolecular fluorescence complementation reveals that the adaptor protein 14-3-3,

225 In vivo complementation showed that ClpT function and ClpPR core

226 In vivo complementation shows that both REV7-binding sites in RE

227 LTP, we find that such spatiotemporal signal complementation simultaneously explains three integral f

228 ll ablation approach with a novel blastocyst complementation strategy, we generated murine embryos th

229 different virulence phenotypes using genetic complementation studies and infection assays.

230 Immunohistochemistry and yeast complementation studies demonstrated that the mutations

231 Targeted pocR mutations and complementation studies identified reuterin to be the pr

232 tations in the O-acetyltransferase gene wciG Complementation studies in a wciG deletion strain verifi

233 Complementation studies in yeast (Saccharomyces cerevisi

234 ying a forward genetic approach supported by complementation studies of ecotype Columbia (Col), which

235 Genetic mutagenesis and complementation studies revealed that HP1020 and HP1021,

236 Complementation studies revealed that these enzymes are

237 In vivo complementation studies showed that mutant human NNT fai

238 noprecipitation and bimolecular fluorescence complementation studies showed that the full-length and

239 ares homology with SNF1/AMPKalpha, and yeast complementation studies suggest that it is part of a fun

240 Yeast modeling and complementation studies validated the pathogenicity of t

241 In gene complementation studies, normal human p53 alleles suppre

242 Using mfa5 gene disruption and complementation studies, the authors revealed that Mfa5

243 Our complementation studies, using a mutant lacking the nitr

244 ximations by mutagenesis and residue-residue complementation studies.

245 A deletion mutant and restoration by genetic complementation suggest a nonessential role for this pro

246 ned from Spodoptera frugiperda Using a viral complementation system and RNA interference (RNAi) assay

247 his we have developed a regulatable fragment complementation system for COase cloned from Chromobacte

248 uble or cell-bound HER2, proving this unique complementation system overcomes previous limitations an

249 n2 via the firefly split luciferase fragment complementation system, is genetically encoded in these

250 Using this trans-complementation system, we found that P forms parallel d

251 f-concept of this approach; however, current complementation systems have not met the practical requi

252 plex formation compared to traditional trans-complementation systems.

253 in live cells that utilizes split-luciferase complementation technology based on TREM2 and TYROBP fus

254 Complementation test and gene expression analysis reveal

255 Furthermore, complementation test results suggest that phosphorylatio

256 one of the candidate genes as Rj4 using both complementation tests and CRISPR/Cas9-based gene knockou

257 Complementation tests between spp1 and spp3 revealed the

258 tant was complemented by wild-type At5g32470 Complementation tests in Escherichia coli and enzyme ass

259 Complementation tests in the polyprenol-deficient yeast

260 Allelism and complementation tests revealed that both pf mutants were

261 binding GCAP, remained high, and functional complementation tests showed that the RetGC1 active site

262 Genomic resequencing and complementation tests were used to identify the causal g

263 k2; hence, both in vivo and in vitro genetic complementation tests were used to prove that this Col3a

264 y comparison to published data and by direct complementation tests, finding both dominant and recessi

265 By applying genetic complementation tests, we estimate that about two-thirds

266 mmunoassays, and in the future should enable complementation to be expanded to monitoring endogenous

267 Previously, we used gene disruption and complementation to conclude that the MMAR_0039 gene in M

268 noprecipitation and bimolecular fluorescence complementation to determine that SINA3 specifically int

269 energy transfer and bimolecular fluorescence complementation to establish the dominant functionally i

270 We used bimolecular fluorescence complementation to show that expression of single phosph

271 4 genes only expressed in Mo17 displayed SPE complementation under control and water deficit conditio

272 Heterologous complementation using C. heterostrophus MAT genes shows

273 Full complementation was achieved using the class II BE ZmBE2

274 xpressing transgenic mice, a modest level of complementation was found.

275 noprecipitation and bimolecular fluorescence complementation, we demonstrated the interaction of Rod1

276 unoprecipitation and bimolecular florescence complementation, we found that SsCP1 interacts with PR1

277 ng photoconvertible bimolecular fluorescence complementation, we selectively probe TF2 dimers in the

278 dies, site-directed mutagenesis, and in vivo complementation were used to define the functions of the

279 te distribution in CPSF6 knockout cells, but complementation with a capsid binding mutant of CPSF6 di

280 Complementation with a catalytically dead mutant highlig

281 logy of the mutant could be restored through complementation with a construct encoding a functional W

282 utual gametophytic sterility was overcome by complementation with a genomic construct but not with a

283 Genetic complementation with a plasmid carrying the M.tb H37Rv s

284 rus contamination frequently observed during complementation with an unmodified helper gene.

285 Complementation with codon-shuffled RTA constructs did n

286 Using a combination of genetic complementation with deletion constructs and virus overl

287 icromolar concentrations of putrescine or by complementation with either glycosomal or cytosolic vers

288 , c-di-GMP-induced cell death was rescued by complementation with exogenous DIF-1.

289 homolog (DeltaYggS) is pyridoxine sensitive; complementation with human PROSC restored growth whereas

290 Complementation with MEF8-E549A failed to restore editin

291 ribosylation defect was restored by cellular complementation with normal tRNA[Ser]Sec.

292 Complementation with the HPV-8 E2 hinge motif generated

293 Complementation with the mrm2 allele carrying the equiva

294 und in fibroblasts, which was restored after complementation with wild-type CCDC115.

295 Lentiviral complementation with wild-type MDH2 cDNA restored MDH2 l

296 Complementation with wild-type or acid tolerant F. succi

297 This effect is P27 specific since complementation with wild-type p27 but not p55 fully res

298 Complementation with wild-type, but not mutagenized, C1q

299 The observed defects are rescued by complementation with yeast HEM25 or human SLC25A38 genes

300 both as a soluble cyclase and a reporter of complementation within the catalytic domain.