ライフサイエンスコーパス: concatemer

1 ne of the two genomic ends are formed on the concatemer.

2 g pathway that is initiated by cleavage of a concatemer.

3 sistent with the integration of het DNA as a concatemer.

4 on is introduced to the gamma2L-beta2-alpha1 concatemer.

5 molecular DNA packaging machine on the viral concatemer.

6 ystem for replication and maintenance of DNA concatemers.

7 n hepatocytes containing the double-stranded concatemers.

8 mediate cleavage of genomes from replicative concatemers.

9 zation) of T7 DNA and subsequent cleavage of concatemers.

10 cated product is in the form of long plasmid concatemers.

11 lling circle activity to generate the linear concatemers.

12 nome, arranged mainly as polydisperse linear concatemers.

13 ncatemers; and locus 7 yielded no identified concatemers.

14 ATR in "repair" of E4 mutant genomes to form concatemers.

15 of alpha3beta4alpha5 versus alpha3beta4-only concatemers.

16 plication and ligates the viral genomes into concatemers.

17 NA replication and ligate viral genomes into concatemers.

18 , and joining together of viral genomes into concatemers.

19 lly characterized by the formation of genome concatemers.

20 pports strong FRET in CFP-DsRed or GFP-DsRed concatemers.

21 EGF) expressions were controlled by this HRE concatemer and a minimal simian virus 40 promoter.

22 milar pore properties of the hexameric Orai1 concatemer and native CRAC channels, we conclude that th

23 es are then cut out of a larger, multigenome concatemer and packaged into capsids.

24 echanism, the packaging machinery cuts a DNA concatemer and packages a single unit length genome with

25 osome involves cutting the chromosome from a concatemer and translocating the DNA into a prohead.

26 d that it existed as a high-molecular-weight concatemer and underwent significant levels of homologou

27 wild-type and mutagenized PSA promoter, ARE concatemers and appropriate controls.

28 We find a head-to-tail arrangement in the concatemers and circular permutation in both the monomer

29 Monomeric herpesvirus DNA is cleaved from concatemers and inserted into preformed capsids through

30 rpes simplex virus (HSV) DNA is cleaved from concatemers and packaged into capsids in infected cell n

31 stoichiometry, while studies of linked Orai1 concatemers and single-molecule photobleaching suggest t

32 gene expression (including the formation of concatemers and their subsequent cloning and sequencing)

33 sized linear molecules, head-to-tail genomic concatemers, and complex branched forms with ends at def

34 DNA genomes, in the form of complex branched concatemers, and unstable spherical precursor capsids te

35 2, and 5 produced head-to-head/tail-to-tail concatemers; and locus 7 yielded no identified concateme

36 We have used a pentameric concatemer approach to express defined and consistent po

37 o form several fragments is observed when T7 concatemers are incubated in an extract of T7-infected E

38 In vivo, DNA concatemers are required for packaging.

39 vage of herpesvirus genomes from replicative concatemers are unknown.

40 9-kb band, suggesting a head-to-tail genomic concatemer as the most prominent form in extracted mtDNA

41 and beta2 subunits and establish pentameric concatemers as a means to delineate interactions between

42 bunits increased the rate of inactivation of concatemers, as predicted for subunits that act independ

43 hesis is proposed: the observed packaging of concatemer-associated T7 genomes is cooperative.

44 alpha5, or alpha5DN subunits with a dimeric concatemer (beta2alpha4) in a heterologous system, to ob

45 nown to occur during the packaging of T7 DNA concatemers both in vivo and in vitro.

46 I, are able to produce normal amounts of DNA concatemers but they are not cut, or matured, into unit

47 region, the viral genomic DNA is joined into concatemers by cellular DNA repair factors, and this req

48 lly regardless of which Orai1 subunit in the concatemer carried the mutation.

49 veral technical difficulties associated with concatemer cloning and purification have not been solved

50 d steps, such as purification and cloning of concatemers, colony picking and plasmid DNA purification

51 The packaging substrate is typically a concatemer composed of multiple genomes linked in a head

52 Phage genomes are replicated as linear concatemers composed of multiple copies of the genome jo

53 The RCA product is a concatemer containing tens to hundreds of tandem repeats

54 MutY were found to function processively on concatemers containing 7,8-dihydro-8-oxo-2'-deoxyguanosi

55 nstructed and characterized tetrameric hERG1 concatemers containing a variable number of wild-type su

56 on during replication can produce h-t linear concatemers containing an inversion of single copy seque

57 ed initiators triggered the formation of DNA concatemers containing hemin-binding aptamers through a

58 Poxvirus DNA replication generates linear concatemers containing many copies of the viral genome w

59 as not observed in the reaction of MutY with concatemers containing OG.A mispairs.

60 If the in vivo presence of a similar concatemer-containing DNA network is assumed, requiremen

61 ded by Ad3, Ad7, Ad9, and Ad11, no viral DNA concatemers could be detected.

62 en proposed that the phage packages a linear concatemer created during rolling circle replication of

63 The translocation ATPase and the concatemer-cutting endonuclease reside in terminase.

64 Formation of pac2 ends on concatemers depended on terminal cis sequences, since ec

65 SOAR itself is a dimer, we constructed SOAR concatemer-dimers and introduced mutations at F394, whic

66 complex that mediates both the insertion of concatemer DNA into capsids and its subsequent cleavage

67 tein complex that mediates both insertion of concatemer DNA into capsids and its subsequent cleavage

68 oli and poor in vitro ligation of CTG repeat concatemers due to strand slippage.

69 Mature genomes are cleaved from the concatemer during packaging.

70 from transient head-to-head and tail-to-tail concatemers during replication in the cytoplasm of infec

71 n internal phosphodiester bond and linearize concatemers during rolling circle replication.

72 stent with a model in which pac2 elements at concatemer ends impart a directionality to concatemer pa

73 on is that single genomes are cleaved off of concatemer ends in a preferred direction.

74 binding proteins that initiate insertion of concatemer ends into empty capsids.

75 molecules to generate a head-to-tail linear concatemer, followed by recombination-dependent replicat

76 icating cleavage/packaging of viral DNA from concatemers for packaging into virions, but analyses of

77 Mre11, Rad50, and Nbs1 (MRN) is required for concatemer formation and full activation of damage signa

78 gated the cellular proteins involved in this concatemer formation and how they are inactivated by E4

79 of Nbs1 that are differentially required for concatemer formation and inhibition of Ad DNA replicatio

80 12 also failed to complement defects in both concatemer formation and late protein production of a vi

81 Concatemer formation can be prevented by the E4orf3 prot

82 Concatemer formation is dependent upon the cellular Mre1

83 plication in the absence of E4 is not due to concatemer formation or DNA damage signaling.

84 individual contributions of the MRN complex, concatemer formation, and damage signaling to viral DNA

85 host nucleus, resulting in circularization, concatemer formation, or chromosomal integration.

86 ation was a critical event in the process of concatemer formation.

87 B-55K to degrade MRE11, preventing viral DNA concatemer formation.

88 junctions between genomes within replicative concatemers formed late in infection almost exclusively

89 ytes showed double-stranded and head-to-tail concatemer forms but failed to show integration of the A

90 The results revealed that amplicon concatemers frequently contain adjacent amplicon units w

91 d recombination may result in viral-host DNA concatemers, frequently disrupting genes involved in onc

92 Mouse fibroblasts transfected with the concatemers gave a CAT activity that was 14-fold greater

93 1) excision of a unit length genome from the concatemer (genome maturation) and 2) translocation of t

94 n in equilibrium between circular and linear concatemer genomes caused by the lack of DNA-PKcs activi

95 ed rAAV molecules into high-molecular-weight concatemers in about 5% of hepatocytes.

96 o formation and accumulation of head-to-tail concatemers, in addition to the usual head-to-head and t

97 the liver was approximately 0.2, suggesting concatemer integration.

98 (6A(4)) or SP-A2(1A(3)) were integrated as a concatemer into the genome of each of the two hTG lines.

99 e copies of the transgene can integrate as a concatemer into the sperm genome, and more than one site

100 ay junction resolvase is required to process concatemers into unit-length genomes for packaging.

101 Processing of viral DNA concatemers into unit-length genomes was unimpaired at e

102 ired for the in vivo resolution of viral DNA concatemers into unit-length genomes with hairpin telome

103 ) is defective in both cleavage of viral DNA concatemers into unit-length monomers and packaging of v

104 aves the concatemerized DNA within this 8 nt concatemer junction (CJ).

105 near promoters and near the right end of the concatemer junction almost certainly must relate to lyso

106 DNA and at a site near the right end of the concatemer junction of T7 DNA.

107 T/A), well conserved at the right-end of the concatemer junction of T7-like phages.

108 aining the entire VAC genome, with an intact concatemer junction sequence, were identified.

109 ion and in pausing and termination at the T7 concatemer junction.

110 85) reduce pausing and termination at the T7 concatemer junction.

111 eaves DNA four-way junctions extruded at the concatemer junctions to produce monomeric genomes.

112 -field gel electrophoresis, (iv) cleavage of concatemer junctions was inhibited, and (v) virion morph

113 mechanism, we used a series of V. riparia K2 concatemers (K4, K6, K8, and K10) and natural dehydrins

114 n and therefore were not part of a canonical concatemer made by replication.

115 wed that packaged phage had not been part of concatemers made by recombination or by annealing at cos

116 suggesting that both circular and linear AAV concatemers may have contributed to the trans-splicing-m

117 factor-1alpha during hypoxia and features a concatemer of four XRE cores (GCGTG).

118 A concatemer of nine copies of the consensus sequence of H

119 Integration into a 32-unit concatemer of target DNA was markedly more efficient tha

120 This is the first time that a concatemer of the complete pentameric receptor has been

121 A concatemer of the cpr-1 -147 GATA motif placed upstream

122 nant allele, V99A(T)/V99A(T), a head-to-tail concatemer of three V99A targeting constructs.

123 ents lie near the ends formed on replicative concatemers of four herpesviruses: herpes simplex virus

124 rain stoichiometry, fusion proteins encoding concatemers of human alpha3, beta4, and alpha5 (D and N

125 hannel stoichiometry by expressing hexameric concatemers of human Orai1 and comparing in detail their

126 ese models, we have designed and synthesized concatemers of Rev-binding elements (RBEs) that fold to

127 hat the presence of two ER export motifs (in concatemers of SERT and GABA transporter-1) supported re

128 By using concatemers of subunits and chimeric subunits, we have f

129 We generated concatemers of subunits so that the alpha1 subunits coul

130 ce found at the junction of the head-to-tail concatemers of T7 genomic DNA generated during T7 DNA re

131 ealing (SSA) reaction to generate end-to-end concatemers of the phage genome for packaging.

132 n defined by the primers but also continuous concatemers of the template.

133 adenovirus E4 mutants accumulate end-to-end concatemers of the viral genome that are assembled from

134 Multiple copy transformants often integrated concatemers of transforming DNA.

135 was based on circularization of head-to-tail concatemers of VAC DNA.

136 te for packaging is a head-to-tail multimer (concatemer) of the mature 40-kilobase pair genome.

137 A CanScript concatemer offered enhanced activity.

138 Human RK (hRK) promoter and its concatemers or derivatives extending into the conserved

139 ment formed at the junction of HHV-6B genome concatemers (pac2-pac1) is necessary and sufficient for

140 t concatemer ends impart a directionality to concatemer packaging by binding proteins that initiate i

141 mical evidence of a requirement for gp55 for concatemer packaging to assemble active wild-type phage

142 ation intermediates the loci produced, while concatemer processing gave rise to the circularized DNAs

143 donucleolytic cleavage of immature viral DNA concatemer recognized by TerS, assembles into a pentamer

144 us genome mediated formation of pac2 ends on concatemers regardless of the orientation of their inser

145 ion and maintenance in Escherichia coli; DNA concatemer resolution was inhibited leading to formation

146 and roles in DNA replication, recombination, concatemer resolution, and transcription were suggested.

147 ry high frequency: up to 60% of the amplicon concatemers retrieved from virion particles underwent in

148 nisms in the same or alternate subunits of a concatemer revealed that both intra- and intersubunit co

149 The observed in vitro packaging of a concatemer's genomes always occurs in a synchronized clu

150 Our data suggest that this XRE concatemer site concurrently regulates the expression of

151 ADsubTRS1 exhibited normal cleavage of DNA concatemers, so the defect in C-capsid production must o

152 s with Y- or X-structures in the replicative concatemer substrate by employing a portal-bound Hollida

153 e used a linear multi-abasic site substrate (concatemer), synthesized by ligating together identical

154 ontinuing around the other end to generate a concatemer that is subsequently resolved into unit genom

155 Herpesvirus genomic DNA is cleaved from concatemers that accumulate in infected cell nuclei.

156 However, whereas DNA concatemers that accumulate in prohead and terminase def

157 The latter generates concatemers that are cleaved and packaged into infectiou

158 es replicate their linear genomes by forming concatemers that must be resolved into monomeric units t

159 minase introduces staggered nicks to cut the concatemer to generate unit-length virion chromosomes.

160 nylated proteins indicated that breakdown of concatemers to individual subunits was minimal.

161 nded DNA genome of adenovirus is joined into concatemers too large to be packaged.

162 Using the multi-abasic site concatemer, we demonstrated that AP endo was capable of

163 ual head-to-head and tail-to-tail forms; the concatemers were circularized by homologous or Cre-loxP-

164 Concatemers were expressed in Xenopus laevis oocytes, an

165 These unusual concatemers were generated through homologous recombinat

166 These concatemers were identical to signaling microclusters, a

167 ate gene expression and resolution of genome concatemers were not detected.

168 Fourth, concatemers were partially digested with NlaIII before c

169 mined that the 25-mer monomer from which the concatemers were prepared was nicked by AP endo in a fas

170 A new model is proposed in which concatemers were separated into single units by a "snap-

171 umulate high-molecular-weight linear plasmid concatemers when transformed with plasmids carrying the

172 ges and herpes viruses replicate genome as a concatemer which is cut by a 'headful' nuclease upon com

173 tranded DNA genomes as high-molecular-weight concatemers which are subsequently cleaved into unit-len

174 y ligation of the digestion products yielded concatemers which migrated as a single band in agarose g

175 the host genome, forming mainly head-to-tail concatemers with occasional deletions of the inverted te

176 hage T4-like networks of highly branched mp1 concatemers with up to 20 monomer units were mapped and

177 near genomic monomers and head-to-tail (h-t) concatemers within inverted repeat sequences (IRs) near

178 e event begins with a single repeat within a concatemer yet produces two repeats, one at each of the

179 is that RCR should generate tandem Helitron concatemers, yet almost all Helitrons identified to date