ライフサイエンスコーパス: concatemeric

1 Using a concatemeric [(32)P]U.A DNA polynucleotide substrate to

2 pharmacological properties matching those of concatemeric alpha3beta4* nAChRs.

3 sed lentiviral vectors and developed a novel concatemeric array transfection technique for the introd

4 first steps of poxvirus DNA synthesis yield concatemeric arrays of covalently linked genomes.

5 Von Willebrand factor, an ultralarge concatemeric blood protein, must bind to platelet GPIbal

6 Functional analysis of concatemeric channels, which permit mutagenic manipulati

7 activation by using wild type and engineered concatemeric channels.

8 was packaged into infectious particles in a concatemeric configuration.

9 ta2gamma2L channels, selectively into either concatemeric construct altered the mode of activity elic

10 amma2L-beta2-alpha1 and beta2-alpha1 subunit concatemeric constructs expressed in human embryonic kid

11 iation by neurosteroids, into one of the two concatemeric constructs had a relatively small effect on

12 We used beta2alpha1gamma2L and beta2alpha1 concatemeric constructs to determine the functional effe

13 question using alpha4 and beta2 subunits in concatemeric constructs with the alpha5 subunit, express

14 2delta receptors employing free subunits and concatemeric constructs, expressed in Xenopus oocytes, H

15 rolled by a synthetic promoter consisting of concatemeric copies of the cis-acting site recognized by

16 nslocation and a nuclease activity that cuts concatemeric DNA and generates the termini of viral geno

17 infection and are re-formed by cleavage from concatemeric DNA are unknown.

18 in which BDCRB permits limited packaging of concatemeric DNA but induces skipping of cleavage sites.

19 on that give rise to persistent circular and concatemeric DNA episomes through intramolecular and int

20 nitiation of phage T4 packaging on "endless" concatemeric DNA in vivo by terminase depends upon inter

21 Although cleavage of concatemeric DNA intermediates to unit-length genomes re

22 mplex (terminase) that is presumed to cleave concatemeric DNA into genome lengths.

23 h containing a nuclease domain that resolves concatemeric DNA into genome-length units.

24 results from an ATP-driven translocation of concatemeric DNA into the prohead by the phage terminase

25 s a double-stranded DNA virus that processes concatemeric DNA into virion chromosomes by cutting at s

26 cteriophage lambda, is crucial for packaging concatemeric DNA into virions.

27 y tailed bacteriophages and herpesviruses, a concatemeric DNA is cut and inserted into an empty proca

28 n attractive drug target because cleavage of concatemeric DNA is not required in mammalian cell DNA r

29 onment within an Escherichia coli cell, (ii) concatemeric DNA is required for the successful completi

30 ome maturation is a complex process in which concatemeric DNA molecules are translocated into capsids

31 luorescence microscopy is used to observe T7 concatemeric DNA packaging at the level of a single (mic

32 lex that excises a unit length genome from a concatemeric DNA precursor (genome maturation) and conco

33 da is the excision of a single genome from a concatemeric DNA precursor and insertion of genomic DNA

34 , tails, and genomes that are excised from a concatemeric DNA precursor.

35 ase II alpha also is required for untangling concatemeric DNA progeny for optimal transcription of la

36 licate on noncomplementing cells but cleaved concatemeric DNA to ca. 35 to 98% of wild-type levels.

37 lease domain (amino acids 361-610) that cuts concatemeric DNA to generate a headful-size viral genome

38 with this complex process is the cutting of concatemeric DNA to initiate and terminate DNA packaging

39 ambda, introduces staggered nicks into viral concatemeric DNA to initiate genome packaging.

40 infected with these mutants, indicating that concatemeric DNA was cleaved efficiently.

41 10% wild-type efficiency, 55am33am defective concatemeric DNA was packaged at least 100-fold less eff

42 ow the accumulation of human cytomegalovirus concatemeric DNA while the formation of new genomes was

43 ed catalysis mechanism for cleavage of viral concatemeric DNA.

44 al protein of proheads and cuts and packages concatemeric DNA.

45 t generates mature chromosomes from immature concatemeric DNA.

46 thesized viral DNA remained in a branched or concatemeric form that caused it to be trapped at the ap

47 ocation and an endonuclease that cleaves the concatemeric genome at both initiation and completion of

48 an cytomegalovirus terminase complex cleaves concatemeric genomic DNA into unit lengths during genome

49 We show here that polygenomic concatemeric HCMV DNA does not mature to unit genome len

50 for the cleavage and packaging of replicated concatemeric herpes simplex virus type 1 (HSV-1) DNA cor

51 s the dimer interface and the behaviour of a concatemeric human homologue argue that the transport cy

52 ain portions of the HDR clones contained the concatemeric IDLV genomic structure at the target site,

53 ormed by site-specific cleavage from complex concatemeric intermediates.

54 of loci 3, 4, 6, and 8 produced head-to-tail concatemeric intermediates; loci 1, 2, and 5 produced he

55 n lambda virions are generated by nicking of concatemeric intracellular DNA by terminase, the lambda

56 ct on the functional activity of the minimal concatemeric junction (pac2-pac1).

57 V genome and to the same sequence within the concatemeric junction of replication intermediates.

58 The concatemeric junction sequence also allowed for the pack

59 Experiments revealed that the concatemeric junction sequence possesses an unusual, S1

60 The sequences of concatemeric junctions of viral DNAs were determined, wh

61 predict equimolar populations of h-t linear concatemeric molecules differing only in the relative or

62 on of cre must have contributed to resolving concatemeric molecules either prior to or after DNA inte

63 Linkage of concatemeric monomers was defined at a nucleotide level,

64 We used a fully linked subunit concatemeric nAChR approach to express pure populations

65 ch enzyme was incubated with double-stranded concatemeric polynucleotides containing a site-specific

66 The enzymes excise a single genome from a concatemeric precursor (genome maturation) and then pack

67 This mutant was able to cleave the concatemeric products of viral DNA replication into mono

68 Concatemeric receptors activated by GABA exhibited the s

69 her GABA concentrations, suggesting that the concatemeric receptors have a lower affinity to GABA.

70 Pharmacological tests demonstrated that the concatemeric receptors were potentiated by pentobarbital

71 Herpesvirus DNA replication proceeds via concatemeric replicative intermediates that are comprise

72 Herpesvirus DNA replication proceeds via concatemeric replicative intermediates that are comprise

73 rcles by a rolling mechanism, producing long concatemeric RNAs (approximately 7,500 nt).

74 ith currents from receptors composed of five concatemeric subunits in which the subunit stoichiometry

75 ransmitter GABA and basal activity employing concatemeric ternary GABAA receptors expressed in Xenopu

76 re known to mediate cutting and packaging of concatemeric vegetative DNA.

77 ral terminase are required for processing of concatemeric viral DNA and packaging of individual viral

78 ns a large subunit that is thought to cleave concatemeric viral DNA during the packaging initiation a

79 uses use powerful molecular motors to cleave concatemeric viral DNA into genome-length units and pack

80 e 1 U(L)28 gene is essential for cleavage of concatemeric viral DNA into genome-length units and pack

81 s required to release monomeric genomes from concatemeric viral DNA.

82 required to release individual genomes from concatemeric viral DNA.IMPORTANCE This paper shows a rol

83 of translocation of an intricately branched concatemeric viral genome.