1 r proteins (e.g., helicases, chelatases, and
copines).
2 ng properties similar to those of Paramecium
copine.
3 7, a secretory vesicle-binding protein, as a
copine.
4 in-protein interaction for the VWA domain of
copines.
5 of unknown function were identified, such as
Copine 1 and PICOT, whose developmental regulation was p
6 Mutation of the Arabidopsis CPN1 (
COPINE 1) gene causes a humidity-sensitive lesion mimic
7 volved in signalling, such as importin 7 and
copine 1, cytoplasmic intermediate filament (IF) protein
8 However, the
copine amino acid sequences lack the traditional kinase
9 The highest levels of
copine are found in the spleen.
10 The
copines are a newly identified class of calcium-dependen
11 The
copines are a novel group of Ca(2+)-dependent, phospholi
12 The
copines are a widely distributed class of calcium-depend
13 Copines are calcium-dependent membrane-binding proteins
14 Copines are calcium-responsive, phospholipid-binding pro
15 athway for calcium signaling because we find
copines are capable of interacting with a wide variety o
16 Copines are highly conserved proteins with lipid-binding
17 The biological roles of most
copines are not understood and the biochemical propertie
18 However, the biological functions of the
copines are unknown.
19 A consensus sequence for the coiled-coil
copine-
binding site was derived and found to have predic
20 In the majority of cases the
copine binds to a domain of the target protein that is p
21 The full-length sequences reveal that
copines consist of two C2 domains at the N terminus foll
22 rties and distribution of a native mammalian
copine,
copine I.
23 dicted transmembrane domain and a C-terminal
copine domain and binds to the M-line/dense body protein
24 The
copine domain of RGLG2 exhibited the strongest interacti
25 Antibodies specific to
copine domain protein atypical-1 (CPNA-1), as well as a
26 Thus, the
copine family in Arabidopsis may have effects in promoti
27 gem-4 encodes a member of the
copine family of Ca(2+)-dependent phosphatidylserine bin
28 The
copines,
first described by Creutz et al., comprise a tw
29 The Arabidopsis
copine gene BON1/CPN1 is a negative regulator of cell de
30 The Arabidopsis
copine gene BON1/CPN1 was previously shown to negatively
31 It belongs to the
copine gene family, which is conserved from protozoa to
32 mutant combinations we show that these three
copine genes have overlapping functions essential for th
33 as in other organisms, there is a family of
copine genes, BON1, 2 and 3.
34 mecium was found to have two closely related
copine genes, CPN1 and CPN2.
35 The major protein obtained, named
copine,
had a mass of 55 kDa, bound phosphatidylserine b
36 data bases indicate the presence of multiple
copine homologs in green plants, nematodes, and humans.
37 ally competed by Ca(2+), suggesting that the
copine I A domain may be a functional MIDAS metal bindin
38 Copine I exhibits Mn(2+) binding activity that is strong
39 antiserum raised against a fragment of human
copine I was used to identify chromobindin 17, a secreto
40 A human homolog,
copine I, was expressed in bacteria as a fusion protein
41 d distribution of a native mammalian copine,
copine I.
42 Copine-
I abolishes NF-kappaB transcription by inducing e
43 Knockdown of
copine-
I by siRNA increases tumor necrosis factor alpha-
44 ha-stimulated NF-kappaB transcription, while
copine-
I expression blocks endogenous transcription.
45 Our work provides evidence that
copine-
I regulates the half-life of NF-kappaB transcript
46 Copine-
I stimulates endoproteolysis of p65 within a cons
47 In this study we identify
copine-
I, a calcium phospholipid-binding protein, as a n
48 had correspondingly high kinase activity in
copine III antiserum immunoprecipitate.
49 ified endogenous copine III, and recombinant
copine III expressed in Saccharomyces cerevisiae.
50 ted in all in vitro kinase assays containing
copine III immunoprecipitate or purified copine III.
51 Therefore, the data suggest
copine III may possess an intrinsic kinase activity and
52 no acid analysis revealed phosphorylation of
copine III on serine and threonine residues.
53 Cell lines expressing high levels of
copine III protein had correspondingly high kinase activ
54 The 5 kb
copine III transcript is expressed ubiquitously as deter
55 pine III, chromatography-purified endogenous
copine III, and recombinant copine III expressed in Sacc
56 performed with immunoprecipitated endogenous
copine III, chromatography-purified endogenous copine II
57 ing copine III immunoprecipitate or purified
copine III.
58 fy, partially microsequence, and clone human
copine III.
59 Roles for
copine in binding membranes and target proteins or small
60 Purified native
copine is a 58 kDa monomer that exhibits Ca(2+) self-ass
61 f a 920-base pair partial cDNA revealed that
copine is a novel protein that contains a C2 domain like
62 We also show that interaction with
copines may result in recruitment of target proteins to
63 We provide evidence that
copines,
members of a ubiquitous family of calcium-depen
64 Here, we describe a humidity-sensitive
copine mutant in Arabidopsis.
65 lysis reveals an unanticipated regulation of
copine protein localization and function by calcium and
66 The
copine target proteins were identified by yeast two-hybr
67 to have predictive value for identifying new
copine targets.
68 A protocol for purifying
copine to homogeneity from bovine spleen is described.
69 ory vesicles, as well the general ability of
copines to bind phospholipid bilayers in a calcium-depen
70 f a CRH-1/CREB transcriptional target (gem-4
Copine),
which parallels the effects of human Shank copy
71 No enzymatic function has been attributed to
copines yet.