ライフサイエンスコーパス: coralyne

1 bound, c-di-AMP enhances the fluorescence of coralyne.

2 f a complex of 20-repeat adenosine (A20) and coralyne.

3 r in bacteria) did not form any complex with coralyne.

4 exhibit strong electrostatic attraction with coralyne.

5 use of the triplex-stabilizing intercalator coralyne.

6 nted with the triplex selective intercalator coralyne.

7 ence of the triplex-stabilizing intercalator coralyne.

8 Under optimal conditions (5 muM coralyne, 1 muM poly A20, and 10mM HEPES), this probe ex

9 by adding the triplex selective intercalator coralyne (1000-fold stabilization).

10 We recently demonstrated that coralyne, a small crescent-shaped molecule, can cause th

11 lyne molecules complexed with A20 through A2-coralyne-A2 coordination.

12 a large change in the absorption spectrum of coralyne and also a substantial fluorescence quenching t

13 dine (a dual poison of both topos I and II), coralyne and its derivatives have marginal poisoning act

14 Protoberberine alkaloids (coralyne and its derivatives), which exhibit antileukemi

15 It seems possible to develop coralyne and nitidine derivatives as new topo I-targeted

16 /T triplex was unaffected by the presence of coralyne and was only enhanced 1.4-2.8-fold when the TFO

17 zimidazoles, indolocarbazoles, nitidine, and coralyne) and various types of DNA lesions (e.g., UV dim

18 r optimal conditions (10 nM A8-MB-A8, 800 nM coralyne, and 0.5 mM Ca(2+) ions), the proposed system c

19 Putative triplex (BePI, coralyne, and berberine) and tetraplex [H(2)TmPyP, 5,10,

20 10(7) M(-1)), and that the crescent shape of coralyne appears necessary for poly(A) binding.

21 of a (dA)16*(dT)16 sample in the presence of coralyne at room temperature contains three different se

22 -studied process of triplex stabilization by coralyne binding is found to be a length-dependent pheno

23 rescence of unbound coralyne but not that of coralyne bound to c-di-AMP.

24 lectively quench the fluorescence of unbound coralyne but not that of coralyne bound to c-di-AMP.

25 polyadenine, which forms a 2:1 complex with coralyne, c-di-AMP forms a higher order complex with cor

26 We report that coralyne can cause the complete and irreversible disprop

27 That is, coralyne causes the strands of duplex poly(dT)*poly(dA)

28 ing propidium iodide, Hoechst dye 33258, and coralyne chloride did not inhibit CpG-ODN effect, nor di

29 d coralyne dimerization, the fluorescence of coralyne decreased as a function of the concentration of

30 n to be specifically sensitive to killing by coralyne derivatives and nitidine, suggesting that cellu

31 ant topo I, are only marginally resistant to coralyne derivatives and nitidine.

32 Because heparin promoted coralyne dimerization, the fluorescence of coralyne decr

33 Coralyne-disproportioned (dA)32.

34 show that the kinetics and thermodynamics of coralyne-driven duplex disproportionation strongly depen

35 Coralyne emits relatively weak fluorescence in an aqueou

36 his rule and that it can form complexes with coralyne, even at low micromolar concentrations.

37 the topoisomerase I inhibitors nitidine and coralyne exhibited quite different effects on the same l

38 y has shown that poly(dA) in the presence of coralyne forms an anti-parallel duplex, however attempts

39 the presence of Ca(2+) ions, OSCS can remove coralyne from the MB stem, initiating fluorescence of th

40 , c-di-AMP forms a higher order complex with coralyne (>/=6:1).

41 DNase I footprinting demonstrated that coralyne has a moderate preference for triplexes over du

42 presence of heparin and the formation of the coralyne-heparin complex caused coralyne to be removed f

43 as a duplex for months after the addition of coralyne, if the sample is maintained at 4 degrees C.

44 ronger than the coordination between A20 and coralyne in a 4-(2-hydroxyethyl)-1-piperazineethanesulfo

45 plex, triplex or (dA)n self-structure] binds coralyne in a length-dependent manner.

46 osine-repeated molecular beacon (MB) stem to coralyne in the presence of Ca(2+) ions.

47 trongly suggest the involvement of N7 in the coralyne-induced A.A base pairs.

48 se substitution experiments predict that the coralyne-induced homo-(dA) duplex structure adopts the t

49 icantly reduced the thermal stability of the coralyne-induced homo-(dA) structure.

50 tional theory calculations-revealed that the coralyne-induced spatial asymmetry in the electron state

51 duplex disproportionation are stabilized by coralyne intercalation.

52 homo-(dA) anti-parallel duplexes and docked coralyne into the six most favorable duplex structures.

53 e-specific intercalation of small molecules (coralyne) into a custom-designed 11-base-pair DNA duplex

54 Coralyne is a small crescent-shaped molecule known to in

55 Coralyne is an alkaloid drug that binds homo-adenine DNA

56 Coralyne is known to form strong complexes with polyaden

57 We propose that duplex disproportionation by coralyne is promoted by both the triplex and the poly(dA

58 electrostatic attraction between heparin and coralyne is substantially stronger than the coordination

59 Measured current-voltage curves of the DNA-coralyne molecular junction show unexpectedly large rect

60 In the presence of A20, coralyne molecules complexed with A20 through A2-coralyn

61 oly(dA) self-structure intercalation is 0.25 coralyne molecules per adenine base.

62 ored by as little as one molar equivalent of coralyne per eight base pairs of initial duplex.

63 The presence of coralyne promotes these A-A mismatches to form a hairpin

64 luorescence of coralyne was observed because coralyne remained separate from water in the hydrophobic

65 A titration of poly(dT)*poly(dA) with coralyne reveals that disproportionation is favored by a

66 isproportionation of duplex (dA)16*(dT)16 by coralyne reverts over the course of hours if the sample

67 self-structure having binding constants for coralyne that are greater than that of duplex poly(dT)*p

68 We demonstrate that coralyne, the first small molecule discovered to bind po

69 iological buffer supplemented with 40 microM coralyne, the G/A triplex (Ka = 3.0 x 10(8) M-1) was mor

70 ation of the coralyne-heparin complex caused coralyne to be removed from the A20-corlayne complex.

71 Coralyne was found to bind strongly to single-stranded p

72 An increase in the fluorescence of coralyne was observed because coralyne remained separate

73 order to analyse the sequence preferences of coralyne we have used a combination of DNase I footprint

74 reas polyadenine reduces the fluorescence of coralyne when bound, c-di-AMP enhances the fluorescence

75 alating drugs have been described, including coralyne, which preferentially binds triple helices, tho

76 A) forms a self-structure in the presence of coralyne with a melting temperature of 47 degrees C, for

77 This poly(dA) self-structure binds coralyne with an affinity that is comparable with that o