ライフサイエンスコーパス: coronin

1 an ezrin-radixin-moesin-family protein, and coronin.

2 but no genetic interaction with deletion of coronin.

3 e generated coronin 1-/- mice and found that coronin 1 exerted an inhibitory effect on cellular stead

4 ive role of the WD repeat containing protein coronin 1 in the maintenance of naive T cells in periphe

5 We show that coronin 1 is dispensable for thymocyte survival and deve

6 Whereas coronin 1 was required for chemokine-mediated migration,

7 We generated coronin 1-/- mice and found that coronin 1 exerted an in

8 Importantly, coronin 1-deficient mice possessed comparable levels of

9 est the existence of a hitherto unrecognized coronin 1-dependent decision switch early during life th

10 cell survival was found to be independent of coronin 1.

11 cruitment of a new effector protein known as Coronin-1 (also called Coro1a).

12 In contrast, the presence of Coronin-1 (wild-type neurons) suppresses but does not ha

13 These results establish Coronin-1 as an essential component of a feedback loop t

14 at periods before and after NGF-TrkA-induced Coronin-1 expression (and likely other factors) defines

15 ogether with previous work demonstrating the Coronin-1 expression is NGF dependent, this work suggest

16 Beyond influencing endosomal trafficking, Coronin-1 is also required for several NGF-TrkA-dependen

17 ysosomes sixfold to tenfold faster than when Coronin-1 is intact.

18 In the absence of Coronin-1 we find that NGF-TrkA-PI3K signaling drives ro

19 e the role of the NGF-TrkA effector protein, Coronin-1, on postganglionic sympathetic neuron final ta

20 In the absence of Coronin-1, the NGF-TrkA signaling endosome fuses to lyso

21 ppression in axon growth behaviors is due to Coronin-1-dependent calcium release via PLC-gamma1 signa

22 We also define a new Coronin-1-dependent trafficking event in which signaling

23 Here, we identify coronin 1A (Coro1A) as a novel regulator of beta2 integr

24 Coronin 1A (Coro1A) is involved in cytoskeletal and sign

25 Coronin 1A (Coro1A) is the hematopoietic-specific member

26 lack this posttranslational modification of coronin 1a and exhibit defective TCR-induced actin polar

27 Thus, the phenotype of T cell-specific Coronin 1a deletion resembles the phenotype observed wit

28 subsets were not affected by the deletion of Coronin 1a Furthermore, T cell-specific Coronin 1a knock

29 Our findings establish a function for coronin 1A in T cell egress, identify a surface of coron

30 Nevertheless, because Coronin 1A is strongly expressed in all leukocyte subset

31 Studies analyzing complete Coronin 1a knock-out mice have shown that this molecule

32 n of Coronin 1a Furthermore, T cell-specific Coronin 1a knock-out mice were largely resistant to the

33 type observed with conventional (whole body) Coronin 1a knock-out mice.

34 uestion, we have generated a T cell-specific Coronin 1a knock-out mouse (Coro1a(fl/fl) x Cd4[Cre]).

35 The discovery of another coronin 1A mutant during an N-ethyl-N-nitrosourea-mutage

36 iated phosphorylation of the actin modulator coronin 1a on threonine 418.

37 Deletion of Coronin 1A specifically in T cells led to a strong reduc

38 c acid at position 26 in the actin regulator coronin 1A that enhanced its inhibition of the actin reg

39 he negative regulator of F-actin generation, Coronin 1A, at the center of the T cell interface with t

40 of the predominant T cell-intrinsic role of Coronin 1A.

41 ared with Cd4[Cre] mice expressing wild-type Coronin 1A.

42 attributed to a T cell-intrinsic function of Coronin 1A.

43 rited missense mutation in the gene encoding Coronin-1A (CORO1A) in the 3 siblings.

44 Coronin-1A (CORO1A) is a regulator of actin dynamics imp

45 Coronin-1A (Coro1A) was identified as a differentially e

46 ree-dimensional reconstruction, we show that coronin-1A binds to three protomers in F-actin simultane

47 In this issue of Immunity, the Coronin-1A gene Coro1a, which regulates cytoskeletal str

48 mutation of the filamentous actin-inhibiting Coronin-1A gene.

49 Our results demonstrate that Coronin-1A is required for the development of systemic l

50 tion in patients associated with hypomorphic Coronin-1A mutation.

51 ctin may participate in the interaction with coronin-1A.

52 Coronin 1B (Coro1B) and 1C (Coro1C) were both found to b

53 Coronin 1B also directs SSH1L to lamellipodia where SSH1

54 Coronin 1b and Rab13 proteins were expressed in cultured

55 Finally, Coronin 1b and Rab13 were induced in the injured spinal

56 xperiments show that the interaction between Coronin 1B and the Arp2/3 complex is regulated by protei

57 esis, we have identified serine 2 (Ser-2) on Coronin 1B as the major residue phosphorylated by PKC in

58 hibition is attenuated by phosphorylation of Coronin 1B at Serine 2, a site targeted by SSH1L.

59 e assay to identify cells with an absence of Coronin 1B brought about by the successful infection of

60 We report that Coronin 1B disassembles Arp2/3-containing actin filament

61 Accordingly, depleting Coronin 1B increases phospho-Cofilin levels, and alters

62 Coronin 1B inhibits filament nucleation by Arp2/3 comple

63 We report that Coronin 1B interacts simultaneously with Arp2/3 complex

64 Our results show that Coronin 1B is a ubiquitously expressed member of the mam

65 go apoptosis within an epithelial monolayer, coronin 1B is also recruited to the junctional cortex at

66 Coronin 1B is phosphorylated by PKC both in vitro and in

67 In the absence of coronin 1B or cofilin, Tpm1.8/9 protein levels are reduc

68 Moreover, RNAi gene silencing for Coronin 1b or Rab13 in NGF-treated PC-12 cells markedly

69 Furthermore, Coronin 1B phosphorylation is responsible for a signific

70 These data demonstrate that Coronin 1B regulates leading edge dynamics and cell moti

71 We conclude that Coronin 1B replaces the Arp2/3 complex at actin filament

72 Rat2 fibroblasts expressing the Coronin 1B S2A mutant show enhanced ruffling in response

73 Cells expressing the Coronin 1B S2D mutant have attenuated PMA-induced ruffli

74 Coronin 1B targets actin branches in a manner that is mu

75 hanges could be rescued by overexpression of Coronin 1B together with a constitutively active Cofilin

76 y, clonal cell lines with siRNA knockdown of Coronin 1B were generated using the arrays and compared

77 We conclude that Coronin 1B's coordination of filament formation by Arp2/

78 preferentially interacts with phosphorylated Coronin 1B, allowing it to complex with Slingshot phosph

79 conclude that WISp39 associates with Hsp90, Coronin 1B, and SSH to regulate Cofilin activation and A

80 WISp39 binds Coronin 1B, known to regulate the Arp2/3 complex and Cof

81 small GTPase Rab13 and actin-binding protein Coronin 1b, showed significantly increased mRNA expressi

82 cks the actin-Arp2/3 interaction [4, 5], and coronin 1B, which acts by directing SSH1L to the lamelli

83 Reorganization requires coronin 1B, which is recruited to junctions by E-cadheri

84 , a phenotype consistent with cortactin- and coronin 1B-deficient cells [2, 7].

85 n combined with actin disassembly co-factors Coronin-1B and actin-interacting protein 1 (AIP1), and t

86 s the inhibitory effect of nonphosphorylated coronin-1b on actin nucleation.

87 In addition, METH-induced coronin-1b phosphorylation diminishes the inhibitory eff

88 We discover that both coronin-1C and caveolin retrieve Rac1 from similar locat

89 In the absence of coronin-1C, the effect of caveolin-mediated endocytosis,

90 We find that coronin-1C-mediated extraction, which is responsible for

91 Unlike constitutive coronin-1C-mediated trafficking, caveolin-mediated Rac1

92 Here we provide evidence that coronin 2A (CORO2A), a component of the NCoR complex of

93 al microtubules become enriched in actin and coronin [6].

94 degradable, K33-linked polyubiquitination on coronin 7 (Crn7), which facilitates Crn7 targeting to TG

95 the TGN through K33-linked ubiquitination of coronin 7.

96 ole for the evolutionarily conserved protein coronin A in the initiation of the cAMP response.

97 These results suggest that coronin A is dispensable for cAMP sensing, chemotaxis, a

98 On starvation, coronin A-deficient cells failed to up-regulate the expr

99 Of importance, external addition of cAMP to coronin A-deficient cells resulted in normal chemotaxis

100 ng a functional cAMP relay in the absence of coronin A.

101 tions, some phagosomes showed persistence of coronin about the surface of the compartment, but coloca

102 nin isoforms suggests that the nature of the coronin-actin association may be similar in other family

103 s that express GFP-tagged fusion proteins of Coronin and actin-interacting protein 1, as well as knoc

104 ein 1, as well as knockout mutants that lack Coronin and actin-interacting protein 1.

105 Together, coronin and Aip1 lower the amount of cofilin required to

106 n assays revealed an association of Ndm with coronin and F-actin.

107 ve inhibitory effects on Arp2/3 complex with Coronin and GMF.

108 s, a localization pattern that is similar to coronin and partly dependent on RacE.

109 of the biochemical and cellular functions of coronin and that the beta-propeller domain mediates addi

110 xture of actin disassembly factors (cofilin, coronin, and actin-interacting protein 1 is sufficient t

111 sembly of single actin filaments by cofilin, coronin, and actin-interacting protein 1, a purified pro

112 The cooperative activities of cofilin, coronin, and Aip1 should provide a biochemical basis for

113 ith the Dictyostelium actin-binding protein, coronin, and approximately 67% homology with the previou

114 itory ligands: glia maturation factor (GMF), Coronin, and Arpin.

115 osin II and globally distributed dynacortin, coronin, and RacE.

116 Coronins are a conserved family of WD repeat-containing,

117 Coronins are evolutionarily conserved proteins that were

118 Coronins are F-actin-binding proteins that are involved,

119 Although coronins are known to play a role in controlling actin d

120 Coronin arrives last as all other components disperse up

121 chemical fractionation, we identify Aip1 and coronin as two proteins present in thymus extract that f

122 mutational analysis of surfaces on the yeast coronin beta-propeller domain, which binds to F-actin an

123 and Rac-interactive binding motif (CRIB) of coronin binds to Rho GTPases with a preference for GDP-l

124 Such a mode of binding explains how coronin can stabilize actin filaments in vitro.

125 It is rescued by wild-type coronin, whereas coronin carrying a mutated Cdc42- and Rac-interactive bi

126 tin networks have remained elusive; however, Coronin, Cofilin and AIP1 have been implicated in this p

127 ve investigated the mechanism by which yeast coronin (Crn1) enhances F-actin turnover.

128 Here, we show that yeast coronin (Crn1) makes a unique contribution to this proce

129 tailed biochemical analysis of budding yeast coronin, Crn1, and found that it not only inhibits Arp2/

130 aphy, we coisolated actin and a homologue of coronin, Crn1p, from Saccharomyces cerevisiae cell extra

131 ults in increased 1evels of activated Rac in coronin-deficient Dictyostelium cells (corA(-)), which i

132 and cell adhesion components (e.g., catenin, coronin, dystrobrevin, and syndecan), consistent with it

133 In other cell types, coronins exert their effects on lamellipodia dynamics by

134 ich binds to F-actin and is conserved in all coronin family members.

135 is the hematopoietic-specific member of the Coronin family of actin regulators that promote F-actin

136 this protein, POD-1, as a new member of the coronin family of actin-binding proteins.

137 An understanding of coronin function has been hampered by the absence of any

138 iquitously expressed member of the mammalian Coronin gene family that co-localizes with the Arp2/3 co

139 FP-dynacortin and the actin bundling protein coronin-GFP are seen to concentrate in highly dynamic co

140 We therefore examined whether yeast lacking coronin had defects in the microtubule cytoskeleton.

141 nts that lack coronin, yeast strains lacking coronin had no detectable defects in actin-based process

142 rved disassembly-promoting factors including coronin have remained more obscure.

143 that contains sequences (not found in other coronins) homologous to the microtubule binding region o

144 Despite emerging roles for coronin in a range of physiological processes and diseas

145 Furthermore, GMF synergized with Coronin in inhibiting actin nucleation by Arp2/3 complex

146 be the identification of a single homolog of coronin in Saccharomyces cerevisiae, which we show local

147 However, the existence and role of coronins in vascular smooth muscle cell (VSMC) migration

148 n 1A in T cell egress, identify a surface of coronin involved in Arp2/3 regulation and demonstrate th

149 er, these results suggest that S. cerevisiae coronin is a component of the actin cytoskeleton that ma

150 Coronin is a conserved actin-binding protein that co-fun

151 Coronin is a highly conserved actin-associated protein t

152 More recent studies have revealed that coronin is involved in actin-based motility, cytokinesis

153 y during the initiation of the response, and coronin is recruited as the actin filaments are disassem

154 icating that activation of Arp2/3 complex by coronin is required for normal actin dynamics in vivo.

155 nt species and in all three major classes of coronin isoforms suggests that the nature of the coronin

156 with the previously cloned human and bovine coronin-like homologue, p57.

157 ing proteins and the F-actin-binding protein coronin localize to its leading edge, but in an shk1 nul

158 Coronins make up a large class of actin-binding proteins

159 The coronin mutant and the myoA/B double myosin I mutant wer

160 n mouse macrophages, a defined D. discoideum coronin mutant was analyzed.

161 ud neck, failed to reveal any defects in the coronin mutant.

162 wed normal actin binding but failed to cause coronin overexpression defects.

163 ion to actin structures in vivo, and loss of coronin overexpression growth defects.

164 s (2016) show that the actin binding protein Coronin plays a critical role in actin cytoskeleton reor

165 We conclude that, analogous to coronin, POD-1 plays an important role in intracellular

166 We conclude that coronin polarizes the spatial distribution and activity

167 with the N-terminal beta-propeller domain of Coronin positioned near the p35/ARPC2 subunit of Arp2/3

168 Coronin promoted a standard (previously described) open

169 t study was to define the mechanism by which coronins regulate platelet-derived growth factor (PDGF)-

170 Surprisingly, the absence of coronin resulted in enhanced intracellular replication o

171 ude that in D. discoideum factors other than coronin support intracellular replication of M. marinum.

172 We propose that coronin through its affinity for GDP-Rac regulates the a

173 Coronin was originally identified as a cortical protein

174 It is rescued by wild-type coronin, whereas coronin carrying a mutated Cdc42- and R

175 To test the role of host coronin, which was previously hypothesized to positively

176 derstanding of the molecular interactions of coronin with actin and other binding partners has been l

177 Unlike Dictyostelium mutants that lack coronin, yeast strains lacking coronin had no detectable