ライフサイエンスコーパス: cortical granule

1 from the cell surface, rather than from the cortical granules.

2 f the ras superfamily, rab3, were present on cortical granules.

3 o hyalin, a protein packaged specifically in cortical granules.

4 extracellular surface of the egg and to the cortical granules.

5 zation some of the antigens are localized to cortical granules.

6 t with exocytosis of proteoglycan-containing cortical granules.

7 underlying the oolemma, deep to the zone of cortical granules.

8 tosis, myosin 1e relocates to the surface of cortical granules.

9 ertilization are driven by the exocytosis of cortical granules.

10 of the overexpressed csp was associated with cortical granules.

11 onsistent with the dynamics of exocytosis of cortical granules.

12 opulation of targets for cleavage within the cortical granules.

13 les in the egg cortex that are distinct from cortical granules.

14 with Lens culinaris agglutinin, a marker for cortical granules.

15 e followed by positional quantitation of the cortical granules.

16 heral Golgi complexes, and a failure to form cortical granules.

17 In the sea urchin, cortical granules accumulate throughout the cytoplasm du

18 Ovastacin is a pioneer component of mouse cortical granules and plays a definitive role in the pos

19 s occur because of defects in trafficking of cortical granules and the subsequent formation of an imp

20 only when secreted, due to the low pH of the cortical granules, and it has a small population of targ

21 d in specialized secretory organelles called cortical granules, and PmARCs accumulated in a different

22 rchin egg, in which thousands of homogeneous cortical granules are associated with the plasma membran

23 In the in vitro-matured egg, 99% of the cortical granules are at the cortex, indistinguishable f

24 asm during oogenesis, but in mature eggs the cortical granules are attached to the plasma membrane, h

25 ertilization in sea urchins, the contents of cortical granules are secreted and rapidly assemble into

26 Cortical granules are secretory vesicles poised at the c

27 Cortical granules are specialized organelles whose conte

28 Cortical granules are synthesized throughout oogenesis a

29 e, is maternally derived and exocytosed from cortical granules at fertilization.

30 However, just after GVBD, cortical granules attach to microfilaments and transloca

31 pecific antiserum, ovastacin was detected in cortical granules before, but not after, fertilization.

32 ected vesicle hitchhiking mechanism by which cortical granules bind to Rab11a vesicles powered by myo

33 We determined the pH of the cortical granule by fluorescent indicators and micro-pH

34 peared from these structures and appeared on cortical granules by anaphase I.

35 active forms of the protease, is detected in cortical granules by immunoblot analysis.

36 V (CaMKIV) is highly expressed in cerebellar cortical granule cells and deep nuclear neurons in the c

37 s of many species are incompetent to undergo cortical granule (CG) exocytosis upon fertilization.

38 ena of ooplasmic microtubule dynamics (OMD), cortical granule (CG) exocytosis, zona pellucida (ZP) ha

39 a(2+) transients resulted in 39 and 49% mean cortical granule (CG) loss, respectively, while CG exocy

40 luding reduced cortical integrity, defective cortical granule (CG) release upon egg activation, failu

41 equired for normal egg Ca2+ oscillations and cortical granule (CG) secretion.

42 luding ooplasmic microtubule dynamics (OMD), cortical granule (CG) status, and zona pellucida (ZP) ha

43 ugh a chromatin-induced localized release of cortical granules (CG) during maturation is thought to b

44 In activated Xenopus eggs, exocytosing cortical granules (CGs) are surrounded by actin "coats,"

45 actin that surround and compress exocytosing cortical granules (CGs).

46 Zymography assays suggest that the cortical granules contain a single serine protease that

47 ndergo a selective turnover of mRNA encoding cortical granule contents.

48 As constituents of the membrane of cortical granules, cysteine string proteins are also ess

49 thylmaleimide inhibition, we determined that cortical granules do have spare active fusion complexes

50 are functional prior to meiosis, even though cortical granules do not use them.

51 Cortical granules exocytose after the fusion of egg and

52 al stage, egg activation, including abnormal cortical granule exocytosis (CGE), cytoplasmic segregati

53 3 or GalTase antibodies were able to trigger cortical granule exocytosis and activation of GalTase-ex

54 Among these, cortical granule exocytosis and compensatory endocytosis

55 the protease-mediated reactions that follow cortical granule exocytosis and contribute to the block

56 subsequent egg activation events, including cortical granule exocytosis and cytoplasmic segregation.

57 rable yet coordinated functions: to regulate cortical granule exocytosis and to mediate chromosome se

58 e plasma membrane in a manner reminiscent of cortical granule exocytosis as described in other specie

59 n 1e is expressed at the earliest stage that cortical granule exocytosis can be detected in oocytes.

60 Interestingly, cortical granule exocytosis can be elicited in immature

61 ean that rab3 functions in the regulation of cortical granule exocytosis following vesicle docking.

62 nt regions of the rab3 protein, we find that cortical granule exocytosis is inhibited in eggs injecte

63 We find that whereas cortical granule exocytosis occurs over a narrow thresho

64 d overexpression of full-length Xenopus csp, cortical granule exocytosis was reduced by approximately

65 IP(3)-induced cortical granule exocytosis was significantly decreased

66 ecies, binding of sperm to the egg initiates cortical granule exocytosis, an event that contributes t

67 From the calcium dependence of cortical granule exocytosis, and from the exposure time

68 ries of Ca(2+)-dependent events that include cortical granule exocytosis, cell cycle resumption with

69 W-7 followed by insemination does not block cortical granule exocytosis, cell cycle resumption, as a

70 nhibition of Ca2+ release and also inhibited cortical granule exocytosis, cytoplasmic alkalinization,

71 phatase SHP2, had no effect on Ca2+ release, cortical granule exocytosis, DNA synthesis, or cleavage.

72 Some events of egg activation, e.g., cortical granule exocytosis, however, appear more sensit

73 gradation and recruitment of maternal mRNAs; cortical granule exocytosis, however, did not occur norm

74 Following cortical granule exocytosis, however, rab3 reassociates

75 intracellular Ca2+, resume meiosis, undergo cortical granule exocytosis, or ZP2 cleavage to ZP2f.

76 lthough the native J-domain of csp inhibited cortical granule exocytosis, point mutations that interf

77 buffers and then examined the effect on the cortical granule exocytosis, recruitment of maternal mRN

78 g activation," resulting in calcium release, cortical granule exocytosis, recruitment of maternal mRN

79 rane fusion events such as sperm-egg fusion, cortical granule exocytosis, the elevation of phosphatid

80 ession of myosin 1e augments the kinetics of cortical granule exocytosis, whereas tail-derived fragme

81 all subsequent egg activation steps, such as cortical granule exocytosis, which modifies the vitellin

82 cytes, we hypothesize that rab3 functions in cortical granule exocytosis.

83 about 30 sec, or roughly at the same time as cortical granule exocytosis.

84 ith the calcium dependence of sea urchin egg cortical granule exocytosis.

85 l Zp2(Mut) embryos despite fertilization and cortical granule exocytosis.

86 teine string proteins are also essential for cortical granule exocytosis.

87 documents an important role for myosin 1e in cortical granule exocytosis.

88 rm binding, independent of fertilization and cortical granule exocytosis.

89 lar injection of myosin 1e antibody inhibits cortical granule exocytosis.

90 p) in phorbol-12-myristate-13-acetate-evoked cortical granule exocytosis.

91 A cortical granule-free domain (CGFD) overlies the metapha

92 rized egg, characterized by an actin cap and cortical granule-free domain (CGFD) overlying the meioti

93 Finally, the single cortical granule-free domain that normally becomes local

94 In these instances a cortical granule-free domain was observed over each indi

95 he spindle, dictates the localization of the cortical granule-free domain.

96 ganization is associated with non-exocytosed cortical granules from the earlier egg activation defect

97 The role of cortical granules in blocking polyspermy is conserved th

98 We identified cortical granules in Caenorhabditis elegans and have fou

99 Because of its selective association with cortical granules in eggs and oocytes, we hypothesize th

100 We found that the translocation of cortical granules in in vitro-matured oocytes begins wit

101 Exocytosis of cortical granules in mouse eggs is required to produce t

102 ex during oocyte maturation and anchoring of cortical granules in the cortex.

103 own, set of vesicles, closely apposed to the cortical granules in the egg cortex.

104 We then observed the translocation of cortical granules in these cells by immunolocalization u

105 Cortical granule lectin (CGL) is released by the egg of

106 abbit polyclonal Ab to Xenopus laevis 35-kDa cortical granule lectin (XL35; 68% identity with intelec

107 was unknown, namely, the Xenopus laevis egg cortical granule lectin.

108 th anti-receptor antibody are present in the cortical granules located at the inner face of the plasm

109 Fluorescently labeled cortical granules, microfilaments, and microtubules were

110 It was observed that cortical granules migrate to the periphery of the oocyte

111 aments and microtubules in the regulation of cortical granule movement toward the cortex during oocyt

112 gand is not a result of contamination by egg cortical granules, nor is it the mouse homolog of oviduc

113 otease 1 (CGSP1), has been identified in the cortical granules of Strongylocentrotus purpuratus eggs,

114 L receptor-like domains each localize to the cortical granules of unfertilized eggs.

115 r work indicated that csp is associated with cortical granules of Xenopus oocytes.

116 Cortical granules, once anchored at the cortex, remained

117 pliced to yield proteins destined for either cortical granules or the vitelline layer.

118 ion envelope at fertilization and changes in cortical granule pH.

119 eolytic cleavage of the gp69/64 protein by a cortical granule protease during fertilization removes 2

120 low-quality oocytes may undergo insufficient cortical granule release and zona-hardening, causing alt

121 distinctly trafficked variants reunite after cortical granule secretion at fertilization.

122 An autocatalytic serine protease, cortical granule serine protease 1 (CGSP1), has been ide

123 ino-acid-residue protein that we refer to as cortical granule serine protease 1 (CGSP1).

124 Here we show that a cortical granule serine protease of sea urchins is the m

125 ized insulin and reveal that the motility of cortical granules significantly changes over time.

126 This accumulation of csp at sites other than cortical granules suggested that mislocalized csp might

127 P3 are blocked in their ability to exocytose cortical granules, suggesting that the inhibition is dir

128 We find that native cortical granule targets of the protease are beta-1,3 gl

129 ts in the exocytosis of approximately 15,000 cortical granules that are docked at the plasma membrane

130 is cleavage is associated with exocytosis of cortical granules that are peripherally located subcellu

131 (3) Cortical granules that translocate in vitro are capable

132 ng oocytes coincident with the appearance of cortical granules, the vesicle in which the hyalin prote

133 Here we report that rab3 is associated with cortical granules throughout oogenesis, during cortical

134 ory step in the coordinated translocation of cortical granules to the egg cortex.

135 ral of these genes by RNAi, we observed that cortical granule trafficking was disrupted and the eggsh

136 iameter (Lytechinus variegatus), many of the cortical granules translocate upwards of 60 micro m to r

137 ation enables us to examine the mechanism of cortical granule translocation and other processes that

138 ings to discover that meiotic maturation and cortical granule translocation are inseparable.

139 We also learned that cortical granule translocation requires association with

140 Inhibiting cortical granule translocation severely impaired the blo

141 To study the process of cortical granule translocation to the cell surface we ha

142 rtical granules throughout oogenesis, during cortical granule translocation, and while docked at the

143 very little is known about the mechanism of cortical granule translocation.

144 Here we investigate cortical granule transport and dynamics in live mammalia

145 In many organisms, cortical granules undergo exocytosis following fertiliza