ライフサイエンスコーパス: cosedimentation

1 -actin in the presence of ATP, as assayed by cosedimentation.

2 ld localization and sucrose density gradient cosedimentation analyses to confirm association of smiti

3 Cosedimentation analysis and electron microscopy suggest

4 entified as a microtubule binding protein by cosedimentation analysis in the presence of microtubules

5 Furthermore, high speed cosedimentation analysis of dystrophin-glycoprotein comp

6 varying concentrations of NEM (0-10 mM) and cosedimentation and ATPase assays were used to assess th

7 T proteins were analyzed in microtubule (MT) cosedimentation and bundling assays to identify which ta

8 Using cosedimentation and coimmunoprecipitation we have define

9 -actin mRNA simultaneously in vitro in actin cosedimentation and enzyme-linked immunosorbent assays.

10 We assessed Tau-MT interactions via cosedimentation and found that the measured affinity of

11 Using a combination of glycerol gradient cosedimentation and immunoprecipitation analyses, we sho

12 Cosedimentation and limited proteolysis experiments indi

13 eractions with individual actin protomers in cosedimentation and solid-phase binding assays.

14 Purified K2 interacts with F-actin in cosedimentation and surface plasmon resonance analyses a

15 tin mutations on myosin binding, detected by cosedimentation, and actin structural dynamics, detected

16 V N protein was confirmed by immunoblotting, cosedimentation, and confocal microscopy.

17 confirmed by reciprocal immunoprecipitation, cosedimentation, and cotransfection analyses, and intera

18 By ELISA, cosedimentation, and surface plasmon resonance using pho

19 Results from the in vitro cosedimentation assay indicated that UNC5C interacted wi

20 Here, we show by using a cosedimentation assay that MAP2c binds F-actin.

21 In the cosedimentation assay, treatment with </=0.1 mM NEM enha

22 ive in binding to peptidoglycan sacculi in a cosedimentation assay.

23 ties, had diminished ability to bind RT in a cosedimentation assay.

24 Cosedimentation assays and pyrene-actin fluorescence exp

25 actin in a concentration-dependent manner in cosedimentation assays and that BimA stimulates actin po

26 D. rerio alphaE-catenin binds F-actin in cosedimentation assays as a monomer and as an alpha/beta

27 that alphaT-catenin monomer binds F-actin in cosedimentation assays as strongly as alphaE-catenin hom

28 nking and/or bundling, which was observed in cosedimentation assays as well as by low shear viscometr

29 Coimmunoprecipitation and F-actin cosedimentation assays demonstrate that PDZ-RhoGEF binds

30 Using tissue-based cosedimentation assays on mice expressing endogenous dys

31 Cosedimentation assays performed with these domains expr

32 Actin cosedimentation assays show this is caused by direct com

33 Cosedimentation assays showed that a peptide containing

34 actin ligand blotting and high-speed F-actin cosedimentation assays to analyze the F-actin binding pr

35 epolymerization assays, and microtubule.MCAK cosedimentation assays to compare the activity of full-l

36 We employ actin cosedimentation assays to show that Arg increases the st

37 opsis KCBP and used the purified proteins in cosedimentation assays with microtubules.

38 Results of the cosedimentation assays with the N-terminal tail suggest

39 (residues 1247-1495) in glutathione-agarose cosedimentation assays, even though the CaMKII-binding d

40 mplex were examined using high and low speed cosedimentation assays, microcapillary falling ball visc

41 actin networks by using imaging techniques, cosedimentation assays, multiparticle tracking, and bulk

42 Using cosedimentation assays, we determined that KRP binds to

43 2p is a nonprocessive motor comes from actin cosedimentation assays, which show that Myo2p has a low

44 an actin binding domain defined by in vitro cosedimentation assays.

45 Here we show using cosedimentation binding assays, that the 4 N-terminal do

46 nephrin, podocin, CD2AP, ZO-1, and Neph1 by cosedimentation, coimmunoprecipitation, and pull-down as

47 F-actin cosedimentation demonstrated that the nebulette fragment

48 The level of sigma(A)-RNAP cosedimentation dropped to less than 10% in a strain whi

49 med mixed bipolar filaments, as evidenced by cosedimentation, electron microscopy, and single-molecul

50 Immunofluorescence microscopy and MT cosedimentation experiments demonstrate that the binding

51 Cosedimentation experiments revealed an approximately 3-

52 Cosedimentation experiments showed that gamma(1) and alp

53 , but not CAp24, was able to bind F-actin in cosedimentation experiments.

54 C2 was detected by coimmunoprecipitation and cosedimentation experiments.

55 Cosedimentation, immunoprecipitation, and actin binding

56 By cosedimentation, maximum filament formation occurs at a

57 Studies of the cosedimentation of 125I-34 kDa protein and F-actin show

58 ture of an effective particle of the coupled cosedimentation of an interacting system.

59 occus aureus protease (myosin S1SA) inhibits cosedimentation of CaP with myosin filaments.

60 reatment, and (iv) coimmunoprecipitation and cosedimentation of Dnmt3b specifically with Hdac2 in a g

61 Finally, nuclear fractionation studies show cosedimentation of GRWD1 with preribosomal complexes, as

62 mutations to cysteine significantly disrupt cosedimentation of headpiece with F-actin.

63 Cosedimentation of human APOBEC3G and intracellular Gag

64 nal spread of the reaction boundary from the cosedimentation of interacting macromolecules, which als

65 ty of protein, thus eliminating coincidental cosedimentation of protein aggregates with mitochondria.

66 Cosedimentation of rabbit skeletal muscle and yeast F-ac

67 The F-actin affinity (measured by cosedimentation) of Glu180Gly was similar to that of wil

68 try, coelution from a gel filtration column, cosedimentation on a glycerol gradient, and in vitro pro

69 Using a combination of cosedimentation, overlay, and direct binding assays, we

70 We used a combination of cosedimentation, overlay, and fluorescence assays to det

71 Cosedimentation persisted even after deproteinization.

72 This cosedimentation represents bona fide galectin-3--snRNP c

73 The cosedimentation results were supported by viscometry res

74 In vitro cosedimentation studies also demonstrated that nebulin S

75 Cosedimentation studies show that the N-terminal region

76 s were defined by actin affinity measured by cosedimentation, troponin T affinity using a newly devel

77 der denaturing and nondenaturing conditions, cosedimentation, viscometry, and pyrene-labeled actin di

78 are traditionally determined by centrifugal cosedimentation with actin microfilaments, where bound p

79 Cosedimentation with diatoms accumulated contaminants th

80 vages with proteases, followed by high-speed cosedimentation with F-actin and mass spectrometry, we f

81 an the intact protein aa1-295 as assessed by cosedimentation with F-actin.

82 Caspase 3 binding to PIP2 was confirmed by cosedimentation with mixed lipid vesicles.

83 Immunoblotting revealed an EDTA-dependent cosedimentation with ribosomes in sucrose gradients for

84 In vitro cosedimentation with taxol-stabilized microtubules demon