1 enhanced by retinoid X receptor-alpha (RXR)
cotransfection.
2 ction of luciferase activity with HIF-2alpha
cotransfection.
3 re upregulated 16- and 9-fold by pCI-Klf4 in
cotransfections.
4 Cotransfection analyses confirmed that both STAT5-bindin
5 By
cotransfection analyses of shotgun DNA fragments of a ba
6 al immunoprecipitation, cosedimentation, and
cotransfection analyses, and interaction sites were mapp
7 how that gene activity is dependent upon the
cotransfection and activation of Stat3.
8 Cotransfection and ChIP assays show that tumor growth fa
9 Cotransfection and ChIP assays were used to determine tr
10 Using
cotransfection and coimmunoprecipitation, we found that
11 bsolute stress sensitivity was calibrated by
cotransfection and comparison with MscL, a well-characte
12 Cotransfection and electrophoretic mobility shift analys
13 Cotransfection and fluorescence imaging have also confir
14 rmed immunoprecipitation following transient
cotransfection and found that STAT3 physically interacte
15 DNA
cotransfection and gel retardation demonstrated that vim
16 colocalized with AIRE in nuclear bodies upon
cotransfection and in human mTECs in situ.
17 Moreover, well-controlled
cotransfection and metabolic labeling experiments reveal
18 Through additional two-hybrid experiments,
cotransfection and reciprocal coprecipitation, glutathio
19 ntegration in vivo, viruses were prepared by
cotransfection and various IN-E2C fusion proteins were p
20 a direct target of Foxa2, as demonstrated by
cotransfection as well as in vivo chromatin immunoprecip
21 Furthermore, a protoplast
cotransfection assay showed that BBX24 and BBX25 repress
22 Moreover, in a
cotransfection assay, the p.His455Tyr mutant protein ret
23 n-defective mutant virus in an HSV viral DNA
cotransfection assay.
24 the ability to enhance UL84 expression in a
cotransfection assay.
25 ionally transactivated by ICP27 in a plasmid
cotransfection assay.
26 Transient
cotransfection assays demonstrate that PGC-1alpha augmen
27 Transient
cotransfection assays demonstrated that ER alpha was a m
28 Cotransfection assays demonstrated that the transfection
29 Moreover, chromatin immunoprecipitation and
cotransfection assays established the P2 intronic promot
30 In vivo dephosphorylation and
cotransfection assays reveal that PTP1B binds to VEGFR2
31 retic mobility shift assays, supershift, and
cotransfection assays revealed that the activation of p6
32 ciation between HNF6 and FoxM1 proteins, and
cotransfection assays show that HNF6 stimulates Foxm1 tr
33 Cotransfection assays show that hnRNPE2 isoforms moderat
34 We demonstrate using
cotransfection assays that the Kir6.2 gene is a transcri
35 Here, we used HepG2 cell
cotransfection assays to demonstrate that HNF6 transcrip
36 MP-1 promoter reporter plasmids in transient-
cotransfection assays, and to rescue LCL growth followin
37 In
cotransfection assays, Brn-3b can strongly transactivate
38 autoregulate its own expression in transient
cotransfection assays, but there is conflicting evidence
39 In transient
cotransfection assays, ectopic expression of wild type E
40 olog of ORF57, had a similar activity in the
cotransfection assays, herpes simplex virus type 1 ICP27
41 lectrophoretic mobility shift and luciferase
cotransfection assays, revealed that the N46H variant ca
42 In
cotransfection assays, SENP5 preferentially reduced high
43 Using mutagenesis, EMSA, and
cotransfection assays, we identified two redundant Runx
44 y expressed ERalpha when tested in transient
cotransfection assays.
45 P interacts with LANA in BCBL-1 cells and in
cotransfection assays.
46 d C/EBPalpha-HNF6 transcriptional synergy in
cotransfection assays.
47 activation of the -5.3-kb Foxf1 promoter in
cotransfection assays.
48 P7A1 transcriptional stimulation by HNF-6 in
cotransfection assays.
49 29ab1 promoter-driven luciferase activity in
cotransfection assays.
50 on IE2 86 and IE2 40 protein levels in these
cotransfection assays.
51 romoter activities was measured by transient
cotransfection assays.
52 vities were upregulated by Klf4 in transient
cotransfection assays.
53 In addition,
cotransfection data suggest that parafibromin can intera
54 d that mutated VE-cadherin 3'UTR and miR-101
cotransfection did not change luciferase activity.
55 moter, A-296C and A-261T, using transfection/
cotransfection,
electrophoretic mobility shift assay (EM
56 From results of the
cotransfection experiment, we concluded that AtERF53 has
57 Cds1 gene promoter-reporter
cotransfection experiments and chromatin immunoprecipita
58 uciferase activity measurements in transient
cotransfection experiments and electromobility shift ass
59 nteraction of L2 protein with syntaxin 18 in
cotransfection experiments and resulted in noninfectious
60 Cotransfection experiments and Sp inhibitor studies demo
61 Cotransfection experiments demonstrate that miR-146a's r
62 Cotransfection experiments demonstrated that overexpress
63 Cotransfection experiments extended the last finding by
64 In vitro
cotransfection experiments in A549 cells demonstrated th
65 Cotransfection experiments in DU145 and U87 cells demons
66 Cotransfection experiments indicate that TALE proteins a
67 Cotransfection experiments revealed that iASPP, but not
68 Cotransfection experiments revealed that overexpression
69 Cotransfection experiments revealed that UL16 and VP22 c
70 Cotransfection experiments showed that KLF4 overexpressi
71 Mutagenesis and
cotransfection experiments showed that PKA regulation of
72 These
cotransfection experiments showed that rs174545 (FADS1:m
73 , we utilized a functional budding assay and
cotransfection experiments to examine the contributions
74 Cotransfection experiments using promoter-reporter const
75 In
cotransfection experiments with a wild-type fXI construc
76 SOX9 was sumoylated in
cotransfection experiments with COS-7 cells using PIAS a
77 Cotransfection experiments with full-length HBV and DNMT
78 Since this was not observed in
cotransfection experiments with Grb2 and PLD2-Y169/179F,
79 Cotransfection experiments with hsa-miR-107 oligonucleot
80 Cotransfection experiments with promoter constructs reve
81 Consistent with the results of
cotransfection experiments, a point mutation at the -3 p
82 Furthermore, in
cotransfection experiments, a secretion-defective PD del
83 In
cotransfection experiments, corepressors were excluded f
84 In
cotransfection experiments, increase in epsilon-promoter
85 te transcription of the Itgbeta3 promoter in
cotransfection experiments, indicating that the mouse It
86 epression of K1p transcriptional activity in
cotransfection experiments.
87 gative effects on wild-type fXI secretion in
cotransfection experiments.
88 ional activity of the mouse CCR2 promoter in
cotransfection experiments.
89 ing the destruction of target transcripts in
cotransfection experiments.
90 Using
cotransfection,
gel mobility shifts, and DNase I footpri
91 Cotransfection in HEK293T cells of mouse or human PM20D2
92 odulatory effects of TLR10: on the one hand,
cotransfection in human cell lines showed that TLR10 act
93 nteract in vitro and in vivo and that SECp43
cotransfection increases this interaction and redistribu
94 in vivo intermolecular competition following
cotransfection into cells, between two sequence-marked H
95 To study this further,
cotransfection into HBE cells of wild-type or mutant UBE
96 odel system for analysis, using WIP and WASP
cotransfection into Jurkat cells, in which strong induct
97 In addition, SUMO-1
cotransfection led to augmented Oct4 transactivation pot
98 Using this
cotransfection method with mutagenesis study, we identif
99 mounts of a K-bZIP expression plasmid in the
cotransfection mixture or by dominant-negative inhibitio
100 Cotransfection,
mutagenesis, and gel retardation experim
101 e used artificially induced recombination by
cotransfection of 5'-end-deleted and 3'-end-deleted and
102 In addition,
cotransfection of a constitutively active mutant of Stat
103 Cotransfection of a construct expressing the Galpha(q) s
104 Cotransfection of a MAP4K1 promoter-reporter with Pdcd4
105 th cDNAs in murine C2C12 myoblasts following
cotransfection of a murine UCP3 promoter-luciferase cons
106 ylatable mutant, p27/Thr187A, are rescued by
cotransfection of a phosphorylation-mimicking mutant, p2
107 Tonic depletion of PIP(2) by
cotransfection of a PIP(2) phosphatase had no effect, an
108 We found that
cotransfection of a plasmid DNA encoding POSH stimulated
109 Cotransfection of a pp150 expression plasmid with DeltaU
110 This migration defect is rescued by
cotransfection of a rat MDGA1 expression construct along
111 ycle, a phenotype only partially restored by
cotransfection of a transcriptionally active form of bet
112 ptional activity was strikingly increased by
cotransfection of a wild-type p53 expression vector or t
113 enhanced by Sp1 protein, as demonstrated by
cotransfection of ABCC6 promoter-luciferase constructs a
114 Cotransfection of AGS cells with Muc1 plus IKKbeta, but
115 studied under perforated patch-clamp showed
cotransfection of AKAP79 to "sensitize" KCNQ2/3 heterome
116 Cotransfection of AKR1B10 with a luciferase reporter pla
117 Cotransfection of AktCA with Hsp27 short interfering RNA
118 logical characteristics that closely matched
cotransfection of alpha with beta subunits.
119 Cotransfection of ALX with LAX resulted in LAX tyrosine
120 Cotransfection of AMCase and EGFR also increased, wherea
121 ells, and Gag synthesis could be restored by
cotransfection of an env expression plasmid (DeltaGP).
122 he microRNA effect was further documented by
cotransfection of an hsa-miR-1294 mimic, yielding an exa
123 Cotransfection of an IL13 reporter construct with expres
124 Cotransfection of an inactive or an MTS-sensitive SERT w
125 IRF8 binds to the Mdm2 P2 promoter, and that
cotransfection of an IRF8 expression vector with an Mdm2
126 transactivate KSHV promoters independently,
cotransfection of an Rta mutant lacking its transactivat
127 Cotransfection of atrogin-1 in HEK293 cells significantl
128 Cotransfection of both receptors revealed a specific col
129 Cotransfection of BRCA1 and FOXA1 resulted in a synergis
130 Cotransfection of Ca(v)2.1 with the EF-hand Ca2+-binding
131 Cotransfection of CaM(MUT) with TRPM2 dramatically inhib
132 amin A), CaR expression levels are very low;
cotransfection of CaR with filamin A increases total cel
133 Cotransfection of CDO enhances the activity of the neuro
134 Cotransfection of cells with a plasmid encoding the domi
135 Cotransfection of cells with a plasmid that encodes E. c
136 by truncated GPR56 that could be rescued by
cotransfection of cells with beta-arrestin 2.
137 s shown by coimmunoprecipitation assays, and
cotransfection of cells with FOXO1 and HDAC3, but not HD
138 Cotransfection of cells with infectious viral DNA and pl
139 Cotransfection of cells with P450s and PGRMC1 resulted i
140 Finally,
cotransfection of COS-7 cells with rat or human UPF0586
141 Upon
cotransfection of COS7 cells with Frmpd1-GFP and AGS3-mR
142 Cotransfection of DC-SIGN or DC-SIGNR with HIV demonstra
143 Cotransfection of different isoforms further enhanced bi
144 Cotransfection of DNA-PKcs with Bax shRNA restored Bax s
145 But,
cotransfection of effector-loop mutants of Ras determine
146 ears to be physiologic, as reconstitution or
cotransfection of either cPLA(2) or PLD with PLCepsilon
147 In addition,
cotransfection of either E12 or E47 with Id1 led to a ma
148 In nonmuscle cells,
cotransfection of either E12 or E47 with MyoD extended M
149 Cotransfection of either Sp1 or Sp3 with a reporter driv
150 In cultured cells,
cotransfection of ephrinB1 with CNK1 increases JNK phosp
151 y relevant system, we have demonstrated that
cotransfection of ERBB4 and STAT5A in a human breast can
152 ce to support the above expression data: (1)
cotransfection of ERK5wt and MEK5D constructs in PC3 cel
153 Cotransfection of expression plasmids of human or mouse
154 bserved upon transfection of Flag-RINGmut or
cotransfection of Flag-gp78 with ubiquitin mutated at th
155 Cotransfection of fluorescent CNS reporter constructs an
156 Cotransfection of FOXA1 and BRCA1 resulted in a greater
157 Cotransfection of Fra-2 with the Jun AP-1 subunits and p
158 Cotransfection of FRalpha and PCFT resulted in enhanced
159 ssion model lead to the observation that the
cotransfection of G alpha12 and eNOS expression vectors
160 Cotransfection of GABARAP with an AT(1)R fluorescent fus
161 Cotransfection of genotypes G and A did not lead to mutu
162 ckdown impaired effects could be reversed by
cotransfection of GFP-tagged full-length GMFG.
163 Transient
cotransfection of green fluorescent protein-LC3 with eit
164 Cotransfection of HEK cells with both the truncated CYP2
165 Cotransfection of HEK-293 cells with SERT and a constitu
166 Transient
cotransfection of HeLa cells with GFP and SAT1 vectors s
167 .5-fold increase in activity was obtained by
cotransfection of hepatocyte nuclear factor (HNF) 3gamma
168 Cotransfection of HepG2 cells with a CYP3A4 proximal pro
169 Cotransfection of Hoxa10-1 together with Hoxa10-2 or Hox
170 hepatitis delta virus (HDV) was achieved by
cotransfection of Huh7 cells with two plasmids: one to p
171 Cotransfection of human Pdx-1 with a reporter fused to t
172 ystem to detect ISGylated target proteins by
cotransfection of ISG15, UBE1L, and Ubc8 together with a
173 Cotransfection of KL with either ADAM10 or ADAM17 enhanc
174 AM10 or ADAM17 enhances KL cleavage, whereas
cotransfection of KL with small interference RNAs specif
175 Cotransfection of miR-155 with CDC73 in HEK293 cells abr
176 As (miRNAs) regulate visual cycle genes, and
cotransfection of miRNA mimics with luciferase reporter
177 The impact of
cotransfection of mixtures of mutant and wild type (WT)
178 Cotransfection of neurofilament light chain (NEFL) and m
179 Cotransfection of NF-kappaB and AP-1 reporter constructs
180 This prediction was confirmed by the
cotransfection of NF-kappaB and C/EBPbeta and the IKK-NB
181 Cotransfection of NF-kappaB/p65 with Ly49g Pro1 in LNK c
182 cyclin D1 promoter activity was repressed by
cotransfection of NF2, and PAK activity was inhibited by
183 In contrast,
cotransfection of NFIB2, NFIC1, NFIC2, NFIX1, and NFIX2
184 Cotransfection of NFkappaB p50 and p65 cDNA induced 5-FU
185 e marker RNA Binding Protein 47 (RBP47) upon
cotransfection of Nicotiana benthamiana leaves.
186 Furthermore, the
cotransfection of NLS deficient mutants of MyoD or Id1 w
187 icantly, pulse-chase experiments showed that
cotransfection of Nrdp1 and parkin reduced the half-life
188 Cotransfection of ORF57 and K8beta cDNA, which retains a
189 Cloned RRV that was produced by
cotransfection of overlapping cosmids spanning the entir
190 Cotransfection of p50 completely blocked p65-mediated in
191 Cotransfection of p53 and Hausp stabilizes p53 through t
192 ine uptake that was effectively inhibited by
cotransfection of Par-4.
193 Transient
cotransfection of plasmids constitutively expressing gB
194 Cotransfection of plasmids expressing ORF9, IE62, and th
195 Cotransfection of PLM in HEK293 cells slowed Ca(V)1.2 cu
196 promoter in PRA-transfected cells; however,
cotransfection of PRA and PRB significantly decreased th
197 Cotransfection of RNA and an I-SceI expression vector de
198 Cotransfection of RPL4 cDNA with Moloney murine leukemia
199 Cotransfection of SERT with L90V-A(3)AR, a hyperfunction
200 Cotransfection of Smad2 and Runx2 constructs had a coope
201 Cotransfection of Sp factors (Sp1, 3, or 4) upregulated
202 In addition,
cotransfection of ST18 and a TNF-alpha or IL-1alpha repo
203 Furthermore, we show that
cotransfection of STAT6(B) and LITAF induces an interact
204 Cotransfection of TACE in EC-2 cells enhanced phorbol my
205 Cotransfection of the G1862T mutant with a replication-d
206 Vector packaging by
cotransfection of the gag-pol.NLS variant with wild-type
207 Cotransfection of the gamma2 subunit with alphabeta-tand
208 Cotransfection of the isolated Igalpha1 and Iglambda cDN
209 ined B cells expressing mCD8alpha:chIgalpha,
cotransfection of the mCD8alpha:chIgalpha construct, tog
210 Cotransfection of the proximal Ihh promoter with PR demo
211 Further activation was observed upon
cotransfection of the set of plasmids expressing the ent
212 Cotransfection of the two constructs diminished VIP enha
213 onal activities were strikingly increased by
cotransfection of the wt p53 gene.
214 After
cotransfection of this vector with a CD3 vector into the
215 Cotransfection of three large (up to 10.8 kb) piggyBac t
216 ependent DNA replication was observed by the
cotransfection of UL84 and BAC-IN84/Ep.
217 Furthermore,
cotransfection of USF and SREBP-1c with an FAS promoter-
218 Cotransfection of wild-type MyoD or Id1 with NLS deficie
219 Finally, patch-clamp experiments showed
cotransfection of wild-type, but not DN, CaM to prevent
220 TIRF/FRET experiments revealed
cotransfection of wild-type, but not dominant-negative (
221 Cotransfection of X11alpha had an additive effect on ave
222 Cotransfection of ZNF24 and a VEGF promoter luciferase r
223 However, in stable
cotransfections of 6xHis-tagged GPR17 with Myc-tagged Cy
224 he measles fusion (F) protein, using plasmid
cotransfection or bicistronic adenoviral vectors, the re
225 The original
cotransfection replication assay identified eight human
226 We developed a modification of the transient
cotransfection replication assay wherein both lytic (ori
227 In the transient-
cotransfection replication assay, K-Rta is the only nonc
228 Also, using the
cotransfection replication assay, overexpression of the
229 in a dose dependent manner when added to the
cotransfection replication assay.
230 On the other hand, small interfering RNA
cotransfection resulted in inhibition of proliferation.
231 In our first study, we used a
cotransfection strategy to express the components of the
232 Cotransfection studies demonstrated that FoxA2 protein b
233 Cotransfection studies demonstrated that Foxm1 stimulate
234 Cotransfection studies in a heterologous system show tha
235 In
cotransfection studies in COS7 cells and in transfection
236 Cotransfection studies indicated that ubiquilin 1 locali
237 We show here that in
cotransfection studies or in the reconstituted frog oocy
238 Cotransfection studies revealed that CArG elements were
239 Next, EMSA, immunoblots, and transient
cotransfection studies with reporter plasmids (pNF-kappa
240 In
cotransfection studies, alphaCP3 repressed the MOR promo
241 By
cotransfection studies, Sox4 is able to transactivate th
242 In
cotransfection studies, SR1848 reduced LRH-1-dependent e
243 We developed a
cotransfection system in which epitopically tagged Gag p
244 Cotransfection to express S100A8/A9 and CAMP together au
245 nscriptional regulatory elements, microarray
cotransfection was used to functionally characterize con
246 Transient
cotransfections were used to test whether Klf4 activates
247 s in ChREBP(-/-) hepatocytes was restored by
cotransfection with a ChREBP expression plasmid.
248 ity, and MUC2 transcription was inhibited by
cotransfection with a dominant-negative AP-1 vector.
249 We found that
cotransfection with a pHA-Hint1 plasmid DNA significantl
250 Cotransfection with a Smad3 expressing plasmid further r
251 is ordinarily inactive in 10T1/2 cells, but
cotransfection with a SOX9 expression plasmid was suffic
252 Cotransfection with a SUMO-expressing vector further enh
253 93T/TLR5 transfectants, which was blocked by
cotransfection with a TLR5 dominant-negative construct,
254 NADH application or
cotransfection with A280V GPD1-L resulted in decreased I
255 ability in a neuronal cell line by transient
cotransfection with A53T mutant alpha-syn.
256 t C (pGL3pCC) or T (pGL3pTT) with or without
cotransfection with an expression construct overexpressi
257 The activation was even greater upon
cotransfection with an upstream activator of mitogen-act
258 After
cotransfection with atrogin-1, the ubiquitination of Fla
259 ared with that of hSlo-beta(1)V146A, whereas
cotransfection with atrogin-1DeltaF (a nonfunctional mut
260 Cotransfection with both DN plasmids decreased phospho-p
261 lephrine and endothelin-1 stimulation and by
cotransfection with constitutively active CnA, NFATc4, a
262 Moreover,
cotransfection with constitutively active GSK-3beta prom
263 ANA promoter region by hypoxia as well as by
cotransfection with degradation-resistant HIF-1alpha or
264 Cotransfection with DeltaGP Y590F (mutant in the TM cyto
265 Cotransfection with dominant-negative PPAR gamma DNA eli
266 Additionally,
cotransfection with E2F1 and a dominant-negative c-myb i
267 ression of the UL84 protein is enhanced upon
cotransfection with either IE2 86 or IE2 40, although IE
268 polyphenol-induced eNOS activation required
cotransfection with ERalpha subject to phosphorylation a
269 At constant sEPO-R cDNA levels,
cotransfection with escalating asEPO-R cDNA further incr
270 In contrast,
cotransfection with fXI-Gly400Val or fXI-Trp569Ser reduc
271 ypoxia, which could be completely blocked by
cotransfection with HIF-1alpha siRNA.
272 f c-Jun and also AP-1 reporter activity, and
cotransfection with Hint1 inhibited both of these activi
273 Notably,
cotransfection with p50 completely blocked cytokine- and
274 ferase activity of reporter constructs after
cotransfection with Pax6 in COS7 cells.
275 ter activity was stimulated 7- to 10-fold by
cotransfection with pCI-Klf4.
276 Upon
cotransfection with pNL4-3.Luc molecular clone into 293T
277 lated region luciferase reporter activity on
cotransfection with precursor miR-423-5p (abolished by m
278 On
cotransfection with pUL47, a fraction of VHS-RNase was t
279 -Chinese hamster ovary cells; however, after
cotransfection with R1, these responses were suppressed.
280 Cotransfection with RTA blocked IRF7-mediated IFNalpha a
281 Cotransfection with Smad3 strongly induced promoter acti
282 apsigargin or tunicamycin is inhibited after
cotransfection with small inhibitory RNAs for Sp1, Sp3,
283 E-luciferase activity was fully prevented by
cotransfection with SRC-2, and partially prevented with
284 These properties were not affected by
cotransfection with SUR1.
285 RT transcription and c-myc expression, while
cotransfection with the corresponding antisense Smad3 co
286 4, or H15, and rescued HA reassortants after
cotransfection with the genes from either a low-pathogen
287 Cotransfection with the Hint1 plasmid DNA also inhibited
288 This enhancer element was transactivated by
cotransfection with the NF-kappaB subunit p65, alone or
289 tor benzamidine in the growth medium; or (2)
cotransfection with the physiological trypsin inhibitor
290 nsfected COS cells were greatly increased by
cotransfection with the scaffold/adapter protein beta-ar
291 ease in IC(50) for mutant variants following
cotransfection with the WT variant appear to be due to c
292 t increased the MAO B promoter activity, and
cotransfection with TIEG2 further increased the promoter
293 promoters in liver and intestinal cells upon
cotransfection with VDR.
294 -mediated activation was further enhanced by
cotransfection with Vdr.
295 les previously associated with Best disease,
cotransfection with wild-type bestrophin-1 did not impai
296 Cotransfection with wild-type lef-4 plasmid restored nor
297 Cotransfections with expression vectors encoding NF-kapp
298 RFdeltaC, produced the expanded phenotype in
cotransfections with H-Ras.
299 In contrast,
cotransfections with HPV-16 wt genomes that express phys
300 FACS with anti-Myc Ab was not reduced by the
cotransfection,
yet both LTD(4)-elicited ERK phosphoryla