ライフサイエンスコーパス: cotransformation

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1 This cotransformation ability was abrogated by deletions in C

2 We found that natural cotransformation allows scarless genome editing at unpre

3 atch the designed sequence using integrative cotransformation and clustered regularly interspaced sho

4 g a combination of Tn5 in vitro mutagenesis, cotransformation, and genetic complementation.

5 two independent transformation vectors for a cotransformation approach and two different selective ag

6 ng particle bombardment, we have developed a cotransformation approach in which spectinomycin-resista

7 es of p53 14/19 in the rat embryo fibroblast cotransformation assay.

8 Cotransformation assays using hBH deletion mutants revea

9 es critical to WhmD function, we developed a cotransformation-based assay to screen for alleles that

10 Nullizygous (-/-) cells were susceptible to cotransformation by adenovirus E1A plus activated H-Ras

11 Multigene cotransformation efficiency was correlated with the rati

12 5-hydroxyconiferyl alcohol were evidenced in cotransformation experiments, indicating that the introd

13 We report here a plastid cotransformation frequency of 50% to 64%, with a high fr

14 of interaction with TRP120 were confirmed by cotransformation in yeast.

15 we found no evidence for bias during natural cotransformation, indicating that this method can be use

16 Furthermore, we found that natural cotransformation is an effective method for multiplex ge

17 Cotransformation of a TAR vector and genomic DNA into ye

18 Cotransformation of an expression plasmid with pTara pro

19 lected marker, and we have demonstrated that cotransformation of an unselected marker on an independe

20 located in the first exon, are required for cotransformation of primary cells with an activated ras

21 urthermore, wild-type Mnt suppresses Myc+Ras cotransformation of primary cells, whereas Mnt containin

22 Cotransformation of the resultant plasmid, pTara, with o

23 Following cotransformation of the two fusion fragments into yeast,

24 ethod for accelerated evolution based on the cotransformation of unlinked genetic markers in naturall

25 Cotransformation of VHH-IgA with the porcine joining cha

26 Cotransformation of yeast with the target PAC or P1 clon

27 red for growth inhibition were mapped in Ras cotransformation or HepG2 hepatoma cell growth assays.

28 d into the same Arabidopsis genome either by cotransformation or through genetic crossing, hereby sig

29 nt cointegration of the marker gene, and the cotransformation-segregation approach.

30 In addition to the ability of gene cotransformation, this work demonstrates that the four-c

31 s reconstituted with the wild-type allele by cotransformation, virulence was restored.

32 ransient and stable modes of expression, and cotransformation was equally successful.

33 nsformation, as previously described for ras cotransformation, was not observed with v-src and second

34 he production of syringyl lignin in TEs, but cotransformation with COMT improved its formation.

35 es (MPsi) made using PCR amplification after cotransformation with GagDeltaPR protein into yeast cell

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