ライフサイエンスコーパス: cotranslational

1 Thus, neither posttranslational nor cotranslational acetylation of major H2A is essential.

2 t the turn motif (TM) site, Thr450, to avoid cotranslational Akt ubiquitination.

3 Here we identify cotranslational and posttranslational folding intermedia

4 the intact proteins revealed information on cotranslational and posttranslational modifications of t

5 ed glycosylation of proteins is an essential cotranslational and posttranslational protein modificati

6 IGNAL RECOGNITION PARTICLE54) is involved in cotranslational and posttranslational sorting of thylako

7 ane insertion of TM domains I-IX occurs in a cotranslational and sequential manner, while multiple to

8 Atp9-Oxa1 interaction is in contrast to the cotranslational and transient interaction previously obs

9 that this direct integration is obligatorily cotranslational, and by synthesizing stable ribosome-bou

10 SecY translocon complex is required for the cotranslational assembly of membrane proteins in bacteri

11 The cotranslational assembly of the IgE-binding alpha-chain

12 tably, loss of SSB dramatically enhances the cotranslational association of SSA with nascent chains,

13 d effectively as a CALI chromophore, and its cotranslational attachment to the target protein avoids

14 ings, it was possible to convert AQP1 into a cotranslational biogenesis mode similar to that of AQP4

15 ation of D1 protein turnover by facilitating cotranslational biosynthesis insertion (C-terminal domai

16 c-mediated transport and works best when the cotranslational branch of the pathway is employed.

17 on, indicating that the modification was not cotranslational but rather required caveolin-1 transport

18 ate that the profolding role of Hsc70 during cotranslational CFTR folding is counterbalanced by a dom

19 Because CFTR folding is predominantly cotranslational, changes in translational dynamics may p

20 ides are accurately sorted between the major cotranslational chaperone trigger factor (TF) and the es

21 s and of the canonical sequence required for cotranslational cleavage resembled the genetic organizat

22 For type II membrane proteins, these cotranslational constraints include N- and C-terminal me

23 egradome data demonstrated RRGD loci undergo cotranslational decay and identified changes in the ribo

24 g complex, reveal a role for this protein in cotranslational decay.

25 This pattern of cotranslational degradation is especially evident near t

26 ubiquitylation has been suggested to signal cotranslational degradation of nascent polypeptides, cot

27 Cotranslational degradation of polypeptide nascent chain

28 alpha) is required for ubiquitin-independent cotranslational degradation of the transcription factor

29 hat the Rel homology domain in p50 undergoes cotranslational dimerization and that this interaction i

30 tly, we discovered a previously unrecognized cotranslational down-regulation mechanism by which the n

31 We have investigated the cotranslational ER integration of a double-spanning prot

32 Accordingly, after the primary cotranslational event, additional posttranslational step

33 The disruption of core glycosylation, a cotranslational event, allows us to pinpoint these N-ter

34 n maturation process and shows how important cotranslational events contribute to the proper cellular

35 ysis of truncated chimeric proteins and (ii) cotranslational events of AQP1 biogenesis reflect a comm

36 To recapitulate the cotranslational events, including glycosylation, signal

37 To understand critical cotranslational events, the glycosylation and rate of tr

38 pid folding of thioredoxin in the cytoplasm; cotranslational export by the DsbA signal sequence avoid

39 Oxa1 is known to be required for cotranslational export of the Cox2 N-terminal domain acr

40 3 and the previously described selenoprotein cotranslational factors resulted in a model of selenocys

41 By combining S-acylation and cotranslational fatty acid chemical reporters with ortho

42 ses associated with protein biosynthesis and cotranslational folding (CTF).

43 iates predicted by a native-centric model of cotranslational folding account for the majority of thes

44 hether conformational constraints imposed by cotranslational folding affect the partitioning between

45 kinetics which reduces their probability of cotranslational folding and consequently increases the n

46 nonymous polymorphism, affects the timing of cotranslational folding and insertion of P-gp into the m

47 Hsp70 functions in both the cotranslational folding and posttranslational degradatio

48 e we present a kinetic formalism to describe cotranslational folding and predict the effects of varia

49 nced understanding of the molecular basis of cotranslational folding at atomic resolution.

50 The same effect is achieved by cotranslational folding at the ribosome in vivo.

51 in (RNC) complex is the major determinant of cotranslational folding behavior.

52 nslation rates can reduce the probability of cotranslational folding below that associated with arres

53 We tested whether this cotranslational folding can alter the folded structure o

54 The vectorial (N- to C-terminal) nature of cotranslational folding constrains the conformations of

55 The majority of these cotranslational folding domains are influenced by transl

56 ways and provide evidence that variations in cotranslational folding enable polytopic proteins to acq

57 ar proteins while directly measuring initial cotranslational folding forces.

58 fusion protein, we demonstrate an efficient cotranslational folding immediately after the emergence

59 slowly translated codons are associated with cotranslational folding intermediates, which may be smal

60 vitro system that allows direct analysis of cotranslational folding intermediates.

61 ontrast to refolding of a denatured protein, cotranslational folding is complicated by the vectorial

62 Our perspective is that cotranslational folding is the norm, but that the effect

63 integrated cellular response that shapes the cotranslational folding landscape at critical stages of

64 Cotranslational folding may be typical of small, intrins

65 mal mRNA sequences to control the simulated, cotranslational folding of a protein in a user-prescribe

66 Achieving efficient cotranslational folding of complex proteomes poses a cha

67 sociated cotranslational sequencing to track cotranslational folding of hemagglutinin in influenza A

68 equences evolved to fold in cells, including cotranslational folding of nascent polypeptide chains du

69 ur fixed lengths in vivo, in order to assess cotranslational folding of newly synthesized tailspike c

70 ich sequences act as "tRNA sponges" and help cotranslational folding of parasite proteins.

71 ation and open up possibilities to study the cotranslational folding of polytopic membrane proteins.

72 otein, modulate the rates of translation and cotranslational folding of protein domains monitored in

73 scence resonance energy transfer, we studied cotranslational folding of the first nucleotide-binding

74 Our observations show that cotranslational folding of the protein, which folds auto

75 cate an inverse correlation between CTPD and cotranslational folding on a proteome scale.

76 lationship between translation speed and the cotranslational folding probability is a general phenome

77 erone network likely underlies the elaborate cotranslational folding process necessary for the evolut

78 c formalism that we discuss can describe the cotranslational folding process occurring on a single ri

79 ing lengths, simulating intermediates in the cotranslational folding process.

80 tional design of mRNA sequences to guide the cotranslational folding process.

81 Ribosome-associated chaperones and cotranslational folding protect the majority of nascent

82 generality of fitness effects resulting from cotranslational folding remain open questions.

83 whose intrinsic properties hinder efficient cotranslational folding remain susceptible for cotransla

84 an intermediate or misfold, and examine the cotranslational folding scenarios that are possible with

85 We also determine possible cotranslational folding sites initiated by hydrophobic c

86 ctrum of nascent protein behaviors including cotranslational folding, aggregation, and translocation.

87 omputationally the existence of evidence for cotranslational folding, based on large sets of experime

88 n diverse functions, including regulation of cotranslational folding, covalent modifications, secreti

89 model and provide a molecular perspective on cotranslational folding, misfolding, and the impact of t

90 Escherichia coli cytosolic proteins exhibit cotranslational folding, with at least one domain in eac

91 s in facilitating polypeptide elongation and cotranslational folding.

92 overs evidence of evolutionary selection for cotranslational folding.

93 important for eukaryote-specific sequential, cotranslational folding.

94 lly important as pause sites in coordinating cotranslational folding.

95 and slow translation rates hinder efficient cotranslational folding.

96 AT ectodomain, with the initial and probably cotranslational formation of Cys-242-Cys-273, followed b

97 tilized in pulse-chase studies prevented the cotranslational formation of disulfides and when added p

98 The STT3B isoform is required for efficient cotranslational glycosylation of an acceptor site adjace

99 nsferase (OT), the enzyme that catalyzes the cotranslational glycosylation of asparagine.

100 T3A OST isoform is primarily responsible for cotranslational glycosylation of the nascent polypeptide

101 mer and for its allosteric regulation during cotranslational glycosylation.

102 in the mouse embryo through mTORC2-catalyzed cotranslational IMP1/IMP3 phosphorylation.

103 the DsbAss-promoted export of MBP is largely cotranslational, in contrast to the mode of MBP export w

104 Cotranslational incorporation of the coumarin derivative

105 The cotranslational incorporation of the unusual amino acid

106 and flash photolysis measurements after its cotranslational insertion into nanodiscs.

107 acting on transmembrane helices during their cotranslational insertion into the inner membrane of Esc

108 From the moment of cotranslational insertion into the lipid bilayer of the

109 erally thought to be determined during their cotranslational insertion into the membrane.

110 It is more effective in cotranslational insertion of membrane proteins and the p

111 Cotranslational insertion of membrane proteins into defi

112 hyll biosynthesis and the Sec/YidC-dependent cotranslational insertion of nascent photosystem polypep

113 eats, postulated to facilitate the psbA mRNA cotranslational insertion of the nascent D1 protein in t

114 Cotranslational insertion of the nascent peptide into th

115 drops may provide a hydrophobic interior for cotranslational insertion of the transmembrane helices a

116 Cotranslational insertion of type I collagen chains into

117 TMDs (DeltaG(app) > 0 kcal/mol) require the cotranslational insertion process for facilitating their

118 are occupied by translating ribosomes during cotranslational insertion, impeding the translocation of

119 During cotranslational integration of a eukaryotic multispannin

120 ernately into the cytosol or ER lumen during cotranslational integration.

121 t links ribosomes to thylakoid membranes for cotranslational integration.

122 chaperone trigger factor delays the onset of cotranslational interactions until the LuxB dimer interf

123 We describe here a technology, based on the cotranslational introduction of azide groups into protei

124 Overall the cotranslational inversion of marginally hydrophobic NA T

125 protein domains, allowing them to fold in a cotranslational-like sequential process.

126 fkb1 gene, p52 is principally generated in a cotranslational manner involving proteolytic processing

127 dly modifies newly synthesized proteins in a cotranslational manner.

128 exit site such that integration occurs in a cotranslational manner.

129 ed glycosylation, as well as other potential cotranslational maturation events.

130 Here, we characterized the cotranslational maturation pathway for the human type I

131 The cotranslational maturation process for the type II membr

132 nto operons therefore reflects a fundamental cotranslational mechanism for spatial and temporal regul

133 bacterial plasma membrane in an obligatorily cotranslational mechanism.

134 s is consistent with the vectorial nature of cotranslational membrane insertion found in vivo.

135 dase (COX) complex, where it facilitates the cotranslational membrane insertion of mitochondrially en

136 doplasmic reticulum (ER), where they undergo cotranslational membrane integration and assembly.

137 cross-link with Sf9 importin-alpha-16 during cotranslational membrane integration and remain proximal

138 that protein topology is established during cotranslational membrane integration.

139 at the I507-ATC-->ATT SCC contributes to the cotranslational misfolding, ERAD, and to the functional

140 bally slowed down, possibly due to increased cotranslational misfolding.

141 The cotranslational mixed-disulfide intermediate is, upon fu

142 the ribosome, thereby supporting a strictly cotranslational mode of action for eukaryotic SRP.

143 ogether, these results support a sequential, cotranslational model of archaeal polytopic membrane pro

144 For mitochondria, a cotranslational model of protein import is now proposed

145 Whereas processing of p40 conforms to the cotranslational model of signal peptide removal concomit

146 one of the limited examples of post- and/or cotranslational modification of proteins in this unusual

147 plex (TC) functions in the translocation and cotranslational modification of proteins made on membran

148 Cotranslational modification of the proteins by FGE prod

149 t two amino acids on proteins is a prevalent cotranslational modification.

150 primary cleavage site is suboptimal or that cotranslational modifications affect cleavage.

151 ase A (PKA-C) is subject to several post- or cotranslational modifications that regulate its activity

152 ranscripts to reveal the global landscape of cotranslational mRNA decay in the Arabidopsis thaliana t

153 Cotranslational mRNA degradation is initiated by decappi

154 is defective in cystic fibrosis, folds via a cotranslational multistep pathway as it is synthesized o

155 luding phosphorylation, N-glycosylation, and cotranslational myristoylation sites also punctuate the

156 Cotranslational N-glycosylation by the STT3A isoform of

157 Cotranslational N-glycosylation can accelerate protein f

158 is associated with the TC, and performs the cotranslational N-glycosylation of nascent polypeptide c

159 ligosaccharyl transferase (OT) catalyzes the cotranslational N-glycosylation of nascent polypeptides

160 nopeptidase (MetAP) carries out an important cotranslational N-terminal methionine excision of nascen

161 rwent posttranslational (rather than classic cotranslational) N-myristoylation when cleavage by caspa

162 p among a protein's amino acid sequence, its cotranslational nascent-chain elongation rate, and foldi

163 We propose a model for cotranslational oxidative folding wherein PDI acts as a

164 targeting polypeptides for secretion via the cotranslational pathway.

165 ttranslational pathway and the SRP-dependent cotranslational pathway.

166 llosteric agonist NPS R-568, which acts as a cotranslational pharmacochaperone.

167 Cotranslational presence of MTP can dramatically promote

168 The cotranslational, primary self-cleavage reaction of cardi

169 nto the membrane does not occur via a simple cotranslational process.

170 occurs as a post-translational rather than a cotranslational process.

171 approaches have been combined to understand cotranslational processes at multiple scales, and the ne

172 this review, we discuss recent insights into cotranslational processes gained from molecular simulati

173 Cotranslational processes involving a nascent protein in

174 anifestation of the nonequilibrium nature of cotranslational processes, and as such, there exist theo

175 nection between the nonequilibrium nature of cotranslational processes, nascent-protein behavior, and

176 ation of a multiple exit system facilitating cotranslational processing (or folding or directing) of

177 translation of the NFKB1 gene and that this cotranslational processing allows the production of both

178 products identical to those observed during cotranslational processing of the authentic ORF1 polypro

179 As a consequence, different cotranslational profiles of the same protein can have di

180 They facilitate cotranslational protein complex formation, which establi

181 This process, called cotranslational protein degradation (CTPD), has been obs

182 previously unknown role for Srp1 and Sts1 in cotranslational protein degradation and suggests a novel

183 ich technique was used to detect and measure cotranslational protein degradation in living cells.

184 We further demonstrate that cotranslational protein degradation is generally impaire

185 Cotranslational protein degradation plays an important r

186 How cotranslational protein folding and the rate of synthesi

187 function to regulate protein translation and cotranslational protein folding and to target and degrad

188 ict that fast-translating codons can enhance cotranslational protein folding by helping to avoid misf

189 along a transcript, have been shown to alter cotranslational protein folding, suggesting that evoluti

190 translated can increase their probability of cotranslational protein folding, while speeding up codon

191 h interfering with translation kinetics, and cotranslational protein folding.

192 olypeptide chain elongation that accompanies cotranslational protein folding.

193 age "code" within genetic codons to regulate cotranslational protein folding.

194 rotein biosynthesis is inherently coupled to cotranslational protein folding.

195 t as slow-translating codons in coordinating cotranslational protein folding.

196 During cotranslational protein integration into the ER membrane

197 Cotranslational protein maturation is often studied in c

198 N-Linked glycosylation is a common cotranslational protein modification that can regulate p

199 hydrolysis at the primary cleavage site, or cotranslational protein modifications that expose an oth

200 esent results indicate that mTORC2-catalyzed cotranslational protein phosphorylation is a core functi

201 ses Hsf1 monitors and elucidates a conserved cotranslational protein quality control mechanism.

202 cribes how these features may play a role in cotranslational protein quality control.

203 ns, eukaryotic release factors contribute to cotranslational protein quality control.

204 20-amino-acid leader sequence that permitted cotranslational protein synthesis of IT along with trunc

205 During cotranslational protein targeting by the signal recognit

206 An example of such control occurs in cotranslational protein targeting by the signal recognit

207 Cotranslational protein targeting by the signal recognit

208 Cotranslational protein targeting delivers proteins to t

209 Cotranslational protein targeting to membranes is regula

210 ptor (FtsY in prokaryotes) are essential for cotranslational protein targeting to the endoplasmic ret

211 During cotranslational protein targeting, two guanosine triphos

212 ves as docking sites for both TF and SRP, in cotranslational protein targeting.

213 n particle (SRP) is universally required for cotranslational protein targeting.

214 (SRP) plays an integral role in Sec-mediated cotranslational protein translocation and membrane prote

215 al time for recognition by the machinery for cotranslational protein translocation into the ER.

216 Cotranslational protein translocation is a universally c

217 P is required for viability, suggesting that cotranslational protein translocation is an essential pr

218 broad outer-membrane defects and premature, cotranslational protein translocation.

219 Cotranslational protein transport to the endoplasmic ret

220 These findings are suggestive of a role for cotranslational protein-processing pathways in maintaini

221 xtent to which nascent chains are subject to cotranslational quality control and degradation remains

222 Therefore, cells evolved cotranslational quality control mechanisms that eliminat

223 hese results suggest that CaSR is subject to cotranslational quality control, which includes a pharma

224 chanisms act in concert to promote efficient cotranslational recruitment.

225 Moreover, excess TF interferes with cotranslational removal of the N-terminal formyl methion

226 Their cotranslational rerouting to the cytosol for degradation

227 bosome stalling on eukaryotic mRNAs triggers cotranslational RNA and protein degradation through cons

228 strate the global prevalence and features of cotranslational RNA decay in a plant transcriptome.

229 nalysis in xrn4 mutant plants indicates that cotranslational RNA decay is XRN4 dependent.

230 iple protein kinases, perhaps by virtue of a cotranslational role in protein folding.

231 ated by a viral peptide sequence that causes cotranslational scission of emerging peptide chains.

232 we present an engineered system for specific cotranslational Sep incorporation (directed by UAG) into

233 present a strategy called folding-associated cotranslational sequencing that enables monitoring of th

234 We also apply folding-associated cotranslational sequencing to track cotranslational fold

235 ed to the periplasm when it is attached to a cotranslational signal sequence.

236 oplasmic reticulum via an alternative to the cotranslational, signal recognition particle-dependent m

237 relevant to cpSRP54's role in the post- and cotranslational signaling processes.

238 aminoacyl-tRNA synthetase/tRNA pair for the cotranslational, site-specific incorporation of two unna

239 The mechanism of targeting relies on cotranslational SRP binding to hydrophobic signal sequen

240 Here, we report the dispensability of the cotranslational SRP protein translocation system in a ba

241 "2A-like" sequence element that specifies a cotranslational "stop/continue" event releasing the exog

242 The early elongation pausing represents a cotranslational stress response to maintain the intracel

243 sensitive and global approach to define the cotranslational substrate specificity of the yeast Hsp70

244 lves spatially confined, actively chaperoned cotranslational subunit interactions.

245 t presentation of the epitope depended on 1) cotranslational synthesis of E1 in the ER, 2) glycosylat

246 Here, we report an approach to the cotranslational synthesis of selectively glycosylated pr

247 C-terminus, underscores the contribution of cotranslational synthesis to TMD inversion.

248 lly thought to occur via two major pathways: cotranslational targeting by signal recognition particle

249 perone trigger factor (TF) and the essential cotranslational targeting machinery, signal recognition

250 vivo evidence that there is an SRP-dependent cotranslational targeting mechanism in E. coli and argue

251 Our results demonstrate that cotranslational targeting of a protein to the E. coli Se

252 ed protein targeting machine and mediate the cotranslational targeting of inner membrane proteins to

253 Cotranslational targeting of membrane proteins is mediat

254 y is achieved in one of these processes, the cotranslational targeting of nascent membrane and secret

255 icle (SRP) and SRP receptor (SR) mediate the cotranslational targeting of proteins to cellular membra

256 e major cellular machinery that mediates the cotranslational targeting of proteins to cellular membra

257 otein, is known to mediate the GTP dependent cotranslational targeting of proteins to the endoplasmic

258 g signal recognition particle (SRP)-mediated cotranslational targeting of proteins to the membrane.

259 on of Sbh1p and Sbh2p causes a defect in the cotranslational targeting pathway that is similar to the

260 y mimicking the effect of the SRP RNA in the cotranslational targeting pathway.

261 the N-terminal extension, however, restored cotranslational targeting.

262 ligosaccharyltransferase (OST) catalyzes the cotranslational transfer of high-mannose sugars to nasce

263 sting data and our findings, we propose that cotranslational translocation and N-glycosylation of nas

264 nt opsin peptides have a lower efficiency of cotranslational translocation but an even lower efficien

265 Surprisingly, the slow growth and cotranslational translocation defects caused by deletion

266 Cotranslational translocation defects in the ssh1DeltaSR

267 marine cyanobacteria that act by preventing cotranslational translocation early in the secretory pat

268 We propose a binary model of cotranslational translocation entailing only two basic f

269 hat initial protein topology established via cotranslational translocation events in the ER is dynami

270 QP4 acquires each of its six TM segments via cotranslational translocation events, whereas AQP1 is in

271 slocon component associations during defined cotranslational translocation events.

272 Signal sequence recognition in cotranslational translocation in mammals appears to occu

273 he RQC pathway can also target stalls during cotranslational translocation into the ER is not known.

274 nd raises the possibility that inhibition of cotranslational translocation may be exploited for antic

275 ecludes them from utilizing the well-studied cotranslational translocation mechanism of most transmem

276 Cotranslational translocation of apoB100 across the endo

277 These results are consistent with cotranslational translocation of env into the ER, where

278 s, we demonstrated that apratoxin A prevents cotranslational translocation of proteins destined for t

279 Cotranslational translocation of proteins requires ribos

280 the DsbA signal sequence promotes efficient, cotranslational translocation of the cytoplasmic protein

281 erates with either a translating ribosome in cotranslational translocation or the SecA ATPase in post

282 ocation of nascent polypeptides that use the cotranslational translocation pathway.

283 sec61 mutants affect different steps in the cotranslational translocation pathway.

284 ve defects in both the posttranslational and cotranslational translocation pathways due to a kinetic

285 e revealed that modulation of Sec61-mediated cotranslational translocation selectively impaired glyco

286 Cotranslational translocation was expected to be ineffic

287 ast, point mutations in L6 of Sec61p inhibit cotranslational translocation without significantly redu

288 the nascent HER3 protein from initiating its cotranslational translocation, resulting in the degradat

289 In cotranslational translocation, the ribosome feeds the po

290 hain domains not only before but also during cotranslational translocation.

291 ration into a cyclodepsipeptide inhibitor of cotranslational translocation.

292 ese predictions using an assay for cell-free cotranslational translocation.

293 These data show that cotranslational translocon contacts are ultimately contr

294 tially implying a new layer of regulation of cotranslational transport.

295 We report that cotranslational ubiquitination (CTU) is a robust process

296 Here, we directly and quantitatively assess cotranslational ubiquitination and identify, at a system

297 Cotranslational ubiquitination occurs at very low levels

298 translational folding remain susceptible for cotranslational ubiquitination.

299 ational degradation of nascent polypeptides, cotranslational ubiquitylation occurs at a low level, su

300 reaction and that the mode of translocation (cotranslational verse posttranslational) can affect the