ライフサイエンスコーパス: counter selection

1 are isolated directly using sucrose-mediated counter-selection.

2 ences to be removed from the library through counter-selection.

3 Through puromycin selection and ganciclovir counter-selection, a targeting efficiency of over 50% wa

4 mut expression is maintained in vivo with no counter selection against reporter-labeled cells.

5 B is orders of magnitude more sensitive as a counter-selection agent than either gene alone.

6 rove gene targeting in C. elegans, we used a counter-selection approach to reduce the number of false

7 requency by 1.3E6-fold in total, making tolC counter-selection as effective as most selectable marker

8 cular, it resulted in more B cells surviving counter-selection at the transitional stages.

9 h a polymorphism might be maintained despite counter-selection by malaria, we have carried out functi

10 We fundamentally improved the tolC counter-selection by supplementing a second agent, vanco

11 ulted in many rearranged clones in which the counter-selection cassette had been deleted.

12 ng a second agent, vancomycin, which reduces counter-selection escape by 425-fold, compared colicin E

13 a mismatch repair proficient strain reduced counter-selection escape frequency by 1.3E6-fold in tota

14 ight and guided strain engineering to reduce counter-selection escape frequency by approximately 40-f

15 a selectable/counter-selectable marker, but counter-selection escape frequency using colicin E1 prec

16 encing of 96 independent lineages exhibiting counter-selection escape to identify loss-of-function mu

17 efits to more-cooperative individuals, which counter selection for cheating; however, empirical evide

18 costs of virulence to be the main mechanism countering selection for pathogen complexity, many other

19 nvaluable for genetic manipulation, although counter-selection has historically exhibited limited rob

20 volving the URA3/5-fluoroorotic acid (5-FOA) counter-selection have shown a high background of 5-FOA

21 1 generations, thereby eliminating potential counter selection in females.

22 , and suggest a rationale for their apparent counter selection in RNA processing sites.

23 benefiting one sex may not accumulate due to counter selection in the other sex.

24 The two-step process of selection and counter-selection is a standard way to enable genetic mo

25 as a positive-selection marker and GFP as a counter-selection marker which is lost during homologous

26 Additionally, there are few well developed counter-selection markers for use in Burkholderia.

27 ome the limited availability of heterologous counter-selection markers, here we explore novel DNA int

28 The use of SacB as a counter-selection method has been of limited success due

29 such as BCL-XL is thought to result from the counter selection of sensitive, low expresser clones dur

30 ction of single-crossover clones followed by counter-selection of double-crossover clones.

31 mplications in both repertoire selection and counter-selection of premalignant clones for leukemia su

32 stance, while plasmid excision is secured by counter-selection of the pIJ2581 glkA gene, which confer

33 recombineering coupled to a robust two-step counter-selection protocol.

34 We are applying counter-selection recombineering to modify bacmid bMON14

35 here should prove useful to others applying counter-selection recombineering to modify BACs or PACs

36 seamless modifications can be achieved using counter-selection strategies in which a donor cassette c

37 We have used a counter-selection strategy based on aberrant phytochrome

38 ed in a DNA cassette and confer a novel dual counter-selection system.

39 me that cannot be directly detected by other counter-selection systems.

40 rcumvents the need for selectable markers or counter-selection systems.

41 on in all isolates, despite the absence of a counter-selection to remove FAD-binding RNAs.

42 rm stable and continuous rounds of selection/counter-selection using tolC, enabling replacement of 10

43 First, a URA3 counter selection was adapted to link chemical dimerizer

44 A genetic system based on rpsL-streptomycin counter selection was developed to further dissect the m

45 Next, the URA3 counter selection was shown to detect cellulase activity

46 of 1264 and BEAS cells used for panning and counter-selection was estimated as 75,000 +/- 5,000 and