ライフサイエンスコーパス: covalent attachment

1 This GAF domain by itself is sufficient for covalent attachment.

2 >1 h after removal of drug presumably via a covalent attachment.

3 he top of the E helix using a site-specific, covalent attachment.

4 si-face of the flavin ring, near the site of covalent attachment.

5 eties positioned strategically to facilitate covalent attachment.

6 -avidin chemistry, as well as through direct covalent attachment.

7 ntigen) self-assembled onto Au-electrode via covalent attachment.

8 ur results support D35 as being the point of covalent attachment.

9 he sulfenyl amide residue to generate stable covalent attachments.

10 avoids loss of biological activity caused by covalent attachment; (2) we constructed a well-blocked s

11 portant in adhesion, implying a mechanism of covalent attachment analogous to that used by human comp

12 e next identified residues important for the covalent attachment and selected the FrdA(E245) residue,

13 polymer multilayers, and permits the direct covalent attachment and subsequent patterning of chemica

14 This investigation utilized the covalent attachment approach to identify receptor residu

15 lly biologically active probes with sites of covalent attachment at positions 28 and 31.

16 lized by an additional 2.4 kcal/mol by their covalent attachment at wildtype GPDH.

17 tubes (CNTs) via three different preparation covalent attachment (CA), enzyme coating (EC) and enzyme

18 Ts) via three different preparation methods: covalent attachment (CA), enzyme coating (EC) and enzyme

19 We also show that covalent attachment has no effect on the catalytic prope

20 l ligand to the heme iron in preparation for covalent attachment; (ii) to spatially position the two

21 tin-like photolabile probes having sites for covalent attachment in positions 21 and 23 and used sequ

22 ist photolabile GLP1 probes having sites for covalent attachment in positions 24 and 35.

23 nist photolabile GLP1 probes having sites of covalent attachment in positions 6 and 12 of the 30-resi

24 tuent, thus providing an inhibitor with dual covalent attachment in the enzyme active site.

25 hotolabile benzoylphenylalanines as sites of covalent attachment, in positions known to label distinc

26 No covalent attachment is observed in the absence of campho

27 trategies on stiff, glass substrates and non-covalent attachment methods are limiting.

28 s of a functional protein microarray such as covalent attachment, native protein conformation, homoge

29 netii, we found that four different types of covalent attachments occur between OM proteins and PG, w

30 nocomposite was applied as the interface for covalent attachment of 3'-amine-terminated capture probe

31 This requires bridging receptor subunits via covalent attachment of 4,4'-bis(maleimido)azobenzene to

32 lectroreduction of graphene oxide to RGO and covalent attachment of 4-ABA are achieved by applying al

33 4(OPr(i))20 nanoparticles, functionalized by covalent attachment of 4-nitrophenyl-acetylacetonate or

34 rescence microscopy to monitor the localized covalent attachment of 5- (and 6)-carboxytetramethylrhod

35 al peptide biosynthetic pathways, catalyzing covalent attachment of a 4'-phosphopantetheinyl group to

36 Thus, the covalent attachment of a benzophenone or a naphthalene t

37 Protein N-myristoylation occurs by a covalent attachment of a C14:0 fatty acid to the N-termi

38 We report here covalent attachment of a catalytically active cobalt com

39 Covalent attachment of a cell-penetrating peptide helps

40 ystallographic analyses demonstrate that the covalent attachment of a cyanide anion to the indoline f

41 s also modified with a silica shell to allow covalent attachment of a cyclic RGD peptide and an organ

42 We conjecture that covalent attachment of a hydroxymethylene, carboxy, or a

43 he closed channel and that this is broken by covalent attachment of a larger lipophilic moiety at the

44 uding Ras GTPase family members, require the covalent attachment of a lipid group by protein farnesyl

45 human serum albumin conjugate through stable covalent attachment of a maleimido-C34 analog onto cyste

46 Previous work demonstrated that covalent attachment of a metal complex, Ru(II)(bpy)3, gr

47 eudocontact shifts, which are invoked by the covalent attachment of a paramagnetic lanthanoid chelati

48 s determined that AnkX directly mediates the covalent attachment of a phosphocholine moiety to Rab1.

49 on the suppression of PI resistance using a covalent attachment of a phosphonic acid motif to a pept

50 l oxidation, following photosensitization by covalent attachment of a photo-oxidant at position 355[b

51 fficient amounts of primary amine groups for covalent attachment of a polyethylene glycol antifouling

52 Although the covalent attachment of a polyubiquitin is the prevailing

53 unctionality is demonstrated by simultaneous covalent attachment of a rhodamine FRET acceptor and bin

54 ntroduced at 12 positions in hSERT to enable covalent attachment of a rhodamine-based fluorophore.

55 cytochrome P450-BM3 (CYP102A1) has required covalent attachment of a Ru(II) photosensitizer to a non

56 The covalent attachment of a shell of oligonucleotides to th

57 One such modification, the covalent attachment of a single isoprenoid lipid (prenyl

58 osttranslational modification of proteins by covalent attachment of a small protein ubiquitin (Ub) or

59 at FOXA2 is post-translationally modified by covalent attachment of a small ubiquitin related modifie

60 We then imparted light sensitivity through covalent attachment of a synthetic glutamate-based photo

61 Covalent attachment of a synthetic triantennary N-acetyl

62 This chemistry involved the covalent attachment of a thiol-functionalized silane anc

63 Drosophila Fic (dFic) mediates AMPylation, a covalent attachment of adenosine monophosphate (AMP) fro

64 ity of the VHH scaffold and sortase-mediated covalent attachment of Ags suggests their broader applic

65 To obtain evidence for the site of covalent attachment of AM841, C6.47, identified in part

66 s consisting of carboxylic groups that allow covalent attachment of amine-terminated oligonucleotide

67 nalized pCB is shown to be recovered only by covalent attachment of amino acid deactivation agents to

68 Aminoacyl-tRNA synthetases catalyze the covalent attachment of amino acids onto their cognate tR

69 s are multidomain proteins that catalyze the covalent attachment of amino acids to their cognate tran

70 wire in between gold electrodes, followed by covalent attachment of amino-modified probe oligonucleot

71 rface acted as a template for the subsequent covalent attachment of aminohexyl-modified DNA oligonucl

72 Solubilization of membrane proteins by covalent attachment of amphiphiles results in homogeneou

73 w, and general method for the reversible non-covalent attachment of amphiphilic DNA probes containing

74 attributed to nonuniform charge arising from covalent attachment of ampholytes to the benzophenone-fu

75 In this work, the covalent attachment of an amine functionalized metal-org

76 contribution, we describe the synthesis and covalent attachment of an analogue of the flavin mononuc

77 This is one of the first examples where the covalent attachment of an ionophore drastically improves

78 er of NO bioactivity is S-nitrosylation, the covalent attachment of an NO moiety to a protein cystein

79 2) selectivity and to enhance drug safety by covalent attachment of an organic nitrate moiety as a ni

80 We report the straightforward oriented covalent attachment of antibodies (Abs) on the surface o

81 Covalent attachment of antibodies on 2D PC membrane base

82 The surfaces were functionalized by the covalent attachment of antibodies, streptavidin, and oli

83 anedithiol to facilitate membrane fusion and covalent attachment of antibodies.

84 e structural basis of KLK7 inhibition by the covalent attachment of aromatic coumarinic esters.

85 matic electrode is based on the step-by-step covalent attachment of AuNPs and Lc molecules to porous

86 um groups with ERGO and subjected to further covalent attachment of Azure A (Azu-A) mediator or gluco

87 Zn(2+) binding in an engineered site and the covalent attachment of benzocaine-methanethiosulfonate t

88 The activation of surfaces by covalent attachment of bioactive moieties is an importan

89 ated into the framework to provide sites for covalent attachment of biologically relevant cargoes whi

90 Covalent attachment of biorecognizable sugar ligands in

91 The BirA-catalyzed reaction involves the covalent attachment of biotin to AccB, a subunit of acet

92 irA catalyzes the adenylation and subsequent covalent attachment of biotin to the biotin carboxyl car

93 Ligation results in covalent attachment of biotin to the microplate and prov

94 u NPs) are decorated on the GO layer for the covalent attachment of biotin, which is then used to sel

95 ger, cucumber, milk, and lettuce) even after covalent attachment of BREs to carboxy groups of CBMAA.

96 ates a stable thioester bond, leading to the covalent attachment of C3b to cell-surface or protein-su

97 robe and PAMAM-Dendrimers/AuNPs was used for covalent attachment of CA125-antibody and completing the

98 e-linked glycosylation, the co-translational covalent attachment of carbohydrates to asparagine side

99 combining this stereoelectronic effect with covalent attachment of carboxylate as a base, aniline ra

100 vel adhesion layer is described that enables covalent attachment of cell adherent molecules to the su

101 The reversible covalent attachment of chemical probes to proteins has l

102 eveloped HNO-sensing electrode, based on the covalent attachment of cobalt(II) 5,10,15,20-tetrakis[3-

103 chemical technique since it leads to direct covalent attachment of conjugated alkynes to silicon.

104 rastically enhance the ECL is established by covalent attachment of coreactants N,N-diethylethylenedi

105 s-linked CpaN dimer provides a model for the covalent attachment of Cpa to target receptors and thus

106 ngle-atom catalyst, Cu/UiO-66, prepared by a covalent attachment of Cu atoms to the defect sites at t

107 This body of work suggests that the covalent attachment of cucurbit[n]uril to metal organic

108 x in a precise 1:1 stoichiometry followed by covalent attachment of Cy5-Trolox conjugates onto dendri

109 describe a novel method that integrates the covalent attachment of DNA handles to target proteins wi

110 RNA content by an order of magnitude through covalent attachment of each strand of the duplex.

111 The covalent attachment of electron deficient perfluoroaryl

112 accessible upon pocket opening for selective covalent attachment of electrophilic ligands in eubacter

113 parent SBA-15 materials and allow efficient covalent attachment of ethynylated organic moieties thro

114 uccinate dehydrogenase (SDH) is dependent on covalent attachment of FAD on the ~70-kDa flavoprotein s

115 homologs, but the mechanisms underlying the covalent attachment of FAD remain to be fully elucidated

116 d in reaction catalysis and in mediating the covalent attachment of FAD, whereas Tyr-448 serves as an

117 l modification is S-acylation, involving the covalent attachment of fatty acids to cysteine residues

118 tochondrial protein Sdh5 is required for the covalent attachment of flavin adenine dinucleotide (FAD)

119 of the channel is faithfully mimicked by the covalent attachment of fluorescein at a cysteine residue

120 Covalent attachment of functional groups is generally ac

121 PTMs involve the covalent attachment of functional groups to specific ami

122 site of GFP-like proteins, and may allow for covalent attachment of functional groups to the protein

123 Covalent attachment of GlcNAc to the HAT domain of long

124 n integrated modified electrode based on the covalent attachment of glucose dehydrogenase (GDH) enzym

125 hibits the activity of SidEs by inducing the covalent attachment of glutamate moieties to SdeA-a memb

126 In fact, the covalent attachment of graphene quantum dots with N-acry

127 lso allow layer-specific postcrystallization covalent attachment of guest molecules.

128 A hallmark of our assembly strategy is the covalent attachment of guideRNA and editing enzyme by ap

129 All models propose that CcmFH facilitates covalent attachment of haem to the apocytochrome; namely

130 HA can be modified by the covalent attachment of heavy chains that are derived fro

131 chondrial cytochrome c assembly requires the covalent attachment of heme by thioether bonds between h

132 The covalent attachment of heme cofactors to the apo-polypep

133 The c-type cytochromes, defined by the covalent attachment of heme to a CXXCH motif, are key el

134 c-type cytochromes, which are defined by the covalent attachment of heme to a CXXCH motif.

135 ), a mitochondrial enzyme that catalyzes the covalent attachment of heme to c-type cytochromes.

136 The covalent attachment of IFN-beta and NAg was not necessar

137 polyphosphorylation (K-PPn), consists of the covalent attachment of inorganic polyphosphate (polyP) t

138 we characterize "polyphosphorylation" as the covalent attachment of inorganic polyphosphate (polyP) t

139 be discerned, thereby demonstrating a robust covalent attachment of intact clusters to the graphene s

140 Post-translational modification by covalent attachment of isoprenoid lipids (prenylation) r

141 ct with the C-terminal ester of 3, forming a covalent attachment of L-Lys-D-Ala to the natural produc

142 ctivating mitochondrial proteins through the covalent attachment of lipoate (lipoylation).

143 two well defined pathways for synthesis and covalent attachment of lipoic acid in prokaryotes.

144 SMFS became possible by the covalent attachment of long and defined single polymers

145 merization chemistry, thereby leading to the covalent attachment of longer graft copolymer chains to

146 cus aureus transpeptidase SrtA catalyzes the covalent attachment of LPXTG-containing virulence and co

147 Cleavage resulted in covalent attachment of MBP-VirD2 to the 5'-cleaved end,

148 The covalent attachment of molecules is demonstrated by appe

149 ey step in FA-BRCA pathway activation is the covalent attachment of monoubiquitin to FANCD2 and FANCI

150 Interaction with Tsg101 is strengthened by covalent attachment of monovalent ubiquitin to HIV-1 p6.

151 residues 1-10 comprise a consensus motif for covalent attachment of myristate, a hydrophobic 14-carbo

152 N-myristoylation is the covalent attachment of myristic acid to the N terminus o

153 ns are posttranslationally modified with the covalent attachment of N-glycans.

154 Correct folding of ER proteins requires covalent attachment of N-linked glycan oligosaccharides.

155 llin-type proteins has been shown to require covalent attachment of Nedd8 (neural precursor cell-expr

156 sponses is through S-nitrosylation, that is, covalent attachment of NO to cysteine residues to form S

157 Glycosylation is a stepwise procedure of covalent attachment of oligosaccharide chains to protein

158 Covalent attachment of palmitate to the N-terminal cyste

159 The covalent attachment of PARP-1 to AP site-containing DNA

160 ted cross-linking reactions resulting in the covalent attachment of pathogenic Staphylococcus aureus

161 w that both CpcS-I and CpcU are required for covalent attachment of PCB to Cys-82 of the PC beta subu

162 IRT2, a tryptic peptide originating from the covalent attachment of photoactivated 4 was identified.

163 Covalent attachment of polyethylene glycol (M(n) = 2 x 1

164 The covalent attachment of polysaccharide antigens to the ca

165 The covalent attachment of porphyrins to DNA is therefore a

166 ormin that binds actin monomers directly via covalent attachment of profilin to its binding site in t

167 in a process referred to as pupylation, the covalent attachment of prokaryotic ubiquitin-like protei

168 operties of the conducting pathway opened by covalent attachment of propyl-MTS were the same as those

169 amine-poly(ethylene glycol)-carboxyl allowed covalent attachment of proteins to the particles using s

170 two different TTR constructs, each featuring covalent attachment of proximal subunits.

171 that the critical factor may be the lack of covalent attachment of PS to protein in intact Pn, highl

172 old nanoparticles (AuNPs-PAMAM) was used for covalent attachment of PSA-aptamer and HRP linked aptame

173 Covalent attachment of PSII in its electrostatically imp

174 rufin ligase catalyzed the site-specific and covalent attachment of resorufin to various cellular pro

175 Covalent attachment of sensing and reference membranes t

176 istries for interprotofibril association and covalent attachment of serpins that regulate clot dissol

177 ttranslational modification characterized by covalent attachment of small ubiquitin-like modifier (SU

178 The covalent attachment of small ubiquitin-like modifier (SU

179 nucleic acids at specific sites requires the covalent attachment of spin labels, which involves rathe

180 4-mercaptobenzoic acid onto a gold surface, covalent attachment of streptavidin and further immobili

181 High-affinity labeling is demonstrated by covalent attachment of streptavidin, thus enabling the t

182 O-Linked glycosylation often involves the covalent attachment of sugar moieties to the hydroxyl gr

183 The covalent attachment of SUMO (small ubiquitin-like modifi

184 ve to SUMO-1 overexpression, suggesting that covalent attachment of SUMO-1 to p6 is detrimental to HI

185 eptor that allows for the post-translational covalent attachment of targeting ligands at the T-cell s

186 ipass transmembrane enzyme that mediates the covalent attachment of the 16-carbon fatty acid palmitat

187 o-ACPs) are post-translationally modified by covalent attachment of the 4'-phosphopantetheine group t

188 The covalent attachment of the 76 amino acid peptide ubiquit

189 bable nucleophile, thus acting as a site for covalent attachment of the acyl molecule and formation o

190 roxysuccinimide ester groups resulted in the covalent attachment of the alkene as a monolayer on the

191 d unprecedented reaction mechanism involving covalent attachment of the alpha,beta-unsaturated aldehy

192 ing surface served as a stable layer for the covalent attachment of the anti-human CRP antibody.

193 ross-linkers around the AuNP followed by the covalent attachment of the antibodies.

194 However, efficient covalent attachment of the completed pilus structure to

195 sis and rigorous control experiments confirm covalent attachment of the designed ligand and its resul

196 ofurane-co-arylacetic acid), followed by the covalent attachment of the enzyme and embedment of the p

197 otent severing activity that is dependent on covalent attachment of the FH2 to the C terminus.

198 and adjusts the pK(a) of SDHA(R451) so that covalent attachment of the flavin adenine dinucleotide (

199 Covalent attachment of the fluorescence-quenching 14-mer

200 uration (Ccm) system is known to promote the covalent attachment of the haem to periplasmic apocytoch

201 igestion of the G248E P450(cam) mutant after covalent attachment of the heme releases 5-hydroxyheme,

202 The covalent attachment of the hybrid POM forms new nanocomp

203 Covalent attachment of the hydrogel to the substrate was

204 The covalent attachment of the ionophore to the polymer matr

205 This dimer formation is eliminated here by covalent attachment of the ionophore to the polymer matr

206 terized by a longer duration consistent with covalent attachment of the ligand to functionally import

207 rgo S-palmitoylation, the post-translational covalent attachment of the long chain fatty acid palmita

208 Covalent attachment of the N-glycosylated BfrA onto the

209 Ls contain a reactive electrophile, enabling covalent attachment of the photoswitch to naturally occu

210 Ubiquitylation and sumoylation, the covalent attachment of the polypeptides ubiquitin and SU

211 The covalent attachment of the porphyrin to the polypeptide

212 f its amino-terminal domain as the region of covalent attachment of the position 12 probe, whereas th

213 een used as the substrates of PPTase for the covalent attachment of the probes to a specific Ser resi

214 By covalent attachment of the redox reporter molecules on t

215 Covalent attachment of the small ubiquitin-like modifier

216 n product is a thiol-cyanine adduct in which covalent attachment of the thiol to the polymethine brid

217 nilino-8 naphthalene sulfonate (ANS) and the covalent attachment of the thiol-reactive fluorophore, m

218 Ubiquitination involves the covalent attachment of the ubiquitin (Ub) C-terminus to

219 quitin ligase, which becomes functional upon covalent attachment of the ubiquitin homologue neural-pr

220 E3 ubiquitin ligases are regulated by the covalent attachment of the ubiquitin-like protein Nedd8

221 The covalent attachment of the ubiquitin-like protein Nedd8

222 Covalent attachment of the ubiquitin-like protein NEDD8

223 alogous to ubiquitination that culminates in covalent attachment of the ubiquitin-like protein Nedd8

224 ography (UHPLC) applications was prepared by covalent attachment of the Whelk-O1 selector to spherica

225 but these polymers lack reactive groups for covalent attachment of therapeutic molecules.

226 nds of the bundle facilitates the subsequent covalent attachment of these monolayer ensembles onto th

227 le-cysteine mutations that allowed selective covalent attachment of thiol-reactive dyes.

228 E2s (UbcH6 and UBE2E2) uniquely requires the covalent attachment of Ub to the active site cysteine of

229 me system in which substrates, marked by the covalent attachment of Ub, are degraded by the 26 S prot

230 The covalent attachment of ubiquitin (Ub) to various intrace

231 Post-translational covalent attachment of ubiquitin and ubiquitin-like prot

232 In eukaryotes, the covalent attachment of ubiquitin chains directs substrat

233 The covalent attachment of ubiquitin may "lock" p53 in a mut

234 Ubiquitination, the covalent attachment of ubiquitin molecules to proteins,

235 st-translational modification of proteins by covalent attachment of ubiquitin or a polyubiquitin chai

236 nal modification of cellular proteins by the covalent attachment of ubiquitin regulates protein stabi

237 t-translational modification that involves a covalent attachment of ubiquitin to a protein substrate,

238 Covalent attachment of ubiquitin to a substrate is catal

239 Covalent attachment of ubiquitin to proteins regulates a

240 between-RING E3 ligase that functions in the covalent attachment of ubiquitin to specific substrates,

241 E3 ligases mediate the covalent attachment of ubiquitin to target proteins ther

242 omplex enzymatic process that results in the covalent attachment of ubiquitin, through Gly-76 of ubiq

243 The successful covalent attachment of up to 11 tetraphenyl porphyrins i

244 s a chemical surface modification method for covalent attachment of various polymers by using silane-

245 ortase transpeptidase is responsible for the covalent attachment of virulence- and colonization-assoc

246 ion rates were dependent on both the site of covalent attachment on the DNA and the size of the bound

247 uation in the cluster prove the analogy to a covalent attachment on TMDCs.

248 the listeria bacteriophage endolysin Ply500: covalent attachment onto FDA approved silica nanoparticl

249 ed on the surface of live cells using either covalent attachment or non-covalent insertion, while mai

250 presentation of relevant carbohydrates using covalent attachment or supramolecular self-assemblies.

251 ectrode-electrolyte interface either through covalent attachment or surface adsorption, enabling the

252 ational studies have provided details of the covalent attachment process that leads to formation of t

253 troscopic mapping of the distribution of the covalent attachment revealed that activated 4-methoxyphe

254 alysis, we are able to define a single AzBCQ covalent attachment site lying within the digestive vacu

255 tween the N-terminal segment, containing the covalent attachment site of the chromophore, and the PHY

256 The antagonist covalent attachment site to the receptor served as a gui

257 of thiol-ene chemistry allows for a range of covalent attachment strategies in a fast and reliable ma

258 Proteins are immobilized via covalent attachment to a channel-filling benzophenone-fu

259 on the gold surface of an SPR sensor through covalent attachment to a self-assembled monolayer (SAM)

260 ognition groups are forced into proximity by covalent attachment to a single lipid headgroup.

261 This has previously been achieved by covalent attachment to a variety of cell-penetrating pep

262 inding affinity increases significantly upon covalent attachment to ACB.

263 s circuit conductance to monitor and control covalent attachment to an electrically connected SWNT.

264 functionalized with carboxylic acids enable covalent attachment to bead and silica supports.

265 form stable radiocopper complexes and allow covalent attachment to biological molecules.

266 Here we provide evidence that covalent attachment to carrier proteins is not required

267 of biosynthetic intermediates, displayed via covalent attachment to carrier proteins, by catalytic do

268 d PV3) with various anchoring groups for the covalent attachment to Co(3)O(4) catalyst nanoparticles

269 ents can be rendered membrane impermeable by covalent attachment to cobalamin (Cbl) through a photocl

270 to the cytochrome c synthetase (CcmF/H) for covalent attachment to cytochrome c by unknown mechanism

271 es reactive metabolites, they are trapped by covalent attachment to DNA bases.

272 e catalytic tyrosine residue responsible for covalent attachment to DNA is located in a smaller subun

273 and isophorone diisocyanate was performed by covalent attachment to free isocyanate groups from PUU m

274 late carriers (Sepabeads EC-EA and EC-HA) or covalent attachment to glyoxal agarose.

275 lly modified phospholipid anchors capable of covalent attachment to O(6)-methylguanine DNA methyltran

276 Through their covalent attachment to peptidoglycan via an arabinogalac

277 ate diverse cellular processes through their covalent attachment to target proteins.

278 e cellular processes through its reversible, covalent attachment to target proteins.

279 s the orientation of the inactivator and its covalent attachment to the active site Cys, but the stru

280 teins relies on an enzyme called sortase for covalent attachment to the cell wall.

281 y Gram-positive bacteria is sortase-mediated covalent attachment to the cell wall.

282 ins we show the SPKTG motif is essential for covalent attachment to the cell wall.

283 comes significantly less efficient after the covalent attachment to the luminescent partner.

284 phy revealed that both inhibitors bind L2 by covalent attachment to the nucleophilic serine.

285 abolic use as a cellular nutrient as well as covalent attachment to the pigment.

286 iguration of the chromophore and its mode of covalent attachment to the protein.

287 trifluoromethylacetamide derivatives without covalent attachment to the protein.

288 ing the DNA strand to be transferred and for covalent attachment to the resulting 5'-phosphate end, a

289 Y and MpeZ controls differential PEB and PUB covalent attachment to the same cysteine residue.

290 ction of this N-domain is not constrained to covalent attachment to Vam7p.

291 We used covalent attachments to single-walled carbon nanotubes (

292 Covalent attachments to the stimulator were also identif

293 y (e.g., electrostatic, hydrogen bonding, or covalent attachment) to attach scFv to preformed functio

294 ed on the porous iridium oxide electrodes by covalent attachment using (3-aminopropyl)triethoxysilane

295 uired for positioning the first cysteine for covalent attachment, whereas the histidine of CXXCH posi

296 the subsequent single strand of DNA (ssDNA) covalent attachment, which after hybridization with its

297 obe must approach the target biomolecule for covalent attachment, while avoiding irreversible adhesio

298 bind irreversibly to WT CB1 but exhibited no covalent attachment with the mutants and behaved as an a

299 e azido groups, both of which are capable of covalent attachment with the target protein.

300 the DNA substrates whilst forming transient covalent attachments with the recessed 5' ends.