ライフサイエンスコーパス: cross peak

1 ly, and significant narrowing of the His-258 cross-peak.

2 average of nine possible interpretations per cross-peak.

3 ion can be monitored by the movements of the cross peaks.

4 n-water NOEs from alternate exchange-relayed cross peaks.

5 tifies the sequence positions of 1H-15N HSQC cross-peaks.

6 rom inspection of intraresidual (H1',H6) NOE cross-peaks.

7 d a significant number of intermolecular NOE cross-peaks.

8 221 in PTP1B) to alanine abolished all three cross-peaks.

9 encoded in the signs and intensities of the cross-peaks.

10 y complex perturbs most of the (1)H(N)-(15)N cross-peaks.

11 formational exchange broadening of all F5 NH cross-peaks.

12 sed dramatic perturbation of most of the NMR cross-peaks.

13 ) have less than half the expected number of cross-peaks.

14 unusual intensities observed for DNA-DNA NOE cross-peaks.

15 ngly intense intra- and intermolecular NOESY cross-peaks.

16 well as interior cellulose-surface cellulose cross-peaks.

17 differences in the number of detectable NMR cross-peaks.

18 eled protein show unequivocal attenuation of cross-peaks after membrane binding.

19 A 6-fold increase in cross-peak amplitude was observed in 4-acetylbenzonitril

20 istinguished by examining the time-dependent cross-peak amplitudes for specific excitation and detect

21 relaxation transport in molecules to enhance cross-peak amplitudes.

22 by fitting the mixing time dependence of the cross-peak and diagonal-peak signals to a magnetization

23 d NMR nuclear Overhauser effect spectroscopy cross-peak and hyperfine shift patterns.

24 splitting, amplitude, and line shape of the cross-peaks and diagonal peaks.

25 ed from quantitative simulations of 2QF-COSY cross-peaks and from persistent inconsistencies in exper

26 s, whereas the C-terminal half had fewer NOE cross-peaks and less chemical shift dispersion.

27 While NOE cross-peaks and perturbations of the chemical shifts cle

28 (MQ) spin coherences, which lead to spurious cross-peaks and phase-twisted lines in the 2D (15)N-(13)

29 ined by quantitative simulations of DQF-COSY cross-peaks and used to determine sugar pucker populatio

30 The N-terminal half showed strong NOE cross-peaks and well-dispersed NMR resonances, whereas t

31 f 1H and 15N chemical shifts, sequential NOE cross-peaks, and S2 values of the backbone atoms, and th

32 r the spatial contacts that give rise to the cross peaks are artifacts of sample preparation, we now

33 The cross peaks are observed both at -20 degrees C and at am

34 Cross peaks are selected using local clustering to avoid

35 The resulting cross peaks are used in a simple and direct way to compa

36 The missing cross-peaks are restored upon binding strong agonists su

37 residue 29, together with 85 long-range NOE cross peaks arising from interchain interactions.

38 ed as a function of temperature, showed that cross-peaks arising from the alpha-helical backbone and

39 nterference effects between the diagonal and cross-peaks arising from the sheets, combined with diago

40 o DNA we were able to directly observe three cross-peaks, arising from lysine NH3 groups, with 15N ch

41 complex, were used to classify the exchange cross peaks as follows: (A) a direct NOE with a water pr

42 onventional analysis would have assigned the cross-peaks as being indicative of a chemical exchange p

43 s identified intermolecular pectin-cellulose cross-peaks as well as interior cellulose-surface cellul

44 assignments and extensive analysis of NOESY cross-peak assignments.

45 After binding of ACh, the five cross-peaks associated with cysteines from the C-loop co

46 illatory quantum energy transport but also a cross-peak asymmetry not previously explained by theory.

47 r presence of 50 mm sodium chloride showed a cross peak at 10.25 ppm on the 1H axis and 129 ppm on th

48 tion spectra reveal seven His37 Calpha-Cbeta cross peaks at different pH, some of which are unique to

49 The relative magnitudes of the cross peaks at various population periods measure the pi

50 While Cinn-GEE alone showed a single cross-peak at delta 9.64 ((1)H) and delta 201 ((13)C), t

51 g of selected residues show relatively sharp cross-peaks at chemical shifts that are consistent with

52 PTP1B/Cinn-GEE complex showed three distinct cross-peaks at delta 7.6-7.8 ((1)H) and 130-137 ((13)C).

53 bonded amide(1)H and (15)N spins but also a cross peak between the (1)H chemical shift from bulk wat

54 al station is visible from a newly appearing cross peak between these two moieties.

55 E experiments on 1.3S revealed no detectable cross peaks between biotin and the protein.

56 ncy CN stretching mode contributes to strong cross peaks between CN groups in two different chemical

57 ponding (1)H 2D NOESY NMR spectra display no cross peaks between HPTS and AOT consistent with the HPT

58 In CTAB reverse micelles, clear cross peaks between HPTS and CTAB in the 2D NMR spectra

59 The data showed extensive cross peaks between pectins and cellulose in the primary

60 x of CoA, chloramphenicol and enzyme, 71 NOE cross peaks between protons of coenzyme A and a further

61 mixed, so that only interresidue (13)C-(13)C cross peaks between two differently labeled proteins rep

62 unusual long-range ((5)JH-H) (1)H-(1)H COSY cross-peak between C2-CH3 and C4-H signals.

63 (1)H NMR and ROESY spectra results showed a cross-peak between H-3 proton of beta-cyclodextrin and t

64 We observed a strong intraresidue NOE cross-peak between H1' and H8 of the modified dA(6).

65 ily, the emergence in 2D and 3D spectra of a cross-peak between reactant and product reveals the cis-

66 r characterized by (19)F NMR and show an NOE cross-peak between residues that are located on differen

67 two could be directly determined from NOESY cross-peaks between CH beta protons of the corresponding

68 Nuclear Overhauser enhancement cross-peaks between Co(NH(3))(6)(3+) and the GAAA tetral

69 ed from the intensities of 71 NOE and 33 ROE cross-peaks between coenzyme protons.

70 ween protons of coenzyme A and a further ten cross-peaks between protons of coenzyme A and chloramphe

71 Specifically, we observed strong cross-peaks between the aromatic protons of the Phe ring

72 ase-pairs, no nuclear Overhauser enhancement cross-peaks between the cobalt ligand and neighboring RN

73 NOE cross-peaks between the intercalating phi ligand and pro

74 navalin A, ribonuclease A, and lysozyme show cross-peaks between the IR-active transitions that are c

75 ptide and U46619 using the comparison of the cross-peaks between the NOESY spectra of U46619 with the

76 by intermolecular nuclear Overhauser effect cross-peaks between the peptide and short chain dioctano

77 The cross-peaks between the two arms of the drug are tentati

78 and ion stretches and probing the associated cross-peaks between them, we are afforded a comprehensiv

79 At high GdnHCl concentrations, most cross-peaks broaden and shift, qualitatively indicating

80 multiple spin-spin couplings contributing to cross-peak buildup.

81 most no detectable chemical shift changes of cross-peaks, but some general increase in line width.

82 combination of amide I' and II' diagonal and cross peaks can effectively distinguish between secondar

83 The isotope-dependent amide-I/II cross-peaks clearly reveal the existence of vibrational

84 Cross-peak connectivities observed in the nonexchangeabl

85 Cross-peak connectivity in the nonexchangeable proton sp

86 anomalous" spectrum at pH 7.5, which retains cross-peaks consistent with ionized tyrosinate.

87 could have contributed to line broadening of cross-peaks corresponding to helices H10 and H11.

88 Indeed, the cross-peaks corresponding to the rather hydrophobic beta

89 uted for Cys 187 and 188 shows only two main cross-peaks, corresponding to Cys 123 and 136 from the C

90 were used to confirm the assignment of NOESY cross-peaks critical in defining the global fold of the

91 NOE cross-peaks d(alphaN), d(NN), and d(betaN) provided inte

92 termolecular nuclear Overhauser effect (NOE) cross-peaks define a specific complex in which the Co(NH

93 pectroscopy (HYSCORE) spectra exhibit strong cross-peaks demonstrating strong isotropic coupling of t

94 D, 3D, and 4D MAS spectra, all of which show cross-peak doubling.

95 Cross-peaks due to gas atoms entering or exiting were ob

96 assignments because of the loss of key TOCSY cross-peaks due to the line broadening from a dynamic he

97 p synthase with 1-15N-L-Trp showed a similar cross peak either in the presence or absence of disodium

98 ysis, we obtained signals with well resolved cross-peak features attributed to the couplings within a

99 alpha-helical FP but weak or no (31)P-water cross peak for neutral membranes containing the beta-she

100 H correlation spectra show clear (31)P-water cross peaks for anionic membranes containing the alpha-h

101 Analysis of the buildup rate of the exchange cross peaks for several base-paired imino protons in the

102 hemical shift and the appearance of multiple cross-peaks for certain residues, suggesting that the do

103 shift-pH titration curves are obtained from cross-peaks for reporter protons in BASHD-TOCSY spectra

104 The absence of amide cross-peaks for residues G13 to H19 in the (1)H-(15)N he

105 oops 2 and 3 and the very weak or missing NH cross-peaks for several residues in loops 1 and 3.

106 py of apo-AChBP revealed seven well resolved cross-peaks for the group of cysteines.

107 beta-sheet of ubiquitin through the loss of cross peaks formed between transitions arising from delo

108 19 cross-peaks found between 9.0 and 11.4 ppm are consisten

109 otropic chemical shift data and interhelical cross peaks from magic angle spinning solid-state NMR of

110 The absence of cross-peaks from [14-(13)C]retinal to [4-(13)C]Asp-85 in

111 In H(2)O the 2D-IR shows cross-peaks from large coupling in the alpha-helical con

112 way, to N-betaGRP results in the loss of NMR cross-peaks from the backbone (15)N-(1)H groups of the p

113 ere acquired and overlaid to distinguish the cross-peaks from the main peaks.

114 s is evident from the significant direct NOE cross-peaks from the protons of Co(NH3)6(3+) to GH8, GH1

115 Following the individual cross peaks, however, is complicated and does not establ

116 e narrowed spectra of acyl-proline-NH(2) and cross peaks implying the coupling between its amide-I mo

117 es in 1D (15)N spectra as well as additional cross peaks in 2D (13)C-(13)C and (13)C-(15)N spectra.

118 and strong water-AFP III and water-ubiquitin cross peaks in frozen solution using relaxation filtered

119 The excellent dispersion of cross peaks in the (1)H-(15)N correlation spectrum is co

120 cific assignments of considerably overlapped cross peaks in the 1H-NMR spectrum of a DNA trinucleotid

121 dependent amplitudes and energy positions of cross peaks in the 2.21/1.85 eV (excitation/detection) r

122 Cross peaks in the 2D correlation plots of the spectra r

123 We observe multiple absorption bands but no cross peaks in the 2D IR spectra, which indicates that t

124 are directly measured through the growth of cross peaks in the 2D-IR spectra of CO bound to the heme

125 ns in the line widths and chemical shifts of cross peaks in the HSQC spectrum of CD2d1 during titrati

126 The intensities and line shapes of the cross peaks in the spectrum reflected the kinetic time c

127 the holo-form, Phe17 shows an extra exchange cross-peak in addition to those exchange cross-peaks obs

128 ven histidine residues gave rise to a single cross-peak in the HMQC spectra, and these were assigned

129 with the observation of strong peptide-water cross-peaks in (1)H spin diffusion spectra, these result

130 Here a procedure for the assignment of cross-peaks in (15)N-(1)H correlation spectra of sparsel

131 dsRNA binding induces exchange broadening of cross-peaks in 1H-15N HSQC spectra.

132 orb infrared light and so can be detected by cross-peaks in 2D SFG spectra.

133 se of polarized pulse sequences to elucidate cross-peaks in 2D spectra has been demonstrated in the I

134 e absence of glycerol and produced dispersed cross-peaks in a (1)H-(15)N heteronuclear single quantum

135 easured the intensity buildup of (13)C-(13)C cross-peaks in a series of 2D (13)C correlation spectra

136 from their resolved CalphaH-CepsilonH2 TOCSY cross-peaks in BASHD-TOCSY spectra.

137 The method assists in the identification of cross-peaks in crowded regions of ROESY spectra by movin

138 sults in the selective broadening of certain cross-peaks in the 15N-1H heteronuclear single quantum c

139 he internucleotide A4H8-T3H1' and A4H8-T3H3' cross-peaks in the 1:1 complex of this sequence.

140 roborated by DQF-COSY H2/N1H and ROE N1H/N6H cross-peaks in the 2D NMR spectra of Rh2(DTolF)2{d(ApA)}

141 Intense H8/H8 ROE cross-peaks in the 2D ROESY NMR spectrum of Rh(2)(OAc)(2

142 TolF)2{d(ApA)} is indicated by the H8/H8 NOE cross-peaks in the 2D ROESY NMR spectrum, whereas the fo

143 G)] adduct is evidenced by intense H8/H8 ROE cross-peaks in the 2D ROESY NMR spectrum.

144 The absence of H1'-H2' cross-peaks in the 2QF-COSY spectrum indicates a C3'-end

145 terconvert slowly enough to exhibit separate cross-peaks in the amide region of homonuclear and heter

146 A subset of cross-peaks in the HSQC spectrum of p23 is shifted upon

147 here the weak coupling pairs are revealed as cross-peaks in the indirect dimension separated by the i

148 HYSCORE cross-peaks in the proton region were partially overlapp

149 The cross-peaks in the pure heterodimer 2D IR difference spe

150 ocket, 16 residues had weak or no detectable cross-peaks in the two-dimensional 1H-15N HSQC spectrum

151 The experimental data show no cross-peaks in the twodimensional photocurrent spectra,

152 emonstrate how the structural sensitivity of cross-peaks in two-dimensional correlation plots of chir

153 For AFP III, the water cross peaks indicate that only a portion of its hydratio

154 (2)H distance measurements and (1)H-(1)H NOE cross peaks indicate that the adamantane moiety of the d

155 oton chemical shift and the intensity of the cross-peak indicate a very short hydrogen bond to the ca

156 The disappearing cross-peaks indicate interactions between the beta(2) an

157 lation intensity between the two alpha-helix cross peaks indicated that the more hydrophobic helix fr

158 uronan is extracted retains cellulose-pectin cross peaks, indicating that the cellulose-pectin contac

159 ite, shows two sets of side-chain (15)N-(1)H cross-peaks, indicating conformational flexibility.

160 han for interior cellulose-surface cellulose cross-peaks, indicating that pectins come into direct co

161 ion of the nitrogen-amide proton correlation cross-peaks indicative of a pure, uniformly labeled mole

162 Analysis of cross peak intensities in two-dimensional NMR spectra of

163 uld be estimated from the NOESY diagonal and cross peak intensities.

164 x shows only one set of NMR signals, and the cross-peak intensities are rather uniform, suggesting th

165 oduces complex two-dimensional spectra whose cross-peak intensities are related to correlations in th

166 ensities, and (ii) they significantly reduce cross-peak intensities for strongly J-coupled (13)C site

167 om total relaxation matrix analysis of NOESY cross-peak intensities in restrained molecular dynamics

168 The cross-peak intensities of the loop region in scalar corr

169 The Tr-NOESY cross-peak intensities provided proton-proton distance c

170 Distance restraints were calculated from NOE cross-peak intensities via a complete relaxation matrix

171 A comparison of relative RFDR cross-peak intensities with simulations of the NMR exper

172 lete relaxation matrix analysis of the NOESY cross-peak intensities with the program MARDIGRAS.

173 tion and prohibit the extraction of reliable cross-peak intensities, and (ii) they significantly redu

174 n and subsequent analysis of two-dimensional cross-peak intensities, the chemical identity and mass c

175 uclear Overhauser effect (NOE) spectroscopic cross-peak intensities, yielding accurate distance restr

176 ns were in agreement with nuclear Overhauser cross-peak intensities.

177 e inhibitor, the C.H distance based upon the cross-peak intensity being 2.0 +/- 0.4 A.

178 ar NMR showed a steep decrease in (1)H-(15)N cross-peak intensity for 8 residues in the isolated C-te

179 e conventional use of long mixing time NOESY cross-peak intensity, backbone angles, and hydrogenbondi

180 by significant and selective increased NOESY cross-peak intensity, increase in steric Fe-CN tilt refl

181 Although some NOE cross-peaks involving protons of the hexakis(ethylene gl

182 The NOESY cross-peaks involving U(33) H2' and characteristic of tR

183 ependence of the intensity of these spectral cross-peaks is consistent with an active site tyrosine w

184 to analyze the relaxation data of the type A cross peaks, it was found that the water molecules had s

185 romosome 16A1-B1 (DOXNPH locus) in all three crosses [peak logarithm of odds (lod) score of 92.7, P =

186 on, such that robust monoexponential fits to cross-peaks may be used to determine the diffusion coeff

187 d time scale, and misinterpretation of these cross-peaks may lead to erroneous conclusions should the

188 These cross peaks measure the coupling between the different a

189 tios for the C(epsilon 1)-H and C(delta 2)-H cross-peaks measured with a constant time HSQC experimen

190 rom the C-loop condense into two predominant cross-peaks not observed in the spectrum from the apo pr

191 nge cross-peak in addition to those exchange cross-peaks observed in apo-form.

192 design principles as the HISQC, all the NH3 cross-peaks observed in the HISQC spectrum could be assi

193 Anomalous cross-peaks observed in the NOESY spectra of 2,4-disubst

194 by comparisons of experimental and simulated cross-peaks obtained with high digital resolution.

195 Spectrally resolved cross peaks occur in the off-diagonal region of the 2D I

196 n requires nearly complete assignment of the cross peaks of a refined NOESY peak list.

197 anol (TFE) shows that the individual residue cross peaks of IA(3) become more dispersed in the presen

198 ve attenuation in the 1H-15N HSQC spectra of cross peaks of residues at the end of helix 3 (Ser-58, L

199 However, the amide cross-peak of Leu31 demonstrates a significant urea depe

200 An exchange cross-peak of one phenylalanine in the holoprotein is at

201 TRNOE cross-peaks of bound PC were confirmed to represent intr

202 , which made possible the measurement of NOE cross-peaks of relatively rapidly exchanging amide proto

203 The majority of the (1)H-(15)N HSQC cross-peaks of the folded state show only a limited chem

204 -exchange spectroscopy between corresponding cross-peaks of the magnetically non-equivalent subunits

205 t and intensity decrease of the diagonal and cross-peaks of the sheet's two IR active modes.

206 experiment(s) by superimposing the expected cross-peaks on the mixture spectrum.

207 s, and in each case we see the appearance of cross-peaks on the timescale of a few picoseconds, signa

208 The 2D ES show marked cross-peak oscillations assigned to electronic and vibra

209 Cross-peak oscillations originate from a vibrational wav

210 and presence of glucose, reveal significant cross-peak overlap and heterogeneous peak intensities th

211 c C terminus, is highly flexible, exhibiting cross-peak patterns and transverse relaxation rates that

212 NOE cross-peak patterns in 2D (12)C/(12)C-filtered NOESY spe

213 Analysis of cross-peak patterns in the 2D spectra supports a previou

214 The NOESY cross-peak patterns indicate that the hydrocarbon is int

215 Comparison of chemical shift and NOE cross-peak patterns indicates that the structures of the

216 The 2QF-COSY cross-peak patterns which are resolved have been analyze

217 are unambiguously identified by their unique cross-peak patterns.

218 pancy between the experimental and simulated cross-peak patterns.

219 3 sequential and medium range intrachain NOE cross peaks per chain, characteristic of alpha-helices e

220 In CCl(4), the computed cross-peak portion of the 2D-IR shows evidence of two am

221 f sufficiently high quality that many of the cross peaks, recorded previously from malignant cell mod

222 rotein structure indicates that the observed cross-peak reduction is well correlated with a high perc

223 The experiment exposes the cross peaks, representing the mode coupling, free of the

224 The NOE cross-peaks resulting from the interactions between the

225 Long-range nuclear Overhauser effect (NOE) cross-peaks showed the two helices to be in near proximi

226 In addition, there are many new cross-peaks, showing that the binding is asymmetric.

227 Cross-peak-specific 2D electronic spectroscopy provides

228 we develop 2D electronic spectroscopy using cross-peak-specific pulse polarization conditions in an

229 hibit signs of differential line broadening, cross-peak splitting and chemical shift heterogeneity th

230 N line shapes and higher intensities for NH3 cross-peaks than either HSQC or heteronuclear multiple q

231 re only modestly longer for cellulose-pectin cross-peaks than for interior cellulose-surface cellulos

232 lanine produced perturbations of the His-258 cross-peak that were similar to those detected in the E3

233 Anomalous NOESY cross-peaks that cannot be explained by dipolar cross-re

234 rn delta-proton magnetic anisotropy, and NOE cross-peaks that establish all compounds but 1c and 1g t

235 mal shift and intensity and showed clear NOE cross-peaks to C NHb and NHe.

236 Negative and small NOE cross-peaks to other protons in the sequence reveal a no

237 nce, as demonstrated with the observation of cross-peaks to resonances at the frequency of the water

238 multiplets to singlets in the F1 dimension, cross-peaks to the anomeric protons of the two iduronic

239 eous to the excitation, including pronounced cross-peaks to the bend vibration and a continuum of ind

240 d from the sign and magnitude of NOE and ROE cross-peaks to water identified in 2D NOESY and ROESY ex

241 The D/C cross-peak volume ratio was found to be proportional to

242 The D/C cross-peak volume ratios determined for oleic (18:1), li

243 des a large number of accurate values of NOE cross peak volumes in a decoupled HMQC-NOESY spectrum.

244 significant difference (P < .05) between the cross peak volumes measured for healthy subjects and tho

245 tial Halpha-HN to intraresidue HN-Halpha NOE cross-peak volumes support a predominantly extended conf

246 th crystal structures and the fact that this cross-peak was absent in the heteronuclear correlation s

247 om time-dependent shifts of the stretch-bend cross peak, we determined a lower limit on the lifetime

248 Type A and B cross peaks were analyzed using three models of water dy

249 sidue nuclear Overhauser effect spectroscopy cross peaks were observed at 30 degrees C and a 200 ms m

250 Interestingly, few cross- peaks were observed between the proteins and the

251 proximately 90% of the 949 unambiguous NOESY cross-peaks were assigned automatically with simultaneou

252 Mb data sets collected at various pH values, cross-peaks were assigned to the imidazole rings and the

253 Intraligand NOE cross-peaks were detected for the hydroxyl proton in the

254 These cross-peaks were not observed in the NOESY spectra of Me

255 mellar suspensions of MeDOPE, positive NOESY cross-peaks were observed between the downfield-shifted

256 Strong NOESY cross-peaks were observed between the H5 and H1' of N14

257 Three sets of cross-peaks were observed for each nucleus.

258 Strong cross-peaks were observed from the carboxyl carbon to pr

259 For the (31)P couplings, three sets of cross-peaks were observed in the HYSCORE spectrum, and c

260 The intensities of the triple-helical cross-peaks were stronger for the peptide-amphiphile, co

261 tly (15)N-labeled in P. pastoris, and proton cross-peaks were well dispersed in nuclear Overhauser ef

262 n intensity and disappearance of 1H-15N HSQC cross-peaks, were observed with the addition of either s

263 sensitivity, evidenced by 50-100% additional cross peaks, when compared to alternative processing sch

264 of NP2-ImH mutants exhibit chemical exchange cross peaks, whereas WT NP2-ImH shows no chemical exchan

265 in PTP1B) into alanine had no effect on the cross-peaks, whereas mutation of a conserved active-site

266 agonal allows the accurate identification of cross-peaks which are otherwise obscured by the strong a

267 ugs permitted the assignment of drug-protein cross peaks, which indicate that amantadine and rimantad

268 s separated by one or two bonds (2.5 A) show cross peaks, which therefore identify reference intracha

269 dy revealed the presence of interknuckle NOE cross-peaks, which were interpreted in terms of a more c

270 of free [15N]PGH shows a single 1H-15N HMQC cross peak with delta (1H) = 10.3 ppm and delta (15N) =

271 well as arginines, showed intermolecular NOE cross-peaks with D8PG, providing direct evidence for the

272 The sugar H2' and H2' ' of dC(5) lacked NOE cross-peaks with H8 of dA(6) but showed weak interaction

273 with Phe2, Phe47, and Phe62 showing exchange cross-peaks with minor conformation in (19)F-(19)F nucle

274 In comparison, cross-peaks with similar intensities between the two sta

275 y 15N-labeled protein show two well resolved cross-peaks with weak couplings of approximately 0.3-0.4

276 eral unambiguously identified long-range NOE cross-peaks within the loop region and between TM2 and T