ライフサイエンスコーパス: cross-linking

1 vel of downstream, rather than overall, Taf1 cross-linking.

2 ir wild-type counterparts in response to BCR cross-linking.

3 that FKBP65 regulates LH2-mediated collagen cross-linking.

4 and neovessel branching increase with matrix cross-linking.

5 tuned from extensile to contractile through cross-linking.

6 and form stable helices only after chemical cross-linking.

7 o a second peptidoglycan substrate to attain cross-linking.

8 lated with allogeneic leukocytes or CD3/CD28 cross-linking.

9 at the alignment is effectively preserved by cross-linking.

10 linking to a factor of 20x over conventional cross-linking.

11 ion and anaphylaxis following FcvarepsilonRI cross-linking.

12 and de-adhesion events dependent on receptor cross-linking.

13 isted of control microcapsules (CM), with no cross-linking.

14 ast cells to degranulate in response to IgER cross-linking.

15 influenza in the presence or absence of IgE cross-linking.

16 we identified MUC2 residues involved in this cross-linking.

17 sor was calibrated using a dimer obtained by cross-linking.

18 ed hydrogel matrix was fabricated by protein cross-linking.

19 o identify glycoprotein receptors via ligand cross-linking.

20 d-loaded particles using streptavidin-biotin cross-linking.

21 ficial protease FeBABE and cysteine-cysteine cross-linking.

22 f elastic fibers in vivo, preceding chemical cross-linking.

23 to this biosynthetic pathway to complete the cross-linking.

24 conventional (C-CXL) vs accelerated corneal cross-linking (A-CXL) in pediatric keratoconus (KC).

25 enabled tracking of the effect of the shell cross-linking ability of laccase, or CaCl2, on microcaps

26 impairs lipid II polymerization and peptide cross-linking activities of PBP1b, and that PBP3 relieve

27 this study, which compares the peptidoglycan cross-linking activity of PBP2b from susceptible and res

28 ibitor ammonium to prevent potentially toxic cross-linking activity.

29 Using cross-linking, affinity purification, and mass spectrome

30 is a highly active ADC, which releases a DNA cross-linking agent rather than a microtubule inhibitor.

31 an be defined by incorporating a multivalent cross-linking agent, and this approach has been successf

32 PEEC and tetraethyleneglycol diacrylate as a cross-linking agent, in the form of a flexible thin film

33 teracts with neighbouring DNA molecules as a cross-linking agent.

34 The effect of cross-linking agents on the microcapsules were more sign

35 g hypocellular kidney marrow, sensitivity to cross-linking agents, and decreased size.

36 e use of MVMs has largely been limited to as cross-linking agents.

37 ptibility to DNA alkylating and inter-strand cross-linking agents.

38 In addition, through cross-linking analysis in human cells and biochemical re

39 RNA cross-linking analysis reveals that KOW5 directly contac

40 se residues have a suspected role in protein cross-linking and aggregation, their direct implication

41 To address this, we developed kinetic cross-linking and analysis of cDNAs (chiCRAC), an ultrav

42 Using cross-linking and bioluminescence resonance energy trans

43 es with methacrylates for secondary covalent cross-linking and for reaction with fluorophores for in

44 hiolanes in the micellar cores to induce the cross-linking and gelation of the micellar network.

45 ed these structures by site-directed protein cross-linking and hydroxyl radical footprinting experime

46 UV cross-linking and immunoprecipitation (CLIP), followed b

47 brid and individual-nucleotide resolution UV cross-linking and immunoprecipitation (hiCLIP) is a tran

48 Combining individual-resolution cross-linking and immunoprecipitation (iCLIP) and mass s

49 We used individual cross-linking and immunoprecipitation (iCLIP) to identif

50 allel by individual-nucleotide-resolution UV cross-linking and immunoprecipitation (iCLIP).

51 osteclosion and 24 h PBM through Argonaute 1 cross-linking and immunoprecipitation followed by high-t

52 Cross-linking and immunoprecipitation followed by RNA se

53 mage proteins and DNA through alkylation and cross-linking and interfere with redox homeostasis.

54 yl oxidase-like 2 (LOXL2) catalyses collagen cross-linking and is implicated in the pathogenesis of i

55 We applied Chemical Cross-Linking and Isolation by Pull Down (Chem-CLIP) to

56 ormation of allysine, which enables collagen cross-linking and leads to tissue stiffening and scar fo

57 Chemical cross-linking and MALDI-TOF MS mapped these same regions

58 ron microscopy and verified it with chemical cross-linking and mass spectrometry analysis.

59 ut not for cleavage of Pro-sigma(K) Chemical cross-linking and mass spectrometry of purified, inactiv

60 mobaraensis (MTG) is an important enzyme for cross-linking and modifying proteins.

61 Chemical cross-linking and molecular dynamics are consistent with

62 r with complementary restraints derived from cross-linking and native MS, we built native-like models

63 ic rheometry indicated that transglutaminase cross-linking and niosomes charging of the protein solut

64 n-scaffold linkage: genetic fusion, chemical cross-linking and plug-and-display SpyTag/SpyCatcher con

65 C IFN-alpha responses in the presence of IgE cross-linking and reduced pDC surface FcepsilonRIalpha e

66 Cross-linking and structural studies place the RNaseH do

67 Pluronic F127 micelles, followed by chemical cross-linking and subsequent removal of non-cross-linked

68 al signals provided by stromal cells and BCR cross-linking and was effective against CLL cells harbor

69 released from hydrogel decreased by citrate cross-linking and was higher at neutral pH than pH 2.0.

70 tream of TNF, TLR agonists, antigen receptor cross-linking, and cytokine receptors, all rely on ubiqu

71 Fluorescence, chemical cross-linking, and saturation transfer difference NMR da

72 ology modeling, molecular dynamics, cysteine cross-linking, and voltage clamp experiments, we propose

73 We developed a light-activated cross-linking approach to monitor PKA subunit interactio

74 Using fluorescence quenching and cross-linking approaches, we demonstrate that the Escher

75 lity in the absence of cementing proteins or cross-linking, as occur in other bacteriophages.

76 ed defective in interface 2-specific in vivo cross-linking assays, and formed signaling complexes tha

77 loyed a new proteomic approach, XAP (in vivo cross-linking-assisted affinity purification), coupled w

78 m responsible for channel gating, we perform cross-linking by bifunctional reagents of multiple predi

79 low cross-domain disulfide bond formation or cross-linking by bismaleimides of various lengths.

80 Reversely, ubiquitous TNFRSF receptor(s) cross-linking by CD40 and Fas agonistic antibodies resul

81 ng by myosin motors, as well as cytoskeletal cross-linking by myosins and nonmotor cross-linkers, are

82 by Capture-C do not depend on the degree of cross-linking by performing experiments with varying for

83 ate (BS3), which is used for protein-protein cross-linking can also be applied for cross-linking prot

84 mediator release assay to determine the IgE cross-linking capability of treated and untreated sample

85 s under ion irradiation in reactions such as cross-linking, chain breaking, or reorganization process

86 ed on GO-dLPG via EDC/NHS heterobifunctional cross-linking chemistry leaving the binding sites free f

87 hesis that utilizes readily accessible SiCl4 cross-linking chemistry to create durable hybrid solid-e

88 In this study, we used chemical cross-linking combined with mass spectrometry to capture

89 Cross-linking coupled to mass spectrometry assisted mapp

90 PKA complexes containing RII subunits using cross-linking coupled to mass spectrometry.

91 Cross-linking coupled with mass spectrometry and mutatio

92 itecture of the assembly by a combination of cross-linking coupled with mass spectrometry, X-ray crys

93 Based on cross-linking-coupled mass spectrometry, crystal structu

94 parative cost-effectiveness of early corneal cross-linking (CXL) and conventional management with pen

95 fter standard and accelerated epithelial off cross-linking (CXL) during a long-term follow-up.

96 (CDVA) that underwent ICRS implantation and cross-linking (CXL).

97 In response to DNA cross-linking damage, the Fanconi anemia (FA) core compl

98 enes yield varying cellular sensitivities to cross-linking damage.

99 MS analysis of the large cross-linking data sets requires an automated data analy

100 ssay, which uses time-dependent formaldehyde-cross-linking data to extract kinetic parameters of chro

101 Cross-linking data were compared directly with the cryst

102 l homology and by using constraints based on cross-linking data.

103 Cross-linking decreases MT growth velocities and generat

104 ty of the photoswitch but also to adjust the cross-linking density in the polymer network and to opti

105 y adjusting the light dose, temperature, and cross-linking density.

106 We prepared P-X, a photoactivatable cross-linking derivative of posaconazole.

107 gels that contained weak bonds, and covalent cross-linking diminished viscoplasticity in collagen and

108 x) is a bifunctional electrophile capable of cross-linking DNA.

109 mer is recognized as an important structural cross-linking domain of collagen IV networks that provid

110 Cross-linking domains are also highly disordered in mono

111 Platelet cross-linking during arterial thrombosis involves von Wi

112 riboflavin dosing frequency affects corneal cross-linking efficacy or safety, given that isotonic ri

113 y indicated that mPyTK exhibits higher photo-cross-linking efficiency than a diazirine-based photo-cr

114 n area >100 cm(2) We found that the collagen cross-linking enzyme lysyl oxidase is expressed in all v

115 egulation of lysyl oxidase (LOX), a collagen-cross-linking enzyme, in retinal capillary endothelial c

116 lavin dosing intervals with standard corneal cross-linking (epithelial removal and 30-minute irradiat

117 Photo-cross-linking experiments and biosensor analysis show an

118 c resonance (NMR) spectroscopy combined with cross-linking experiments and competition-binding assays

119 Cross-linking experiments as well as surface Plasmon res

120 Instead, site-specific cross-linking experiments indicate that the suppressor s

121 of site-directed mutagenesis, DA-uptake, and cross-linking experiments that exploited the capacity of

122 Cross-linking experiments with liposome bound Atg18 yiel

123 ng single-molecule fluorescence and in vitro cross-linking experiments with photo-activatable unnatur

124 Cross-linking FcgammaRIIB to stimulatory FcgammaRs throu

125 cluding disaggregation, radial migration and cross-linking fibrin formations.

126 To address this, we have utilized chemical cross-linking followed by mass spectrometry and immunobl

127 rlying mechanism, here we report a method of cross-linking followed by mass spectrometry to define a

128 ers in hippocampal slices using cell-surface cross-linking, followed by Western blot and coimmunoprec

129 artitioned to the polymer surface to prevent cross-linking from occurring.

130 t contains these acetal or ketal moieties as cross-linking functionalities.

131 used a comprehensive integrated approach of cross-linking, hydrogen-deuterium exchange mass spectrom

132 or fluorescent labeling, PEGylation, protein cross-linking, immunoliposome formation, and so on, with

133 Here we used cross-linking immunoprecipitation (CLIP) and ligation of

134 Cross-linking immunoprecipitation revealed that Pum1 and

135 Here, we employed CLIP (cross-linking immunoprecipitation)-seq datasets for 60 h

136 o allow photoliberation (uncaging) and photo-cross-linking in a sequential manner, as well as a click

137 which could promote protein modification and cross-linking in aging human lenses.

138 better define the properties of formaldehyde-cross-linking in budding yeast cells.

139 Predictive cysteine cross-linking in E. coli membranes and PELDOR measuremen

140 This organization is confirmed by cysteine cross-linking in full-length receptors, and it permitted

141 ausal link between Dhb formation and protein cross-linking in live cells.

142 mans coupled with structure-guided disulfide cross-linking in P. aeruginosa suggest that PelC assembl

143 ring segment (ICRS) implantation followed by cross-linking in pediatric keratoconus patients.

144 Dityrosine cross-linking in proteins and peptides are clinical mark

145 LFA-1 cross-linking increased the presence of LAT-GRB2-SKAP1 c

146 The analysis indicates reduced PG cross-linking, increased carboxypeptidase activities, in

147 self-interaction of the domain, and targeted cross-linking indicates that it forms a multimeric array

148 increased affinity mediates adhesion, LFA-1 cross-linking induced the association and activation of

149 IgE cross-linking inhibits critical virus-induced IFN-alpha

150 to dough strength and stability of wheat by cross-linking into gluten aggregates through inter-chain

151 Inducing alpha-helicity through side-chain cross-linking is a strategy that has been pursued to imp

152 Cross-linking is also an attractive strategy for the dev

153 scribed an experimental framework called the cross-linking kinetics (CLK) assay, which uses time-depe

154 ovides insights into antibody-mediated nAChR cross-linking known to cause receptor degradation.

155 interactions with a trace amount of covalent cross-linking leads to hierarchical dual networks with i

156 In addition, via chemical cross-linking, limited proteolysis, and mass spectrometr

157 The effect on cross-linking lysines was quantitatively very similar to

158 of mitochondrial function, we used chemical cross-linking mass spectrometry to identify 2,427 cross-

159 ns, single-particle electron cryomicroscopy, cross-linking mass spectrometry, and structural modeling

160 f Hsp21 based on homology modeling, cryo-EM, cross-linking mass spectrometry, NMR, and small-angle X-

161 rdinates, biochemical analyses and data from cross-linking mass-spectrometry (CLMS), we determine the

162 ber of publications in the field of chemical cross-linking/mass spectrometry (MS) for deriving protei

163 Cross-linking/mass spectrometry is an increasingly popul

164 A cross-linking mechanism has been proposed that involves

165 emplate cores were tested using a reversible cross-linking mechanism, interfacial gelation process or

166 analysis of cDNAs (chiCRAC), an ultraviolet cross-linking method that enabled us to quantitatively m

167 Here, we used a chemical cross-linking method together with molecular dynamics si

168 rinted carbon electrode using glutaraldehyde cross-linking method.

169 ng a facile combined freeze-drying and ionic cross-linking method.

170 ntly, using a disuccinimidyl sulfoxide-based cross-linking MS platform, we mapped the interactions of

171 In the interest of the cross-linking/MS approach, it should be ensured that onl

172 structural information derived from chemical cross-linking/MS experiments.

173 ed this system to investigate the effects of cross-linking MT plus ends and F-actin in Drosophila mel

174 ADCC activity was analyzed using Fc receptor cross-linking, NK cell activation, and influenza-infecte

175 DIBOD (5) permits efficient sequential SPAAC cross-linking of azide-derivatized substrates.

176 temperature promotes the crystallization and cross-linking of calcium (alumino)silicate hydrate (C-(A

177 ed network through the slow autoxidation and cross-linking of catechol moieties.

178 more depolarized preopen holding potentials, cross-linking of F57Bpa with KCNQ1 was slowed, which ind

179 ed an ELISA and multiplex assay to model the cross-linking of Fcgamma receptors (FcgammaR) by Abs, wh

180 A higher degree of cross-linking of glutenins and of inclusion of gliadin i

181 Cross-linking of gp140 Env to M64U1 resulted in earlier

182 ification of the HIV-1 envelope immunogen by cross-linking of gp140 with the CD4-mimetic M64U1 elicit

183 Cross-linking of immunoglobulin E-bound FcepsilonRI trig

184 Cross-linking of mast cell (MC) IgE receptors (Fcepsilon

185 Comparing cross-linking of naturally modified cATPase with the in

186 Cross-linking of nucleic acids to proteins in combinatio

187 Cross-linking of P-X was also blocked by U18666A, a comp

188 In this in vitro system, cross-linking of P-X was inhibited by itraconazole, but

189 The cross-linking of polysaccharides is important for the ce

190 isopeptide bonds and are widely used for the cross-linking of proteins and peptides in food and biote

191 e of mass spectrometry coupled with chemical cross-linking of proteins has become a powerful tool for

192 ompressibility modulus, whereas fixation and cross-linking of proteins with glutaraldehyde increases

193 col consists of the following steps: in vivo cross-linking of RBPs to their bound RNAs; partial RNA d

194 In vitro cross-linking of residues located in the periplasmically

195 was synthesized and applied to nanohydrogel cross-linking of self-assembled reactive ester block cop

196 Here, we use cross-linking of small molecules to isolate chromatin co

197 nce microscopy, and in vivo photoactivatable cross-linking of stalled translocation intermediates, we

198 , the opposite was observed, probably due to cross-linking of starch chains.

199 omparison with the sustained level following cross-linking of the antigen receptor.

200 pes without allergen-specific T cell help by cross-linking of the B cell epitope receptor.

201 Finally, we demonstrate that cross-linking of the BamA barrel does not affect tOmpA f

202 s employed as nanostructured support for the cross-linking of the enzyme laccase with glutaraldehyde

203 e AGPs and the pectic acids give rise to the cross-linking of the exuded adhesive substances, favor s

204 Roadblocks were made either by covalent cross-linking of the HpaII methyltransferase to DNA or t

205 Cross-linking of the P2VP corona of the peripheral block

206 aligned in a magnetic field, immobilized by cross-linking of the surrounding matrix, and exposed on

207 ing evidence suggests that FcgammaR-mediated cross-linking of tumor-bound mAbs may induce signaling i

208 peptides colocalize with, and inhibit fibrin cross-linking, of adherent platelets.

209 To determine whether the effects of cross-linking on gating observed are the result of switc

210 in regions of the cell wall with reduced PG cross-linking on PG units that have stems terminating in

211 The effect of glycation and cross-linking on protein breakdown and release of beta-c

212 quantify the influence of dreierketten chain cross-linking on the anisotropic mechanical behavior of

213 t contain disulfides, the profound impact of cross-linking on the behavior of the protein polymer, th

214 did not affect their calcium flux after BCR cross-linking or the proliferation of otherwise-unmodifi

215 rest, upon an adaptive-type activation (IgE cross-linking), or upon an innate-type activation (IL-33

216 consistency coefficient (K); however, starch cross-linking over time by the generated trisodium citra

217 Their cross-linking partners include His231, His292, His440, a

218 Docking models and cross-linking patterns between the Fe protein and flavod

219 n proteolytic cleavage patterns and chemical cross-linking patterns were consistent with known nucleo

220 n photoisomerization, photo-cross-linking/un-cross-linking, photoreduction, and so forth.

221 s-links in NMDA receptors and found that the cross-linking produced stationary gating kinetics that d

222 The actin cross-linking protein Filamin has an important role in t

223 PARP-1 along with collagen biosynthesis and cross-linking protein in HG condition.

224 rated that alpha actinin 4 (ACTN4), an actin-cross-linking protein known to coordinate cytoskeletal o

225 the evolutionarily conserved actin bundling/cross-linking protein plastin is instrumental for the ge

226 x 10(-10)), encoding plectin, a cytoskeletal cross-linking protein that contributes to integrity of c

227 Filamin A (FlnA) is an important actin cross-linking protein that is required for cellular proc

228 ed collagen biosynthesis as well as collagen cross-linking protein, P4HA1 and PLOD2 were observed alo

229 dict paralog-specific behaviors of different cross-linking proteins and identify a zone of optimal ac

230 rotein cross-linking can also be applied for cross-linking proteins and phosphatidylethanolamine (PE)

231 lay between the density of myosin motors and cross-linking proteins and the rigidity, initial orienta

232 works of actin filaments, myosin motors, and cross-linking proteins at biologically relevant time and

233 The role of actin cross-linking proteins in cortical dynamics is still inc

234 roxyproline, which is essential for collagen cross-linking purposes in the human body.

235 Notably, we observed that formaldehyde-cross-linking rates can vary dramatically for different

236 s that catalyzes a single intramolecular C-C cross-linking reaction in the biosynthesis of mycocyclos

237 For other interactions, we found the cross-linking reaction occurred on the same time scale o

238 polymer electrolyte was synthesized by photo cross-linking reaction using PEEC and tetraethyleneglyco

239 ric network is formed via a laccase-mediated cross-linking reaction.

240 zed cryogel monoliths through a photoinduced cross-linking reaction.

241 ne units in combination with dehydrogenation cross-linking reactions within the polymer chains leads

242 ound to give robust and site-selective photo-cross-linking reactivity in E. coli when placed at an ap

243 d data suggest that ZapD acts as a molecular cross-linking reagent between FtsZ protofilaments to enh

244 Here we report a novel role for the actin cross-linking regulator filamin A (FLNa) in Vpu anti-tet

245 Importantly, collagen cross-linking requires fibronectin (FN).

246 ranslational modification by positioning the cross-linking residues of the precursor peptide within t

247 Drosophila were determined by Hi-C (chemical cross-linking, restriction digestion, ligation, and high

248 strongly hindered protein breakdown, whereas cross-linking resulting from beta-elimination reactions

249 Furthermore, BMBs activated via IgE cross-linking selectively induced immune response genes

250 Site-specific cross-linking shows that the chromodomains and ATPase mo

251 h the peptides containing the known collagen cross-linking sites and the MMP-1 cleavage site in colla

252 under-hydroxylation effect at helical domain cross-linking sites in skin, bone, tendon, aorta, and co

253 r-hydroxylation seen in individual chains at cross-linking sites.

254 tophoresis (T-ionto CL) and standard corneal cross-linking (standard CL) for the treatment of progres

255 e acylation of the enzymes that catalyze the cross-linking steps in the biosynthesis of their peptido

256 and IL-3, without exogenous allergen or IgE cross-linking stimuli.

257 Co-immunoprecipitation and chemical cross-linking studies previously revealed that the N-ter

258 Cross-linking studies revealed that an equal input of hE

259 Together with cross-linking studies, the solution structural data lead

260 UV-induced cross-linking suggested that F56Bpa interacts with KCNQ1

261 with similar affinities before and after the cross-linking, suggesting that the binding site is prese

262 o FcgammaRs on immune cells induces FcgammaR cross-linking that leads to cellular effector functions,

263 parently resulting from significant covalent cross-linking that occurs during the treatment of their

264 Cross-linking the dialyzed emulsion with transglutaminas

265 LOX) remodels the tumour microenvironment by cross-linking the extracellular matrix.

266 s but retain their physiological activity of cross-linking the peptidoglycan, the major constituent o

267 rboxylate) promotes rapid network formation, cross-linking the reactive polymer chains.

268 After UV-induced thiol-ene cross-linking, the core of the PFC emulsion remains in l

269 beta-sheet peptides to provide non-covalent cross-linking through beta-sheet assembly, reinforced wi

270 Ca(2+) or activate MAPK signaling after BCR cross-linking, thus displaying a signature of B cell ane

271 This further increases the density of photo-cross-linking to a factor of 20x over conventional cross

272 aced at the dimer interface showed increased cross-linking to bound preproteins.

273 me-resolved limited proteolysis and chemical cross-linking to examine nucleotide-induced structural c

274 ere, we have used unnatural amino acid photo-cross-linking to investigate the dynamic interactions th

275 nition motif 2 (RRM2) showed reduced in vivo cross-linking to RNA and reduced chromatin occupancy.

276 We utilize cysteine cross-linking to verify positions and orientations of re

277 d DDSs, relying on photoisomerization, photo-cross-linking/un-cross-linking, photoreduction, and so f

278 Formaldehyde-cross-linking underpins many of the most commonly used e

279 In bone marrow-derived mast cells, IgE cross-linking upregulated 785 genes, including Egr2, Ccl

280 ules and post self-assembly undergo covalent cross-linking using copper catalyzed click chemistry.

281 clinical outcomes of transepithelial corneal cross-linking using iontophoresis (T-ionto CL) and stand

282 Cross-linking was inhibited by itraconazole but not by k

283 P-X cross-linking was not prevented by deletion of the N-ter

284 Cross-linking was performed 1 month subsequently.

285 In contrast, P-X cross-linking was reduced when NPC1 contained a point mu

286 nd unnatural amino acid-mediated protein-DNA cross-linking, we have determined, for a library of 4(10

287 Using site-specific cross-linking, we show that internal poly(dA:dT) tracts

288 Using RNA immunoprecipitation and UV cross-linking, we show that WT1 binds preferentially to

289 ity of the N terminus and loop2 via cysteine cross-linking, which actually caused significant inhibit

290 ermore, we showed that the mechanism of this cross-linking, which involves both singlet oxygen and ra

291 CD4 binding site (CD4bs) of gp140 by stable cross-linking with a 3-kDa CD4 miniprotein mimetic, serv

292 urred on muropeptides fragments with reduced cross-linking with a pentapeptide stem that terminated i

293 Attempted cross-linking with a serine-containing protein did not p

294 hus, squaramate-RNAs provide specificity for cross-linking with defined groups in complex biomolecule

295 ody and protein G was stabilized by chemical cross-linking with glutaraldehyde and the capture abilit

296 (PEI) molecules for efficient interparticle cross-linking with glutaraldehyde.

297 his study, we report that the integrin LFA-1 cross-linking with its ligand ICAM-1 in human PBMCs or C

298 mical proteomics approach relying upon photo-cross-linking with synthetic diazirine-containing RNA pr

299 This study also shows that isotope-encoded cross-linking with the "mass tags" selection criteria al

300 Quantitative comparison of cross-linking within a complex of RIIbeta and Cbeta, wit