ライフサイエンスコーパス: crude extract

1 ivity about 60 times higher than that of the crude extract.

2 , purified TBP, or with protein from a yeast crude extract.

3 estion decreased 51-78% when compared to the crude extract.

4 placed from its binding site by H4TF1 in the crude extract.

5 sence of yet unidentified factors present in crude extract.

6 orm, and n-butanol fractions, as well as the crude extract.

7 ciscana, across a range of concentrations of crude extract.

8 binding protein), and an adenovirus-infected crude extract.

9 ndem affinity purified RNA polymerase I from crude extract.

10 ut none of them had higher activity than the crude extracts.

11 onal effect on transcriptional initiation in crude extracts.

12 lase activity for both purified proteins and crude extracts.

13 d to specifically bind biotinated Cin8p from crude extracts.

14 cificity as that seen intracellularly and in crude extracts.

15 erized after stabilization to proteolysis in crude extracts.

16 eacted with a 65-kDa protein in M. bovis BCG crude extracts.

17 wild type, and is catalytically inactive in crude extracts.

18 A1 led to an increase in the Vmax of CPA2 in crude extracts.

19 d a loss of Pab1p-stimulated PAN activity in crude extracts.

20 indicated the same molecular mass as that in crude extracts.

21 ed rapid DNA extraction methods that produce crude extracts.

22 or detecting these low-abundance proteins in crude extracts.

23 fically retrieved PflB from Escherichia coli crude extracts.

24 ed with three different solvents to yield 72 crude extracts.

25 nsive spectral information of metabolites in crude extracts.

26 fenugreek and 13 from bitter melon in active crude extracts.

27 dney bean) lectins, were coprecipitated from crude extracts, 0.05 to 0.4% crude protein, in a single

28 CdtA was present in two forms in crude extracts, 25 and 18 kDa; only the 18-kDa fragment

29 The ACs content reduced to 86.4% (crude extract), 60.9% (ACs-EEX), 36.0% (ACs-EES), 64.8%

30 s with the different components of a natural crude extract after being separated by a coupled HPLC co

31 es the feasibility of bioprofiling a natural crude extract after being separated in HPLC using microf

32 ative estimation of triterpenic acids in the crude extracts an NMR based methodology was used and com

33 ificant seasonal and solvent effects for the crude extract and all the fractions except for the polar

34 s, antioxidant activities, and PPC among the crude extract and fractions, albeit to different extends

35 after in vitro digestion of a wheat gliadins crude extract and further characterized by LC-ESI-MS/MS.

36 The crude extract and its fractions were determined by measu

37 s; and (3) co-incubation of LPS with a pecan crude extract and its fractions.

38 haride (LPS); (2) pre-treatment with a pecan crude extract and its fractions; and (3) co-incubation o

39 Yields were measured for the crude extract and its neutral, organic, basic, polar and

40 In crude extract and partially purified preparations from E

41 In DNase I footprinting with both crude extract and purified protein, Mor protected Pm seq

42 ity of partially purified AtPAP15 from plant crude extract and recombinant AtPAP15 expressed in bacte

43 The Kd values of SA binding to crude extract and to recombinant BiP were 45.2 and 54.6

44 This enzyme was purified 90-fold from crude extracts and characterized.

45 specific binding properties as observed with crude extracts and correlated with the elution of a 36-k

46 hondria, and showed comparable activities in crude extracts and in mitochondria isolated from transfe

47 cond, Bur1 and Bur2 coimmunoprecipitate from crude extracts and interact in the two-hybrid system; an

48 ith the purified enzyme and also tested with crude extracts and membrane fractions from bacteria and

49 Analysis of the reaction with crude extracts and purified components demonstrated that

50 ication, we fractionated adenovirus-infected crude extracts and tested them in an in vitro replicatio

51 iously described assays, one employing moeA- crude extracts and the other utilizing a defined system.

52 r DH had positive proliferative responses to crude extracts and two purified proteins, protein IV (83

53 ays were performed on whole tissue segments, crude extracts, and purified extracts.

54 SMG-1 and SMG-2 coimmunoprecipitate from crude extracts, and this interaction is maintained in sm

55 The antioxidant capacities of the crude extract, aqueous and ethyl acetate partitions of L

56 rk cartilage may work as a cancer retardant, crude extracts are ineffective.

57 or serum testing for allergen-specific IgE; crude extracts are the basis for most evaluations.

58 2 gene abolished an STRE-binding activity in crude extracts as judged by both gel mobility-shift and

59 detected in the stem (1105.14+/-243.10 mug/g crude extract), as SF was lower than the detection limit

60 on plants exposed to male fly emissions (or crude extracts), as well as enhanced induction of the ke

61 gels produced, after 2h, by chymosin and the crude extract at pH 3.

62 on of miraculin using IMAC was achieved with crude extract at pH 7, Tris-HCl binding buffer at pH 7 a

63 Crude extract based cell-free protein synthesis (CFPS) h

64 s that had been completely suppressed in the crude extract became readily detectable and quantifiable

65 NG protein inhibited base excision repair in crude extract by at least 90%.

66 apidly fractionate a multigram quantity of a crude extract by centrifugal partition extraction (CPE).

67 of CPC involves a multistep treatment of the crude extract by precipitation with ammonium sulphate, f

68 approximately 90%, only suppressed repair in crude extracts by a maximum of approximately 70%.

69 ta, ddCTP, decreased base excision repair in crude extracts by approximately 50%, whereas the Polalph

70 particles formed in E. coli were examined in crude extracts by electron microscopy.

71 d information on the chemical composition of crude extracts can be generated.

72 ivity and less odour, compared with those of crude extract (CE).

73 ied from Escherichia coli was incubated with crude extracts (CE) from strains RN6390 (rsbU) and SH100

74 me as well as the aminopeptidases present in crude extract cleaved preferentially Phe-pNA.

75 ductase were two to threefold lower in MP101 crude extracts compared with the BW25113 wild-type strai

76 ed GDI as well as Escherichia coli (E. coli) crude extract containing GDI, suggest that this analogue

77 Supershift analysis of EMSAs using crude extracts containing mycMga2p indicated that Mga2p

78 Crude extracts containing PceA--harboring either a nativ

79 P extract by i.p.injection indicate that the crude extract daidzin has approximately 10 times greater

80 es REV7 and, using immunoprecipitations from crude extracts, demonstrate that, in addition to the pol

81 stern blots with the recombinant proteins in crude extracts demonstrated that the monoclonal antibodi

82 the binding of RPA to single-stranded DNA in crude extracts derived from both C.B-17 and SCID cells.

83 le from that of the enzyme in a freshly made crude extract, even after storage of the pure sample for

84 Crude extracts exhibited a high level of antimicrobial a

85 The fenugreek ethyl acetate crude extract (FGE3) demonstrated the highest antioxidan

86 Biochemical assay of crude extracts for 6-phosphogluconolactonase enzymatic a

87 Furthermore, enzyme in freshly-prepared crude extracts forms only very small amounts of GS-TriCH

88 ion in foods, confirmed that the brown algae crude extracts, fractions and pure components are compar

89 ird-stage larvae (L3), molting L3 (mL3), and crude extract from adult males (M-OvAg) were compared to

90 e residues, reaching 68.5 lipase U/g for the crude extract from fractions called frit.

91 tro with two different luminal proteins in a crude extract from sheep pancreas microsomes.

92 gents from natural products, we identified a crude extract from Tacca chantrieri that initiated Taxol

93 -chloro-l-tryptophan after reactivation with crude extract from the host strain, suggesting that an a

94 es of vhs protein were used in these assays: crude extract from virions or protein translated in a re

95 otein synthesis (CFPS) platform that employs crude extracts from a genomically recoded strain of Esch

96 A RDRP activity was identified in crude extracts from C. parvum sporozoites and products o

97 the addition of molybdenum in an assay using crude extracts from E. coli moeA(-) cells.

98 Crude extracts from Escherichia coli expressing AtzD hyd

99 medical practitioners began to believe that crude extracts from glands or other organs, when prescri

100 in sensitivity allows 230 peaks detected in crude extracts from only a few pooled neuronal tissues a

101 isotope dilution) of carotenoids present in crude extracts from plant tissues and whole cells; (iii)

102 ein reacted with proteins of similar size in crude extracts from Pseudomonas putida and Pseudomonas f

103 etabolism is established using LC-MS data of crude extracts from shaking flask fermentations.

104 mRNA translation in crude extracts from the yeast Saccharomyces cerevisiae i

105 greater than 90% of the activity detected in crude extracts from wild-type yeast cells.

106 While the particle morphology visualized in crude extracts generally was the same as that visualized

107 Since phosphatidylcholine species in crude extracts have been shown to cause ion suppression

108 riments using both purified RAG proteins and crude extracts have failed to detect trans cleavage of p

109 Fractionation of these crude extracts identified replication factor C (RFC), pr

110 ation step is always performed in vivo or in crude extracts in the face of competition from natural a

111 that the Fe-S cluster synthesis observed in crude extracts in vitro may involve some of the componen

112 tify the possible bioactivity in the several crude extracts is highlighted.

113 ins, and antioxidant activities, followed by crude extract, LWM-FP, and LMW, respectively.

114 f both MgATP and wild-type Fe protein to the crude extracts made by A. vinelandii UW97.

115 Compared with crude extract, normal hexane fractions (NHFs) have a rem

116 In this work crude extracts obtained from the edible part of Chamelea

117 uring in vitro physiological metabolism in a crude extract of bacteriophage T7-infected cells.

118 activated in vitro by factors present in the crude extract of E. coli and to a much smaller extent in

119 Four enzymatic activities in the crude extract of E. coli were found that can provide sul

120 and OAG) were tentatively identified in the crude extract of grapefruit seeds by ESI mass spectromet

121 ydrophobic interaction chromatography of the crude extract of mucoid P. aeruginosa 8821, a CF isolate

122 ocess for the purification of miraculin from crude extract of S. dulcificum.

123 Using a crude extract of Streptomyces collinus, we have resolved

124 (1), trinactin (2), and tetranactin (3) in a crude extract of Streptomyces sp. AMC 23 in the precurso

125 55 and 585 helped to identify nigericin in a crude extract of Streptomyces sp. Eucal-26 by means of p

126 This insertion reaction required crude extract of the DeltanifHDK A. vinelandii strain CA

127 as the microtubule-active constituent in the crude extract of the Mountain torchwood, Amyris madrensi

128 ference standards, and (iii) LC-QTOF data of crude extracts of 10 strains of laboratory grown culture

129 Crude extracts of a PHO13-overexpressing strain showed a

130 tion factor was detected on the PC arrays in crude extracts of all stages of the seedling cotyledons,

131 Crude extracts of Ataulfo exhibited polyphenol oxidase (

132 f hMS holoenzyme also were examined by using crude extracts of baculovirus-infected insect cells cont

133 erall, the findings provide information that crude extracts of brown edible seaweeds, phenolic compou

134 uctase activity (2 to 39 nmol min-1 mg-1) in crude extracts of C. thermoaceticum.

135 stingly, the carbonyl content of proteins in crude extracts of cells harvested after 48 h of stationa

136 al RNA processing in immunoprecipitates from crude extracts of cells.

137 e, RNase MRP RNA, in immunoprecipitates from crude extracts of cells.

138 Crude extracts of cultured parasites, prepared simply by

139 Crude extracts of defatted seeds were analysed by means

140 In contrast, deacetylase activity in crude extracts of E. coli was insensitive to EDTA, and t

141 of sensitivity employed Eu3 was detected in crude extracts of embryos but not non-embryonic tissues

142 When crude extracts of French-pressed or osmotically shocked

143 Crude extracts of Geobacter sulfurreducens catalyzed the

144 teins, pyronin-modified Rnase A was added to crude extracts of human HeLa cells.

145 or have only a subarthritogenic effect, and crude extracts of human osteoarthritic cartilage induced

146 pullulanase or D-enzyme could be detected in crude extracts of leaves of the mutant.

147 bstrates were also efficiently methylated in crude extracts of loblolly pine secondary xylem.

148 Km(app) values obtained in crude extracts of male or female rat liver and post-benz

149 rified Sco1p sediments identical to Sco1p in crude extracts of mitochondria from wild type yeast or f

150 The CO oxidation activity in crude extracts of nitrate (30 mM)-supplemented cultures

151 n of excess heavy metal, as was the case for crude extracts of P. aeruginosa.

152 A screening of marine sponges revealed that crude extracts of Psammocinia sp. exhibited potent 15-hL

153 the cbb(I) promoter region were detected in crude extracts of R. sphaeroides.

154 ) but also the major ecdysteroids present in crude extracts of Silene otites, Silene nutans, and Sile

155 The enzyme was purified from crude extracts of soybean root nodules approximately 100

156 ic activity could be detected in vitro using crude extracts of stationary phase cultures, but was abs

157 In the present report, we show that crude extracts of Streptococcus agalactiae catalyze the

158 lel to these experiments, in vitro assays on crude extracts of T. pseudonana demonstrated mean inhibi

159 Comparison of the protein profile of crude extracts of the B. fragilis strains revealed that

160 As isolated and as present in crude extracts of the files, this xanthine dehydrogenase

161 In addition, crude extracts of the mutant failed to convert 4-hydroxy

162 In addition, Sll1951 was prominent in crude extracts of the wild type, indicating that it is a

163 Crude extracts of this strain showed no aspartyl peptida

164 Incubation of CGA with crude extracts of tomato fruits led to the formation of

165 Immunoblotting analysis of crude extracts of transfected fibres demonstrated the sy

166 ever, hydroxylase activity was detectable in crude extracts of vegetative tissues.

167 When crude extracts of wt, HypA:kan and HypB:kan were separat

168 Analyses of crude extracts of yeast expressing L183P-hGALE demonstra

169 , we propose (i) a one-step fractionation of crude extracts on P11 phosphocellulose, followed by (ii)

170 Previously, intensive in vitro studies with crude extract or purified enzyme concluded that the atta

171 Increased levels of molecular chaperones in crude extracts, particularly DnaJ, indicated a rather in

172 n, where the influence of binding buffer pH, crude extract pH and imidazole concentration in elution

173 izable and easily accessible high-throughput crude extract preparation method for CFPS based on sonic

174 geting vankyrin detected a 19-kDa protein in crude extracts prepared from the 3 days p.p. fat body.

175 Electrophoretic mobility shift assays using crude extracts prepared from wild-type and argP-defectiv

176 Substances, or crude extracts, produced by worms and responsible for th

177 ng purification it was observed that NifW in crude extracts ran above the predicted molecular weight

178 The soluble pool of FP21 from crude extracts resolves chromatographically into two fra

179 .05+/-246.18 and 111.94+/-16.49 mug/g in the crude extract, respectively), while only SE was detected

180 Plant crude extracts showed 6-7 fold higher enzyme activity th

181 It was found that 30 out of 106 crude extracts showed more than 80% inhibition of the al

182 Preliminary screening in Escherichia coli crude extracts showed that their presence during protein

183 , exhibited 12% of the wild-type activity in crude extracts, suggesting that Mn remains bound; howeve

184 tro was primed by DnaG primase, whereas in a crude extract system that had not been fractionated, it

185 The optimal conditions were: a ratio of crude extract/t-butanol of 0.87 (v/v), saturation in amm

186 a Doehlert design with 3 variables (ratio of crude extract/t-butanol, the ammonium sulphate saturatio

187 e have detected several proteins in S. pombe crude extracts that bind to the oligonucleotide and ars3

188 er to evaluate the assay for glycoprotein in crude extract, the glycoprotein was separated by SDS-PAG

189 In crude extracts, the hydroxylaminobenzene mutase was stab

190 he precursor ions of all other lipids in the crude extracts, thereby enabling their unambiguous assig

191 he ability of both purified RAG proteins and crude extracts to cleave DNA substrates in trans is a fu

192 d adsorption of the biotinylated module from crude extracts to immobilized streptavidin.

193 of the native PKA has also been detected in crude extracts using kemptide as a substrate.

194 emical profile, was produced from grape seed crude extract ( Vitis vinifera; enriched grape seed extr

195 onger isotope fractionation was observed for crude extracts vs intact cells of Sulfurospirillum multi

196 with a methanol/chloroform solution, and the crude extract was directly analyzed by DESI-MS, with a t

197 The crude extract was first separated by low pressure liquid

198 Crude extract was obtained from intestines of fish Nile

199 Moreover, a skin prick test using the crude extract was positive for A. simplex but negative f

200 A phenolic crude extract was prepared from pecans and separated by

201 The crude extract was prepared with acetone (60% v/v) and pu

202 on with homodimeric Fe proteins contained in crude extracts was accomplished by construction of a sev

203 Crude extracts were used for characterization of enzyme

204 This method enables clean-up of crude extracts within 18min and screening and confirmati