ライフサイエンスコーパス: cryoEM

1 cryoEM images of WEEV were used to determine the first t

2 A cryoEM reconstruction of CAV21 complexed with ICAM-1 sho

3 s-linked heads and tails and produced an 8-A cryoEM reconstruction of the cross-linked head-tail comp

4 We have generated an 8-A cryoEM reconstruction of this state for myosin V and use

5 ing actin's D-loop region based on our 3.9 A cryoEM reconstruction suggests that oxidation by Mical r

6 Guided by a cryoEM-based model of the hexagonal lattice of HIV-1 CA,

7 Here, we present a cryoEM reconstruction of a yeast preinitiation complex a

8 Here, we present a cryoEM study of a tubular assembly of CA and a high-reso

9 esolution is not always uniform throughout a cryoEM map, and it can be useful to estimate the resolut

10 al. show us how they are put together with a cryoEM structure of the 90S processome that initiates ri

11 ical importance of the N-terminal 10 aa, and cryoEM reconstruction of the one with six residues trunc

12 itor the process with biochemical assays and cryoEM structural analysis in parallel.

13 wild-type B19 with the crystal structure and cryoEM reconstruction of recombinant B19 particles consi

14 odeling Y30, F32 and I34 of C11 in available cryoEM pol III structures predicts a hydrophobic patch t

15 ccess in ribosome structure determination by cryoEM has opened the door to defining structural differ

16 he structure of E.coli RecBCD, determined by cryoEM at 3.8 A resolution, with a DNA substrate that re

17 (LDL.LDLr) at extracellular pH determined by cryoEM.

18 Here, by cryoEM of KSHV at 6-A resolution, we show that SCP forms

19 secondary structural elements identified by cryoEM locates 15 amphipathic alpha-helical regions on t

20 Direct observation by cryoEM reveals that this occupancy-enhancing N-terminal

21 with dimensions similar to those observed by cryoEM; on the other hand, the hydrophobic effect shrink

22 Images obtained by cryoEM showed that the extracellular stick-like domain o

23 lence factor of Streptococcus pneumoniae, by cryoEM.

24 rticle is determined to 10.4 A resolution by cryoEM image reconstruction.

25 of rice dwarf virus that has been studied by cryoEM at 6.8A.

26 ese mechanistic principles were validated by cryoEM analysis of an expanded variant of Hsp16.5 in com

27 V MCP capsomers were subtracted from the CIV cryoEM reconstruction, showed that there are at least th

28 This combined cryoEM-Nanogold labeling study has provided the first lo

29 Here, by direct electron-counting cryoEM, we have determined the structures of the Leishma

30 ticle determined by electron cryomicroscopy (cryoEM) and single-particle analysis at about 4.3 A reso

31 ing mechanism using electron cryomicroscopy (cryoEM) and small-angle X-ray scattering.

32 , was determined by electron cryomicroscopy (cryoEM) and three-dimensional reconstruction at 23-A res

33 xes.Single-particle electron cryomicroscopy (cryoEM) can circumvent some of the problems of x-ray cry

34 ementing this work, electron cryomicroscopy (cryoEM) has provided relatively low-resolution structure

35 econstructions from electron cryomicroscopy (cryoEM) of bovine papillomavirus at 9 A resolution with

36 w-resolution (20 A) electron cryomicroscopy (cryoEM) structures of this gp140 trimer, which adopts tw

37 have determined by electron cryomicroscopy (cryoEM), at about 11 A resolution, the structure of a cl

38 ne papillomavirus by electron cryomicrosopy (cryoEM), at approximately 3.6 A resolution.

39 majority of the vertex model in well-defined cryoEM density.

40 s not induce oligomer heterogeneity enabling cryoEM analysis of the complexes.

41 tein assemblies at 20 A, and an experimental cryoEM map at 23.5 A resolution.

42 otide-bound and -free states, and the fitted cryoEM structure of the D2 hexamer ring, which provide a

43 site for structure-based drug design and for cryoEM to become widely interesting to pharmaceutical in

44 econstructions of comparable resolution from cryoEM images of asymmetric particles.

45 n its small 2.5 nm size and detectability in cryoEM.

46 sids conjugated to Au102_C6MI were imaged in cryoEM for single particle reconstruction to localize Au

47 identifying regions or domains or motifs in cryoEM maps of large macromolecular assemblies (such as

48 ld, and the bound Nanogold was visualized in cryoEM images of the reduced, gold-labeled receptor.

49 epresentations of local regions in the input cryoEM maps.

50 d to be intrinsically disordered, and little cryoEM density is observed for them.

51 Electron cryo-microscopy (cryoEM) and image analysis showed that, compared with th

52 Electron cryo-microscopy (cryoEM) and X-ray solution scattering were used to show

53 combined modes of electron cryo-microscopy (cryoEM), we have solved the structure of the Pyrococcus

54 Cryoelectron microscopy (cryoEM) and single-particle image reconstruction methods

55 olution by means of cryoelectron microscopy (cryoEM) and three-dimensional image reconstruction.

56 t-of-its-kind BSL-3 cryoelectron microscopy (cryoEM) containment facility.

57 eling methods using cryoelectron microscopy (cryoEM) density maps as constraints are promising approa

58 The cryoelectron microscopy (cryoEM) image reconstruction of CAV21 is consistent with

59 been determined by cryoelectron microscopy (cryoEM) image reconstruction to a resolution of approxim

60 esolution of 6 A by cryoelectron microscopy (cryoEM) single-particle image reconstruction.

61 were determined by cryoelectron microscopy (cryoEM) to 7.5-A and 11.3-A resolution, respectively, as

62 this paper, we used cryoelectron microscopy (cryoEM) to visualize destabilized mutants of T4 lysozyme

63 onance (SSNMR), and cryoelectron microscopy (cryoEM), have enabled high-resolution insights into thei

64 electrophoresis and cryoelectron microscopy (cryoEM), the ability of the reconstituted LDL receptor t

65 s, and imaged using cryoelectron microscopy (cryoEM).

66 Additionally, cryo-electron microscopy (cryoEM) analysis of third-side insertion mutants showed

67 electron-counting cryo-electron microscopy (cryoEM) and asymmetric reconstruction.

68 ed single particle cryo-electron microscopy (cryoEM) and led to a wave of near-atomic resolution (typ

69 Using cryo-electron microscopy (cryoEM) and single particle cryo-electron tomography (SP

70 g and fitting into cryo-electron microscopy (cryoEM) density maps.

71 Cryo electron microscopy (cryoEM) has emerged as an excellent tool for resolving h

72 itectures by using cryo-electron microscopy (cryoEM) image reconstruction techniques.

73 was determined by cryo-electron microscopy (cryoEM) image reconstruction.

74 bS antagonist; its cryo-electron microscopy (cryoEM) image suggests that the N-terminal domains of th

75 is (PAGE) and cryogenic electron microscopy (cryoEM) imaging.

76 5.3 A resolution, cryo-electron microscopy (cryoEM) map of Chikungunya virus-like particles (VLPs) h

77 se components into cryo electron microscopy (cryoEM) maps of their assemblies.

78 were determined by cryo-electron microscopy (cryoEM) reconstruction to resolutions varying from 8.5 t

79 11 single-particle cryo-electron microscopy (cryoEM) reconstructions of the complex of bacterial 30S

80 nometer resolution cryo-electron microscopy (cryoEM) structural analysis of an adenoviral vector, Ad3

81 ongatus KaiB and a cryo-electron microscopy (cryoEM) structure of a KaiBC complex.

82 three-dimensional cryo-electron microscopy (cryoEM) structure of an infectious ZIKV (strain H/PF/201

83 ella, based on the cryo electron microscopy (cryoEM) structure of the Methanospirillum hungatei archa

84 nometer resolution cryo-electron microscopy (cryoEM) structures of HD5 complexed with both neutraliza

85 e, consistent with cryo-electron microscopy (cryoEM) tomography, within which the boundaries of signa

86 Single-particle cryo electron microscopy (cryoEM) typically produces density maps of macromolecula

87 this T6SS and, by cryo electron microscopy (cryoEM), show the structure of its post-contraction shea

88 lies based on cryogenic electron microscopy (cryoEM), the dimerization interface is substantially dis

89 Here, based on cryo-electron microscopy (cryoEM), we report a 7-A resolution structure of the inf

90 As an example, we apply these methods to new cryoEM maps of the mature bacteriophage P22, reconstruct

91 organizations corresponding to the observed cryoEM density map.

92 cines.The result shows that a combination of cryoEM and molecular modeling can yield details of the a

93 plicable to the rapidly increasing number of cryoEM density maps of macromolecular assemblies.

94 op' resource for deposition and retrieval of cryoEM maps, models and associated metadata.

95 manuscript demonstrate that single particle cryoEM is capable of competing with X-ray crystallograph

96 ve examined the potential of single-particle cryoEM for determining the structure of influenza-virus

97 mined at 2.8 A resolution by single-particle cryoEM.

98 We performed cryoEM-guided molecular dynamics flexible fitting simula

99 ng the C-terminal half of SCP and performing cryoEM reconstruction, we demonstrate that SCP's N-termi

100 Here we present cryoEM structures of two Hsp104 variants in both crossli

101 fibrils is markedly different from previous cryoEM models of Abeta(1-40) fibrils.

102 Recent cryoEM density maps of Abeta(42) fibrils obtained at low

103 A recent cryoEM structure of yeast CMG shows that duplex DNA ente

104 ally, were modelled into the 28 A resolution cryoEM map of the procapsid.

105 Using near-atomic resolution cryoEM reconstruction and single filament TIRF microscop

106 Here, we report the near-atomic resolution cryoEM structures of the Escherichia coli AcrAB-TolC mul

107 apsid proteins in our near-atomic-resolution cryoEM map of the grass carp reovirus virion, a member o

108 he ongoing interpretation of high resolution cryoEM and x-ray electron density maps.

109 g from, that inferred from a high resolution cryoEM structure of a triskelion in a clathrin basket.

110 c models based on medium- to high-resolution cryoEM density maps.

111 These high-resolution cryoEM structures have clarified important domains not p

112 lographic structures) with medium-resolution cryoEM densities.

113 techniques with the subnanometer-resolution cryoEM structure of rotavirus, we now provide a more det

114 s capsid-binding properties using NMR, SAXS, cryoEM and SPR.

115 MOTIF-EM takes cryoEM volumetric maps as inputs.

116 The cryoEM density map also reveals few, weak interactions b

117 The cryoEM image reconstruction permits a nearly complete tr

118 The cryoEM structure of FcRY at pH 6 revealed a compact doub

119 The cryoEM structure of the FcRY-IgY revealed symmetric bind

120 The cryoEM structure, sequence comparison, and protein fold

121 of isolated C-linker/CNBD fragments and the cryoEM structures of related CNG, HCN, and KCNH channels

122 gh-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosec

123 the resulting capsid, which was shown by the cryoEM study to closely resemble the infectious mature v

124 We determined the cryoEM structure of CypA in complex with the assembled H

125 antibody to block fusion, we determined the cryoEM structures of the C10-ZIKV complex at pH levels m

126 The structural features revealed from the cryoEM map lead to a juxtaposed stacking model of choles

127 y subtracting a pseudoatomic capsid from the cryoEM reconstruction.

128 rientation between helix 9 segments from the cryoEM study, the solid state NMR data lead to a unique

129 cated localizes the N terminus of SCP in the cryoEM density map and enables us to construct a pseudoa

130 NMR models for Abeta(17-42) fit well in the cryoEM density map and reveal that the juxtaposed protof

131 nterface at the local three-fold axis in the cryoEM map and confirmed its functional importance by mu

132 trimeric surface protrusions observed in the cryoEM map.

133 ntacts made between GluA2 TMD and Stg in the cryoEM structures.

134 ing of the Fab and virus structures into the cryoEM densities identified the footprints of each antib

135 and penton base crystal structures into the cryoEM density established that alpha-helices of 10 or m

136 This model was fitted into the cryoEM density for each of the 25 trimeric CIV capsomers

137 er, the resulting model fits better into the cryoEM density map than the initial template structure.

138 h viruses and of ICAM-1 were fitted into the cryoEM density maps.

139 l domains D1 and D2 has been fitted into the cryoEM density of the complex.

140 3)Sigma3(3) heterohexameric complex into the cryoEM image of an intact virion, reveal molecular event

141 The overall fit of the L1 model into the cryoEM map is excellent, but residues 402-446 in the 'C-

142 logy model for the MCP upper domain into the cryoEM map reveals that SCP binds MCP largely via hydrop

143 Analysis of the cryoEM density was enhanced by docking the crystal struc

144 Comparison of the cryoEM pore complex to the prepore structure obtained by

145 , giving us an independent validation of the cryoEM results.The two structures also augment our under

146 Comparison of the cryoEM structure with bent and extended models for the i

147 Based on the cryoEM measurements and on NMR data, we probe amyloid fi

148 for medaka and human is modeled based on the cryoEM structure of Tetrahymena telomerase, providing in

149 Here we report the cryoEM structure of a coronavirus S glycoprotein in the

150 Here, we report the cryoEM structure of yeast U1 snRNP at 3.6 A resolution w

151 ded into domains to obtain a good fit to the cryoEM density.

152 Using the cryoEM method MicroED, we discover that one segment, 19-

153 Using the cryoEM method microelectron diffraction, we determined t

154 Tomographic cryoEM images of the cell division site show separate co

155 Cryoelectron microscope tomography (cryoEM) and a fluorescence loss in photobleaching (FLIP)

156 Here we used cryoEM to determine the structure of the intact LliK CNG

157 re to approximately 10-A resolution by using cryoEM and the iterative real-space reconstruction metho

158 ing local structures of protein models using cryoEM maps as a constraint.

159 he reduced LDL receptor was visualized using cryoEM; reduced LDL receptors showed images with a diffu