ライフサイエンスコーパス: cryopreserve

1 cultured continuously for decades and can be cryopreserved.

2 iral vector encoding GM-CSF, irradiated, and cryopreserved.

3 iral vector encoding GM-CSF, irradiated, and cryopreserved.

4 iabetes, and Schwann cells were isolated and cryopreserved.

5 transferred, but not by whether embryos were cryopreserved.

6 ryos were transferred and extra embryos were cryopreserved.

7 varied by age and whether extra embryos were cryopreserved.

8 yte antigen (HLA)-matched related donors and cryopreserved.

9 male germline stem cells can be successfully cryopreserved.

10 Ewes' left ovary was removed and cryopreserved.

11 transplantation, from which 11 embryos were cryopreserved.

12 use of a larger number of oocytes or embryos cryopreserved.

13 indicator tube culture, 1 mL with Xpert, and cryopreserved 0.5 mL, later tested with Xpert Ultra.

14 Cryopreserved 1-mg/mL aliquots of alteplase are safe and

15 patients who had ovarian tissue successfully cryopreserved, 14 were available for assessment of ovari

16 iming with G-CSF and were either immediately cryopreserved (48 patients; cohorts A and B) or were fir

17 ly plan how many straws are needed to safely cryopreserve a genetic line.

18 -Hodgkin lymphoma and targeted sequencing on cryopreserved aliquots of autologous stem-cell products

19 Cryopreserved aliquots of P. multocida were administered

20 ation may improve the in vivo performance of cryopreserved allogeneic heart valves.

21 The ability to cryopreserve alteplase aliquots makes it an economically

22 s used to analyze synaptosomes prepared from cryopreserved Alzheimer's disease tissue.

23 pose of this randomized study is to evaluate cryopreserved amniotic membrane (CAM) for helping cicatr

24 Also, the use of cryopreserved amniotic membrane allows surgeons to perfo

25 ng degenerated Tenon's and reconstruction by cryopreserved amniotic membrane improved the recovery ra

26 emicity (in Ghana, Guinea, and Senegal) were cryopreserved and cultured ex vivo after thawing in a si

27 ic of hemangioblasts, and could be expanded, cryopreserved and differentiated into multiple hematopoi

28 The hematopoietic potential of cryopreserved and ex vivo manipulated umbilical cord blo

29 titative RT-PCR in independent datasets from cryopreserved and formalin-fixed paraffin-embedded sampl

30 multiple units of platelets, which could be cryopreserved and reinfused to counteract severe thrombo

31 Adult OB core neurospheres were cryopreserved and subsequently cultured.

32 ine serum (standard media) and compared with cryopreserved and thawed tissue.

33 Comparison with two controls, one cryopreserved and the other kept as the experimental pop

34 Spermatozoa from a number of species can be cryopreserved and then subsequently used to fertilize eg

35 Cell lines could be isolated and cryopreserved and, upon subsequent thawing, continue to

36 Culture-derived (CD) B cells are readily cryopreserved and, when recovered, retain their ability

37 A need to confirm that the cryopreserving and thawing of the reconstituted solution

38 0-selected cells could be clonally expanded, cryopreserved, and multiplied for over 50 days in adhere

39 ECs were then chemically selected, expanded, cryopreserved, and re-expanded in culture.

40 , enriched ex vivo by CD34+ immunoselection, cryopreserved, and reinfused after treatment with cyclop

41 Tissues were cryopreserved, and streptavidin alkaline phosphatase imm

42 ansferred and whether extra embryos had been cryopreserved, and they were high compared with those of

43 nt have been time tested: mechanical valves, cryopreserved aortic homograft, stented heterograft, and

44 We evaluated recovery of functional HPC cryopreserved as mononuclear or unseparated cells for up

45 nd cultured in the M-SFM for up to 2 months, cryopreserved at -70 degrees C within 1-3 days of isolat

46 ne profiles were compared with donor samples cryopreserved at transplant and beyond 10 years from SCT

47 (CCIs) between the 19 paired transfusions of cryopreserved autologous platelets and fresh allogeneic

48 esponses in an antigen-unbiased way, we used cryopreserved autologous pretreatment blood samples (rep

49 ophosphamide (60-100 mg/kg), and infusion of cryopreserved autologous stem cells.

50 subjects had > or = 1 x 10(6) CD34+ cells/kg cryopreserved based on both actual and target weights, a

51 subjects had > or = 2 x 10(6) CD34+ cells/kg cryopreserved based on the target weight.

52 clear cells requires 2-3 h, and cells may be cryopreserved before transplantation.

53 hematopoietic stem cells were collected and cryopreserved before treatment with [(131)I]MIBG greater

54 a novel approach that allows testing LTP in cryopreserved brain.

55 d the allogeneic T-cell response elicited by cryopreserved c-kit-selected hCPC.

56 Cryopreserved CACs but not EPCs retained functional acti

57 Of the 11 patients that received cryopreserved cadaveric allografts no deaths were report

58 dy response attesting to the antigenicity of cryopreserved cadaveric allografts.

59 Cryopreserved cadaveric venous or arterial allografts ar

60 are made on a segment that was attached to a cryopreserved CBU.

61 ed with the recipient and contained a median cryopreserved cell dose of 3.7 x 107 (range, 1.6 x 107-6

62 Cryopreserved cell monolayers are a new cell culture tec

63 The results indicated that fresh and cryopreserved cell monolayers are comparable in sensitiv

64 Two cryopreserved cell monolayers, ELVIS for the detection o

65 aracterization of the initial, expanded, and cryopreserved cell products confirmed maintenance of SC

66 ples and methods used for the preparation of cryopreserved cell stocks.

67 dherent cell culture, but current methods to cryopreserve cells in this format can give low cell yiel

68 or large-scale production, reconstitution of cryopreserved cells for clinical use, defining cell pote

69 Robust results were obtained from cryopreserved cells prepared by both strategies, yieldin

70 > or =0.005%), quantitative, feasible using cryopreserved cells, and able to define epitope specific

71 mma interferon (IFN-gamma) Elispot assays on cryopreserved cells.

72 eq, the assay is successful with slow-cooled cryopreserved cells.

73 lizing and quantifying the ultrastructure of cryopreserved cells.

74 The ex vivo production of cryopreserved cellular products for use in tissue repair

75 d 19 cytokines/chemokines were measured from cryopreserved cerebrospinal fluid (CSF) and serum during

76 culture, have normal karyotypes, and can be cryopreserved, clonally isolated, and stably transfected

77 ortant ground-work principles for the use of cryopreserved coral sperm for future reef restoration ef

78 lution X-ray diffraction data collected from cryopreserved crystals, and a new structure of the compl

79 Fresh and cryopreserved donor cells were compared for their abilit

80 nts with cancer have achieved pregnancy with cryopreserved eggs.

81 uterine laparoscopic technique with fresh or cryopreserved electroejaculates from asymptomatic males

82 hES) cell lines in xeno-free conditions from cryopreserved embryos.

83 potent hematopoietic progenitors that can be cryopreserved, enriched by physical methods, and expande

84 akaryocyte progenitor cell lines that can be cryopreserved, expanded in vitro, and induced to undergo

85 Cryopreserved eyes from 18 diabetic and 9 nondiabetic su

86 kidney transplant on day 0, then infusion of cryopreserved FCRx on day +1.

87 ved iliac vein, n=37; and Group 3, cadaveric cryopreserved femoral vein, n=11.

88 If venous conduits must be utilized, cryopreserved femoral veins seem to provide superior pat

89 Two tissue specimens were cryopreserved for >1 month before successful generation

90 The second half of the pheresed product was cryopreserved for delayed administration.

91 cultures have been successfully passaged and cryopreserved for future study.

92 Hepatocytes are cryopreserved for later use and this is detrimental to m

93 CD34(+) cells isolated from CB cryopreserved for up to 21 years had long-term (>/= 6 mo

94 Ovarian tissue was cryopreserved from a 30-year-old woman with breast cance

95 PV) and hepatic artery (HA) complications of cryopreserved grafts in each patient.

96 Dimethylsulfoxide (DMSO) is used to cryopreserve hematopoietic stem cells and is obligatoril

97 Patients with cryopreserved hematopoietic stem cells (n = 148) were tr

98 ts had MIBG-positive lesions and tumor-free, cryopreserved hematopoietic stem cells.

99 stant neuroblastoma, (131)I-MIBG uptake, and cryopreserved hematopoietic stem cells.

100 The aim of this study was to assess cryopreserved hepatocyte proliferative capacity in vivo

101 The freshly isolated and cryopreserved hepatocytes conserved the protein levels o

102 These findings suggest that cryopreserved hepatocytes represent an appropriate sourc

103 s, and patient received 2.18x10(9) fresh and cryopreserved hepatocytes through a Hickman line inserte

104 Banking of cryopreserved hepatocytes would fulfill the latter requi

105 Patient 1 received a total of 1.09x10(9) cryopreserved hepatocytes, and patient received 2.18x10(

106 of mattress hiPSC-CMs were consistent across cryopreserved hiPSC-CMs generated independently at anoth

107 We used composite valved conduits made from cryopreserved homograft and polytetrafluoroethylene (PTF

108 Cryopreserved homograft valves are an imperfect but sati

109 dies have explored the long-term function of cryopreserved homograft valves used for reconstruction o

110 on's practice, an evolution away from use of cryopreserved homografts for LVOT reconstructions except

111 Among 205 patients receiving cryopreserved homografts for reconstruction of the RVOT

112 The use of cryopreserved homografts has improved reconstruction of

113 odels (3xTg and Tg2576) and, importantly, in cryopreserved human AD brain samples.

114 Cryopreserved human amniotic membrane, stained with Rose

115 ds, we prepared synaptosomes from samples of cryopreserved human association cortex, and immunolabele

116 rovirus receptor (amphoR) mRNA were found in cryopreserved human cord blood HSC.

117 rescent protein (eGFP) was used to transduce cryopreserved human corneal endothelial cells.

118 Most hES cell lines are derived from cryopreserved human embryos that were created during in

119 Cryopreserved human heart valves are used in approximate

120 led trial of transplanted isolated fresh and cryopreserved human hepatocytes as a bridge to orthotopi

121 plasminogen activator) mice, freshly thawed cryopreserved human hepatocytes were transduced with a l

122 Quantitative microculture assays of cryopreserved human immunodeficiency virus type 1-infect

123 isease (SCID) mouse model, culture-expanded, cryopreserved human MSCs have osteogenic potential and d

124 odontal ligament to test the hypothesis that cryopreserved human periodontal ligament contains retrie

125 post-natal stem cells can be recovered from cryopreserved human periodontal ligament, thereby provid

126 of eGFP is highly efficient and nontoxic to cryopreserved human primary cultured corneal endothelial

127 ne the viability and potential usefulness of cryopreserved human primary cultured corneal endothelial

128 It is not known whether cryopreserved human tissues contain functional post-nata

129 experience, we recommend avoiding the use of cryopreserved iliac vein for portal vein reconstruction

130 m the living donor, n=18; Group 2, cadaveric cryopreserved iliac vein, n=37; and Group 3, cadaveric c

131 (CSV) as arterial conduits led us to develop cryopreserved iliac veins (CIV) as interposition grafts

132 l analysis, these results discourage the use cryopreserved iliac veins for portal interposition and c

133 rapy might avoid this fate by engraftment of cryopreserved immature testicular tissue after therapy i

134 human spermatogonia after xenotransplant of cryopreserved immature testicular tissue into immunocomp

135 ified that epigenetic results from fresh and cryopreserved iMNs quantitatively agree.

136 went autologous transplants with stem cells, cryopreserved in 10% DMSO (vol/vol).

137 All collections were cryopreserved in 5% dimethyl sulfoxide and 6% hydroxyeth

138 hepatocytes (viability, 97.4% +/- 0.9%) were cryopreserved in 60 micromol/L ZVAD-fmk (+ZVAD group) or

139 Autologous tumor-free bone marrow was cryopreserved in all patients receiving 12 mCi/kg and mo

140 Cells were cryopreserved in diverse sample matrices, namely, phosph

141 10-14 days when split at a 1:3 ratio and is cryopreserved in liquid nitrogen.

142 Cells of different passages were cryopreserved in medium containing dimethyl sulfoxide an

143 Fresh rat hepatocytes were cryopreserved in suspension in culture media (Media-cryo

144 tic mosaicism was demonstrated on leukocytes cryopreserved in the first patient when he was 22 years

145 Harvested platelets were cryopreserved in ThromboSol and 2% dimethyl sulfoxide (D

146 in cultures of porcine hepatocytes that were cryopreserved in ZVAD-fmk.

147 The angiogenesis process in cryopreserved islet grafts required a longer time period

148 We have developed a method to cryopreserve islets with excellent survival of endocrine

149 by intradermal or intravenous injection with cryopreserved, isogenic NF54 P. falciparum sporozoites (

150 important step forward to the utilization of cryopreserved isolated hepatocytes in bioartificial live

151 l mPTP modulators, a method was exploited to cryopreserve large batches of functionally active mitoch

152 ata are presented showing that a screen of a cryopreserved library of clonal, germ line competent, N-

153 erall and regardless of whether embryos were cryopreserved, live-birth rates increased if more than 2

154 Using cryopreserved lymphocytes collected in an ongoing, clini

155 Serum, cerebrospinal fluid (CSF), and cryopreserved lymphocytes from subjects in the Rush Alzh

156 sphamide 75 mg/kg, followed by reinfusion of cryopreserved marrow.

157 sh an initial perspective on the quantity of cryopreserved material needed to ensure success in prese

158 microscale human liver platform composed of cryopreserved, micropatterned human primary hepatocytes

159 Ca(2+)-induced mitochondrial swelling in the cryopreserved mitochondria identified ER-000444793, a po

160 The cryopreserved mitochondria maintained respiratory coupli

161 image infected cells and the viral particles cryopreserved next to them.

162 ect venous inoculation of aseptic, purified, cryopreserved, non-irradiated PfSPZ ('PfSPZ Challenge')

163 Cryopreserved nonvalved allografts induce a strong HLA a

164 dissected endoneurial microvessels from four cryopreserved normal adult human sural nerves referenced

165 enchymal cells, which can be administered as cryopreserved off the shelf products, seem to be promisi

166 Cryopreserved organisms were thawed and injected intrave

167 erve fertility before these treatments is to cryopreserve ovarian tissue for later transplantation.

168 ivebirth after orthotopic transplantation of cryopreserved ovarian tissue and the continued developme

169 Reimplantation of cryopreserved ovarian tissue has been shown to restore n

170 nd sets the stage for the transplantation of cryopreserved ovarian tissue in humans.

171 re than 35 babies have been born so far with cryopreserved ovarian tissue in patients with cancer who

172 inoculation (DVI) of 3200 aseptic, purified, cryopreserved P falciparum sporozoites (PfSPZ Challenge;

173 f novel ex vivo protocols to study fresh and cryopreserved P vivax (n = 135) and P falciparum (n = 77

174 y by isolating Ag-reactive plasmablasts from cryopreserved PBMC obtained from volunteers vaccinated w

175 nsitivity of detection of CD8 responses from cryopreserved PBMC was achieved using autologous vaccini

176 Cryopreserved PBMCs were obtained from cancer patients b

177 Cryopreserved PBPC caused less aggregation than fresh PB

178 PC and at least 5 x 10(6) CD34(+) uncultured cryopreserved PBPC per kilogram.

179 Fresh and cryopreserved PBPC were evaluated as well as PBPC obtain

180 On day 0, the cryopreserved PBPCs were reinfused and on day +1 the 12-

181 and 2 hours after the onset of reperfusion, cryopreserved performed as well as fresh MSC to rescue r

182 These cryopreserved periodontal ligament stem cells maintained

183 Cryopreserved peripheral blood mononuclear cells (PBMC)

184 In retrospective studies performed on cryopreserved peripheral blood mononuclear cells (PBMCs)

185 Cryopreserved peripheral blood mononuclear cells and ser

186 Cryopreserved peripheral blood mononuclear cells were us

187 surgical resection, prospectively collected cryopreserved peripheral-blood lymphocyte specimens (n =

188 of aseptic, purified, radiation-attenuated, cryopreserved PfSPZ ('PfSPZ Vaccine'); or by infectious

189 --composed of attenuated, aseptic, purified, cryopreserved PfSPZ--was safe and well tolerated when ad

190 scriptase (RT) and protease sequencing using cryopreserved plasma aliquots obtained from 46 heavily t

191 tat and/or gag sequencing of HIV-1 from cryopreserved plasma samples could be performed on 15 of

192 We measured concentrations of L-arginine in cryopreserved plasma samples from Tanzanian children wit

193 e transcriptase polymerase chain reaction on cryopreserved plasma samples, previously obtained CD4+ l

194 Levels of biomarkers in cryopreserved plasma were measured by enzyme-linked immu

195 olled human malaria infections (CHMIs) using cryopreserved Plasmodium falciparum sporozoites provide

196 et count <15,000/microL) and then autologous cryopreserved platelets at the next, or to group B (n=10

197 Transfusion of autologous cryopreserved platelets derived from recombinant human t

198 Cryopreserved porcine hepatocytes are a ready source of

199 Furthermore, when compared with cryopreserved preparations, early function (postoperativ

200 Cryopreserved, pretransplant bone marrow, peripheral blo

201 ial livers (HEALs) stabilize the function of cryopreserved primary human hepatocytes through juxtacri

202 Cryopreserved, primary, cultured human corneal endotheli

203 probable benefit, CD154+TcM were measured in cryopreserved samples from 214 children younger than 21

204 ble CD163 [sCD163] level) were measured from cryopreserved samples from the Study to Understand the N

205 Reports of early success with cryopreserved saphenous veins (CSV) as arterial conduits

206 ved iliac veins for portal interposition and cryopreserved saphenous veins for arterial interposition

207 Immunohistochemistry was performed on cryopreserved sections of embryonic/fetal human eyes fro

208 al microscopy and by immunohistochemistry of cryopreserved sections of eyes of albino and pigmented m

209 None of the six inseminates with thawed cryopreserved semen resulted in infection.

210 We measured serum CC16 concentrations in cryopreserved serum samples and reviewed vital status up

211 Cryopreserved serum samples from these patients were use

212 We evaluated the morphology of cryopreserved sheep ovarian cortex grafted in the presen

213 Using cryopreserved specimens from a prior study of pericardit

214 erapy on fertility, although men may wish to cryopreserve sperm prior to treatment.

215 - and larger-scale reproduction trials using cryopreserved sperm (p < 0.05); 3) cryopreservation did

216 st settlement for coral larvae produced from cryopreserved sperm and established important ground-wor

217 nt success, as larvae produced with fresh or cryopreserved sperm had the same settlement success (p >

218 acaques were inoculated intravenously with a cryopreserved stock of microglia obtained from a simian

219 ion about mutant mouse lines held as live or cryopreserved stocks at MRC Harwell.

220 arried out autologous orthotopic grafting of cryopreserved testicular tissue from four prepubescent m

221 levels in murine serum that were higher for cryopreserved than for freshly isolated hBTSCs.

222 nic stem cell (ESC) line can be repetitively cryopreserved, thawed, expanded, and differentiated into

223 dle biopsies, and can generate cultures from cryopreserved tissue and from fewer than four viable cel

224 In six patients cryopreserved tissue and in nine patients fresh tissue p

225 used with cells derived from both fresh and cryopreserved tissue samples.

226 ere greater for SFM-cultured islets than for cryopreserved tissue.

227 After IVF, all resultant embryos were cryopreserved to preserve fertility.

228 Among women who had extra embryos cryopreserved, transferring more than two embryos increa

229 ates of intrinsic clearance determined using cryopreserved trout hepatocytes can be extrapolated to t

230 The results of this study demonstrate that cryopreserved trout hepatocytes can be used to reliably

231 ducting substrate depletion experiments with cryopreserved trout hepatocytes from a single source.

232 Cryopreserved tumor tissue sections were immunostained f

233 (+) T regulatory cell (Treg) enrichment from cryopreserved UCB followed by a 18 (+) 1-day expansion c

234 Therefore, we used cryopreserved umbilical cord blood (UCB) as a strategy t

235 We conclude that cryopreserved umbilical cord blood from HLA-matched and

236 es 1" compared the effects of freshly thawed cryopreserved umbilical cord-mesenchymal stem/stromal ce

237 "Series 2" examined the effects of cryopreserved umbilical cord-mesenchymal stem/stromal ce

238 h intact envelopes were observed for samples cryopreserved under different conditions, which was inco

239 cation has greatly improved the potential to cryopreserve unfertilised oocytes.

240 entially extend the pool of suitable donors, cryopreserved unrelated donor umbilical cord blood was c

241 e-mobilized, collected by leukapheresis, and cryopreserved using 5% dimethyl sulfoxide and 6% hydroxy

242 y demonstrates that isolated rat hepatocytes cryopreserved using HTS showed high viability, long-term

243 Cryopreserved valved allografts in children induce a mar

244 The HLA immunogenicity of cryopreserved valved allografts used in the surgical rep

245 eart transplantation in patients who receive cryopreserved valved allografts.

246 ugh December 31, 1994, one surgeon implanted cryopreserved valved homografts into 149 patients--65 si

247 as observed in both syngeneic and allogeneic cryopreserved valves relative to fresh valves.

248 present, 11 LRLT have been performed without cryopreserved vein conduits over 17 mo with no vascular

249 vein stenosis was associated most often with cryopreserved vein for portal conduits.

250 results, retrospective analysis of long-term cryopreserved vein graft performance in LRLT at our inst

251 Cryopreserved versus freshly isolated hBTSCs were equall

252 Cryopreserved versus freshly isolated hBTSCs were simila

253 ant increase in the phase angle theta of the cryopreserved vessels (theta=24.30+/-6.32; P<0.001) comp

254 statistical analysis of results obtained for cryopreserved vessels to the fresh vessels showed signif

255 Recently, a commercially available human cryopreserved viable amniotic membrane (hCVAM) has been

256 Microvascular transplantation of cryopreserved whole ovary may allow immediate revascular

257 (more than one billion cells per batch) and cryopreserved with good viability.

258 r the first time that engineered NPSC can be cryopreserved with no loss of their basic properties, th

259 blood progenitor cells (PBPCs) are commonly cryopreserved with the cryoprotectant dimethyl sulfoxide

260 ny healthy children have been born from eggs cryopreserved with the Kuwayama egg vitrification techni

261 rease in insulin content of grafts from ICCs cryopreserved with trehalose compared with ICCs cryopres

262 Grafts from islets cryopreserved with trehalose contained 14-fold more insu

263 d, expanded to approxiamtely 10(7) cells and cryopreserved within 6-8 weeks.

264 opreserved with trehalose compared with ICCs cryopreserved without trehalose.

265 14-fold more insulin than grafts from islets cryopreserved without trehalose.

266 Our results demonstrate that freshly thawed cryopreserved xeno-free human umbilical cord-mesenchymal