ライフサイエンスコーパス: culture

1 whereby negative specimens are excluded from culture.

2 or of persuasive messages, social media, and culture.

3 bed the need to 'fit in' with organisational culture.

4 ntiated in vitro, failed to redecidualize in culture.

5 he cellular microenvironment in primary cell culture.

6 rmal cells isolated from them grow slowly in culture.

7 us cells and primary dopaminergic neurons in culture.

8 the outgrowth of aneuploid clones in tissue culture.

9 ed with reduced viability and growth in cell culture.

10 wth factor beta to set up a dual-compartment culture.

11 ion zone of the lower lip (TZ) after six-day culture.

12 y 20% reduction in yield from induced sputum culture.

13 us patterning systems in vivo and in explant culture.

14 (6-6000muM) was mixed with fat explants and cultured.

15 F21 gene transcription in primary hepatocyte cultures.

16 spinal neuron co-cultures and astrocyte-only cultures.

17 We recapitulated these results in cell cultures.

18 ell motility and increased colony size in 2D cultures.

19 is in normal and benign but not in carcinoma cultures.

20 nonical WNT signaling in these SOX2-enriched cultures.

21 ublicly available transcriptomes of neuronal cultures.

22 mp in response to Bmp6 in primary hepatocyte cultures.

23 of drusen-like deposits in patient hiPSC-RPE cultures.

24 were not detected by the LFD in Spanish MGIT cultures.

25 cal review and collection of blood and stool cultures.

26 on-canonical Wnt5a in pericyte but not in EC cultures.

27 carbon (serine)-limited continuous chemostat cultures.

28 y of neonatal rat ventricular myocyte (NRVM) cultures.

29 TJP expression in the Mtb-stimulated BBB co-cultures.

30 ratio [OR] 1.6, 95% CI 1.3-1.9, p<0.0001) or culture (1.7, 1.2-2.4, p=0.01).

31 5 mL with mycobacteria growth indicator tube culture, 1 mL with Xpert, and cryopreserved 0.5 mL, late

32 A total of 252 blood cultures, 126 in each group, were included in the final

33 been derived from naturally occurring, cell culture-adapted, or genetically modified live attenuated

34 ces between these reticulocytes and in vitro-cultured adult reticulocytes functionally or at the mole

35 described can be repeatedly performed during culture, allowing for real-time, longitudinal analysis o

36 ould be monitoring for contamination in pure cultures; analysis of mixed bacterial cultures, where ex

37 nt, to also include impacts on human health, culture and biodiversity conservation more generally.

38 d Krox20 are dispensable for adipogenesis in culture and for brown adipose tissue development in mice

39 e viruses evolved during replication in cell culture and in experimentally infected macaques.

40 ow that stem-like activity in serial passage culture and in vivo breast morphogenesis relies on the p

41 Using in vitro cell culture and in vivo mouse models, we showed that COUP-TF

42 ro data, along with those from previous cell culture and in vivo studies by others, suggest that l-Gl

43 and Krox20 are required for adipogenesis in culture and in vivo Using conditional knockout mice and

44 onstrate that RPE degeneration in human-cell-culture and mouse models is driven by a noncanonical-inf

45 ith the decrease of CHK1 levels both in cell culture and mouse rhadomyosarcoma xenografts.

46 Experience with human iPS cell culture and sorting via FACS will be of benefit for rese

47 ntentious distinction would improve both the culture and the effectiveness of the scientific process,

48 ient, requires additional operation for cell culture and therefore, is not compatible with point-of-c

49 il 2013) microbiology laboratories underwent culture and whole-genome sequencing (WGS), using WGS to

50 Colonic explants were cultured and preserved ex vivo for 35 days and co-cultur

51 ancer-99), which activates autophagy in cell cultures and animal models.

52 opy of media from astrocyte-spinal neuron co-cultures and astrocyte-only cultures.

53 dded by extracting RNA from independent cell cultures and degrading particular samples.

54 They have been used as remedies in many cultures and have been reported to provide beneficial he

55 re beginning to supplant traditional 2D cell cultures and preclinical animal studies that have histor

56 We infected nonhuman primate cell cultures and then crab-eating macaques with either simia

57 ally suited to connect people from different cultures and thereby foster mutual understanding.

58 ype of biosorbent (whether consortia or pure cultures), and the type of metal.

59 r toxigenic C. difficile by direct toxigenic culture, and 141 of 682 subjects were positive by using

60 esis were higher in coculture than in axenic culture, and this was reflected in increased amounts of

61 ndothelial cells were successfully isolated, cultured, and expanded from eight 20-mm, 18-gauge hepati

62 to placement and containment, environmental culturing, and disinfection.

63 cal and evolutionary theory, but also treats culture as more than a proximate mechanism that is direc

64 mouse primary keratinocytes in conventional cultures as determined by the nuclear Smad2/3 localizati

65 o investigate the metabolic effects of NO in cultured astrocytes from mice by taking advantage of the

66 he activity of Cx43 hemichannels recorded in cultured astrocytes was [Ca(2+)]I dependent.

67 rains of newborn and adult infected rats and cultured astroglioma cells, shunting tryptophan degradat

68 ing bacterial strain typing, immunization of cultures, autoimmunity or self-targeted cell killing, an

69 Culture-based blood-brain barrier (BBB) models are cruci

70 The current gold standard, culture-based diagnostics, can provide clinicians with c

71 icians an average of 9 days sooner than with culture-based DST for first-line drugs and 32 days soone

72 stematic, standardised surveillance of blood culture-based febrile illness in 13 African sentinel sit

73 es and determined to shed atypical EPEC at a culture-based prevalence of 18%.

74 mococcal if either sputum Gram stain, sputum culture, blood culture, or the immunochromatographic (IC

75 Finally, in a cortical neuron primary culture, both Nanobodies were able to inhibit endogenous

76 n as evidenced by the optical density of the culture broth.

77 n = 117), and specimens with negative fungal culture, but with microscopic and ancillary findings ind

78 uctures is extensively studied in suspension cultures, but remains poorly understood in substrate-dep

79 The disruption of autolysis in B. subtilis cultures by TiO2 NPs suggests the mechanisms and kinetic

80 st, we developed methods to more effectively culture C. auris from patients and their environment.

81 , we sequenced the genomes of two additional cultured Ca.

82 ifferent changes in the lipid composition of cultured CD11c(+) cells, and highlights the important ro

83 studying the effect of anti-cancer drugs in cultured cell lines by monitoring phosphatidylserine tra

84 iption (TPRT) and mobilized efficiently in a cultured cell retrotransposition assay.

85 HSV infection in these cultured cells shows the properties expected for a laten

86 ibe key findings in human postmortem brains, cultured cells, and animal models of disease that suppor

87 Experiments in cultured cells, brain slices, and in living mice demonst

88 ts Nf1 RasGAP activity in vivo as it does in cultured cells.

89 In this study, we demonstrate that culturing cells in different physical environments, stif

90 These effects can be recovered by culturing cells in the presence of a ROS quencher or in

91 the data center of the World Federation for Culture Collections (WFCC)-Microbial Resource Center (MI

92 antibiotic-treated and germ-free mice, using cultured commensals from the Actinobacteria, Bacteroidet

93 Upon in vitro culture, compared to the GFP group, cells from BMP group

94 ts indicate that EPCR is a reliable and cell culture-compatible marker of UM171-expanded human cord b

95 ht into functional differences that exist in culture conditions and among ebolavirus glycoproteins.

96 Under standard culture conditions BMP4 acts as a morphogen but this req

97 Furthermore, we describe culture conditions to maintain mitochondrial-depleted ce

98 The impact of culture conditions was further seen when inhibitory effe

99 In common culture conditions, we found that small molecule inhibit

100 Xi) of primed hESCs was reactivated in naive culture conditions.

101 ompared to cells cultured in standard tissue culture conditions.

102 AML cells in BM stroma-derived and standard culture conditions.

103 quires TCTP in MCF-7 cells under normal cell culture conditions.

104 Of 12 culture-confirmed pulmonary tuberculosis cases identifie

105 The conditions for culturing confluent monolayers on the sensor slides were

106 ociated with a significant decrease in blood culture contamination in patients undergoing blood cultu

107 nth sputa with MTBDRplus can predict 2-month culture conversion and long-term treatment outcome.

108 vitreoretinal findings, treatment regimens, culture data, and final visual acuities.

109 vivo and in cell lines and primary neuronal culture derived from timed pregnant rats in vitro, resul

110 In this study, we use organotypic cultures derived from transgenic mice inducibly expressi

111 hods for inducing Wnt signaling in stem cell cultures do not control the spatial presentation of the

112 nduced scratching behavior and activation of cultured dorsal root ganglion neurons from mice.

113 In cultured dorsal spinal neurons, blockade of Kv3.4 by blo

114 ulted in attenuation of virus growth in cell culture due to a reduction in assembly of mature virions

115 ents ecotypic differentiation and has defied culturing efforts so far.

116 In stimulated versus unstimulated organoid cultures, elevated IFN-gamma reduced the mRNAs encoding

117 n pregnant women with primary infection, the cultured ELISPOT assay detected a higher T-cell response

118 Determination of HCMV-specific T cells by cultured ELISPOT, in pregnant women with primary HCMV in

119 solated intestinal crypts from C57BL/6 mice, cultured enteroids, incubated these with TNF (50 ng/mL,

120 However, in the embryo and in defined culture environments the properties of pluripotent cells

121 In productively infected neuronal cultures, epinephrine treatment significantly increased

122 ricts pluripotent developmental potential in cultured ESCs and iPSCs.

123 Further in vitro cell culture experiments and gene expression analysis reveale

124 om by personnel with standard mammalian cell culture expertise.

125 Exposure of cultured fibroblasts to uniaxial cyclic stretch results

126 lope was assessed with immunofluorescence on cultured fibroblasts.

127 TP release was attenuated in Calhm1 knockout cultures following mechanical stimulation at a pressure

128 e width of 10mus after the treatment of 20-h culture for 10min, the maximum accumulation of both ions

129 ensity encapsulation to normoxic or ischemic culture for 12 hours, after which viability and function

130 ells from wild-type and STAT6-deficient mice cultured for 4 d in the presence or absence of IL-4.

131 rs of happiness, food, and physical activity culture from geolocated Twitter data to examine the rela

132 The prevalence of potential pathogens cultured from induced sputum specimens and quantity of o

133 Mast cells were cultured from peripheral blood CD34(+) cells and examine

134 ly, Notch2 activation in osteoblast-enriched cultures from Notch2(COIN) mice induced Tnfsf11 expressi

135 mens for detecting fungi; microscopy, fungal culture, galactomannan antigen, and aspergillus PCR are

136 te the transcriptome of primary monolayer KC cultures grown from lesional (PP) and non-lesional (PN)

137 However, it has been shown that liquid-culture-grown Arabidopsis can take up and store palladiu

138 In cultured H9c2 myoblasts, pharmacological inhibition of c

139 cation of microorganisms from positive blood cultures has improved clinical management and antimicrob

140 We exposed human bronchial epithelial cultures (HBECs) to air or whole tobacco smoke from ciga

141 IL-1beta-induced IL-6 and IL-8 secretion in cultured HGF and HPLF.

142 ecialised laboratory testing, in addition to culture, histopathology, and imaging expertise.

143 in the lungs of infected mice but not within cultured host cells, which indicates LegC4 augments bact

144 The protocol requires skills for culturing hPSCs and careful attention to morphological c

145 in nucleoli and co-localizes with R-loops in cultured human cells.

146 ss-link-containing plasmid was replicated in cultured human cells.

147 rease (by 1,000-fold) TNF gene expression in cultured human LAD2 and primary mast cells derived from

148 y expressed genes (DEGs), but analysis of KC cultures identified more PP- and PN-decreased DEGs.

149 technique for bacterial identification after culture in anaerobic and aerobic conditions.

150 of autoimmunity and Th17-skewing human cell culture in vitro.

151 We find that cells cultured in adult bovine serum, which better reflects nu

152 Conversely, when strain PC574 was cultured in human plasma, no similar increase in hemolyt

153 erm LIF-independent self-renewal of ES cells cultured in media containing fetal bovine serum (FBS) an

154 e on glutamine anaplerosis compared to cells cultured in standard tissue culture conditions.

155 In addition, H9C2 rat cardiomyocytes were cultured in vitro and the phosphorylation of ERK1/2, AKT

156 e contamination in patients undergoing blood cultures in an Emergency Department setting.

157 ced demyelination model on organotypic slice culture, in a BDNF-dependent manner.

158 euronal mammalian cells, in primary neuronal culture, in brain slices of mouse and monkey, and in mou

159 dentifying and addressing sources of bias in culture, including technology.

160 We then compared these culture-independent genomes to existing genomes of bacte

161 e of prions from infected to naive astrocyte cultures, indicating the generation of prion infectivity

162 r data demonstrates that extensive long-term culture-induced MSC aging impaired their osteogenic abil

163 r testing, while approximately 60% performed culture interpretation.

164 Early microbiological cure on culture is a predictor of clinical response to treatment

165 nce those laboratories would need to perform culture isolation prior to typing.

166 ng a 70% Caco2/30% HT-29 human intestinal co-culture layer.

167 In neuronal cultures lithium attenuates iron efflux by lowering tau

168 In cultured macrophages, recombinant CTRP6 dose-dependently

169 lture medium, fast-growing adventitious root cultures may hold promise as a sustainable resource for

170 mented for monitoring variations in CHO cell culture media upon exposure to high temperature short ti

171 whether supplementation of antioxidants in a culture medium could protect immature mouse oocytes from

172 Lactate levels in the culture medium starting from 50microM with production ra

173 mic conditions nor the absence of insulin in culture medium were sufficient to promote cell death.

174 Supplementation of culture medium with Neu5Ac stimulated expression of IL-6

175 ignificant enrichment of diterpenoids in the culture medium, fast-growing adventitious root cultures

176 g the combined direct and enriched toxigenic culture method (reference method), for a prevalence rate

177 ome this limitation, we developed an ex vivo culture method of the mammary gland where the direct act

178 Improvements in stem cell culture methods, materials and biophysical tools that as

179 In human intestinal organ cultures, microbial activation of Vgamma9/Vdelta2 T cell

180 mine whether vascular smooth muscle cells in cultured microvascular networks maintain the ability to

181 s the importance of a three-dimensional (3D) culture model including these cell types for investigati

182 Using an in vitro culture model yielding human mo-DCs and mo-Macs closely

183 miRNAs by dampening their expression in cell culture models and HCV-infected human livers.

184 for applicable multicellular tumor spheroid culture models and recent studies related to their appli

185 be used to characterize the response of cell culture models to perturbations such as pharmacologic mo

186 By combining in vivo and tissue culture models, we show here that VEGF165-induced vascul

187 Increased Malat1 levels were found in cultured mouse BMECs after OGD as well as in isolated ce

188 included 1 participant with endophthalmitis (culture negative), 9 with IOP more than 10 mm Hg greater

189 Defining the microbial etiology of culture-negative prosthetic joint infection (PJI) can be

190 Addition of purified C3 cleavage products to cultured neurons suggested that C3b is responsible for t

191 Exposure of cultured neurons to fetal plasma or to secretions from t

192 The pathway was confirmed by using cultured neurons treated with recombinant TNFalpha in vi

193 ll reprogramming, we generated a stable cell culture of an extremely rare and aggressive neuroendocri

194 ection rate was significantly enhanced by co-culture of leukocytes with cell lines prior to molecular

195 Culture of multiple periprosthetic tissue samples is the

196 of IL-13-induced lung pathology and in vitro culture of murine fibroblast cell lines and primary fibr

197 However, in vitro culture of neurons deprives them of their natural enviro

198 vice based on inertial sorting for perfusion culture of suspended mammalian cells.

199 ere determined in air-liquid interface (ALI) cultures of control and asthmatic primary human bronchia

200 Here we report that cultures of expanded potential stem cells can be establi

201 Primary cultures of hepatocytes derived from wild-type or hepato

202 )-mediated signaling was assessed in primary cultures of Kupffer cells from ethanol- and pair-fed rat

203 n cell identity, we established synchronized cultures of mouse embryonic stem cells as they exit the

204 d from infected individuals or from in vitro cultures of P. falciparum, making them prone to high var

205 The cultures of Saccharomyces cerevisiae were treated with p

206 Using in vitro cultures of several cell types found in the heart, we de

207 t MCAK promotes fast MT growth speeds in ECs cultured on compliant 2D ECMs but promotes slow MT growt

208 Ms but promotes slow MT growth speeds in ECs cultured on compliant 3D ECMs, and these effects are myo

209 al cells (HCECs) and human keratocytes (HKs) cultured on the optimal hybrid construct both demonstrat

210 Dependence of cell cultures on "whole serum" must be examined carefully alo

211 uced apoptosis in the presence of stromal co-culture or cytoprotective survival signals.

212 Fungal culture or histopathology confirmed Cryptococcus infecti

213 deficient TH2 cells were studied in in vitro culture or in vivo after challenge of Spi2A knockout mic

214 ents with fungal infections who had positive culture or longer duration of the disease.

215 HA digestion in wild type NSC cultures or in the SGZ induces increased NSC proliferati

216 ce Interval [CI] 1.51-5.03), people-oriented culture (OR=2.59, 95% CI 1.45-4.62), and ergonomic pract

217 her sputum Gram stain, sputum culture, blood culture, or the immunochromatographic (ICT) BinaxNow S.

218 tial suspicion criteria using antibiotic and culture order combinations in terms of patient character

219 bacterial, eukaryotic and viral genomes from cultured organisms, (ii) single cell genomes (SCG) and g

220 tor genes and taste transduction elements in cultured organoids.

221 than PEf1 in infecting E. coli K-12 in pure cultures, PEf1 was 20-fold more effective in suppressing

222 to spoligotyping of acid-fast-positive MGIT cultures, percent agreement between positive LFD and spo

223 t induction periods and using various tissue culture plates.

224 ustrial scale with a standard mammalian cell culture platform and a routine purification protocol.

225 727 distinct HAIs, of which 331 (45.5%) were culture positive.

226 issions with presumptive PTB, 20 (6.3%) were culture-positive for Mycobacterium tuberculosis.

227 The proportions of participants with culture-positive PTB initiated on appropriate TB treatme

228 6 per 0.10 decrease; 95% CI, 1.04-1.30), CSF culture positivity (HR, 1.37; 95% CI, 1.02-1.84), and bl

229 e strongly associated with poor outcome (CSF culture positivity, CSF white blood cell count, hemoglob

230 6%), where spoligotyping was applied to MGIT cultures previously confirmed to be positive for M. tube

231 hnique for determining membrane mechanics of cultured primary afferent neurons of the dorsal root gan

232 phoresis and stable isotope labeling in cell culture proteomics that the TbSTT3A and TbSTT3B proteins

233 The prevalence of culture-proven UTI among pregnant women with UTI symptom

234 ated uptake of fluorescently labeled NEFA in cultured proximal tubule cells and microperfused rat pro

235 Short-term exposure of cultured rat hippocampal neurons or ex vivo human cortic

236 were screened for toxigenic C. difficile by culturing rectal swabs.

237 ear expression induces profound autophagy in cultured renal epithelial cells.

238 come measures were positive donor rim fungal culture results and the development of postkeratoplasty

239 In addition, IL-7-stimulated pro-B cell cultures revealed a reduced differentiation from large p

240 exposure to IL-2, or by addition of IL-12 to cultures, revealing that cytokine signaling could restor

241 an be easily implemented in a typical tissue culture room by personnel with standard mammalian cell c

242 Most strikingly, phagocytosis of POS by cultured RPE cells was almost completely blocked by phar

243 placement was concordant between direct and cultured samples.

244 In this study, we introduce pre-culture SBB treatment to suppress autofluorescence, wher

245 to the CA1 area of the mouse hippocampus in cultured slices, acute slices and in vivo.

246 We cultured soil from a rice field in Laos for B. pseudomal

247 In our case, early recognition, culture-specific intravenous antibiotics and urgent surg

248 Here, using cultured spinal cord (SC) neurons grown using a compartm

249 Of all known cultured stem cell types, pluripotent stem cells (PSCs)

250 We developed an efficient cell culture system and isolated HEV particles that were infe

251 We used an organotypic culture system of human fetal testes explants called FEt

252 sion pathway, we used an ex vivo erythrocyte culture system to decrease expression of GPA, GPB, or GP

253 We further utilize an improved CD34+ culture system to engineer human red blood cells that ex

254 tment or vaccine for the disease and no cell culture system to propagate the virus.

255 In this study, a co-culture system was developed for innervation of intrafus

256 their expected effects, irrespective of the culture system, IWP2 decreased total beta-catenin while

257 In our in vitro T cell culture system, MART1-specific CD8 T cells were expanded

258 scribed a novel, near-physiological organoid culture system, wherein primary human healthy liver cell

259 In co-culture systems, MFs secreted high levels of IL-6, while

260 finding contradicted observations from cell culture systems.

261 utionary neuroscience approach to cumulative culture, taking into account experimental, developmental

262 in the pursuit of an evolutionary science of culture that is grounded in both biological and evolutio

263 Interestingly, cultures that received DCM and CM together degraded both

264 After 1 week of culture, the c-Kit(+) population is further enriched by

265 As they progress in culture, the FLI-matured cumulus-oocyte complexes displa

266 Unlike cells in culture, the physiological fate of cells that die by apo

267 GS underwent transplantation with allogeneic cultured thymus.

268 We used three-dimensional tissue culture to build an organotypic model of bronchial dyspl

269 tance of organizational safety practices and culture to promote safe work practices for patient handl

270 rimary lens epithelial cells within the same culture to undergo differentiation into either lens fibe

271 Rplus was performed on mycobacteria-positive cultures to ascertain acquired drug resistance (ADR).

272 used steady-state oxic and anoxic chemostat cultures to demonstrate that the switch from aerobic to

273 All the strains were grown in culture under the same experimental conditions and ident

274 period with the EOS calculator period, blood culture use decreased from 14.5% to 4.9% (adjusted diffe

275 ee areas, thus providing information on cell culture viability, cellular mechanisms and multicellular

276 e of the Na-/K-ATPase inhibition in the cell culture was demonstrated by the corresponding alteration

277 , we observed that prion replication in cell culture was inversely related to the levels of expressio

278 whether patients who had a positive repeated culture was predictive of worse clinical outcome than th

279 and propane-1,3-diol in cheese and bacterial cultures was developed.

280 red and preserved ex vivo for 35 days and co-culturing was performed with C. parvum.

281 In neurons in culture we showed that IGF-1 receptor activation is impo

282 TNTg(+)Tlr4(-/-) mice, and human immune cell culture, we demonstrate that hRetn binds the LPS recepto

283 abolic changes in astrocyte-spinal neuron co-cultures, we carried out metabolomic analysis by (1)H NM

284 e approach for recovering axenic Fibrobacter cultures, we isolated 45 novel strains from 11 different

285 measurements of p21 protein in proliferating cultures, we show that naturally occurring DNA damage in

286 Primary enteric glial cultures were generated from the VillinCre:Men1(FL/FL):S

287 Among these, 200 monomicrobial cultures were included in the comparative analysis.

288 Clinical cultures were included, with the first CRKP isolate reco

289 whereas an additional 34% were unclear since cultures were negative in one of the hospitalizations.

290 When cultures were negative, the antibiotic treatment was sto

291 ve antibiotic for at least 2 days when blood cultures were taken, and subsequent episodes in the same

292 ually any DNA sequence, particularly in cell culture where selection can be used to recover relativel

293 n pure cultures; analysis of mixed bacterial cultures, where examining one species in the presence of

294 rexpression might cause cytotoxicity in NRVM cultures, which could be alleviated without impairing el

295 and accumulated lipids in the cytoplasm when cultured with butyric acid, a principal short-chain fatt

296 ecrete IgG and increase CD27 expression when cultured with soluble piperacillin.

297 In comparison, the NO2(-) spiked cultures with COD:N = 4:1 showed significantly higher (p

298 Neural progenitor cell (NPC) culture within three-dimensional (3D) hydrogels is an at

299 viability, induced necroptosis, and delayed culture wound closing in three types of immortalized can

300 ere associated with a 45% reduction in blood culture yield and approximately 20% reduction in yield f