ライフサイエンスコーパス: culture system

1 allenges the physiological relevance of this culture system.

2 en hampered by the lack of an efficient cell culture system.

3 d macrophages (J771.A1) using a Transwell co-culture system.

4 yonic stem cells in a three-dimensional (3D) culture system.

5 l, using a feeder-free and serum-free (FFSF) culture system.

6 re flagged as positive by an automated blood culture system.

7 ly responds to activated macrophages in a co-culture system.

8 e migration in two-and-a-half-dimensional/3D culture system.

9 DSPP-null and wild-type mice in an in vitro culture system.

10 quired HCV infection and (ii) in an HCV cell culture system.

11 and D subtype viruses by >200-fold in a cell culture system.

12 anism using a persistently HCV-infected cell culture system.

13 several cytochrome P450 (CYP) genes in this culture system.

14 hepatoblasts is insufficient in the present culture system.

15 on strategy in a primary murine erythroblast culture system.

16 ferent than those of the Myco/F lytic liquid culture system.

17 d to achieve this goal, we developed a novel culture system.

18 ral stem-cell-derived three-dimensional (3D) culture system.

19 have been hampered due to the lack of a cell culture system.

20 rence-mediated gene silencing in an HCV cell culture system.

21 containing PF medium in the BacT/Alert blood culture system.

22 s between adipocytes and macrophages in a co-culture system.

23 u pathology in a single 3D human neural cell culture system.

24 ed and maintained under this novel Ff and Xf culture system.

25 T/Alert (bioMerieux, Inc., Durham, NC) blood culture system.

26 adipogenesis in a human primary preadipocyte culture system.

27 f breast cancer cells in a three-dimensional culture system.

28 ion and protein expression in an HCV J6/JFH1 culture system.

29 polysaccharide (LPS)-activated BV2 microglia culture system.

30 esponse to an EF in a two-dimensional and 3D culture system.

31 ted cells in a two-chamber trans-well tissue culture system.

32 in an ex vivo bone marrow-derived eosinophil culture system.

33 significantly impairs myelination in our co-culture system.

34 by the cleavage event, at least in the cell culture system.

35 atients and are also produced in an in vitro culture system.

36 mentally in a novel parasite-erythrocytes co-culture system.

37 in a self-assembling, primary hepatocyte co-culture system.

38 n hampered by the lack of an efficient viral culture system.

39 laboratory because of limitations in the HCV culture system.

40 o induce bone resorption in an ex vivo organ culture system.

41 ghbors but inhibited by grass neighbor in co-culture system.

42 finding contradicted observations from cell culture systems.

43 nal (2D) and organoid three-dimensional (3D) culture systems.

44 and most studies have been confined to cell culture systems.

45 d to these large, complex, and heterogeneous culture systems.

46 o yeast were not detected by automated blood culture systems.

47 level of cell death that occurs in all cell culture systems.

48 studies also employing murine and human cell culture systems.

49 differences were observed between the two co-culture systems.

50 tly enhance the infectivity of HIV-1 in cell culture systems.

51 ion of a vast array of data obtained with DC culture systems.

52 oduces dose-dependent SOD1 reduction in cell culture systems.

53 causing mutations and Ca(2+) signaling in 2D culture systems.

54 lity not possible with conventional 2D or 3D culture systems.

55 d research into treatment has relied on cell culture systems.

56 ne can be used in long-term (8 wk) 3D tissue culture systems.

57 n of the limitations of air-liquid interface culture systems.

58 t liver tissue compared with two-dimensional culture systems.

59 ing is still reliant on conventional 2D cell culture systems.

60 the development of versatile and permissive culture systems.

61 n SMA model mice and human motor neuron cell culture systems.

62 n signaling modification is dependent on the culture systems.

63 ic inferences remains to be tested in tissue culture systems.

64 ious surveys did not use insect tissues as a culturing system.

65 ive blood cultures than a conventional blood culture system (12.1 h versus 14.9 h, P < 0.001) while a

66 Hence, ex vivo organ culture systems allow pre-screening and pre-validation o

67 We will also explore how this new culture system allows disease modeling and gene repair f

68 cluding the development of an efficient cell culture system and animal models for HBV investigation,

69 We developed a cell culture system and characterized HEV particles; we ident

70 Here, we present a novel microfluidic co-culture system and establish mild, moderate and severe c

71 we used a purified rat retinal ganglion cell culture system and found that hUTCs secrete factors that

72 We developed an efficient cell culture system and isolated HEV particles that were infe

73 for assembly, we utilized an embryonic bone culture system and pharmacologic inhibition of (DYRK) ki

74 re we outline here is applicable to any cell culture system and requires approximately 1 week to comp

75 Due to lack of an efficient cell culture system and robust small-animal model, little is

76 n ability of ESCC cells in three-dimensional culture systems and angiogenesis assay.

77 ly understood and the lack of efficient cell culture systems and animal models are the principal limi

78 in intestinal organoid-based mouse and human culture systems and augmented glucose-stimulated GLP-1 s

79 odels for SAC regulation developed in tissue culture systems and demonstrate that several fundamental

80 and possible solutions offered by novel cell culture systems and genome engineering approaches.

81 yte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomim

82 platform has been applied to both 2D and 3D culture systems and readily distinguishes between (1) cy

83 ivate human PPARgamma1 in a transfected cell culture system, and further research is needed to identi

84 rypt development using the in vitro organoid culture system, and illustrate a clear differential requ

85 ed and maintained in vitro using an adherent culture system, and the biological properties were compa

86 We discuss here how genetics, new cell culture systems, and improved animal models will fuel th

87 rmone receptor (GnRHR) have efficacy in cell culture systems, and their cellular and biochemical mech

88 Several three-dimensional cell culture systems are currently available to create liver

89 mics within these microcolonies, new sessile culture systems are needed that sequester cells and mimi

90 bute to tumor progression and establish this culture system as a platform for studying tumor vascular

91 antly greater growth benefit to PSCs in a co-culture system as compared with the MYB-silenced cells.

92 proliferation and osteoclast differentiation culture systems, as well as in mouse models, for the res

93 l of the human liver, there are currently no culture systems available that sustain hepatocyte replic

94 We deployed an in vitro co-culture system based on direct contact of cancer cells w

95 blood progenitors, with a new and effective culture system being used for the human cells that gener

96 In co-culture systems, BMFs secreted high levels of murine int

97 In the C2C12 cell culture system, BMP-2-induced Smad 1/5/8 phosphorylation

98 enin and promotes cell-cell adhesion in cell culture systems, but its importance has not been demonst

99 We establish a continuous in vitro culture system by using human blood enriched for young c

100 We conclude that our 3D culture system can generate large numbers of fully funct

101 This ex vivo culture system can uniquely address the ability of CNS p

102 Highly efficient HCV full-length 2b culture systems can be established by using consensus cl

103 Thereby, organ culture systems can reduce animal use, and improve the s

104 This primary cell-based co-culture system combined with microOCT imaging offers sig

105 iciencies is the lack of controlled in vitro culture systems comprised of honey bee cells.

106 A co-culture system consisting of a polarized mucosal epithel

107 In human TH1- or TH2-skewing cell culture systems, cotranscriptional R-loops (RNA/DNA dupl

108 e first primary human macrophage-enteroid co-culture system, defined conditions that allow for a prac

109 The 3D cell culture system developed on a deformable substrate affix

110 The protocol relies on culture systems devoid of serum, feeders or complex extr

111 In contrast, the transwell co-culture system displays comparatively low levels of BBB

112 In both physiological and cell culture systems, EGF-stimulated ERK activity occurs in d

113 n this study, we engineered a scaffold-based culture system enabling brain endothelial cells to form

114 ver, studies in infectious strain JFH1-based culture systems expressing patient-derived Core-NS2 prot

115 Using novel 2a, 2b, and 2c cell-culture systems, expressing authentic envelope proteins,

116 Previously, we identified and established a culture system for a novel lineage of arenaviruses isola

117 Here we describe an in vitro primary culture system for Caenorhabditis elegans germline stem

118 Our findings validate a unique BCSC culture system for drug screening and offer preclinical

119 Development of a biomimetic 3D culture system for drug screening is necessary to fully

120 Together, these findings identify a cell culture system for functionally exploring the two X chro

121 we have developed a polarized 2-dimensional culture system for HAT-7 cells, a rat cell line of amelo

122 Due largely to the lack of an efficient cell culture system for HEV, the molecular mechanisms of HEV

123 ain-containing receptor--and an efficient 2D culture system for hiPS cell-derived endothelial precurs

124 nd good manufacturing practice-compatible 3D culture system for hPSC expansion and differentiation.

125 sis demonstrates the utility of the HCV cell culture system for identifying novel bioactive molecules

126 Using an organ culture system for prostate development and Ret mutant m

127 tion, thereby providing a more physiological culture system for studying integrin-ECM interactions in

128 compared to the ELVIS HSV ID and D(3) Typing Culture System for the qualitative detection and differe

129 We describe the first cell culture system for VA1, a key step necessary for the stu

130 evelopment and homeostasis, we used in vitro culture systems for both primary mouse myoblasts and C2C

131 This work describes cell culture systems for production of infectious HCV particl

132 We developed efficient culture systems for the epidemiologically important geno

133 In primary cell culture systems, forskolin not only down-regulated UPEC-

134 d be generated under feeder-free (Ff) and Xf culture systems from human primary fibroblasts and blood

135 Since the conventional culture system has some issues that need to be addressed

136 to society, yet the lack of reliable tissue culture systems has hampered the development of appropri

137 etic tools, imaging technologies and ex vivo culture systems has provided significant insight into th

138 yomavirus and cytomegalovirus, in which cell culture systems have accidentally provided unique potent

139 Recently, ex vivo three-dimensional organ culture systems have emerged to study the physiology and

140 gical improvements in three-dimensional (3D) culture systems have enabled the generation of organoids

141 nducted in humans, animal models, and tissue culture systems have enhanced our understanding of the m

142 Recent advances in 3D culture systems have led to the generation of brain orga

143 Current three-dimensional mammary culture systems have not demonstrated concurrent prolife

144 d with hepatitis C virus and in the HCV cell culture system (HCVcc), it is known that highly infectio

145 panese fulminant hepatitis-1 (JFH1) HCV cell culture system (HCVcc).

146 ly been performed with the use of viral cell culture systems; however, these tests are laborious and

147 t in an osteoblast/bone marrow macrophage co-culture system, immobilization of OPG by HS at the osteo

148 ted a high-efficiency, inducible cell fusion culture system in the normally nonfusing Drosophila S2R+

149 stem cell lines represent the first organoid culture system in the rat.

150 This protocol describes a culture system in which inner-ear sensory tissue is prod

151 Here we describe a novel culture system in which primary human HSPCs cultured und

152 ir advantages compared to traditional static culture systems in terms of high control of microenviron

153 e-inactivating studies in zebrafish and cell culture systems in vitro, we show that Par3 to be essent

154 uch of their biology has been elucidated via culture systems in which hematopoietic precursors differ

155 nderstudied due to a lack of sufficient cell culture systems in which to propagate the virus.

156 argely dependent on the availability of cell culture systems in which viruses can be propagated to in

157 an in vivo mouse model and in vitro explant culture system, in which an oncogenic allele of K-ras is

158 Furthermore, we used different types of culture systems, including co-culture, indirect co-cultu

159 in adult prefrontal cortex and various cell culture systems, including neurons derived from reprogra

160 Alert Virtuo is an advanced, automated blood culture system incorporating improved automation and an

161 Data from more advanced 3D culture systems indicate that this intrinsic propensity

162 o extend traditional 2-dimensional (2D) cell culture systems into 3D to more accurately replicate in

163 However, established co-culture systems involve brain endothelial cells, astrocy

164 The alginate scaffold-based organotypic culture system is a promising, reliable, and easy system

165 that the concentration of substances in the culture system is uniform.

166 their expected effects, irrespective of the culture system, IWP2 decreased total beta-catenin while

167 In our in vitro T cell culture system, MART1-specific CD8 T cells were expanded

168 The Myco/F lytic culture system may be an alternative to the MGIT system

169 Our study demonstrates that microfluidic culture systems may offer an interesting new tool for di

170 In co-culture systems, MFs secreted high levels of IL-6, while

171 Our culture system mimics the three-dimensional cellular str

172 ession profiles derived from common in vitro culture systems (monolayer and three-dimensional culture

173 T 116 spheroids are a three-dimensional cell culture system, morphologically and phenotypically simil

174 We used an organotypic culture system of human fetal testes explants called FEt

175 Metabolic profiling of cells in 2D culture systems often fails to reflect the metabolism oc

176 ome assembly, epidemiologic screening, and a culture system or animal model of infection are necessar

177 mpossible because there are no reported cell culture systems or in vivo models that support VA1 infec

178 g principles in stem cell biology, including culture systems, preclinical models, and functional asse

179 cal study demonstrated that the Virtuo blood culture system produced results comparable to those seen

180 cheal differentiated primary epithelial cell culture system provides a valuable in vitro model for st

181 lates for tissue formation) and bioreactors (culture systems providing environmental control).

182 The development of in vitro culture systems quantitatively and qualitatively recapit

183 (muXg) using the NASA developed rotary cell culture system (RCCS) enhanced bone resorbing osteoclast

184 This culture system recapitulates the human intestinal epithe

185 In a defined cell culture system recapitulating the extracellular matrix r

186 val of soluble IL-6R using a dynamically fed culture system, reduces mature myeloid cell production,

187 We found that an OP9-assisted culture system reinforced by addition of VEGF-A, VEGF-C,

188 Cell culture systems reproducing virus replication can serve

189 nhibition of this pathway in a whole oviduct culture system resulted in a decreased embryo transport

190 an behave as a subtractive compartmentalised culture system retaining and allowing maintenance of the

191 lysis of spheroid invasion in an organotypic culture system revealed that these "trailblazer" cells a

192 This de novo cell fusion culture system reveals a general role for actin-propelle

193 Our culture system should facilitate the study of human inne

194 ated our experimental design on a model cell culture system showing high sensitivity and specificity,

195 We applied 2 culture system techniques on human minor salivary gland

196 portantly, by exploiting a unique epithelial culture system that allowed us to monitor alterations in

197 e used these survival stimuli to establish a culture system that allows efficient infection of B and

198 ish a self-assembling, primary hepatocyte co-culture system that can be infected with patient-derived

199 However, there is no in vitro culture system that can capture the massive proliferatio

200 rimarily due to the lack of an in vitro cell culture system that demonstrates a deficit in replicatio

201 erformed functional studies using an ex vivo culture system that enriches for terminally differentiat

202 reported the characterization of a novel 3D culture system that exhibits key events in AD pathogenes

203 ese findings identify a robust in vitro cell culture system that is amenable to addressing mechanisms

204 We present a new nanoliter-scale sessile culture system that is easily implemented via microfluid

205 interactions in the gut would benefit from a culture system that maintained tissue architecture yet a

206 In an in vitro two-dimensional culture system that mimics the architecture of the intes

207 Here, we developed a robust culture system that permitted expansion and genetic mani

208 ent has been hampered by lack of an in vitro culture system that recapitulates in vivo DC hematopoies

209 In this report, we use an in vitro culture system that recapitulates the in vivo developmen

210 Here we report a culture system that supports development of CD34(+) hema

211 Here, we describe a 3D culture system that supports long-term expansion of prim

212 Using a tissue culture system that supports the development of human DC

213 d treatment of HBV requires an in vitro cell-culture system that supports the infection of human hepa

214 Here, we describe a three-dimensional culture system that supports the long-term growth and ex

215 By developing a culture system that sustains survival, we show that CD4(

216 We describe a 3D erythroid culture system that utilises a porous polyurethane (PU)

217 We devised a microfabricated organ culture system that viably preserves the normal multicel

218 A recent study reports a novel organ culture system that will enhance our ability to dissect

219 Using compartmentalized culture systems that isolate axons and nascent synapses,

220 ype-specific, with higher activity in T-cell culture systems that model the natural target cells for

221 The development of cell culture systems that provide accurate and physiologicall

222 -acting antiviral (DAA) agents in infectious culture systems that test the effects on different virus

223 Indeed, in the bacteria co-culture system, the host cells experience an altered env

224 Due to the lack of an efficient cell culture system, the molecular mechanisms of HEV infectio

225 In a minimal, serum-free culture system, the synthetic GC dexamethasone (DEX) was

226 Unlike in vitro culture systems, the cellular organization, compartmenta

227 numerous studies on mechanics using 2D cell culture systems, the spatial and temporal variations in

228 In a purified, defined culture system, these physiologic SCN ligands were suffi

229 igh resolution lineage tracing method and 3D culture system to analyse ADM in human cells.

230 To this end, we designed a simple, versatile culture system to control the location of nutrient deliv

231 sion pathway, we used an ex vivo erythrocyte culture system to decrease expression of GPA, GPB, or GP

232 We also used a neonatal rat cardiomyocyte culture system to elucidate the mechanisms underlying th

233 We further utilize an improved CD34+ culture system to engineer human red blood cells that ex

234 In addition, we use this in vitro culture system to expand human stress erythroid progenit

235 We have developed an ex vivo culture system to expand peripheral blood CD34(+) progen

236 Here, we used an organoid culture system to investigate how transcription and the

237 report the development of a robust in vitro culture system to produce RBCs that allow the generation

238 been well studied due to the lack of a cell culture system to propagate the virus.

239 tment or vaccine for the disease and no cell culture system to propagate the virus.

240 In this study, we employed a microfluidic co-culture system to recreate important interactions in the

241 In the present study, we used an HCV cell culture system to screen an uncharacterized chemical lib

242 In this study, we used an in vitro organ culture system to show that progesterone receptor membra

243 We have designed an in vitro culture system to specifically address the impact of ant

244 Here we use a novel tissue culture system to study a signaling pathway that control

245 which are the most physiologically relevant culture system to study drug metabolism in vitro, were u

246 We developed a reliable culture system to study SKs in vitro and screened these

247 We developed an in vitro culture system to study the ability of macrophages to in

248 d the newly approved BacT/Alert Virtuo blood culture system to the BacT/Alert 3D system using 115 cli

249 Here we extend this culture system to the propagation of primary liver cance

250 rr2 in different sepsis models, we used cell culture systems to evaluate inflammatory cytokine produc

251 Here, we utilize several human neuronal culture systems to investigate the role of one such MAPK

252 Availability of the new culture systems to study interactions between sensory ne

253 tocol can be easily applied to existing cell culture systems to study the complete HBV life cycle, in

254 eric addition in single neonatal CMs in a 3D culture system under acute, uniaxial, static, sustained

255 This study compares several adipogenic cell culture systems under a variety of conditions to assess

256 s was studied in an in vitro 24-hour hypoxic culture system using quantitative polymerase chain react

257 ) and can be analyzed similarly to the other culture systems using techniques such as immunohistochem

258 In this study, a co-culture system was developed for innervation of intrafus

259 tion on leaf-to-root transport, a soil-based culture system was developed to monitor root system arch

260 ated ErbB3 expression in the high-density 3D culture system was strongly associated with hypoxia-indu

261 A key component of the cell culture system was the use of a feeder cell line that wa

262 ts of HIV infection on Th17 and Th1 cells, a culture system was used in which CCR6(+) CD4(+) T cells

263 This culture system was used to identify a bacterial folate a

264 PDGF-BB in vasculogenesis in the 3D MM/UB co-culture system was validated by direct interference with

265 The cell culture system we have developed has potential applicati

266 Using this culture system, we defined the pathway for human DC deve

267 l cord neuron-oligodendrocyte myelinating co-culture system, we demonstrate that disruption of dynami

268 By using a three-dimensional culture system, we demonstrate that the inverse relation

269 Importantly, using an ex vivo human organ culture system, we demonstrate the feasibility of human

270 ACM fate mapping mouse model and an in vitro culture system, we demonstrated that adult mouse cardiom

271 Using a multiplexed cell culture system, we find that switching between certain c

272 Using a co-culture system, we found that co-culture of QSG-7701 (hu

273 tion of our in vitro primary epithelial cell culture system, we found that prostaglandin E2 (PGE2) si

274 In a cell culture system, we found TNF to have antiviral effects i

275 l SMAD signaling inhibition in a feeder-free culture system, we have been able to expand airway basal

276 Here, using a hepatitis C virus (HCV) cell culture system, we identified neoechinulin B (NeoB), a f

277 eral proteins through an ex vivo reaggregate culture system, we identify BMPER as a novel positive re

278 Using a three-dimensional culture system, we modulate TGF, BMP, FGF, and WNT signa

279 Using a rapid, clinically compliant culture system, we show that autologous BK virus-specifi

280 ced complexity in vitro macrophage-T cell co-culture system, we show that macrophage arginase-1 is th

281 emically-defined, serum and feeder cell-free culture system, we show that the AhR is expressed in HPs

282 Using ELISA and AXIS co-culture systems, we demonstrate that C5aR is critically

283 wound healing assays, and three-dimensional culture systems, we identified a mother centriole subdis

284 Finally, using computer simulation and cell culture systems, we provide evidence for a role of MT nu

285 Lung fibrosis models and cell culture systems were employed.

286 In vitro primary sensory neuron culture systems were subjected to whole-transcriptome se

287 we used a noncontact neuronal-astrocytic co-culture system, where synthetic Abeta peptides were adde

288 These findings were extended to a cell culture system wherein loss of 4E-BP1 and 4E-BP2 resulte

289 scribed a novel, near-physiological organoid culture system, wherein primary human healthy liver cell

290 Unlike other human astrovirus cell culture systems, which require addition of exogenous try

291 , and thus attain accurate dosimetry in cell culture systems, which will greatly advance the developm

292 A successful, long-term, robust culture system will depend on a multifaceted approach co

293 Studies combining 2D and 3D cell culture systems will assist in understanding how mutatio

294 its native host, reverse genetics, and cell culture systems-will continue to provide important insig

295 Here, using an in vitro parasite culture system with erythrocytes from iron-deficient and

296 ombined a highly synchronous photobioreactor culture system with frequent temporal sampling to charac

297 Using a zebrafish embryo culture system with reporters of early and late skeletal

298 bryo patterning is established in species or culture systems with irregular cell divisions.

299 her summarizes the current state of HCV cell culture systems with respect to available virus isolates

300 In cell co-culture systems, YKL-40 enhanced both GSDC and HMVEC con