ライフサイエンスコーパス: cultured neuron

1 expression from hippocampal brain slices and cultured neurons.

2 e-3 activity in defined dendritic regions of cultured neurons.

3 unoreactive puncta in dendritic processes of cultured neurons.

4 of inhibitory synapses in mouse hippocampal cultured neurons.

5 in the rat nigro-striatal projection and in cultured neurons.

6 typically measured during quantal release at cultured neurons.

7 es and changes in the metabolite profiles of cultured neurons.

8 nd reduced spine density and size in primary cultured neurons.

9 etabolites that enhance neurite outgrowth in cultured neurons.

10 in exocytosis involved in neurite growth in cultured neurons.

11 also reduced death and apoptosis of infected cultured neurons.

12 ndrial and nuclear localization of RIbeta in cultured neurons.

13 t that AEA up-regulates Notch-1 signaling in cultured neurons.

14 media from these microglia promoted death of cultured neurons.

15 xic phenotype that causes the rapid death of cultured neurons.

16 ation of RNF167 is located on the surface of cultured neurons.

17 shRNA reduces excitatory synapse density in cultured neurons.

18 any aspect of the RIM knockout phenotype in cultured neurons.

19 actin cytoskeleton, in either HeLa cells or cultured neurons.

20 alpha4 subunit expression in hippocampus and cultured neurons.

21 lycans and participate in CSPG inhibition in cultured neurons.

22 domain and Dnmt3a RNAi blocked apoptosis of cultured neurons.

23 ranscriptional rhythms in brain explants and cultured neurons.

24 NMDA-mediated Ca(2+) influx and currents in cultured neurons.

25 ay important roles in synaptic plasticity in cultured neurons.

26 h rate in the neurites (but not the soma) of cultured neurons.

27 ation, growth, and retraction of neurites of cultured neurons.

28 organisms such as C. elegans and dissociated cultured neurons.

29 NMDA receptor stimulation (chemical LTD) in cultured neurons.

30 th Rab11a and Rab4 in presynaptic boutons of cultured neurons.

31 turned over and display minimal toxicity to cultured neurons.

32 their effects on mitochondrial morphology in cultured neurons.

33 sed [corrected] dendritic spine head size in cultured neurons.

34 ed miniature EPSC amplitude and frequency in cultured neurons.

35 on and trafficking of glutamate receptors in cultured neurons.

36 d activity in several cell lines and primary cultured neurons.

37 hibition by Nogo66, MAG, OMgp, and myelin in cultured neurons.

38 distribution of Kv2.2 protein within single cultured neurons.

39 cells and to promote neurite outgrowth from cultured neurons.

40 al role of TopIIbeta in neurite outgrowth in cultured neurons.

41 en shown to be cytotoxic when incubated with cultured neurons.

42 d inhibition of catecholamine secretion from cultured neurons.

43 nsional (2D) images of fluorescently labeled cultured neurons.

44 r Abeta-dependent Bim induction and death in cultured neurons.

45 monitor rapid trains of action potentials in cultured neurons.

46 boosting their neuroprotective action in co-cultured neurons.

47 ynchronous release, or vesicle repriming, in cultured neurons.

48 tivation of NMDA-type glutamate receptors in cultured neurons.

49 to shape illumination over single somata of cultured neurons.

50 10 muM, ZIP-induced excitotoxic death of the cultured neurons.

51 a signaling scaffold protein, AKAP79/150, in cultured neurons.

52 erely reduced in cultures in gp130-deficient cultured neurons.

53 multiple mammalian cell lines and in primary cultured neurons.

54 es that recycle actively or spontaneously in cultured neurons.

55 s glucocorticoid-induced MAO A expression in cultured neurons; 3) induction of KLF11 and translocatio

56 ly known as NR1) subunit of NMDAR causing in cultured neurons a selective and reversible internalizat

57 A) resulted in PTEN nuclear translocation in cultured neurons, a process requiring mono-ubiquitinatio

58 Furthermore, in primary cultured neurons, a proportion of UCH-L1(M) does partiti

59 o change artificial CSF (ACSF) [Na(+)](o) at cultured neurons accurately (SD<7 mM) and rapidly (<5s)

60 cellular cholesterol synthesis or content in cultured neurons affect the cleavage of amyloid precurso

61 a, and reduces Abeta production from primary cultured neurons after AAV2/1-FGF2 infection.

62 t is, kainic acid injection) and detected in cultured neurons after knockdown of Xpo1, the gene encod

63 ion of 17beta-estradiol (17betaE2) protected cultured neurons against amyloid toxicity, and its actio

64 N1 protected primary cultured neurons against toxicity and cell death trigger

65 that siRNA-mediated loss of calsyntenin-1 in cultured neurons alters APP processing to increase produ

66 Consistent with the results seen in cultured neurons, an extremely high rate of Abeta produc

67 penumbra induces autophagy and apoptosis in cultured neuron and astrocyte cell lines and that this m

68 We treated cultured neuron and astrocyte cell lines with IS for up

69 cantly reduced apoE4's neurotoxic effects in cultured neurons and a series that reduced apoE4 fragmen

70 In cultured neurons and acute brain slices, we found that m

71 Specifically, we found that, in cultured neurons and acute slices of the hippocampus, ex

72 In cultured neurons and acute slices of the olfactory bulb,

73 Both in cultured neurons and acute slices, KO of Rab11Fip5 had n

74 Using cultured neurons and an AD mouse model, we show for the

75 tion against DNA damage and neurotoxicity in cultured neurons and an in vivo model for ischemia.

76 stimulation of central neuronal activity in cultured neurons and Ang II-induced inhibition of barore

77 We tested GCaMP5s in several systems: cultured neurons and astrocytes, mouse retina, and in vi

78 In cultured neurons and brain slices, we show that Cal-Ligh

79 expression of wild-type and mutant Shank3 in cultured neurons and by binding assays in heterologous c

80 disrupted by activation of NMDA receptors in cultured neurons and by stimuli that trigger late-phase,

81 Assembled BoNT/X cleaves VAMP2 and VAMP4 in cultured neurons and causes flaccid paralysis in mice.

82 mbinant wild-type GABAAR endocytosis in both cultured neurons and COS-7 cells can be amplified by the

83 rotect against oxygen glucose deprivation in cultured neurons and did not alter the expression of LC3

84 e show that G2019S-LRRK2 expression, in both cultured neurons and dopaminergic neurons in the rat sub

85 onal protein synthesis is well recognized in cultured neurons and during development in vivo, there h

86 ssed a recombinant truncated Src fragment in cultured neurons and examined how it affects neuronal su

87 Similar vacuoles are found in cultured neurons and fibroblasts.

88 s in heterologous cells with GluN1 or in rat cultured neurons and found that surface expression corre

89 nt quantitative proximity ligation assays in cultured neurons and four independent immunoprecipitatio

90 Studies in cultured neurons and glia cells indicate that these chan

91 UPS activity, we found that UPS activity in cultured neurons and glia decreases in a time-dependent

92 irenz (EFV) alters mitochondrial function in cultured neurons and glial cells.

93 to Galphaolf and induce cAMP accumulation in cultured neurons and HEK-T cells.

94 In cultured neurons and hippocampal organotypic slices, syn

95 tude of inhibitory synaptic currents in both cultured neurons and hippocampal slices exposed to E2, w

96 Here we demonstrate, in cultured neurons and in acute slices, that the NMDA rece

97 induce a pathological modification of tau in cultured neurons and in bigenic mice expressing Abeta an

98 es aggregation of alpha-synuclein in primary cultured neurons and in dopaminergic neurons of the subs

99 We demonstrated in cultured neurons and in fruit fly and zebrafish larvae h

100 utant htt decreases synaptic UPS activity in cultured neurons and in HD mouse brains that express N-t

101 ccumulates and leads to neurodegeneration in cultured neurons and in mouse brain.

102 the EphrinB2 receptor in heterologous cells, cultured neurons and in mouse brain.

103 ized the performance of the new red GECIs in cultured neurons and in mouse, Drosophila, zebrafish and

104 ivity ("Optopatch") has been demonstrated in cultured neurons and in organotypic brain slices, but no

105 d sub-threshold membrane voltage dynamics in cultured neurons and in pyramidal cells within neocortic

106 pplication of glutamate receptor agonists in cultured neurons and in response to acoustic stimulation

107 ue 5-HT(1A) signaling of 5-HT(1A) KO mice in cultured neurons and in slices of the dorsal raphe showi

108 ecessary for its transcriptional activity in cultured neurons and in the developing brain in vivo We

109 of the endogenous biliverdin chromophore in cultured neurons and in the mammalian brain and can be r

110 lsion but not Sema3C-dependent attraction in cultured neurons and in the mouse brain.

111 of HSF1-dependent genes, Hsp70 and Hsp90, in cultured neurons and in the mouse cerebral cortex in viv

112 rospectively identifying growing synapses in cultured neurons and in visualizing the distribution of

113 regulates excitatory synapse development in cultured neurons and in vivo, and the synaptogenic activ

114 his chimeric motor rescued axon outgrowth in cultured neurons and in vivo, firmly establishing the ro

115 -GNTI) reduced the response of DPDPE both in cultured neurons and in vivo.

116 (GCaMP6) that outperformed other sensors in cultured neurons and in zebrafish, flies and mice in viv

117 that trigger excitatory synapse assembly in cultured neurons and influence synaptic function in vivo

118 ed synaptic loss and neuronal death in mouse cultured neurons and long-term potentiation inhibition i

119 In cultured neurons and mouse and fly brains, smFP probes a

120 In cultured neurons and nonneuronal cells, Lfc has been sho

121 Recording from cultured neurons and ON ganglion cells in the flat-mount

122 th genetic and pharmacological approaches in cultured neurons and PD patient-derived cells.

123 key regulator of life or death decisions in cultured neurons and sensory cells.

124 e analyses of their dendritic mRNA levels in cultured neurons and synaptoneurosomes did not detect di

125 at oligomeric Abeta enhances PLD activity in cultured neurons and that this stimulatory effect does n

126 otoxins in AD, impair organelle transport in cultured neurons and transgenic mouse models.

127 d to detect the enzyme in brain homogenates, cultured neurons, and histological sections.

128 Immunochemistry on rat brain, cultured neurons, and human embryonic kidney cells expre

129 receptors and impairing synaptic function in cultured neurons, and it prevented memory deficits and n

130 ses SMN expression in patient-derived cells, cultured neurons, and the mouse central nervous system.

131 ling was the primary cascade that attenuated cultured neuron apoptosis after growth factor withdrawal

132 Xenopus laevis growth cones from 4-8-h cultured neurons are attracted to BMP7 gradients but bec

133 nd that cytosolic proteins in axons of mouse cultured neurons are conveyed in a manner that superfici

134 response to focal ischemia in vivo and when cultured neurons are deprived of oxygen and glucose.

135 Catecholamine murine cultured neurons are more responsive to induction of MHC

136 Using synaptic vesicle aggregation in cultured neurons as a marker of presynaptic differentiat

137 Immunohistochemistry on rat brain and cultured neurons as well as cell-based assays were used

138 term imaging of synaptic vesicle dynamics in cultured neurons as well as in intact zebrafish.

139 d neuroprotection from glutamate toxicity in cultured neurons at 2 weeks in vitro.

140 rmore, LCBs induced neurite fragmentation in cultured neurons at concentrations corresponding to the

141 dative stress-mediated cell death in primary cultured neurons at nanomolar concentrations.

142 ikingly, addition of recombinant neurexin to cultured neurons at submicromolar concentrations induced

143 mpal slices, cortical synaptoneurosomes, and cultured neurons, BDNF-induced mTOR pathway activation a

144 Our results indicate that, in cultured neurons, both BDNF and EGF activate m-calpain b

145 tyrate (NaBu) induced PKCdelta expression in cultured neurons, brain slices, and animal models.

146 e slices or using a chemical LTD protocol in cultured neurons but did not affect hippocampal LTP.

147 Axonal mRNA transport is robust in cultured neurons but there has been limited evidence for

148 itic spine formation, growth and motility in cultured neurons, but its role in brain in vivo is unkno

149 ges in neuronal activity, is well studied in cultured neurons, but not in more physiological networks

150 been shown to block the A-type K+ current in cultured neurons, but their specificity has been questio

151 ith anti-VEGF antibody reduced the growth of cultured neurons by 17% and the regeneration of subbasal

152 A screen for transcripts robustly induced in cultured neurons by DNA damage identified Sertad1, a Cdk

153 substantially increased the excitability of cultured neurons by increasing the spontaneous firing ra

154 We found that, in Drosophila cultured neurons, Ca2+ currents were mediated predominan

155 JNK3 deficiency reduces degeneration of cultured neurons caused by low levels of SMN.

156 icromolar concentrations of C1ql proteins to cultured neurons causes a significant decrease in synaps

157 Axotomy-induced hyper-excitability of cultured neurons coincides with elimination of inhibitor

158 Immature glutamatergic synapses in cultured neurons contain high-release probability (Pr) p

159 ion with HSV was restricted, and only 45% of cultured neurons could be productively infected with eit

160 h were also blocked in studies using mice or cultured neurons deficient in the p47(phox) subunit of N

161 Here, we show that, in cultured neurons derived from LRRK2 G2019S transgenic mi

162 synaptophysin function, we expressed them in cultured neurons derived from synaptophysin knock-out mi

163 in neuroblastoma cells as well as in primary cultured neurons derived from Tg2576 mice.

164 The emergence of such network structure in cultured neurons, despite a lack of external input, poin

165 Here we show that mature cortical cultured neurons display a default state characterized b

166 Furthermore, while cultured neurons display Parkin-dependent axonal mitopha

167 ian-cultured cells, yeast, bacteria, primary cultured neurons, Drosophila melanogaster larval neuromu

168 The functional networks of cultured neurons exhibit complex network properties simi

169 The NGF-cultured neurons exhibited significant depolarization, b

170 Axons of cultured neurons experimentally depleted of kinesin-5 gr

171 ependent arrests is based on observations in cultured neurons exposed to artificial stimuli.

172 ofen, promotes neurite elongation in primary cultured neurons exposed to axonal growth inhibitors.

173 Cultured neurons exposed to depolarizing conditions reac

174 n, in ex vivo models of demyelination and in cultured neurons exposed to glutamate and tumor necrosis

175 cells stably expressing the CB1R and primary cultured neurons expressing endogenous CB1R, we show tha

176 Ca(2+) currents and synaptic facilitation in cultured neurons expressing exogenous CaV2.1 channels.

177 and reduced branching were observed both in cultured neurons expressing shRNA for ATXN3 and in those

178 Once cultured neurons expressing synaptopHluorin are availabl

179 We found that cultured neurons expressing transgenic (TG) NR3A display

180 In cultured neurons, fingolimod increases BDNF levels and c

181 WT cultured neurons fired only occasional single action pot

182 teracting with endogenous alpha-synuclein in cultured neurons following delivery via nanoparticles.

183 in vitro, and will contribute to the use of cultured neurons for drug discovery.

184 ove outcome, we compared immature and mature cultured neurons for susceptibility to three encephaliti

185 ogenous TNF-alpha showed higher toxicity for cultured neurons from A8-deficient than for those from w

186 high spatial and temporal resolution, using cultured neurons from brains of transgenic mice overexpr

187 tivity with RG108 and procainamide protected cultured neurons from excessive DNA methylation and apop

188 Additionally, LDN/OSU-0212320 protected cultured neurons from glutamate-mediated excitotoxic inj

189 Cultured neurons from HspB1-deficient mice were more sen

190 o degradation and binds to p38 MAPK protects cultured neurons from hypoxia-reoxygenation injury and r

191 tical imaging of pHluorin-tagged proteins in cultured neurons from knock-out mice lacking each protei

192 In cultured neurons from mice that harbor an RTT patient G

193 Additional studies in cultured neurons from MPS I mice showed that elevated sp

194 t P30 showed more dendritic branching, while cultured neurons from P0 PFC extended shorter neurites t

195 In slices and cultured neurons from rat hippocampus, we enhanced the t

196 ed the axonal transport of neurofilaments in cultured neurons from two different strains of dilute le

197 In contrast, cultured neurons from unc-31 mutants exhibited normal st

198 Further, immunostaining of cultured neurons from wild-type and SUMO1 knock-out mice

199 We analyzed cultured neurons from wild-type and Syn I,II,III(-/-) tr

200 tination of endogenous Bax comparing primary cultured neurons from WT and parkin KO mice and using mu

201 Moreover, in CPT1C KO cultured neurons, GluA1 synthesis after chemical long te

202 Experiments with cultured neurons have shown that when Dab1 is phosphoryl

203 Studies in cultured neurons have suggested that the position of the

204 in mammalian synapse function have relied on cultured neurons, heterologous expression systems, or me

205 euronal cell lines (PC12, GT1-7) and primary cultured neurons (hippocampal, cortex).

206 Moreover, its expression is upregulated in cultured neurons in response to various neurotoxins, inc

207 itochondrial fragmentation and cell death in cultured neurons in vitro, in mouse substantia nigra neu

208 vectors for genetic manipulation of primary cultured neurons in vitro.

209 pression of kindlin-1, but not kindlin-2, in cultured neurons increased axon growth on an inhibitory

210 CPT1C overexpression in primary hippocampal cultured neurons increased ceramide levels, whereas in C

211 Reelin stimulation of cultured neurons induces the extension of the Golgi into

212 Neurotrophins facilitate recruitment of cultured neurons into active networks, and it is this ac

213 of endogenous NPM1 oligomerization in these cultured neurons is toxic.

214 that loss of lysosomal Cacna1a in cerebellar cultured neurons leads to a failure of lysosomes to fuse

215 ow that increased E6AP expression in primary cultured neurons leads to a reduction in dendritic branc

216 tant role in growth and neurite extension of cultured neurons, localization of laminin in the brain h

217 Studies using cultured neuron models that faithfully recapitulate the

218 zinc reversibly reduced spiking frequency of cultured neurons most likely by suppressing Kv3 channels

219 In cultured neurons, native Ric-3 levels were higher than i

220 In primary cultured neurons, NES mutations increase nuclear accumul

221 We now show that in cultured neurons, neuroligin-1 overexpression increases

222 In cultured neurons, NMDA-induced superoxide production and

223 Experimental expression in cultured neurons of a short dysfunctional M1 polypeptide

224 Addition to cultured neurons of soluble teneurin-binding fragments o

225 ffects of early postnatal brain membranes or cultured neurons on MVECs were relieved significantly by

226 ripts at the RNA level after transduction of cultured neurons or after direct delivery and long-term

227 tochondria was studied either in dissociated cultured neurons or in brain slices, but not in the inta

228 tic turnover kinetics have been performed in cultured neurons outside the context of normal circuits,

229 In cultured neurons, PDN1 fused to a fluorescent protein in

230 We found that cultured neurons prepared from NR3A knock-out (KO) mice

231 We found that inhibition of kinesin-5 in cultured neurons prevents MTs from polarizing within gro

232 Knockdown of Miro2 in cultured neurons produced transport deficits identical t

233 ohibins or SVBP and/or inhibitor addition in cultured neurons reduced detyrosinated alpha-tubulin lev

234 Altered levels of SynDIG1 in cultured neurons result in striking changes in excitator

235 Two-photon microscopy of cultured neurons revealed large fluctuations in inner mi

236 ation of the effect of ectopic expression on cultured neurons revealed that increasing NPM1 is toxic

237 nsor for asynchronous release, we now use in cultured neurons short hairpin RNAs that suppress expres

238 MPAR endocytosis, siRNA against MAP1B in CA1 cultured neurons specifically blocked the DHPG-induced A

239 erologous expression systems and dissociated cultured neurons, studies in intact neurons revealed a s

240 dies using reporter proteins in unmyelinated cultured neurons suggest that an ankyrinG-binding motif

241 Studies in cultured neurons suggest that S-palmitoylation is requir

242 Addition of purified C3 cleavage products to cultured neurons suggested that C3b is responsible for t

243 ter stages enhanced mitochondrion numbers in cultured neurons, suggesting that CRMP5 modulated these

244 ctivity leads to spine loss from hippocampal-cultured neurons, suggesting that PKC may contribute to

245 ion of endogenous AMPA receptors (AMPARs) in cultured neurons suggests that LRRTMs maintain newly del

246 e conditional deletion of all three Mints in cultured neurons suppresses the accumulation of APP C-te

247 Cultured neurons survive well, develop extensive axonal

248 ucturally distinct and far more cytotoxic to cultured neurons than comparable LNOs made from Abeta(1-

249 We found in cultured neurons that both APP and Abeta are secreted ra

250 We show in cultured neurons that C1ql3 expression is activity depen

251 At synapses of cultured neurons that lack the two "neuronal" dynamins,

252 rapid depression of synaptic transmission in cultured neurons that transiently express CaV2.1 channel

253 In cultured neurons, the [Ca2+]i signals in the dendrites w

254 al mGlu4 receptor agonists, and, at least in cultured neurons, the action of low concentrations of ci

255 In cultured neurons, the addition of PrP(Sc) alters cell me

256 In cultured neurons, the E183V mutation reduces CaMKIIalpha

257 In cultured neurons, these v-SNARE mutations strongly inhib

258 Acute (minutes) exposure of cultured neurons to 10 nM clothianidin, but not imidaclo

259 Here, we performed studies on cultured neurons to ascertain whether these two proteins

260 Exposure of cultured neurons to fetal plasma or to secretions from t

261 Exposure of cultured neurons to micelles composed of these ceramide

262 Exposure of cultured neurons to oxygen/glucose deprivation resulted

263 study, we demonstrate that acute exposure of cultured neurons to soluble Abeta oligomers induces AMPA

264 rgeted molecular and genetic approaches with cultured neurons to study cell-intrinsic host defense pa

265 to analyses of alpha-synuclein expression in cultured neurons, to examinations of the effects of vira

266 models, flies expressing Abeta and mammalian cultured neurons treated with Abeta oligomers.

267 ction was measured by the ethidium method in cultured neurons treated with oxygen-glucose deprivation

268 hermore, we confirmed the pathway using both cultured neurons treated with recombinant TNFalpha in vi

269 The pathway was confirmed by using cultured neurons treated with recombinant TNFalpha in vi

270 sease brains (Braak Stage VI), as well as in cultured neurons under conditions of oxidative stress.

271 Massive cell death occurs in cultured neurons upon depleting syntaxin-1, Munc18-1, an

272 reted by 7PA2 cells caused synapse damage in cultured neurons via a PrP(C)-dependent process.

273 In fact, reducing levels of SF3B2 in tissue-cultured neurons was effective against neurotoxicity of

274 While the density of cultured neurons was not different between PS and contro

275 In hippocampal slices and cultured neurons we also observed significant beta4 expr

276 In cultured neurons we found that mutant huntingtin causes

277 l-length and mutant GFP-tagged Navbeta4 into cultured neurons, we determined that the AIS and nodal l

278 n the present study, using mouse hippocampal cultured neurons, we evaluated the significance of cathe

279 through analysis of FOXP2 ChIP-seq data from cultured neurons, we find strong overrepresentation of a

280 In cultured neurons, we found that the HIV coat protein gp1

281 mation of hyperphosphorylated tau (P-tau) in cultured neurons, we searched for P-tau by immunohistoch

282 y sufficient to delay axonal degeneration in cultured neurons, we sought to determine whether it was

283 Cultured neurons were prepared from rat pups on postnata

284 isease, causes dendritic and synapse loss in cultured neurons when expanded.

285 ly, we use a lentiviral expression system in cultured neurons, where we again find that eSIBR amiRNAs

286 h) enhanced voltage-gated Ca(2+) current in cultured neurons, whereas in vivo Fkbp1b knockdown by mi

287 enge previous conclusions based on work with cultured neurons, which suggested activity-dependent den

288 Treatments of cultured neurons with 3,4-dihydroxyphenylglycol (DHPG) o

289 Treatment of cultured neurons with exogenous ceramide reverted the KO

290 Transfection of cultured neurons with human GRIN2D cDNA harboring c.1999

291 n addition, Golgi fragmentation was found in cultured neurons with hyperactivity due to prolonged blo

292 Cultured neurons with no sign of MCMV infection showed a

293 Under identical global conditions, we cultured neurons with or without local astrocyte support

294 Conversely, treating cultured neurons with RNAi targeting alpha-takusan varia

295 s studies, we have shown that stimulation of cultured neurons with surrogate NCAM ligands leads to th

296 romoter was detected in approximately 90% of cultured neurons, with no preference for any neuronal su

297 antly suppressed ATF-3 expression in 48-hour-cultured neurons without effect on ATF-3 expression in S

298 f the channels triggers action potentials in cultured neurons without observable toxic effects.

299 (XBP1) in transgenic flies and in mammalian cultured neurons, yielding its active form, the transcri

300 ression, or were performed in cell lines and cultured neurons, yielding results difficult to translat