ライフサイエンスコーパス: cumulus

1 cumulus expansion in WT oocytectomized (OOX) cumulus.

2 ns, and the prevailing cloud type is shallow cumulus.

3 cause of a failure of sperm to penetrate the cumulus.

4 be regulated by capacitation and NO from the cumulus.

5 re assessed semiautomatically with software (Cumulus 4.0; University of Toronto, Toronto, Canada), an

6 ciated with larger breast density values for Cumulus ABD and CumulusV but not for Volpara and Quantra

7 Variability was higher for Cumulus ABD and CumulusV than for Volpara or Quantra.

8 was measured by using an area-based method (Cumulus ABD) and three automated volumetric methods (Cum

9 9, 1.33), and 1.64% (95% CI: 1.31, 1.39) for Cumulus ABD, CumulusV, Volpara, and Quantra, respectivel

10 imulated with progesterone (a product of the cumulus and thus encountered by sperm prior to fertiliza

11 and comprises three glomeruli, the globular cumulus and two toroidal structures.

12 tral follicle development but was low in the cumulus and virtually absent in the mural granulosa cell

13 them to overcome barriers and penetrate the cumulus and zona pellucida surrounding the egg.

14 ey must acquire more thrust to penetrate the cumulus and zona pellucida.

15 read centrally using a quantitative method (Cumulus) and its square-root metrics were analysed using

16 Cumulus cell apoptosis and matrix disassembly also occur

17 This indicates that oocytes regulate cumulus cell cholesterol biosynthesis by promoting the e

18 Cumulus cell clones initiated Oct4 expression at the cor

19 LH) induces maturational processes in oocyte-cumulus cell complexes (OCC) of preovulatory follicles t

20 hase 2 (Has2) mRNA was impaired in DM oocyte-cumulus cell complexes.

21 tes reduced levels of both KL transcripts in cumulus cell culture.

22 anges of matrix are temporally correlated to cumulus cell death.

23 cells, suggesting opposing effects of FSH on cumulus cell differentiation.

24 Cumulus cell expansion and resumption of meiosis with ge

25 coupled EP2 and EP4 receptor subtypes direct cumulus cell expansion and survival and oocyte meiotic m

26 In mouse granulosa cell and cumulus cell expansion assays, mouse GDF9 and human BMP1

27 strus cyclicity, estradiol biosynthesis, and cumulus cell expansion in vivo and reveal sites of actio

28 ivation in the cumulus cells, much increased cumulus cell expansion, and an accelerated severance of

29 uding follicular development, ovulation, and cumulus cell expansion.

30 Here we show that these factors control cumulus cell metabolism, particularly glycolysis and cho

31 dentified a 1605 nt cDNA sequence from mouse cumulus cell oocyte complexes (COCs) induced to expand i

32 back loop with the soma because it regulates cumulus cell replication.

33 s suggest that cAMP-elevating agents prevent cumulus cell senescence and allow them to continue to ex

34 etal fibroblast or terminally differentiated cumulus cell) and the recipient I2C cytoplasm, the recon

35 ct4-related genes was analyzed in individual cumulus cell-derived cloned blastocysts, only 62% correc

36 iffered between the embryonic stem cell- and cumulus cell-derived clones.

37 AMPK, was tested on denuded oocytes (DO) and cumulus cell-enclosed oocytes (CEO) maintained in meioti

38 n (AR), are potent inducers of maturation in cumulus cell-enclosed oocytes (CEO).

39 nthesized before ovulation, a process called cumulus cell-oocyte complex (COC) expansion.

40 hyaluronan-binding proteins, using parallel cumulus cell-oocyte complex (COC) extracts as positive c

41 The cumulus cell-oocyte complex (COC) matrix is an extended

42 has a critical role in the expansion of the cumulus cell-oocyte complex (COC), a process that is nec

43 dependent cross-linking of hyaluronan in the cumulus cell-oocyte complex during ovulation.

44 rthermore, the defective conformation of the cumulus cell-oocyte complex from the AR(-/-) females imp

45 Cultured TNFIP6-deficient cumulus cell-oocyte complexes also fail to expand when s

46 Cumulus cell-oocyte complexes fail to expand in TNFIP6-d

47 system, restore the expansion of Tnfip6-null cumulus cell-oocyte complexes in vitro, and rescue the f

48 Chondroitin interferes with the expansion of cumulus cell-oocyte complexes only when added with exoge

49 arides disperse cumulus cells from expanding cumulus cell-oocyte complexes with the same size specifi

50 en oocytes were microsurgically removed from cumulus cell-oocyte complexes, the isolated cumulus cell

51 rix with embedded cumulus cells, forming the cumulus cell.oocyte complex (COC) matrix.

52 us cells, lower levels were also detected in cumulus-cell-enclosed double mutant oocytes compared wit

53 cumulus cells after removal of oocytes from cumulus-cell-oocyte complexes.

54 II mouse oocytes (n = 240), with and without cumulus cells (CCs), were exposed for 4 hours to D-galac

55 ouble mutant cumulus cells, and in wild-type cumulus cells after removal of oocytes from cumulus-cell

56 GDF9 and BMP15 also regulate the function of cumulus cells after the preovulatory LH surge.

57 Embryonic keratinocytes and cumulus cells also gave rise to cloned mice.

58 Cumulus cells also promoted parthenogenetic activation t

59 layers: an outer layer of approximately 5000 cumulus cells and an inner, thick extracellular matrix,

60 acid ceramidase (AC), is expressed in human cumulus cells and follicular fluid, essential components

61 k matrix shell that is essentially devoid of cumulus cells and is enhanced upon COC expansion in vivo

62 in the development and function of both the cumulus cells and oocytes by assessing cumulus expansion

63 ntercellular metabolic cooperativity between cumulus cells and oocytes needed for energy production b

64 ls of cholesterol synthesis in double mutant cumulus cells and oocytes were partially restored by co-

65 junction-dependent communication between the cumulus cells and the oocyte as well as intact lipid raf

66 red in vitro, prolonging the vitality of the cumulus cells and the stability of the matrix from a few

67 successful ovulation, genes expressed in the cumulus cells and those that control cumulus expansion a

68 phic factor (BDNF) secreted by granulosa and cumulus cells as an ovarian factor stimulated by the pre

69 the expression of EGF-like growth factors in cumulus cells as well as a series of molecules downstrea

70 lesterol synthesis, were highly expressed in cumulus cells compared with oocytes; and oocytes, in the

71 e transition of preantral granulosa cells to cumulus cells competent to undergo expansion.

72 SH2-B(-/-) cumulus cells do not respond to either follicle-stimulat

73 in driving the differentiation of PAGCs into cumulus cells during the preantral to antral follicle tr

74 cumulus cell-oocyte complexes, the isolated cumulus cells exhibited decreased expression levels of g

75 -/- and Gdf9+/- Bmp15-/- (double mutant, DM) cumulus cells exhibited reduced levels of both glycolysi

76 reovulatory stages but becomes restricted to cumulus cells following antrum formation.

77 l granulosa cells (PAGCs) differentiate into cumulus cells following antrum formation.

78 Hyaluronan oligosaccharides disperse cumulus cells from expanding cumulus cell-oocyte complex

79 Thus, preantral granulosa cells differ from cumulus cells in CEEF-dependent processes downstream of

80 Results showed that the role of cumulus cells in MI arrest is dichotomous.

81 at the development and/or differentiation of cumulus cells in the DM, up to the stage of the preovula

82 aged mice that ceramide is translocated from cumulus cells into the adjacent oocyte and induces germ

83 Furthermore, the impaired expansion of cumulus cells may be partially associated with altered c

84 ysis and expression of Pfkp and Ldha mRNA in cumulus cells of wild-type (WT) mice.

85 rance of cytoplasmic projections between the cumulus cells outside the zona pellucida and the oocyte

86 Hyaluronan release and dispersion of the cumulus cells progressively occur after ovulation, paral

87 bitor, inhibited Pfkp and Ldha expression in cumulus cells promoted by paracrine oocyte factors.

88 Moreover, activation of MAPK in cumulus cells requires one or more paracrine factors fro

89 Steroid hormone progesterone released by cumulus cells surrounding the egg is a potent stimulator

90 The presence of Ke 6 protein within the cumulus cells surrounding the oocyte places it in a stra

91 Cumulus cells sustain the development and fertilization

92 Cumulus cells temporarily helped to sustain MI arrest, b

93 d to identify genes more highly expressed in cumulus cells than in mural granulosa cells of mouse ant

94 emarkable interaction between the oocyte and cumulus cells that is essential for gonadotropin-induced

95 lly, we show that CRISP1 is expressed by the cumulus cells that surround the egg and that fertilizati

96 female mice because of the inability of the cumulus cells to assemble their hyaluronan-rich extracel

97 ient in synthesizing cholesterol and require cumulus cells to provide products of the cholesterol bio

98 tial oocyte-secreted factors or of Fshb(-/-) cumulus cells to respond.

99 eased expression of Pfkp and Ldha mRNA in WT cumulus cells to the same levels as WT oocytes; however,

100 ficient mice and tested the ability of their cumulus cells to undergo mucification.

101 se decreases were prevented by culturing the cumulus cells with paracrine factors secreted by fully g

102 nteraction between the oocyte and encircling cumulus cells within a follicle, a unique venue for soma

103 Progesterone (a product of cumulus cells) also mobilises stored Ca(2+) in human spe

104 ei from somatic cells (Sertoli, neuronal and cumulus cells) taken from adult mice into enucleated mou

105 (-/-) and Bmp15(-/-) Gdf9(+/-) double mutant cumulus cells, and in wild-type cumulus cells after remo

106 rom the Taf4b null mice had fewer (P < 0.01) cumulus cells, and the oocytes were functionally abnorma

107 Cumulus cells, but not PAGCs, are competent to undergo e

108 (HA)-rich extracellular matrix with embedded cumulus cells, forming the cumulus cell.oocyte complex (

109 duced cholesterol synthesis in double mutant cumulus cells, lower levels were also detected in cumulu

110 different kinetics of MAPK activation in the cumulus cells, much increased cumulus cell expansion, an

111 nd GDF9, promote cholesterol biosynthesis in cumulus cells, probably as compensation for oocyte defic

112 and Amh, two transcripts highly expressed in cumulus cells, suggesting opposing effects of FSH on cum

113 d oocytes, in the absence of the surrounding cumulus cells, synthesized barely detectable levels of c

114 togen-activated protein kinase (MAPK) in the cumulus cells, thus suggesting that GDF9 and BMP15 also

115 the oocyte's companion granulosa cells, the cumulus cells, was investigated using fully grown oocyte

116 ncreases in expansion-related transcripts in cumulus cells, whereas growing oocytes of preantral foll

117 a cells to essentially the same levels as in cumulus cells.

118 preantral granulosa cells differentiate into cumulus cells.

119 x3 mRNAs to 17-96% of the levels observed in cumulus cells.

120 ed embryonic stem cells and freshly isolated cumulus cells.

121 it of AMPK were detected in both oocytes and cumulus cells.

122 vels of KL-1 mRNA in mural granulosa but not cumulus cells.

123 and the oocytes were often separate from the cumulus cells.

124 of Impdh and Npr2 and elevate cGMP levels in cumulus cells.

125 lopment are provided to oocytes by companion cumulus cells.

126 s and expression of Pfkp and Ldha mRNA in WT cumulus cells.

127 paracrine factors that promote glycolysis in cumulus cells.

128 rol synthesized de novo was reduced in these cumulus cells.

129 and FGFs cooperate to promote glycolysis in cumulus cells.

130 ween the oocyte and its surrounding somatic (cumulus) cells.

131 biomass burning season showed that scattered cumulus cloud cover was reduced from 38%in clean conditi

132 ested regions have well-developed dry season cumulus cloud fields.

133 ist static energy, and (ii) the use of three cumulus cloud types (congestus, stratiform, and deep con

134 ithin a 50- to 600-meter altitude band under cumulus clouds and then glide over kilometers at low ene

135 iding distances, birds regularly soar inside cumulus clouds to use their strong updraft, and they can

136 cal tropopause, ice crystal size in towering cumulus clouds, and aerosols associated with tropical bi

137 h in situ aerosol measurements below shallow cumulus clouds.

138 ild-type males to be detected within the egg/cumulus complex in the oviduct.

139 importance of the representation of shallow cumulus convection.

140 This may be possible by trapping of melt by cumulus crystal growth following melt drainage from an a

141 Chemical variation across developing cumulus crystals records changes in melt composition.

142 rientation for successful penetration of the cumulus during fertilization.

143 sed on the growing follicle and the ovulated cumulus-enclosed oocyte.

144 Moreover, induction of genes involved in cumulus expansion and follicle rupture is compromised in

145 iochemical events triggered by LH, including cumulus expansion and oocyte maturation.

146 GFR expression and to restore EGFR-dependent cumulus expansion and oocyte maturation.

147 GCs were tested for their ability to undergo cumulus expansion and upregulate expansion transcripts i

148 in the cumulus cells and those that control cumulus expansion are discussed.

149 Further, we found that BMP-15 induces cumulus expansion in mouse cumulus-oocyte complexes.

150 Cumulus expansion in vitro requires secretion of cumulus

151 ed genes (Ptx3, Has2, and Ptgs2) and promote cumulus expansion in vitro, whereas mouse BMP15 and huma

152 n, DM oocytes failed to enable FSH to induce cumulus expansion in WT oocytectomized (OOX) cumulus.

153 vation of MAPK required for inducing GVB and cumulus expansion is downstream of cAMP.

154 t can be fertilized in vitro, but defects in cumulus expansion prevent fertilization in vivo.

155 This aberrant cumulus expansion was not remedied by coculture with nor

156 ar matrix surrounding the oocyte that causes cumulus expansion, and for follicle rupture in vivo.

157 human chorionic gonadotropin also stimulated cumulus expansion, and this activity was attenuated by B

158 for cyclooxygenase 2, an enzyme required for cumulus expansion, are increased.

159 We found that cumulus expansion, as well as the expression of hyaluron

160 enes whose products are necessary for normal cumulus expansion, Has2 and Ptgs2.

161 h the cumulus cells and oocytes by assessing cumulus expansion, oocyte maturation, fertilization, and

162 9 and human BMP15 homodimers can up-regulate cumulus expansion-related genes (Ptx3, Has2, and Ptgs2)

163 lls may be partially associated with altered cumulus expansion-related transcripts that are regulated

164 lay delayed or reduced oocyte maturation and cumulus expansion.

165 y abolished the effect of BMP-15 in inducing cumulus expansion.

166 3 mRNA levels, all of which are required for cumulus expansion.

167 xin 3, all of which are necessary for normal cumulus expansion.

168 promoting the resumption of meiosis, governs cumulus expansion.

169 ne factors from the oocyte to induce GVB and cumulus expansion; MAPK activation alone is not sufficie

170 lus expansion in vitro requires secretion of cumulus-expansion enabling factors (CEEFs) by the oocyte

171 anization and stabilization of the expanding cumulus extracellular matrix (cECM) following an ovulato

172 P6 is a key catalyst in the formation of the cumulus extracellular matrix and indispensable for femal

173 The formation of the hyaluronan-rich cumulus extracellular matrix is crucial for female ferti

174 roup of BMP secreting mesenchymal cells (the cumulus) functions as an organizer of the dorsoventral a

175 tial EGFR-regulated events: expansion of the cumulus granulosa cell layer that encloses the oocyte an

176 abnormal polar bodies, are detached from the cumulus granulosa cell layer, and display spindle and nu

177 preovulatory follicles were co-cultured with cumulus granulosa cells, Amh expression was increased at

178 to be required for progesterone synthesis in cumulus granulosa cells.

179 to SLLP1 on in vitro fertilization with both cumulus intact and zona-free eggs, and the definition of

180 Treatment of cumulus intact oocytes with either recmSLLP1 or its anti

181 e transcription factor Pt-Ets4 is needed for cumulus integrity, dorsoventral patterning and for the a

182 he Spemann organizer, transplantation of the cumulus is able to induce a secondary axis in spiders.

183 l simulations, the daytime clearing of trade cumulus is hastened and intensified by solar heating in

184 Mucification of the cumulus layer around the oocyte is an obligatory process

185 After hormone-induced ovulation, cumulus masses were present in the oviducts of homozygou

186 y the loss of hyaluronan-linked HCs from the cumulus matrix and the appearance of oligosaccharide-lin

187 hyaluronan is a prerequisite for the correct cumulus matrix assembly and hyaluronan oligosaccharides

188 The impaired cumulus matrix formation is due to the lack of covalent

189 s indicate that appropriate formation of the cumulus matrix is essential for successful ovulation, ge

190 g the expansion of the COC and providing the cumulus matrix with its required viscoelastic properties

191 mimicking penetration into cervical mucus or cumulus matrix) was enhanced by activation of CatSper.

192 present at micromolar concentrations in the cumulus matrix, which surrounds mammalian oocytes.

193 ertile due to the lack of a correctly formed cumulus matrix.

194 ajor role in the stabilization of the murine cumulus matrix.

195 h percentage of enucleated oocytes receiving cumulus nuclei developed in vitro.

196 cumulated in the extracellular matrix of the cumulus oocyte complex (COC) during the process of matri

197 thma and arthritis) and in the matrix of the cumulus oocyte complex.

198 Despite similarities in the morphology of cumulus oocyte complexes (COCs) expanding in vivo and in

199 ing oocyte maturation in vivo since ovulated cumulus oocyte complexes collected from FYN (-/-) mice i

200 However, the ovulated cumulus oocyte complexes from the Taf4b null mice had fe

201 cle breakdown (GVBD) prior to ovulation, the cumulus-oocyte complex was markedly disrupted and the oo

202 ammatory conditions and in the matrix of the cumulus-oocyte complex, the polysaccharide hyaluronan (H

203 ," improves nuclear maturation of oocytes in cumulus-oocyte complexes derived from immature pig ovari

204 As they progress in culture, the FLI-matured cumulus-oocyte complexes display distinctly different ki

205 t surround the egg and that fertilization of cumulus-oocyte complexes from CRISP1 knockout females is

206 at BMP-15 induces cumulus expansion in mouse cumulus-oocyte complexes.

207 The cumulus oophorus of large antral follicles undergoes exp

208 e oocyte and expansion (mucification) of the cumulus oophorus.

209 ains have been demonstrated in the ovulatory cumulus oophorus.

210 ccur during capacitation/transit through the cumulus, prior to any physical contact between the sperm

211 ed all images for mammographic density using Cumulus software (Sunnybrook Health Sciences Centre, Tor

212 unknown which factors are needed to activate cumulus specific gene expression.

213 utive and inducible NOS in human oviduct and cumulus (the cellular layer investing the oocyte).