ライフサイエンスコーパス: cumulus cell

1 it of AMPK were detected in both oocytes and cumulus cells.

2 vels of KL-1 mRNA in mural granulosa but not cumulus cells.

3 and the oocytes were often separate from the cumulus cells.

4 of Impdh and Npr2 and elevate cGMP levels in cumulus cells.

5 lopment are provided to oocytes by companion cumulus cells.

6 s and expression of Pfkp and Ldha mRNA in WT cumulus cells.

7 paracrine factors that promote glycolysis in cumulus cells.

8 rol synthesized de novo was reduced in these cumulus cells.

9 and FGFs cooperate to promote glycolysis in cumulus cells.

10 a cells to essentially the same levels as in cumulus cells.

11 preantral granulosa cells differentiate into cumulus cells.

12 x3 mRNAs to 17-96% of the levels observed in cumulus cells.

13 ed embryonic stem cells and freshly isolated cumulus cells.

14 ween the oocyte and its surrounding somatic (cumulus) cells.

15 ouble mutant cumulus cells, and in wild-type cumulus cells after removal of oocytes from cumulus-cell

16 GDF9 and BMP15 also regulate the function of cumulus cells after the preovulatory LH surge.

17 Embryonic keratinocytes and cumulus cells also gave rise to cloned mice.

18 Cumulus cells also promoted parthenogenetic activation t

19 Progesterone (a product of cumulus cells) also mobilises stored Ca(2+) in human spe

20 layers: an outer layer of approximately 5000 cumulus cells and an inner, thick extracellular matrix,

21 acid ceramidase (AC), is expressed in human cumulus cells and follicular fluid, essential components

22 k matrix shell that is essentially devoid of cumulus cells and is enhanced upon COC expansion in vivo

23 in the development and function of both the cumulus cells and oocytes by assessing cumulus expansion

24 ntercellular metabolic cooperativity between cumulus cells and oocytes needed for energy production b

25 ls of cholesterol synthesis in double mutant cumulus cells and oocytes were partially restored by co-

26 junction-dependent communication between the cumulus cells and the oocyte as well as intact lipid raf

27 red in vitro, prolonging the vitality of the cumulus cells and the stability of the matrix from a few

28 successful ovulation, genes expressed in the cumulus cells and those that control cumulus expansion a

29 etal fibroblast or terminally differentiated cumulus cell) and the recipient I2C cytoplasm, the recon

30 (-/-) and Bmp15(-/-) Gdf9(+/-) double mutant cumulus cells, and in wild-type cumulus cells after remo

31 rom the Taf4b null mice had fewer (P < 0.01) cumulus cells, and the oocytes were functionally abnorma

32 Cumulus cell apoptosis and matrix disassembly also occur

33 phic factor (BDNF) secreted by granulosa and cumulus cells as an ovarian factor stimulated by the pre

34 the expression of EGF-like growth factors in cumulus cells as well as a series of molecules downstrea

35 Cumulus cells, but not PAGCs, are competent to undergo e

36 II mouse oocytes (n = 240), with and without cumulus cells (CCs), were exposed for 4 hours to D-galac

37 This indicates that oocytes regulate cumulus cell cholesterol biosynthesis by promoting the e

38 Cumulus cell clones initiated Oct4 expression at the cor

39 lesterol synthesis, were highly expressed in cumulus cells compared with oocytes; and oocytes, in the

40 e transition of preantral granulosa cells to cumulus cells competent to undergo expansion.

41 LH) induces maturational processes in oocyte-cumulus cell complexes (OCC) of preovulatory follicles t

42 hase 2 (Has2) mRNA was impaired in DM oocyte-cumulus cell complexes.

43 tes reduced levels of both KL transcripts in cumulus cell culture.

44 anges of matrix are temporally correlated to cumulus cell death.

45 ct4-related genes was analyzed in individual cumulus cell-derived cloned blastocysts, only 62% correc

46 iffered between the embryonic stem cell- and cumulus cell-derived clones.

47 cells, suggesting opposing effects of FSH on cumulus cell differentiation.

48 SH2-B(-/-) cumulus cells do not respond to either follicle-stimulat

49 in driving the differentiation of PAGCs into cumulus cells during the preantral to antral follicle tr

50 AMPK, was tested on denuded oocytes (DO) and cumulus cell-enclosed oocytes (CEO) maintained in meioti

51 n (AR), are potent inducers of maturation in cumulus cell-enclosed oocytes (CEO).

52 us cells, lower levels were also detected in cumulus-cell-enclosed double mutant oocytes compared wit

53 cumulus cell-oocyte complexes, the isolated cumulus cells exhibited decreased expression levels of g

54 -/- and Gdf9+/- Bmp15-/- (double mutant, DM) cumulus cells exhibited reduced levels of both glycolysi

55 Cumulus cell expansion and resumption of meiosis with ge

56 coupled EP2 and EP4 receptor subtypes direct cumulus cell expansion and survival and oocyte meiotic m

57 In mouse granulosa cell and cumulus cell expansion assays, mouse GDF9 and human BMP1

58 strus cyclicity, estradiol biosynthesis, and cumulus cell expansion in vivo and reveal sites of actio

59 ivation in the cumulus cells, much increased cumulus cell expansion, and an accelerated severance of

60 uding follicular development, ovulation, and cumulus cell expansion.

61 l granulosa cells (PAGCs) differentiate into cumulus cells following antrum formation.

62 reovulatory stages but becomes restricted to cumulus cells following antrum formation.

63 (HA)-rich extracellular matrix with embedded cumulus cells, forming the cumulus cell.oocyte complex (

64 Hyaluronan oligosaccharides disperse cumulus cells from expanding cumulus cell-oocyte complex

65 Thus, preantral granulosa cells differ from cumulus cells in CEEF-dependent processes downstream of

66 Results showed that the role of cumulus cells in MI arrest is dichotomous.

67 at the development and/or differentiation of cumulus cells in the DM, up to the stage of the preovula

68 aged mice that ceramide is translocated from cumulus cells into the adjacent oocyte and induces germ

69 duced cholesterol synthesis in double mutant cumulus cells, lower levels were also detected in cumulu

70 Furthermore, the impaired expansion of cumulus cells may be partially associated with altered c

71 Here we show that these factors control cumulus cell metabolism, particularly glycolysis and cho

72 different kinetics of MAPK activation in the cumulus cells, much increased cumulus cell expansion, an

73 ysis and expression of Pfkp and Ldha mRNA in cumulus cells of wild-type (WT) mice.

74 dentified a 1605 nt cDNA sequence from mouse cumulus cell oocyte complexes (COCs) induced to expand i

75 nthesized before ovulation, a process called cumulus cell-oocyte complex (COC) expansion.

76 hyaluronan-binding proteins, using parallel cumulus cell-oocyte complex (COC) extracts as positive c

77 The cumulus cell-oocyte complex (COC) matrix is an extended

78 has a critical role in the expansion of the cumulus cell-oocyte complex (COC), a process that is nec

79 dependent cross-linking of hyaluronan in the cumulus cell-oocyte complex during ovulation.

80 rthermore, the defective conformation of the cumulus cell-oocyte complex from the AR(-/-) females imp

81 Cultured TNFIP6-deficient cumulus cell-oocyte complexes also fail to expand when s

82 Cumulus cell-oocyte complexes fail to expand in TNFIP6-d

83 system, restore the expansion of Tnfip6-null cumulus cell-oocyte complexes in vitro, and rescue the f

84 Chondroitin interferes with the expansion of cumulus cell-oocyte complexes only when added with exoge

85 arides disperse cumulus cells from expanding cumulus cell-oocyte complexes with the same size specifi

86 en oocytes were microsurgically removed from cumulus cell-oocyte complexes, the isolated cumulus cell

87 cumulus cells after removal of oocytes from cumulus-cell-oocyte complexes.

88 rix with embedded cumulus cells, forming the cumulus cell.oocyte complex (COC) matrix.

89 rance of cytoplasmic projections between the cumulus cells outside the zona pellucida and the oocyte

90 nd GDF9, promote cholesterol biosynthesis in cumulus cells, probably as compensation for oocyte defic

91 Hyaluronan release and dispersion of the cumulus cells progressively occur after ovulation, paral

92 bitor, inhibited Pfkp and Ldha expression in cumulus cells promoted by paracrine oocyte factors.

93 back loop with the soma because it regulates cumulus cell replication.

94 Moreover, activation of MAPK in cumulus cells requires one or more paracrine factors fro

95 s suggest that cAMP-elevating agents prevent cumulus cell senescence and allow them to continue to ex

96 and Amh, two transcripts highly expressed in cumulus cells, suggesting opposing effects of FSH on cum

97 Steroid hormone progesterone released by cumulus cells surrounding the egg is a potent stimulator

98 The presence of Ke 6 protein within the cumulus cells surrounding the oocyte places it in a stra

99 Cumulus cells sustain the development and fertilization

100 d oocytes, in the absence of the surrounding cumulus cells, synthesized barely detectable levels of c

101 ei from somatic cells (Sertoli, neuronal and cumulus cells) taken from adult mice into enucleated mou

102 Cumulus cells temporarily helped to sustain MI arrest, b

103 d to identify genes more highly expressed in cumulus cells than in mural granulosa cells of mouse ant

104 emarkable interaction between the oocyte and cumulus cells that is essential for gonadotropin-induced

105 lly, we show that CRISP1 is expressed by the cumulus cells that surround the egg and that fertilizati

106 togen-activated protein kinase (MAPK) in the cumulus cells, thus suggesting that GDF9 and BMP15 also

107 female mice because of the inability of the cumulus cells to assemble their hyaluronan-rich extracel

108 ient in synthesizing cholesterol and require cumulus cells to provide products of the cholesterol bio

109 tial oocyte-secreted factors or of Fshb(-/-) cumulus cells to respond.

110 eased expression of Pfkp and Ldha mRNA in WT cumulus cells to the same levels as WT oocytes; however,

111 ficient mice and tested the ability of their cumulus cells to undergo mucification.

112 the oocyte's companion granulosa cells, the cumulus cells, was investigated using fully grown oocyte

113 ncreases in expansion-related transcripts in cumulus cells, whereas growing oocytes of preantral foll

114 se decreases were prevented by culturing the cumulus cells with paracrine factors secreted by fully g

115 nteraction between the oocyte and encircling cumulus cells within a follicle, a unique venue for soma